全文获取类型
收费全文 | 65554篇 |
免费 | 5417篇 |
国内免费 | 4453篇 |
专业分类
耳鼻咽喉 | 438篇 |
儿科学 | 1808篇 |
妇产科学 | 905篇 |
基础医学 | 12612篇 |
口腔科学 | 1334篇 |
临床医学 | 5704篇 |
内科学 | 9730篇 |
皮肤病学 | 916篇 |
神经病学 | 4355篇 |
特种医学 | 1559篇 |
外国民族医学 | 29篇 |
外科学 | 3837篇 |
综合类 | 13415篇 |
现状与发展 | 22篇 |
一般理论 | 1篇 |
预防医学 | 3562篇 |
眼科学 | 1108篇 |
药学 | 4651篇 |
5篇 | |
中国医学 | 1447篇 |
肿瘤学 | 7986篇 |
出版年
2024年 | 106篇 |
2023年 | 652篇 |
2022年 | 1356篇 |
2021年 | 2024篇 |
2020年 | 1796篇 |
2019年 | 1706篇 |
2018年 | 1632篇 |
2017年 | 1814篇 |
2016年 | 2112篇 |
2015年 | 2219篇 |
2014年 | 3430篇 |
2013年 | 4368篇 |
2012年 | 3660篇 |
2011年 | 4389篇 |
2010年 | 3582篇 |
2009年 | 3590篇 |
2008年 | 3864篇 |
2007年 | 4146篇 |
2006年 | 3947篇 |
2005年 | 3683篇 |
2004年 | 3303篇 |
2003年 | 2856篇 |
2002年 | 2508篇 |
2001年 | 2287篇 |
2000年 | 1927篇 |
1999年 | 1634篇 |
1998年 | 1408篇 |
1997年 | 1187篇 |
1996年 | 841篇 |
1995年 | 728篇 |
1994年 | 577篇 |
1993年 | 392篇 |
1992年 | 311篇 |
1991年 | 254篇 |
1990年 | 219篇 |
1989年 | 159篇 |
1988年 | 124篇 |
1987年 | 115篇 |
1986年 | 79篇 |
1985年 | 88篇 |
1984年 | 62篇 |
1983年 | 30篇 |
1982年 | 53篇 |
1981年 | 42篇 |
1980年 | 28篇 |
1979年 | 35篇 |
1978年 | 14篇 |
1977年 | 19篇 |
1976年 | 23篇 |
1969年 | 9篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
51.
人睫状神经营养因子结构和功能的研究 总被引:4,自引:1,他引:3
制备高活性的重组人睫状神经营养因子,并研究其生物学功能。方法:应用大肠杆菌表达hCNTF,用片段插入和法研究其结构与功能关系;切断大鼠骨神经,局部及皮下给予CNTF,应用辣根过氧化物酶逆行追踪技术显示再生轴突通过修复部位的胞体。结果;hCNTF分子中α-螺旋结构的维持对其生物活性十分重要C端松散地其生物活性贡献不大,D螺旋中后段可能与生物活性有密切关系; 相似文献
52.
The extracellular electrophysiological properties of neurons in the laterodorsal tegmental nucleus (LDT), a major source of cholinergic afferents to the thalamus, were studied in chloral hydrate-anesthetized rats. A combination of antidromic activation from the thalamus and histological verification of recording sites was used to correlate the identity of extracellular recordings in the rat LDT with cholinergic neurons in that region. All neurons antidromically activated by stimulation of the anteroventral thalamus were histologically verified to be within clusters of cholinergic (NADPH-d-positive) cells in the LDT or in the adjacent nucleus locus coeruleus (LC). The thalamically projecting LDT neurons had a homogeneous neurophysiological profile consisting of long duration action potentials (mean = 2.5 ms), slow conduction velocities (mean = 0.78 m/s), and lengthy chronaxie values (mean = 0.725 ms). The appearance and axonal characteristics of these neurons resembled those of noradrenergic LC neurons, but the two populations exhibited substantially different spontaneous activity patterns and sensory responsiveness. These characteristics may be useful in the preliminary identification of putative cholinergic neurons in vivo, and thereby provide a foundation for exploring the neuropharmacology, afferent modulation, sensory responsiveness and behavioral correlates of the brainstem cholinergic system. 相似文献
53.
Prediction of steady state bioequivalence relationships using single dose data II-nonlinear kinetics
A J Jackson 《Biopharmaceutics & drug disposition》1989,10(5):489-503
Two nonlinear pharmacokinetic models were simulated to investigate the relationship between single and multiple dose bioequivalency parameters for drugs such as phenytoin and propranolol which exhibit either saturable elimination kinetics or a capacity limited first pass effect. Mean Tmax, Cmax and area under the plasma-concentration time curve values from 0 to infinity (AUC 0-infinity) were compared after a single and multiple dose(s) of a test or reference drug. The aim was to determine if there were systematic changes in the limits of the single dose confidence interval at steady state that would limit the usefulness of confidence intervals following a single dose in accurately predicting bioavailability following multiple dosing. The 90 per cent confidence interval expressed as a percentage of the reference mean for Tmax, Cmax, and AUC 0-infinity showed model dependent changes from single to multiple dosing in response to the level of data error and changes in absorption. Changes in clearance also seemed to have a marked effect on the observed limits of the single and multiple dose confidence intervals especially for Cmax which showed a characteristic change in the intervals as a function of the clearance ratio. The model used to describe phenytoin had confidence intervals for Cmax and AUC 0-infinity from single to multiple dosing that were similar to that seen for the experimental data. However, the model predictions for Tmax confidence intervals following single and multiple dosing was at variance with the experimental data for formulations A and B. 相似文献
54.
