胎儿体重与宫高腹围孕龄的回归关系

本文根据逐步回归原理,用IBM微型计算机分别探查宫高、腹围、孕周与胎儿体重的关系,求得预测胎儿体重的回归方程:男性体重(克)=71.9×宫高+28.2×腹围+6.2×孕龄天数-3485;女性体重(克)=105.6×宫高+10.2×腹围+1.5×孕龄天数-1678;男女体重合并简化方程=87.5×宫高+20.4×腹围+3.7×孕龄天数-2624。为临床方便,应用后一方程可预测胎儿体重。     

Acute febrile neutrophilic dermatosis (Sweet's syndrome) associated with lymphoma

A case of Sweet's syndrome (acute febrile neutrophilic dermatosis) in a patient in whom lymphoma subsequently developed is presented. The literature concerning Sweet's syndrome in association with malignancy is reviewed. Awareness of this potential association is imperative for the early detection of the neoplasm and for the institution of appropriate follow-up care.     

NF2 gene expression in sporadic meningiomas: Relation to grades or histotypes real time‐PCR study

One of the most common regions involved in the meningiomas tumorigenesis is chromosome 22q where the NF2 gene resides. The deficiency or loss of the NF2 gene product, merlin/schwannomin, plays a role in tumor development and metastatization. Conflicting results have been reported on the prognostic value of merlin in meningiomas. Several studies have indicated NF2 gene inactivation as an early tumorigenic event unrelated to the histological grade or clinical behavior. On the contrary, the NF2 gene alteration rate differs between the different histotypes. A pathogenesis independent from the NF2 gene has been suggested in meningothelial meningiomas. In the present work, we studied the NF2 gene expression through real time‐PCR (RT‐PCR) in 30 meningiomas. The average of the NF2 gene expression of all meningiomas was considered as reference value. The average of expression of WHO grade I and II meningiomas was higher than the average of all meningiomas, whereas that of WHO grade III meningiomas was lower. When we compared the NF2 gene expression in the different meningioma grades we did not note a significant difference (P = 0.698) despite the tendency to decrease from grade I to grade III. The average expression of meningothelial meningiomas was higher than the reference value, and that of non‐meningothelial meningiomas was lower. The difference in NF2 gene expression between meningothelial and non‐meningothelial meningiomas was statistically significant (P = 0.013). Our data supports the finding that alterations in NF2 gene alteration are histotype related but not grade related.     

Development of novel agents for bladder cancer

Traditional treatment options for bladder cancer include transurethral resection and intravesical Bacillus Calmette Guerin for early stage disease and cystectomy or radiation therapy (with or without chemotherapy) for muscle-invasive disease. Platinum-based chemotherapy improves patient outcomes in both the perioperative and metastatic setting. Despite an increase in new therapeutic options over the past decade for many cancer patients, similar advances in bladder cancer are limited. In recent years, an improved understanding of the molecular forces driving bladder cancer development and progression has unfolded. These discoveries create a set of innovative therapeutic opportunities in bladder cancer. This review examines novel anti-cancer agents currently in clinical trials with preclinical rationale to support evaluation in bladder cancer. In addition, strategies to match a patient's tumor to the most appropriate agent are discussed. This may provide a more rational approach to evaluating the role of emerging anti-cancer agents in bladder cancer.     

神经鞘瘤的NF2基因突变分析

目的 分析散发神经鞘瘤发生NF2基因突变及其与临床行为之间的关系。方法 应用PCR－SSCP、DNA测序观察36例神经鞘瘤中发生的NF2基因突变，用Ki-67、PCNA免疫组织化学分析听神经瘤的增殖指数。结果 36例神经鞘瘤中有13个突变，包括缺失、插入所致的移码突变6例，2例无突变，2例反义突变，3例剪接位点改变；其中发生于E2的4例、E4的2例、E6的4例、E13的2例；发生突变的听神经瘤生长指数、增殖指数亦较高。结论 NF2基因突变是神经鞘瘤发生中的常发事件，其与肿瘤的临床行为之间有一定的关系。     

Bone marrow transplantation in accelerated chronic granulocytic leukaemia using dibromomannitol-preconditioning instead of total-body irradiation

In a preliminary study on five patients with accelerated CGL, transplantation of allogeneic matched bone marrow was shown to be feasible without whole-body irradiation. Animal experiments and studies with cells cultured in vitro suggest that the cytocastic drug used to kill leukaemic clones (Myelobromol-Chinoin) does not injure haemopoietic stroma. The administration of this protocol is cheap and easy. Our preconditioning does not, in itself, eradicate the malignant CGL clone immediately: 15-20% of marrow mitoses were Ph1+ one month after transplantation. For this reason, additional cytostatic therapy was given in the course of the 3rd to 6th post-transplant months. No Ph1+ cells were observed from the fourth post-transplant month onwards. Very few severe acute complications were seen and two out of three matched transplanted patients are disease-free 27 + and 13 + months later. On the basis of the developing normal spleen architecture and the changing pattern of circulating NAP score values, particularly the months-long persistence of distinctly low scores, and then the delayed emergence of normal levels, we put forward a hypothesis, emphasizing the role of environmental factors, including the formation of a normal haemopoietic stroma in the successfully transplanted CGL patient.     

抗EB病毒膜抗原单克隆抗体的制备及鉴定

本文将sp_2/o细胞与经脾内注射用0.2mMH2O2激活的B95—8全细胞免疫的BALB/C小鼠脾细胞,用50％PEG4000进行融合,其平均融合率为51.79%,抗体分泌为43.48％。经亚克隆后获得一株能持续分泌抗EB病毒脱抗原的单克隆抗体(以下简称抗EBV—MA单抗)的杂交瘤细胞—B3B7,该细胞的染色体数为111.7±4.76。经ELISA法,间接免疫酶法和间接免疫荧光法证实该单抗不能与EBV—MA(一)的人Rajì细胞发生特异性免疫反应,只能与EBV—MA(+)的人P3HR—1和绒猴的B95—8细胞的反应,而且与H_2O_2激活的B95—8和P_3HX—1细胞的反应强度非常显著地高于与未经H_2O_2激活的B95—8和P_3HR—1细胞的反应强度(P<0.001),同时亦证实特异性荧光主要集中在EBV—MA(+)细胞的细胞膜上,从而表明B_3B_7确实是能分泌抗EBV—MA单抗的杂交瘤细胞。     

腹主动脉—股动脉搭桥术治疗主髂动脉硬化闭塞症

1978年2月～1990年12月间所经治的89例腹主动脉—股动脉搭桥术进行了随访研究。本方介绍了手术方法和治疗结果。经平均3年随访,通畅率为85%,有4例发生股动脉假性动脉瘤。本文提出了移植物阻塞的再手术方法,分析了假性动脉瘤发生的原因。对于主动脉或/和两侧髂动脉阻塞患者,主动脉一两股动脉搭桥术是首选手术方法。