Experimental study of subcutaneous heterotopic intestinal graft — effect of prostaglandin E1 on the viability

Summary In this study, prostaglandin E₁ (PGE₁) was administered daily in a subcutaneous heterotopic jejunal model in rats to compare the results of the viability rate to those in the non-PGE, group. The viability rate of the PGE₁ group was 28.6% in the day 5 ligated group, 75.0% in the day 7 ligated group and 100% in the day 14 ligated group. The viability rate of the non-PGE₁ group was 0%, 37.5%, and 100%. The results of the PGE₁ group in the day 5 and day 7 ligated groups were significantly different from those in the non-PGE₁ group. These results indicate that the administration of PGE₁ facilitates the neovascularization necessary for survival of the grafted tissue and shortens the time for the graft to become viable at the implantation site.     

肺癌MRI动态增强模式与肿瘤血管生成的相关性研究

目的　探讨肺癌MRI动态增强模式与肿瘤血管生成的关系。方法　对 4 8例手术、病理证实的肺癌于术前行动态增强MR扫描 ,绘制时间 信号强度曲线并计算动态增强MRI参数 ,如最大增强线性斜率 (steepestslope ,SS)、增强峰值 (peakheight,PH)、增强后第 1、2、4分钟时信号强度改变率(E1、E2 、E4)等 ,分析各动态增强MRI参数及强化形态学表现 ,并与肺癌的微血管密度 (microvasculardensity,MVD)作相关性分析。结果　 4 8例肺癌微血管密度均数为 (5 6 .0 7± 2 2 .6 1)条 /0 .74mm2 。其中鳞癌 (2 6例 )为 (4 4 .4 1± 16 .2 8)条 /0 .74mm2 ,腺癌 (2 2例 )为 (6 9.86± 2 1.4 7)条 /0 .74mm2 条 /0 .74mm2 ,MVD计数腺癌高于鳞癌 ,两者间差异有显著意义 (t =4 .6 6 6 ,P <0 .0 1)。动态增强MRI各参数SS、PH、E1、E2 、E4与肺癌MVD呈正相关 ,其中以SS与MVD相关性最强 (r=0 874 ,P <0 .0 1)。结论　肺癌的微血管密度是其在动态增强MRI上不同表现的病理生理学基础 ,肺癌MRI动态增强模式与肿瘤血管生成有良好的相关性。     

Differential expression of tenascin-C and tenascin-X in human astrocytomas

Tenascins (TNs) are a family of extracellular matrix glycoproteins. The first member of this family to be recognized, tenascin-C (TN-C), is known to be expressed in various tumors including human astrocytomas. Tenascin-X (TN-X) is the latest member of the TN family to be reported, and its expression in tumor tissues has not yet been examined. In this study, we found expression of TN-X in glioma cell lines and human astrocytomas by immunoblot analysis using anti-mouse TN-X antibodies. We also examined the expression of TN-C and TN-X immunohistochemically in a series of 32 human astrocytomas and tissue from 5 normal brains. Expression of TN-X was up-regulated to a higher degree in low-grade astrocytomas than in high-grade astrocytomas. TN-X was mainly localized in the perivascular stroma around tumor vessels, and weakly expressed in the intercellular spaces among tumor cells. In contrast, TN-C was more strongly expressed in the intercellular spaces and in tumor vessels in high-grade astrocytomas (anaplastic astrocytomas and glioblastomas) than in low-grade astrocytomas. In the tissues expressing both TNs, the distribution of TN-X was often reciprocal to that of TN-C. These findings indicate that the expression of TN-C and TN-X in astrocytomas is different, and that these glycoproteins could be involved in neovascularization in different manners. Received: 7 June 1996 / Revised: 25 October 1996 / Accepted: 30 October 1996     

