Identifying dysfunctional miRNA-mRNA regulatory modules by inverse activation,cofunction, and high interconnection of target genes: A case study of glioblastoma

Background

Accumulating evidence demonstrates that complex diseases may arise from cooperative effects of multiple dysfunctional miRNAs. Thus, identifying abnormal functions cooperatively regulated by multiple miRNAs is useful for understanding the pathogenesis of complex diseases.

Methods

In this study, we proposed a multistep method to identify dysfunctional miRNA-mRNA regulatory modules (dMiMRMs) in a specific disease, in which a group of miRNAs cooperatively regulate a group of target genes involved in a specific function. We identified dysfunctional miRNAs, which were differentially expressed and inversely regulated most of their target genes, by integrating paired miRNA and mRNA expression profiles and miRNA target information. Then, we identified cooperative functional units, in each of which a pair of miRNAs cooperatively repressed function-enriched and highly interconnected target genes. Finally, the cooperative functional units were assembled into dMiMRMs.

Results

We applied our method to glioblastoma (GBM) and identified GBM-associated dMiMRMs at the population, subtype, and individual levels. We identified 5 common dMiMRMs using all GBM samples, 3 of which were associated with the prognosis in patients with GBM and were better predictors of prognosis than were miRNAs or mRNAs alone. By applying our approach to GBM subtypes, we found consistent dMiMRMs across GBM subtypes, and some subtype-specific dMiMRMs were observed. Furthermore, personalized dMiMRMs were identified, suggesting significant individual differences in different patients with GBM.

Conclusions

Our method provides the capability to identify miRNA-mediated dysfunctional mechanisms underlying complex diseases.     

Type A alternating Wenckebach periodicity in the re-entrant path of ventricular extrasystoles

A patient with ventricular extrasystoles is reported in whom Type A alternating Wenckebach periodicity in the re-entrant path of the extrasystoles is suggested for the first time. Namely, it appears that 2:1 exit block occurs at a proximal level in the re-entrant path and block of the Wenckebach form occurs at a distal level in the path. The presence of three-level block in the re-entrant path is also suggested in this patient.     

Nosocomial pseudoepidemics and pseudoinfections: an increasing problem.

The infection control practitioner often relies on microbiologic data in order to conduct nosocomial infection surveillance and control activities. On the basis of a review of the medical literature, false positive culture and stained smear results representing pseudoinfection are being reported with greater frequency. Documented cases and clusters of pseudoinfectons are reviewed, and the epidemiologic characteristics and methods for detection and prevention of this increasingly recognized problem are discussed.     

基于专家调查法的针刺治疗缺血性中风恢复期疗效评价体系研究

临床试验中,疗效指标的选取直接决定了试验的质量。一套完整的疗效评价体系能够较为全面客观的反映研究的各个方面,从而能够为循证医学提供高质量的依据。目前我国中医药临床试验领域疗效评价体系的建立还处于初步阶段,本研究采用专家调查法,在文献分析的基础上,建立了针刺治疗缺血性中风恢复期临床疗效评价体系,以期为后续研究提供一定的借鉴和指导。     

长三角区域信息化合作背景、思路与对策

区域信息一体化是区域经济一体化的基础。长三角地区应打破行政分割,深化合作,建立健全信息化区域合作机制,从战略和政策层面构筑优势互补、资源共享、技术携手、互惠互利的信息化发展环境。这既是长三角地区经济一体化发展的需要,也是有效参与全球经济竞争,抢占全球信息化制高点的战略选择。     