Summary A new modification of microvenous anastomosis, which has increased patency rates while simultaneously decreasing the difficulty of the procedure, is presented in this paper. The primary purpose of this study was to compare the classical and the temporary stent techniques of microvenous anastomosis. Because of problems such as mixing and tangling of strings during insertion and tying of the last four sutures while applying the temporary stent technique, we decided to modify the procedure. The silastic tube was removed through an incision (venotomy), distant from the actual suture line. This modified technique and the other above mentioned techniques were carried out on rat femoral veins. The results indicate that this modification has increased patency rates, shortened the time of anastomosis and facilitated the procedure. 相似文献
55.
Etiological heterogeneity in Hodgkin's disease: HLA linked and unlinked determinants of susceptibility independent of histological concordance 总被引:2,自引:0,他引:2
Forty-one multiplex families, from published sources and new data from the National Cancer Institute, segregating for Hodgkin's disease and HLA, have been studied. A reanalysis of these data strongly suggests a recessive mode of inheritance for susceptibility to Hodgkin's disease. The HLA haplotype sharing data between affected relatives demonstrate that approximately 60% of cases in multiplex families are due to an HLA-linked susceptibility gene, the remaining 40% being due to other familial factors. The data clearly support the hypothesis of etiological heterogeneity for Hodgkin's disease, with both HLA-linked and HLA-unlinked factors being responsible. Finally, there is an increased concordance of histological types between affected relatives, but this concordance seems independent of HLA sharing. 相似文献
56.
目的研究不同剂量60Co γ射线辐照全血在保存期内红细胞CR1分子数目的变化.方法应用酶联法定量测定经15~35GY五个照射剂量辐照全血在保存期内红细胞CR1分子数目.结果照射后1d,15~25GY剂量组红细胞CR1分子数目相互间比较及分别与对照组比较无明显差异(P>0.05);经30、35GY辐照红细胞CR1分子数目分别与其他剂量组和对照组比较有明显差异(P<0.05~0.01).另外,随着保存期的延长,各剂量组和对照组红细胞CR1分子数目呈阶梯式下降,尤其在照射后3d,30和35GY剂量组红细胞CR1分子数目接近于照射后7d水平,二者相互比较无明显差异(P>0.05).结论在一定范围内(15~25GY)60Co γ射线剂量辐照对红细胞CR1分子数目无明显影响. 相似文献
57.
目的观察Agilent 2100 Bioanalyzer 芯片分析系统(以下简称Bioanalyzer)在基因差异表达研究中的应用。方法应用限制性显示技术分别从正常和热休克处理后的酿酒酵母细胞中分离出cDNA片段,然后再用Bioanalyzer和传统的琼脂糖凝胶电泳技术对RD-PCR产物进行检测分析。结果Bioanalyzer能更快速、敏感地分离和显示差异表达的基因片段,并且通过对差异片段进行定量比较,发现了数个表达有明显差异的基因片段。结论Bioanalyzer在基因差异表达研究中具有重要的应用价值。 相似文献
58.
Zhe-ping HUANG Jian WANG Wei-xiong SHEN Ping HUANG Jia-ke TSO Qing-xiang SHEN 《生殖与避孕(英文版)》2002,13(4)
Objective To identify genes that may be related to embryo implantation Materials & Methods The PCR subtraction technique was applied at implantation and inter-plantation sites on day 4. 5 of pregnancy in mice. Two novel Expressed Sequence Tags (ESTs ), EST8 and EST81 were identified; their expression in tissues was analyzed by Northern blotting, and their full-length cDNAs were synthesized by PCR.Results We found that these two novel ESTs (EST8and EST81) were noticeably expressed in implantation site in the mouse on day 4. 5 of pregnancy. EST8 was expressed at high level in livers and implantation sites of the mice, while at low level in ovaries and inter-plantation sites. EST81 was predominantly expressed in implantation site and ovary, and at low level in all other tissues. Their complete cDNAs, 1 665bp and 1 264 bp respectively, were synthesized by using PCR.Conclusion The two full-length cDNAs were responsible for embryo implantation,and their functions need to be further studied. 相似文献
59.
汪希 《上海第二医科大学学报》2002,14(2)
Objective In order to improve the in vivo gene transfer into the heart muscle, we have designed a ECG-synchronized microinjection system that allows sequential gene delivery to the myocardium.Methods A cannula was introduced into the right carotid artery of the Wistar rat under general anesthesia.With the ECG-synchronized injection during diastole, the genetic vector (Ad CMV lacZ ) infusion was performed with various concentrations( l07 ~ l010pfu ) and different frequency ( the ratio of heart beats per injection from 1: 1 to 4: 1 ). The hearts of the rats were removed after 7 days for histological examination. Results Best results were obtained with a total vector amount of l09 pfu and a good ratio 3: 1 between heart frequency and injection frequency. The transfection efficiency was increased by use of vasodilators and by an increase of vascular permeability. No signs of myocardial ischemia or ventricular arrythmia were observed. Conclusion We have established a novel and safe method for in vivo gene transfer into the heart. Transgene expression suggests that this method may be useful technique to study cardiac function of treat cardiac diseases by means of gene theratpy. 相似文献
60.