三种泻心汤及其类方不同配伍对正常大鼠胃粘液成分的影响

目的:探讨半夏泻心汤及其类方不同配伍对正常大鼠粘液含量的影响.方法:用均匀设计法对半夏泻心汤及其类方进行组方药量变化,以胃粘液含量为药理学指标,对所得的药理实验数据进行逐步回归分析.结果:与胃粘液含量关联度最大的为黄连,可以显著降低其含量,其次对半夏、大枣和甘草的交互项,可增加胃粘液含量.药物之间存在复杂的交互作用.结论:均匀设计为研究类方配伍提供了一种可供参考的试验设计方法.     

蒙古族验方北沙参四味汤研究进展及药效物质基础预测

蒙成药经典验方北沙参四味汤历史悠久,有清肺解热、止咳祛痰的功效,临床多用来治疗肺炎、支气管炎等疾病,疗效确切,具有潜在的开发价值。本文基于化学成分、药理作用、质量控制和临床研究,综述了北沙参四味汤的研究现状并预测分析其潜在的药效物质基础,为其后续相关研究提供参考。     

转FL、GM-CSF基因的人骨髓基质细胞促进脐血CD34+细胞体外扩增

研究转FL、GM-CSF基因的基质细胞对脐血CD34+细胞的扩增效应.将转FL、GM-CSF基因的入骨髓基质细胞系与脐血CD34+细胞共培养,观察细胞总数、CD34+细胞数、CFU-GM的变化情况.培养到第4周时,第(4)组(SCF+IL-3+IL-6+GM-CSF+FL)和第(8)组(HFCL/hGM-CSF+HFCL/hFL+SCF+IL-3+IL-6)的细胞总数增加到最大,分别扩增了717±24.47和709±63.63,第1周,第(5)组(HFCL+SCF+IL-3+IL-6)扩增了10.5±2.08倍,较第(8)组减少(P＜0.05).第1周时,CD34+细胞总数第(4)组和第(8)组分别扩增了8.44倍和11.5倍(P＜0.05),CD34+细胞百分率第(7)组(FCL/hFL+SCF+IL-3+II,-6)为50.2%,第(6)组(HFCL/hGM-CSF+SCF+IL-3+IL-6)为28.95%(P＜0.01).第2周,各组CFU-GM增加显著,以第(4)组和第(8)组增加最为明显,以后随扩增时间延长,造血细胞集落数、集落体积逐渐减少.表明转FL、GM-CSF基因的基质细胞,能有效的协同其他细胞因子对脐血CD34+细胞产生明显的扩增作用,能显著改变基质细胞造血功能.     

Effects of chemical mediators of anaphylaxis on ciliary function

We assessed the effects of selected chemical mediators of anaphylaxis on CBF in vitro. Ciliated epithelial cells were obtained from the trachea of conscious sheep with a cytology brush and suspended in a perfusion chamber containing KH. Ciliary activity was viewed microscopically and recorded on videotape for subsequent slow-motion analysis of CBF. Prostaglandin E1 (10(-8) M to 10(-6) M), prostaglandin E2 (10(-10) M to 10(-6) M), and leukotriene-C4 (10(-8) M) increased CBF between 7% and 33%. Histamine caused ciliostimulation only at the relatively high concentrations above 10(-5) M (7% increase in CBF), whereas prostaglandin F2 alpha (10(-10) M and 10(-6) M) was without effect. In no preparation was ciliary discoordination observed. These findings indicate that several chemical mediators of anaphylaxis stimulate CBF and that the previously described impairment of mucociliary transport in stable allergic asthma or antigen-induced bronchoconstriction is probably not caused by a primary alteration of ciliary function.     