血管生成抑制剂TNP-470抑制血管瘤血管内皮细胞增殖的体外实验研究

目的 观察血管生成抑制剂TNP-470对血管瘤血管内皮细胞体外增殖的影响，并与氢化可的松相比较。方法 选择一草莓状血管瘤，采用M199培养液按组织块法进行血管内皮细胞(VEC)体外培养。传至第3代，VEC分为6组：组1、2、3在培养液中加入内皮细胞生长支持物(ECGS)，组1为对照组，组2加入氢化可的松，组3加入TNP-470；组4、5、6培养液中加入ECGS和雌二醇(E2)，组4为对照组，组5加入氢化可的松，组6加入TNP-470。培养后3、6、9d分别行细胞计数和3H-TdR掺入检测。结果 至第9d，组1VEC增殖明显，约增至3倍。组2、组3VEC增殖受到抑制，仅为组1的1/10(均P＜0．01)。组4VEC增殖最为显著，约为组1的2倍。组5、组6VEC增殖仍受到明显抑制，约为组4的1／7(均P＜0．01)。组2与组3、组5与组6VEC增殖状况相似(均P＞0．05)。结论 TNP-470能明显抑制ECGS或ECGS和E2联合诱导的血管瘤VEC体外增殖，抑制作用与氢化可的松程度相仿。     

脉络膜新生血管患者房水中血管内皮生长因子和色素上皮衍生因子的浓度

目的:研究血管内皮生长因子(VEGF)和色素上皮衍生因子(PEDF)在活动性脉络膜新生血管膜(CNV)患者房水中的表达。方法:应用酶联免疫吸附测定法对32例活动性CNV患者和10例白内障患者(对照组)的房水标本进行VEGF和PEDF检测。结果:CNV患者房水中的VEGF为(523.0±273.7)pg/m l,PEDF为(16.58.±13.11)ng/m l;对照组患者房水中的VEGF为(108.3±72.3)pg/m l,PEDF为(0.35±0.57)ng/m l。两组间的VEGF和PEDF均有显著性差异(均P=0.000)。结论:活动性CNV患者房水中VEGF和PEDF增高,可能与脉络膜新生血管的形成有关。     

血管化对聚酯/钙磷盐支架修复兔桡骨缺损及支架降解的影响

目的:探索应用生长因子促进骨移植材料血管化的方法,检验血管化对新骨形成及移植物降解等方面的影响。方法:分别采用复合bBM P+bFGF及单纯复合bBM P的聚酯/钙磷盐载体材料(PLGA/TCP)修复兔桡骨15mm缺损,通过量化评价、影像学、组织学、材料降解及骨密度评价血管化对聚酯/钙磷盐载体材料修复兔桡骨缺损效果的影响。结果:术后12W时各组骨缺损均有不同程度的愈合,但复合bBM P+bFGF组与单纯复合bBM P组相比较,各检测指标均有显著性差异,加入血管化因素bFGF可明显提高骨缺损部位的血管化程度,加快骨缺损修复速度及材料降解速度。结论:加入bFGF作为血管化影响因素后,用PLGA/TCP材料修复兔桡骨缺损,骨缺损处的血管化程度有一定程度的提高,并相应的加速了骨修复速度及材料降解速度。     

Progranulin (granulin-epithelin precursor,PC-cell-derived growth factor,acrogranin) mediates tissue repair and tumorigenesis

Progranulin (Pgrn) is a pluripotent secreted growth factor that mediates cell cycle progression and cell motility. It activates the extracellular regulated kinases and phosphatidyl inositol-3 kinase signal cascades, among others, and increases expression of cyclins D and B. Structurally, it belongs to none of the well-established growth factor families. It regulates developmental events as diverse as the onset of cavitation in the preimplantation embryo and male-specific brain differentiation. During wound repair it promotes granulation and neovascularization. It regulates inflammation through a tripartite loop with secretory leukocyte protease inhibitor (SLPI) which protects pgrn from proteolysis, and elastase, which digests it to smaller peptides. Intact pgrn is anti-inflammatory through the inhibition of some of the actions of tumor necrosis factor, while the proteolytic peptides may stimulate the production of proinflammatory cytokines such as interleukin 8. Pgrn is highly expressed in aggressive cancer cell lines and clinical specimens including breast, ovarian, and renal cancers as well as gliomas. In experimental systems it confers an aggressive phenotype on poorly tumorigenic epithelial cancer cells. The malignancy of highly tumorigenic progranulin-expressing cell lines depends on the expression level of the pgrn gene since attenuating pgrn mRNA levels in pgrn-responsive cells greatly inhibits tumor progression. Given its actions in wound repair and tumorigenesis pgrn may prove a useful clinical target, both for prognosis and for therapy.Abbreviations EGF Epidermal growth factor - FAK Focal adhesion kinase - FGF Fibroblast growth factor - grn/epi Granulin-epithelin - IGF Insulin-like growth factor - MAPK Mitogen-activated protein kinase - MEF Mouse embryo fibroblast - PDGF Platelet-derived growth factor - Pgrn Progranulin - PI-3K Phosphatidylinositol-3 kinase - SLPI Secretory leukocyte protease inhibitor - TGF- Transforming growth factor- - TGFe Epithelial transforming growth factor - TNF Tumor necrosis factor - VEGF Vascular endothelial growth factor     

