Comparative study on radiosensitivity of various tumor cells and human normal liver cells

AIM: To investigate the radiation response of various human tumor cells and normal liver cells. METHODS: Cell lines of human hepatoma cells (SMMC-7721), liver cells (L02), melanoma cells (A375) and cervical tumor (HeLa) were irradiated with 60Co γ-rays. Cell survive was documented by a colony assay. Chromatid breaks were measured by counting the number of chromatid breaks and isochromatid breaks immediately after prematurely chromosome condensed by Calyculin-A. RESULTS: Linear quadratic survival curve was observed in all of four cell lines, and dose-dependent increase in radiation-induced chromatid and isochromatid breaks were observed in GB2B phase. Among these four cell lines, A375 was most sensitive to radiation, while, L02 had the lowest radiosensitivity. For normal liver cells, chromatid breaks were easy to be repaired, isochromatid breaks were difficult to be repaired. CONCLUSION: The results suggest that the y-rays induced chromatid breaks can be possibly used as a good predictor of radiosensitivity, also, unrejoined isochromatid breaks probably tightly related with cell cancerization.     

Cx26基因在正常肝脏和肝癌中的表达

间隙连接蛋白(Cx)基因在胚胎发育、细胞生长、分化及细胞间信号传递、物质交流等方面起着重要作用,Cx基因的表达及功能异常与肿瘤发生密切相关.肿瘤细胞常存在Cx基因表达下调或缺失.肝癌是最常见的恶性肿瘤之一.连接蛋白26是哺乳动物肝脏中的主要表达的连接蛋白之一,近年来研究表明它对肝脏GJIC有重要作用.Cx26蛋白的表达异常可能是肝细胞癌发生重要机制之一.     

Clinical significance of red blood cell distribution width in the elderly: a potential indicator of bone marrow stimulation by erythropoietin

Summary To investigate the clinical significance of red blood cell distribution width (RDW) and haemoglobin distribution width (HDW) in the elderly and their relationships with erythropoietin (EPO) secretion, we measured red cells parameters using a Technicon HI system and serum EPO using a radioimmunoassay in 247 elderly subjects (normal: n= 150; preanaemic iron deficiency: n= 24; iron deficiency anaemia: n= 8; senile anaemia: n= 65). RDW was slightly higher in the elderly subjects with preanaemic iron deficiency (14.1 ± 1.1%) than in the normal elderly subjects (13.5±0.7%). It was highest in iron deficiency anaemia (16.1 ± 1.3%), while the increase in senile anaemia was limited (13.9 ± 1.2%). The HDW increased only in iron deficiency anaemia. There was a strong positive relationship between EPO and RDW in iron deficiency anaemia (r= 0.817, P<0.01). Moreover, this correlation was also found in preanaemic iron deficiency (r= 0.456, P < 0.05), but not in senile anaemia, suggesting that bone marrow hypoactivity may partly play a role in the pathogenesis of senile anaemia. All the eight subjects with iron deficiency anaemia had a RDW ≥ 14.9% (mean + 2SD of normal subjects), while 55 (85%) of the 65 with senile anaemia had a RDW < 14.9%. Both the RDW and EPO levels of six anaemic subjects with high RDW values (≥ 14.9%) after oral iron therapy for 56–78 days decreased significantly. Our results suggest that RDW is useful to distinguish iron deficiency anaemia from senile anaemia, and may be a potential parameter of bone marrow stimulation by EPO.     

江苏省6～15岁学生甲状腺容积正常值调查

目的建立江苏省6￣15岁儿童甲状腺容积正常值范围参考值。方法在江苏省用B超法对4468例碘营养状况正常的6￣15岁学生进行甲状腺容积及体表面积测量。结果6￣15岁学生甲状腺容积上限、中位数和下限值均随年龄而升高,但在10岁以后其升高速度明显变缓。性别对甲状腺容积无影响。根据体表面积计算甲状腺容积时,不同性别上限、中位数和下限数值十分相似和接近。体表面积计算甲状腺容积比用年龄计算更为实用。结论确定了江苏省6～15岁碘营养正常儿童各年龄组甲状腺容积正常值范围,该正常值范围的确定对全省碘缺乏病监测及全国修订儿童甲状腺容积正常值具有一定的参考价值。     

Insulin requirement of simple and complex carbohydrate foods in type 1 (insulin-dependent) CSII-treated diabetic subjects,obtained by Biostator. Correlation with glycaemic index