Airway responses to antigen challenge in allergic rhinitis and allergic asthma

The density dependence of the maximum expiratory flow-volume curve, functional residual capacity (FRC), and specific airway conductance (SGaw) were determined before and during bronchial provocation with ragweed extract in 27 subjects with ragweed hypersensitivity and a history of either bronchial asthma (16 subjects) or allergic rhinitis (11 subjects). Mean baseline SGaw was significantly lower while mean volume of isoflow (Visov) and FrC were significantly higher in subjects with bronchial asthma. During antigen challenge, 10 of 16 subjects with bronchial asthma (63%) and five of 11 subjects with allergic rhinitis (45%) showed a greater than 35% decrease in SGaw ("reactors"): mean relative decreases in SGaw from baseline were 46% and 53%, respectively. The remaining subjects showed a less than 35% decrease in SGaw ("nonreactors") with mean relative decreases of 9% (allergic asthma) and 6% (allergic rhinitis). Mean Visov increased in all subjects with bronchial asthma and in eight of 11 subjects with allergic rhinitis. A significant increase in FRC (6%) was seen only in the "reactors" with bronchial asthma. Following antigen challenge, the beta adrenergic agonist, isoetharine, increased SGaw and decreased Visov. We conclude that in asymptomatic subjects with ragweed hypersensitivity, (1) central and peripheral airway function is more abnormal in subjects with bronchial asthma than in subjects with allergic rhinitis, (2) subjects of both groups show quantitatively and qualitatively comparable airway responses during antigen challenge with a decrease in SGaw or an increase in Visov, possibly representing increase in central and/or peripheral airflow resistance, respectively, (3) Visov may be a more sensitive indicator of airway response to antigen challenge than SGaw, and (4) the bronchodilator effects of a beta adrenergic agonist on antigen-induced bronchospasm are similar in both groups.     

小陷胸汤加味含药血清对人脐静脉内皮细胞分泌NO/ET-1的调节作用

目的:探讨小陷胸汤加味中药方对血管内皮细胞的保护作用。方港:建立ox-LDL损伤人脐静脉内皮细胞株(ECV-304)模型,用小陷胸汤加味含药血清处理模型,并用放免和硝酸酶还原法在药物干预6h和24h后检测细胞上清液中ET-1和NO含量。结果:100 μg/ml的ox-LDL可损伤血管内皮细胞并导致其分泌NO和ET-1功能失调,小陷胸汤加味含药血清通过影响NO/ET-1的分泌而明显改善此失调状态。结论:小陷胸汤加味中药通过调节NO/ET-1水平显著拮抗ox-LDL对血管内皮细胞损害,具有防治AS的作用。     

八珍汤对血虚模型小鼠造血调控因子影响的实验研究

探讨八珍汤对由环磷酰胺引起的小鼠骨髓造血功能抑制的调控作用。采用环磷酰胺致小鼠血虚模型 ,测定八珍汤对骨髓抑制小鼠外周血象及其细胞因子产生的影响。结果表明 ,八珍汤对环磷酰胺所致血虚模型小鼠骨髓细胞有促进增殖作用 ;经八珍汤诱导制备的巨噬细胞、脾细胞、肺条件培养液和骨骼肌条件培养液能促进血虚模型小鼠骨髓细胞增殖 ,促进血虚模型小鼠骨髓基质细胞分泌肿瘤坏死因子 (TNF)。八珍汤对环磷酰胺所致化疗损伤的造血调控作用可能与直接或间接刺激造血微环境的基质细胞分泌正性和负性造血生长因子有关。     

