全文获取类型
收费全文 | 5509篇 |
免费 | 213篇 |
国内免费 | 72篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 4篇 |
妇产科学 | 8篇 |
基础医学 | 463篇 |
口腔科学 | 64篇 |
临床医学 | 577篇 |
内科学 | 626篇 |
皮肤病学 | 2篇 |
神经病学 | 31篇 |
特种医学 | 320篇 |
外科学 | 2147篇 |
综合类 | 556篇 |
预防医学 | 119篇 |
眼科学 | 1篇 |
药学 | 328篇 |
中国医学 | 516篇 |
肿瘤学 | 31篇 |
出版年
2024年 | 11篇 |
2023年 | 151篇 |
2022年 | 314篇 |
2021年 | 312篇 |
2020年 | 256篇 |
2019年 | 302篇 |
2018年 | 293篇 |
2017年 | 189篇 |
2016年 | 174篇 |
2015年 | 184篇 |
2014年 | 511篇 |
2013年 | 445篇 |
2012年 | 291篇 |
2011年 | 325篇 |
2010年 | 244篇 |
2009年 | 279篇 |
2008年 | 223篇 |
2007年 | 207篇 |
2006年 | 164篇 |
2005年 | 141篇 |
2004年 | 141篇 |
2003年 | 107篇 |
2002年 | 75篇 |
2001年 | 61篇 |
2000年 | 52篇 |
1999年 | 42篇 |
1998年 | 40篇 |
1997年 | 34篇 |
1996年 | 26篇 |
1995年 | 48篇 |
1994年 | 22篇 |
1993年 | 19篇 |
1992年 | 16篇 |
1991年 | 10篇 |
1990年 | 14篇 |
1989年 | 11篇 |
1988年 | 7篇 |
1987年 | 8篇 |
1986年 | 7篇 |
1985年 | 12篇 |
1984年 | 8篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1972年 | 2篇 |
排序方式: 共有5794条查询结果,搜索用时 15 毫秒
71.
72.
兔骨关节炎软骨细胞中bcl-2、bax mRNA及蛋白表达的意义 总被引:1,自引:0,他引:1
目的探讨bcl-2、bax基因在兔骨关节炎软骨细胞凋亡中的意义。方法新西兰白兔12只,随机分为模型组,按照石膏外固定兔后膝关节造模,以及空白对照组,6周后处死动物取得膝关节软骨。采用免疫组织化学和原位杂交检测bcl-2、bax mRNA及蛋白表达。结果模型组软骨细胞bcl-2、bax mRNA表达明显多于对照组(P<0.05)。免疫组织化学模型组软骨细胞bcl-2、bax阳性表达灰度值明显降低,与对照组比较差异有统计学意义(P<0.05);阳性细胞比例明显提高,与对照组比较差异有统计学意义(P<0.01)。模型组bcl-2/bax阳性细胞率比值比对照组低,差异有统计学意义(P<0.05)。结论bcl-2和bax共同参与了兔骨关节炎模型软骨细胞凋亡的调节,结果导致软骨细胞凋亡加快。 相似文献
73.
伸膝制动骨关节炎动物模型软骨内胶原变化的观察 总被引:12,自引:1,他引:12
目的 对骨关节炎(OA)早期软骨内胶原变化进行动态观察,探时胶原变化与OA发病机制的关系。方法 采用兔膝关节伸直位制动OA模型,对制动10、20、30、40d的关节软骨进行透射电镜观察,Ⅱ型胶原原位杂交,I、Ⅱ、Ⅲ型胶原的免疫组织化学测定。结果 电镜观察显示OA关节软骨的破坏从切线层的细胶原纤维网开始,而切线层的细胶原纤维网不含细胞;原位杂交和免疫组织化学显示OA早期Ⅱ型胶原的表达和含量增加,而且增加主要发生在移行层和深层上部,二者染色灰度值随时间的变化是先递增,之后逐渐减少;OA关节软骨内有Ⅲ型胶原分泌,并呈增加趋势,但无I型胶原的分泌。结论 OA关节软骨的退变从切线层开始,切线层胶原破坏后难于修复可能是软骨退变重要原因。 相似文献
74.