Summary The aim of this work was to observe whether different types of carbohydrates might require different insulin doses. Five type 1 CSII-treated diabetic subjects (age 39±4 years), C-peptide negative and in optimal metabolic control (HbA_1c 7.5±0.2) were selected for the study. They were connected to the Biostator 6 times with an interval of 4–10 days between sessions and fed a meal containing 75 g of carbohydrates of different types: bread, pasta, potatoes, apples, oranges and sucrose. The following net (above basal) insulin requirement for the 30 meals were found (IU-mean+SD): bread 9.15±1.97; pasta 6.00±1.37; potatoes 7.05±2.58; apples 4.54±1.42; oranges 6.21±2.62; sucrose 7.83±2.33. A statistically significant difference was found by ANOVA among insulin requirements for foods (p<0.05). Single comparisons between bread and the other foods showed a statistically significant difference only between bread and apple (p<0.05). Mean coefficient of variation was 33.9% for the subjects and 30.7% for the meals. A significant correlation was found between Jenkins’ glycaemic index and insulin requirement (r=0.897; p<0.001). In conclusion, the high intraindividual variability of insulin requirement does not advice the use of the glycaemic index during optimized insulin therapy.     

Effect of losartan on angiotensin II-mediated endothelin and prostanoid excretion in humans

Angiotensin II (Ang II) stimulates renal prostanoid and vascular endothelin-1 (ET-1) release. Most known Ang II effects are mediated by AT₁ receptors. Our aim was to determine whether AT₁ receptor activation mediates Ang II-evoked renal prostanoid and ET-1 release. Eleven healthy men were randomized in a crossover, double-blind fashion to receive 100 mg/day of losartan or matching placebo, for 8 days. Blood and urine were sampled before and after a 2-h infusion of Ang II at a rate previously determined to increase mean arterial pressure (MAP) by 25 to 30 mm Hg in each subject. After a 14-day washout, subjects received the alternate treatment. Pretreatment with losartan had little effect on baseline MAP, but increased plasma renin activity, and virtually eliminated the pressor response to Ang II infusion. Angiotensin II significantly increased prostanoid excretion after placebo; the prostanoid response to Ang II was even greater after losartan. Plasma ET-1 was not altered by Ang II infusion, with or without losartan. In contrast, urine ET-1 excretion rate decreased to 40% of baseline after Ang II but not after losartan pretreatment; losartan alone had no effect. We conclude that Ang II decreases renal ET-1 synthesis and release through the AT₁ receptor. In contrast, Angiotensin II-mediated renal prostanoid synthesis does not require activation of AT₁ receptors. These findings indicate that AT₁ receptor antagonists could provide renal protection through indirect mechanisms.     

Immunologisch meßbares Insulin (IMI) bei Stoffwechselgesunden,Fettsüchtigen und adipösen Diabetikern nach intravenöser Gabe von Glucose,Tolbutamid und Glucagon

Zusammenfassung Bei Stoffwechselgesunden, Übergewichtigen und übergewichtigen Altersdiabetikern wurde das immunologisch meßbare Insulin (IMI) nach intravenöser Glucose (0.33 g/kg), Tolbutamid (1 g) und Glucagonbelastung (1 mg) bestimmt. —Bei Stoffwechsel-gesunden steigen die IMI-Werte schon in den ersten Minuten nach der Glucose-, Tolbutamid-bzw. Glucagonbelastung an. Von der 15. Minute ab liegen die IMI-Werte wieder im Bereich der Norm. Die Übergewichtigen reagieren auf die Glucose-, Tolbutamid-und Glucagon-Stimulation so prompt wie die Normalgewichtigen; der Anstieg erfolgt in den ersten Minuten, die Werte liegen jedoch signifikant höher. Bei Übergewichtigen mit manifestem Diabetes liegen die IMI-Werte nach Glucose-und Tolbutamidbelastung wesentlich niedriger, nach Glucagon jedoch im Bereich der Stoffwechselgesunden mit normalem Körpergewicht. —Zwischen dem Koeffizienten K der Glucoseassimilation und dem Seruminsulin nach der Glucosebelastung konnte bei Übergewichtigen keine Korrelation gefunden werden. Damit ist der Hyper-insulinismus mehr als Charakteristikum der Fettsucht als des Diabetes mellitus anzusehen.

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Immunologically measurable insulin (IMI) in normal, obese and obese, diabetic subjects after intravenous administration of glucose, tolbutamide and glucagon.