从氧自由基、一氧化氮探讨四逆汤抗急性失血性休克的肝脏机制

目的:从氧自由基、一氧化氮探讨四逆汤抗急性失血性休克的肝脏机制。方法:复制急性失血性休克大鼠模型, 分为假手术对照组;单纯休克模型组;休克+生理盐水复苏组;休克+四逆注射液复苏组。四逆注射液(浓度1000g生药/L), 剂量0.1mL/200g大鼠。用生理盐水或四逆注射液治疗3h后处死动物并取组织。测定各组肝脏超氧化物歧化酶(SOD)活性、丙二醛(MDA)水平, 一氧化氮(NO)水平。采用免疫组化染色法, 观察诱导型一氧化氮合酶(iNOS)在肝细胞中的变化特点。RT-PCR观测肝细胞iNOS和内皮源性一氧化氮合酶(eNOS)基因表达的变化。结果:模型组在休克1h后SOD活性明显低于对照组(P＜0.01)、MDA水平明显高于对照组(P＜0.01)。四逆汤组复苏3h后肝组织SOD明显高于生理盐水组(P＜0.01)、MDA低于生理盐水组(P＜0.01)、NO水平明显高于生理盐水组(P＜0.01)。生理盐水组iNOS在肝细胞中染色阳性单位明显高于四逆汤组(P＜0.05)。生理盐水组促进iNOSmRNA的表达。四逆汤组eNOSmRNA的表达增强。结论:四逆汤通过清除氧自由基, 升高NO, 改善肝组织微循环, 减少诱导型iNOS表达的各种因素, 理论上减轻了NO与氧自由基生成的ONOO的细胞毒作用和血管的低反应性, 并对肝脏起到保护作用。     

电子鼻技术应用于白及及其近似饮片快速辨识的可行性分析

目的：探讨电子鼻技术应用于白及及其近似饮片快速辨识的可行性。方法：收集134批白及及其近似饮片（白及45批、天麻30批、玉竹30批、黄花白及29批）作为待测样品,使用PEN3型电子鼻采集样品嗅觉感官数据作为自变量X,基于2020年版《中华人民共和国药典》和地方标准的鉴别结果,以及各饮片高效液相色谱法（HPLC）指纹图谱和原始采购信息,获得辨识模型的标杆数据Y,分别采用主成分分析-判别分析（PCA-DA）、偏最小二乘法-判别分析（PLS-DA）、最小二乘法-支持向量机（LS-SVM）及K-最近邻（KNN）4种化学计量学方法建立45批白及与89批非白及的二分类辨识模型和上述4种饮片的四分类辨识模型Y=F(X)。结果：经留一法交互验证,在二分类辨识中,上述4种模型分类正判率分别为97.01%、97.01%、98.51%和97.01%;在四分类辨识中,这4种模型分类正判率分别为97.76%、89.55%、98.51%和97.01%。二分类和四分类辨识模型的最高正判率均可达到98.51%,且均以LS-SVM算法为最优,最优核函数分别选择径向基核函数和线性核函数。最优模型判别结果良好,没有未分类样...     

疏肝和胃降逆汤联合雷贝拉唑治疗肝胃不和证反流性食管炎患者的疗效

目的：探究疏肝和胃降逆汤加减联合雷贝拉唑治疗肝胃不和证反流性食管炎（RE）患者的临床疗效。方法：选取莆田市涵江区中医院2021年2月至2022年2月期间收治的100例肝胃不和证RE患者,随机分为对照组与观察组,各50例。对照组患者采用雷贝拉唑、莫沙必利治疗,观察组患者采用疏肝和胃降逆汤加减联合雷贝拉唑治疗。比较两组患者的临床疗效、中医证候积分、血清相关指标及6个月复发率。结果：观察组患者治疗总有效率为94.00%,高于对照组的76.00%,差异具有统计学意义（P <0.05）。治疗后观察组患者各项中医证候积分低于对照组,差异具有统计学意义（P <0.05）。治疗后观察组患者血清胃动素（MTL）、胃泌素（GAS）水平高于对照组,生长抑素（SS）水平低于对照组,差异具有统计学意义（P <0.05）。治疗后观察组患者血清白细胞介素–1β(IL–1β)、IL–2、瘦素（Leptin）、降钙素基因相关肽（CGRP）水平低于对照组,差异具有统计学意义（P <0.05）。随访6个月,观察组失访4例,对照组失访3例。观察组患者6个月复发率为2.13%,低于对照组的18.42%,...