普高与空军青航校毕业生抗荷体质差异

目的 对比青少年航空学校学员与普通高中生招飞抗荷体质相关指标差异,探索青少年航空学校抗荷体质训练成果并为训练方案优化提供科学依据。方法 随机抽取参加2022年空军招飞医学选拔定选阶段的部分青航校高三学员与同年级普通高中生作为研究对象,青航校学员按照既定培养方案,在高中阶段开展专项抗荷体质训练,并通过问卷调查收集普通高中生体育锻炼情况,纳入身体尺寸、身体成分、肌肉力量等指标进行对照研究,采用SPSS.26进行统计学分析。结果 青航校学员身高、四肢长、平静胸围、体质量均显著高于普通高中生(P＜0.05),二者坐高无统计学差异。青航校学员SMI显著高于普通高中生(P＜0.05),但体脂率对比无显著差异(P＞0.05);青航校学员背力与腿力均值/峰值显著高于普通高中生(P＜0.05);SMI与抗荷力量呈显著正相关,体脂率与抗荷力量不存在显著相关。青航校学员与普通高中生SMI分布存在差异,青航校学员的高SMI比例更高、低SMI比例更低(P＜0.05),而青航校与普通高中生体脂率分布无统计学差异(P＞0.05);结论 青航校抗荷体质训练在抗荷相关肌肉力量提高方面成效显著,但是在体脂率控制方面仍存在改进空间,未来青航校抗荷体质训练需要兼顾肌肉训练与体脂控制。     

单病种协作防治体系在医疗质量管理与监控中的作用探讨

加强医疗质量管理与监控是提升医疗质量,合理利用医疗资源,产生良好社会效益的有效手段。本文通过对医疗质量管理与现状的分析,剖析单病种协作防治体系在医疗质量管理与监控中的作用,为医疗质量管理与监控提供实践和理论基础。     

胆囊炎并发胆结石保守疗法与护理配合

老年胆囊疾病患者多数为慢性结石性胆囊炎,该病临床表现无特异性,起病缓慢,大多由急性胆囊炎反复发作迁延而至。由于年岁所限患者多采取保守疗法。如消炎利胆片、舒胆通、等口服药,起到了缓解病情的作用,但难根治。我院内科在治疗上加用了中药制剂,起到了疏肝理气、利胆通腑的药物疗效。同时护理人员注意调节好患者情志,使患者气血调和、气体通畅,加上规律饮食,确保患者及早康复。     

HACCP在学校饮用水卫生管理中的应用

目的通过应用危害分析和临界控制点(HACCP)管理系统,寻找学校饮用水卫生监督管理的方法,提高师生饮水的安全性。方法通过随机整群抽样,对13所市属学校进行摸底调查并抽样监测,获得资料。生活饮用水采集水样74份,纯净水成品水采集64份,饮水机水嘴处采集水样104份,应用HACCP系统前后采样检验。结果实施HACCP前后生活饮用水及饮水机消毒前后比较,差异有非常显著意义(P<0.01)。学校饮用水的主要危害有生物性危害和化学性危害,临界控制点包括卫生硬件设施、卫生制度、水质消毒、纯净水成品水质量、饮水机消毒。结论将HACCP应用于学校饮用水的质量控制过程中,是确保学生饮水安全的有效措施。     

卫生援外人力资源开发合作:现状、问题与策略

卫生人力资源开发合作是我国卫生援外工作的重要组成部分。本文通过文献研究、知情人访谈和现场调查,对卫生人力资源开发合作的管理机制、主要形式及特点进行系统梳理,发现:短期来华培训是目前卫生人力资源开发合作的主要形式,但对能力提高效果有限;学位学历教育比重尚小,但将成为精英人才培养的重要渠道;援外医疗队带教培训与技术合作的作用日益凸显;"走出去"培训模式投入小、周期短,但社会影响力大。存在的主要问题有:缺少战略性合作规划,需求契合度、与其他援外形式的结合度有待提高,评估体系不完善等。通过对欠发达国家卫生人力现状及对卫生人才培养的需求分析,建议制定卫生人力资源开发合作规划,促进卫生人才培养与其他援助形式的有机结合,完善监督评价制度,促进中国医学学位学历及培训项目获得当地认可,加强对发展中国家医学教育机构的援助,以增强发展中国家发展的"造血"机能,实现"共同发展"。