BACKGROUND Osteoarthritis is a major source of pain,disability,and socioeconomic cost worldwide.Osteonecrosis is a disabling disorder that frequently occurs in the younger population aged from 20-50 years.The compound Eucommia bone tonic granules,a traditional Chinese medicine,can alleviate the damage of osteoarthritis and osteonecrosis.AIM To investigate the potential role of the compound Eucommia bone tonic granules(Eucommia)in the treatment of patients with osteoarthritis and osteonecrosis.METHODS One-hundred forty osteoarthritis and osteonecrosis cases admitted to our hospital from January 2013 to December 2017 were selected.Patients were divided into two groups:Eucommia-meloxicam group and meloxicam group.Clinical efficacy and the Western Ontario and McMaster Universities Arthritis Index(WOMAC)score were evaluated according to the evaluation criteria of orthopedic diseases.The levels of bone-GLA protein,interleukin-17,recombinant human S100 calcium binding protein A12,sphingosine 1-phosphate,cystatin C,creatinine,and hemoglobin in peripheral blood were determined.RESULTS The total effective rate in the two osteoarthritis groups was not different,but the total effective rate in the two osteonecrosis groups was significantly different.The overall efficacy of Eucommia-meloxicam group was superior to that of the meloxicam group.WOMAC showed that pain,stiffness,and dysfunction in the two groups of osteoarthritis and osteonecrosis before and after treatment were significantly different.The concentration of recombinant human S100 calcium binding protein A12,sphingosine 1-phosphate,cystatin C,creatinine,and hemoglobin before and after treatment in the Eucommia-meloxicam group and meloxicam group of osteoarthritis and osteonecrosis were significantly different,and the two treatment groups were significantly different from each other for osteoarthritis.CONCLUSION Our findings indicate that Eucommia can effectively enhance the curative effect of meloxicam,and the combination of Eucommia and meloxicam is superior to meloxicam alone. 相似文献
75.
Katherine A. Glass Jarrett M. Link Jonathan M. Brunger Franklin T. Moutos Charles A. Gersbach Farshid Guilak 《Biomaterials》2014
The pathogenesis of osteoarthritis is mediated in part by inflammatory cytokines including interleukin-1 (IL-1), which promote degradation of articular cartilage and prevent human mesenchymal stem cell (MSC) chondrogenesis. In this study, we combined gene therapy and functional tissue engineering to develop engineered cartilage with immunomodulatory properties that allow chondrogenesis in the presence of pathologic levels of IL-1 by inducing overexpression of IL-1 receptor antagonist (IL-1Ra) in MSCs via scaffold-mediated lentiviral gene delivery. A doxycycline-inducible vector was used to transduce MSCs in monolayer or within 3D woven PCL scaffolds to enable tunable IL-1Ra production. In the presence of IL-1, IL-1Ra-expressing engineered cartilage produced cartilage-specific extracellular matrix, while resisting IL-1-induced upregulation of matrix metalloproteinases and maintaining mechanical properties similar to native articular cartilage. The ability of functional engineered cartilage to deliver tunable anti-inflammatory cytokines to the joint may enhance the long-term success of therapies for cartilage injuries or osteoarthritis. 相似文献
76.
Chondroitin sulfate and glucosamine sulfate exert beneficial effects on the metabolism of in vitro models of cells derived from synovial joints: chondrocytes, synoviocytes and cells from subchondral bone, all of which are involved in osteoarthritis (OA). They increase type II collagen and proteoglycan synthesis in human articular chondrocytes and are able to reduce the production of some pro-inflammatory mediators and proteases, to reduce the cellular death process, and improve the anabolic/catabolic balance of the extracellular cartilage matrix (ECM). Clinical trials have reported a beneficial effect of chondroitin sulfate and glucosamine sulfate on pain and function. The structure-modifying effects of these compounds have been reported and analyzed in recent meta-analyses. The results for knee OA demonstrate a small but significant reduction in the rate of joint space narrowing. Chondroitin sulfate and glucosamine sulphate are recommended by several guidelines from international societies for the management of knee and hip OA, while others do not recommend these products or recommend only under condition. This comprehensive review clarifies the role of these compounds in the therapeutic arsenal for patients with knee OA. 相似文献
77.
Functional and phenotypical analysis of IL‐6‐secreting CD4+ T cells in human adipose tissue 下载免费PDF全文
Anja J. de Jong Sabrina Pollastro Joanneke C. Kwekkeboom Stefan N. Andersen Annemarie L. Dorjée Aleida M. Bakker Fawaz Alzaid Antoine Soprani Rob G.H.H. Nelissen Jan B. Mullers Nicolas Venteclef Niek de Vries Margreet Kloppenburg René E.M. Toes Andreea Ioan‐Facsinay 《European journal of immunology》2018,48(3):471-481
78.