Summary Serum insulin levels (immunologically measurable insulin, IMI) in normal, obese and obese, diabetic subjects were determined following injection of glucose (0.33 g/kg), tolbutamide (1 g) and glucagon (1 mg). —In normal non-obese subjects an immediate (after one minute) increase of serum insulin levels was observed following the injection of glucose, tolbutamide or glucagon. 15 minutes after injection, the IMI-values were within the normal range. Obese subjects also react with an immediate increase of serum insulin levels following injection of glucose, tolbutamide or glucagon; however, the IMI-values were significantly higher than in normal subjects. Furthermore, in obese patients with slight diabetes, the IMI-levels after glucose or tolbutamide were definitely lower than those observed in normal non-obese subjects, although the levels following glucagon were of the same magnitude.-Apparently the increase of endogenous insulin in obese non-diabetic-patients after administration of glucose does not influence the glucose assimilation coefficient, K. Hyperinsulinism appears to be associated with obesity rather than with diabetes.

Durchgeführt mit Unterstützung der Deutschen Forschungsgemeinschaft, Bad Godesberg.     

Efficacy and safety of once-weekly semaglutide 1.0 mg vs once-daily liraglutide 1.2 mg as add-on to 1–3 oral antidiabetic drugs in subjects with type 2 diabetes (SUSTAIN 10)

AimsSUSTAIN 10 compared the efficacy and safety of the anticipated most frequent semaglutide dose (1.0 mg) with the current most frequently prescribed liraglutide dose in Europe (1.2 mg), reflecting clinical practice.MethodsIn this phase 3b, open-label trial, 577 adults with type 2 diabetes (HbA_1c 7.0–11.0%) on 1–3 oral antidiabetic drugs were randomized 1:1 to subcutaneous once-weekly semaglutide 1.0 mg or subcutaneous once-daily liraglutide 1.2 mg. Primary and confirmatory secondary endpoints were changes in HbA_1c and body weight from baseline to week 30, respectively.ResultsMean HbA_1c (baseline 8.2%) decreased by 1.7% with semaglutide and 1.0% with liraglutide (estimated treatment difference [ETD] –0.69%; 95% confidence interval [CI] -0.82 to -0.56, P < 0.0001). Mean body weight (baseline 96.9 kg) decreased by 5.8 kg with semaglutide and 1.9 kg with liraglutide (ETD -3.83 kg; 95% CI -4.57 to -3.09, P < 0.0001). The proportions of subjects achieving glycaemic targets of < 7.0% and = 6.5%, weight loss of = 5% and = 10%, and a composite endpoint of HbA_1c < 7.0% without severe or blood glucose-confirmed symptomatic hypoglycaemia and no weight gain were greater with semaglutide vs liraglutide (all P < 0.0001). Both treatments had similar safety profiles, except for more frequent gastrointestinal disorders (the most common adverse events [AEs]) and AEs leading to premature treatment discontinuation with semaglutide vs liraglutide (43.9% vs 38.3% and 11.4% vs 6.6%, respectively).ConclusionSemaglutide was superior to liraglutide in reducing HbA_1c and body weight. Safety profiles were generally similar, except for higher rates of gastrointestinal AEs with semaglutide vs liraglutide.     

长春地区健康成人心肌19种元素的正常值范围

由吉林省长春地区采集40例健康成人心肌样品,用荧光分光光度法测定硒,用催化极谱法测定钼,用高频感耦等离子体发射光谱法测定铝、硼、钡、钙、镉、钴、铬、铜、铁、钾、镁、锰、钠、磷、锶、钛和锌的含量。取90%可信限,通过计算确定了健康成人心肌19种元素的正常值范围。     

Effects of Age, Gender, Bolus Volume, and Trial on Swallowing Apnea Duration and Swallow/Respiratory Phase Relationships of Normal Adults

The effects of age, gender, bolus volume, and trial on swallowing apnea duration (SAD) and swallow/respiratory phase relationships were examined. Sixty adults, composed of ten males and ten females in each of three age groups (i.e., 20–39, 40–59, and 60–83 years), participated. SAD was assessed via nasal airflow during saliva swallows and 10-, 15-, 20-, 25-mL bolus volumes across three trials. Results revealed SAD is consistent across trial (p>0.05). Significant main effects of age, gender, and bolus volume were found (p<0.05), i.e., elderly adults had longer SAD than young and middle-aged adults; women had longer SAD than men; and SAD increased as bolus volume increased. With respect to saliva swallows, a significant interaction of age by gender was found (p<0.05), i.e., males exhibited a decrease in SAD with increasing age while females exhibited an increase in SAD with increasing age. Concerning swallow/respiratory phase relationships, the pattern of exhale–swallow–exhale was evident during 62% of participants' swallows. Furthermore, age, gender, or bolus volume did not predict the pattern of exhale–swallow–exhale (p>0.05). Submitted February 23, 2000; accepted October 2, 2000