五官超短波与SSP低频脉冲电刺激联合治疗颞下颌关节紊乱的疗效

 目的 探讨五官超短波与SSP低频脉冲电刺激联合治疗颞下颌关节紊乱的临床疗效。方法 60例颞下颌关节紊乱病患者随机分为观察组和对照组各30例,两组均进行五官超短波治疗。观察组另给予SSP低频脉冲电刺激治疗。治疗前后分别进行评估,观察比较两组疗效并进行统计学处理。结果 经过2个疗程的治疗,临床疗效两组治疗前后差异比较有统计学意义(P<0.05);治疗后两组疼痛评分都有所下降(P<0.05),观察组明显优于对照组(P<0.05)。结论 五官超短波治疗颞下颌关节紊乱病效果良好,同时结合SSP低频脉冲电刺激治疗可以显著提高疗效,值得临床应用。     

B*4440: a novel HLA-B allele identified by sequence-specific oligonucleotide hybridization and sequence-specific amplification

A novel human leukocyte antigen B (HLA-B) allele, B*4440, is described. The allele was identified in an adult stem cell donor of Caucasian origin. HLA-B*4440 most closely matches to B*4403 differing by a substitution of three nucleotides at codon 44, 45, and 50. Thus, low-resolution HLA typing using sequence-specific oligonucleotide hybridization or amplification using sequence-specific primers gave inconclusive results. DNA sequencing confirmed a variation of codons 44 and 45 (AGG AAG-->AGA GAG) and codon 50 (CCA-->CCG), resulting in an amino acid substitution Lys-->Glu at codon 45.     

HLA-A位点PCR-SSP基因分型和血清学分型的比较

目的：建立优化ＨＬＡ－Ａ位点ＰＣＲ－ＳＰ分型方法。方法：采用普通扩增仪和Ｅｐｐｅｎｄｏｒｆ管对细胞系ＤＮＡ和３７个健康正常人进行基因分型,血清学检测采用标准淋巴细胞毒试验方法。结果：发现引物浓度和浓度比、ＤＮＡ的纯度及酶的选用,是影响ＨＬＡ－Ａ位点ＰＣＲ－ＳＳＰ分型准确性的重要因素。该方法对３７个标本的基因分型结果与血清学结果相符合。结论：ＰＣＲ－ＳＳＰ方法具有简便准确的优点,可以作为ＨＬＡ－Ａ位     

Secreted Phosphoprotein 1 (SPP1) Contributes to Second-Generation EGFR Tyrosine Kinase Inhibitor Resistance in Non-Small Cell Lung Cancer

Second-generation irreversible epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI), afatinib, has been approved for treating EGFR mutant lung cancer patients, but the mechanism of acquired resistance to afatinib has not been well studied. In this study, we established afatinib acquired resistant cell lines. Gene array technology was used to screen changes in gene expression between afatinib-resistant lung cancer cells and parental cells. Our results showed that secreted phosphoprotein 1 (SPP1) was significantly increased in afatinib-resistant lung cancer cells. To study the effect of SPP1 on afatinib resistance, siSPP1 was used to knock down SSP1 in afatinib-resistant lung cancer cells. Then sensitivity to afatinib and invasive ability were studied. We found that knockdown of SPP1 increased sensitivity of lung cancer cells to afatinib and decrease the ability of invasion. Of clinical significance, we found that SSP1 was upregulated in lung cancer tissues compared with adjacent normal tissues, and low level of SSP1 was strongly associated with better overall survival. Our results suggest that SPP1 enhanced the second-generation EGFR TKI resistance in lung cancer, and inhibiting SPP1 might be a therapeutic target to overcome afatinib resistance.     

A novel HLA-DR13 allele (DRB11314) identified by single-strand conformation polymorphism analysis and confirmed by direct sequencing

A novel variant of the HLA-DR13 group is described. The new allele was found in a DR 10, 13 heterozygous patient of Turkish origin, two HLA genotypically identical children of the patient typed as DR11, 13, and one child typed as DR13, 13. DR13 subtyping of the patient was initially performed by SSCP analysis of PCR-amplified DNA, using the 11th IHWS primers DRBAMP-3 and DRBAMP-B. Due to an unusual SSCP banding pattern, the PCR product was subjected to solid-phase sequencing. The sequence of the new allele, DRB1^*1314, is different from that of DRB 1^*1307 by a single nucleotide substitution in codon 47, with T replacing consensus A. This results in a single amino acid change of tryptophan to phenylalanine in the first domain of the DRβ chain.     

DNA TYPING SYSTEM FOR HLA-A2 ALLELES BY POLYMERASE CHAIN REACTION WITH SEQUENCE-SPECIFIC PRIMERS

Objectivs. To establish a PCR-SSP method for discriminating as many HLA-A 02 alleles, which could easilybe introduced into a routine laboratory. Methods. In this study we typed HLA-A 02 polymorphisms by a sequence-specific primer (SSP) method,which involved round 1 and round 2 PCR reactions to detect 17 HLA-A 02 alleles (they are HLA-A 0201- 0217 alleles) covering exon 2 and exon 3. Results. We have fmmd that DNA sample concentration and purity were the most important variables in determin-ing the quality of the results. For identiffing correct band size, the size marker used was important. We noticed that different PCR machines pedormed differently. By this method, we detected 20 HLA-A 02 positive genomic DNA samples and found 4 kinds of HLA-A 02 alleles. They were HLA-A 0201, 0203, 0206 and 0210. Condusion. The HLA-A 02 PCR-SSP method was proven to be a reliable and easily applicable typing method. Our results suggest that the SSP described here provides an optimal HLA-A 02 typing technique that may be useful in selecting donor-recipient pairs in bone marrow transplantation between unrelated individuals.     

Differentiation of autologous ABO, RHD, RHCE, KEL, JK, and FY blood group genotypes by analysis of peripheral blood samples of patients who have recently received multiple transfusions

BACKGROUND: After multiple transfusions, the serologic typing of autologous blood group phenotypes is difficult, because of mixed RBC populations. The genotyping of ABO, Rh, Kell, Kidd, and Duffy systems could be used to determine autologous blood group antigen status. STUDY DESIGN AND METHODS: Blood samples from patients and donors were analyzed before and after 26 multiple-transfusion events. An average of 6.9 non-WBC-reduced RBC units with an average age of 5.9 days were administered per transfusion event. The average period of blood sampling after transfusions was 5.3 days. All samples were serologically phenotyped for ABO, Rh, Kell, Kidd, and Duffy. Pretransfusion, posttransfusion, and buccal samples from patients were genotyped for the corresponding alleles by a uniform PCR sequence-specific primer protocol that allowed their simultaneous determination within 3 hours. RESULTS: All posttransfusion samples exhibited mixed-cell populations of various blood group systems on serologic testing. Genotyping from peripheral blood produced results identical to the autologous blood group phenotypes, regardless of the amount of blood transfused or of the length of the sampling period after transfusion. CONCLUSION: A fast and reliable PCR-sequence-specific primer DNA genotyping assay for simultaneous determination of autologous ABO, Rh, Kell, Kidd, and Duffy blood groups can be performed on peripheral blood samples, even though the patients have recently received multiple transfusions.