Background
There are unanswered questions about knee–ankle alignment after total knee arthroplasty (TKA) for varus and valgus osteoarthritis (OA) of the knee. The aim of this retrospective study was to assess knee–ankle alignment after TKA.Methods
The study consisted of 149 patients who had undergone TKA due to varus and valgus knee OA. The alignment and angles in the selected knees and ankles were measured on full-length standing anteroposterior radiographs, both pre-operatively and post-operatively. The paired t-test and Pearson's correlation tests were used for statistical analysis.Results
The results showed that ankle alignment correlated with knee alignment both pre-operatively and postoperatively (P < 0.05). The pre-operative malalignment of the knee was corrected (P < 0.05), and the ankle tilt angle was accordingly improved in the operative side after TKA (P < 0.05). In addition, TKA had little effect on knee–ankle alignment on the non-operative side (P > 0.05).Conclusion
These findings indicated that routine TKA could correct the varus or valgus deformity of a knee, and improve the tilt of the ankle. Ankle alignment correlated with knee alignment both pre-operatively and postoperatively. Both pre-operative knee and ankle malalignment can be simultaneously corrected following TKA.Level of evidence
Level III. 相似文献79.
《The Knee》2021
BackgroundThe cruciate retaining lipped (CR-lipped) bearing is designed to provide more anterior-posterior (AP) stability and could be employed to resolve excessive intraoperative laxity during the cruciate retaining TKA (CR-TKA). The aim of the study was to determine whether the CR-lipped bearing in CR-TKAs with a perioperative excessive laxity allows equivalent functional results as compared to the standard CR articulation.MethodsA cohort of 111 TKAs with CR-lipped bearings was matched to a cohort of conventional CR bearings regarding age and sex. The CR-lipped bearing was used in patients with excessive knee AP laxity and the regular CR bearing was used in patients without excessive AP laxity during TKA. Various PROMs (WOMAC, KSS, SF-36) were assessed preoperatively and at 5-years postoperative in combination with revision rate and Range of Motion (ROM).ResultsPROMs did not differ significantly between both groups 5-years postoperatively. Mean ROM (flexion) 5-years postoperatively was not significantly different. The implant survivorship was 100% for both cohorts with revision for any reason as end point.ConclusionBased on these results, the CR-lipped bearing is a safe and effective solution for mild interoperatively assessed PCL laxity during CR-TKA without loss of function or decreased survivorship at 5 years. Peroperative conversion to a PS-TKA in order to obtain satisfactory functional scores might therefore not be necessary when mild PCL laxity is observed during surgery. Further research should focus on verifying this approach and longer follow-up is needed to generate data on long term survivorship.Level of evidenceLevel IV therapeutic, retrospective, cohort study. 相似文献
80.
《The Knee》2022
BackgroundIn recent years, transcatheter arterial embolization (TAE) using imipenem/cilastatin (IPM/CS) has attracted attention as a treatment for relieving osteoarthritis (OA) pain. However, IPM/CS is not approved by Japanese medical insurance for use as an embolic material. Therefore, it is necessary to develop new embolic materials for TAE to relieve OA pain. The purpose of this study was to develop a swine model of knee arthritis and embolize abnormal neovessels (ANs) using two different embolic materials. We compared the embolic effects and tissue damage in knees.MethodsKnee arthritis was induced by intra-articular injection of papain into 12 knees in six female swine. The swine were divided into two groups of three swine each (six knees per group) for embolization of ANs in the knees with either IPM/CS or soluble gelatin sponge particles (SGSs). Three days after embolization, we compared the embolic effects using angiography and the tissue damage histopathologically.ResultsANs were observed in all 12 knees at 42 days after papain injection. The ANs disappeared and the patent arteries were recanalized 3 days after TAE in all 12 knees. Histopathological evaluation revealed synovitis changes, such as synovial thickening and inflammatory cell infiltration, in all 12 knees. There was no evidence of skin or muscle necrosis in either group. The appearance of ANs, recanalization of the parent arteries, and histopathological outcomes were not significantly different between the two groups.ConclusionSGSs were as safe as IPM/CS for TAE of ANs in this swine model of knee arthritis. 相似文献