血管内皮祖细胞与缺血性心血管病的血管新生

1997年Ｔａｋａｙｕｋｉ等[1] 在Ｓｃｉｅｎｃｅ杂志发表论文报道血管内皮祖细胞 (ｅｎｄｏｔｈｅｌｉａｌｐｒｏｇｅｎｉｔｏｒｃｅｌｌｓ ,ＥＰＣ)分离成功 ,并可用于体内血管新生 (ａｎｇｉｏｇｅｎｅｓｉｓ)。当时提出的血管内皮祖细胞认为是假定的 (ｐｕｔａｔｉｖｅ)。但是 ,近年来随着干细胞研究的深入 ,血管内皮祖细胞的存在基本已经确认 ,而且它与血管新生间的关系日益受到重视[2 ,3 ] 。本文将讨论血管内皮祖细胞的特征 ,它与缺血性心血管疾病中血管新生的关系及其可能的应用前景。1　血管内皮祖细胞的特征　　血管内皮祖细胞是血…     

AN IMMUNOHISTOCHEMICAL STUDY OF NEOVASCULATURE IN HUMAN BRAIN TUMORS

In order to elucidate the characteristics of newly-formed small blood vessels in brain tumors, 51 cases of various types of brain tumor were studied immuno-histochemically. Endothelial cells in all tumor types reacted positively with anti-Factor VIH-related antigen (F-VIII Ag) and UEA-1. Reactivity to UEA-1 was limited to endothelial cytoplasm. Proliferating immature endothelial cells showed weak reactivity to anti-F-VIII Ag. In some cases of benign tumors diffuse reactivity to anti-F-VIII Ag was noted throughout thickened medial areas of the vascular walls, in which reactivity to anti-fibrous collagen and anti-flbronectin was noted. UEA-1 was thought to be a more specific endothelial marker than F-VIII Ag, especially for proliferating and Immature endothelial cells. It is strongly suspected that fibrous collagens are related to the formation of a thickened vascular wall in newly-formed small tumor vessels.     

口服大剂量MPA对子宫内膜癌血管生成的影响

目的:探讨口服大剂量醋酸甲羟孕酮(MPA)对子宫内膜癌间质血管生成的影响。方法:选择经诊断性刮宫证实、未经任何治疗的子宫内膜癌20例,术前口服MPA55mg/d,连续10d。用免疫组化法(LSAB法)以CD34标记血管内皮细胞计数微血管密度(MVD),同时测定用药前后内膜癌标本中PR、VEGF、bFGF的表达,VEGF和bFGF采用计算机图象分析进行半定量分析,比较其用药前后在内膜癌中表达的变化。结果:用药后内膜癌中PR、bFGF的表达及MVD均明显低于用药前,差异有显著性(P<0.05),而用药前后VEGF的表达差异无显著性(P=0.09)。用药前后的内膜癌标本中VEGF和bFGF的表达均与MVD呈正相关(P<0.05)。结论:口服MPA能显著抑制内膜癌间质中的血管生成,其作用可能是通过调节bFGF而不是VEGF的表达实现的。