2型糖尿病肾病患者血清肌红蛋白测定的临床意义

目的　探讨 2型糖尿病肾病患者血清肌红蛋白水平。方法　采用放射免疫分析法测定了 32例无糖尿病肾病和 5 2例糖尿病肾病患者血清中肌红蛋白含量 ,并与 35名正常健康人作对照。结果　 2型糖尿病无肾病组和有肾病组血清肌红蛋白水平均高于正常人组 ,尤以糖尿病肾病为甚 (P<0 .0 1) ,糖尿病肾病组与糖尿病无肾病组比较 ,差异亦有显著性 (P<0 .0 1)。结论　 2型糖尿病肾病的发生与发展与肌红蛋白含量密切相关     

Duchenne型肌营养不良症基因携带者检出的血清生化研究

本文测定分析了85名Duchenne型肌营养不良症基因携带者及125名女性正常对照组血清肌酸激酶(CK)、肌酸激酶MB同功酶(CK-MB)、乳酸脱氢酶(LDH)、丙酮酸激酶(PK)、肌红蛋白(Mb),结果表明在携带者检出中,单项指标以Mb或CK为佳,联合应用多项指标提高检出效果,以CK、LDH、Mb的组合最为合理。     

动态观察血清肌红蛋白浓度在推算急性心肌梗塞大小中的价值

本文同时用血清肌红蛋白浓度和肌酸磷酸激酶活性变化对急性心肌梗塞范围进行估测。结果表明,二者高度相关,因发病后肌红蛋白开始升高及达峰值时间均早于肌酸磷酸激酶,故能较其更早地用来估测梗塞面积,指导临床治疗。     

急性心肌梗塞患者血浆内皮素水平变化的意义

动态观察２８例急性心肌梗塞患者血浆内皮素及血清肌红蛋白水平的变化，并同时选择１３例不稳定心绞痛患者和１５例健康人做对对照。结果表明、ＡＭＩ组血中ＥＴ及Ｍｂ水平平均于梗塞２４小时之内达峰值，且随病程延长而逐渐下降，７天后降至正常范围；ＡＭＩ组血浆ＥＴ水平显著高于ＵＡ组及正常组，其中ＵＡ组又明显高于正常组；     

妊高征患者cTnT、Myo测定的临床意义

目的 :探讨监测妊娠高血压综合征患者心肌损伤的实验室量化指标。方法 :连续 5 d采用电化学发光技术检测 63例妊高征患者血清肌钙蛋白 T( c Tn T)及肌红蛋白( Myo)的含量。结果 :重度妊高征患者 c Tn T、Myo异常检出率分别为 66.6%和 44.4% ,明显高于轻、中度妊高征组及正常妊娠组 ,差异有显著性 ( P<0 .0 5 ) ;c Tn T虽上升时间较Myo略晚 ,但上升的幅度更大、持续的时间更长。结论 :重度妊高征患者极易发生心肌细胞受损 ,通过有效的实验室指标可以及早发现、监测病情、积极治疗 ,从而改善母婴预后。     

海洛因所致肾损害与横纹肌溶解症

目的：通过实验研究揭示海洛因成瘾与由之所致肾损害及横纹肌损害之间的关系。方法：应用免疫组织化学及透射电镜扫描的研究方法，对11例海洛因成瘾尸体。肾脏及腓肠肌组织进行透射电镜观察。结果：肾组织免疫组织化学研究。11例中6例检出肌红蛋白成份；对上述尸体肾脏及腓肠肌进行透射电镜扫描观察，不同程度地显示了一定的形态学特征。结论：肾组织中肌红蛋白的检出，揭示了海洛因滥用及其所致肾损害和横纹肌损害之间的关系，指出海洛因所致横纹肌溶解症的存在及其与海洛因成瘾者临床肌痛之间的关系，强调在海洛因所致横纹肌溶解症死者的肾脏组织中，如若采用免疫组织化学的方法可检出肌红蛋白的成份，对海洛因所致其它脏器组织的形态学改变及其成因进行了讨论。     

Myoglobin-facilitated oxygen transport in heterogeneous red muscle tissue

The transport of oxygen in red muscle tissue is influenced by a variety of physical factors: muscle tissue is heterogeneous, oxygen is irreversibly consumed in the muscle cells, and myoglobin is present inside the muscle cells. This is a problem of diffusion with both irreversible and reversible chemical reaction in heterogeneous media. Whereas previously the problem of myoglobin-facilitated oxygen-transport has been considered for homogeneous media, the effect of the tissue heterogeneity on the oxygen transport is investigated here. Simple expressions for the effective oxygen permeability and the effective oxygen consumption for the tissue as a whole are derived as a function of the myoglobin concentration, oxygen consumption inside the cells, the characteristic cell size, cell volume fraction, and physicochemical parameters. The analysis takes into account deviations from chemical equilibrium. Utilizing realistic values for the physicochemical parameters, it is shown that myoglobin-facilitated oxygen transport is significant at low oxygen partial pressures. The oxygen-myoglobin reaction is near but not at equilibrium inside the muscle cell. For large oxygen consumption rates a coupling effect between the reversible and the irreversible reaction takes place which causes an increase in the oxygen transport rate.     

Comparison of the direct electrochemistry of myoglobin and hemoglobin films and their bioelectrocatalytic properties

The electrocatalytic properties of two related proteins, myoglobin (Mb) and hemoglobin (Hb), immobilized in a surfactant film (didodecyldimethylammonium bromide, DDAB) on an electrode were investigated. The direct electrochemistry of the myoglobin/DDAB and hemoglobin/DDAB films was compared and showed one redox couple, two redox couples, and three redox couples, when transferred to strong acidic, weak acidic and basic, and pH 13 aqueous solutions, respectively. The redox couples and their formal potentials are pH dependent. An electrochemical quartz crystal microbalance (EQCM) and cyclic voltammetry were used to study the in situ growth of both DDAB deposition on gold disk electrodes and myoglobin deposition on DDAB film-modified electrodes. The electrocatalytic properties were investigated and showed that the myoglobin/DDAB and hemoglobin/DDAB film modified electrodes are both electrocatalytically active for oxygen reduction, and more H₂O₂ was produced during electrocatalytic reduction using a myoglobin/DDAB film than when using a hemoglobin/DDAB film. The electrocatalysis that was active for l-cystine, N₂O, and 2,2′-dithiosalicylic acid reductions showed an electrocatalytic current developed from the cathodic peak of the redox couple at about ?0.9 V (Fe(II)/Fe(I) redox couple) in neutral and weak basic aqueous solutions. The electrocatalysis that was active for l-cysteine oxidation, showed an electrocatalytic current developed from the cathodic peak of the redox couple at about +0.2 V (Fe(III)/Fe(IV) redox couple). Mb/DDAB and Hb/DDAB film modified electrodes are electrocatalytically active for trichloroacetic acid reduction in weak acidic and basic buffered aqueous solutions through the Fe(II)/Fe(I) redox couple. However, the electrocatalytic current developed from the cathodic peak of the redox couple at a potential of about ?0.1 V (from the Fe(III)/Fe(II) redox couple) in strong acidic aqueous solutions occurs only with higher concentrations of reactant.     

肌酸激酶及其同工酶、肌红蛋白等在电烧伤患者中的应用

目的探讨肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）、α-羟丁酸脱氢酶（HBDH）及心肌肌钙蛋白I（cTnI）、肌红蛋白（Myo）在电烧伤患者中的应用。方法检测电烧伤患者（包括电击伤和电弧伤）的CK、CK．MB、HBDH的含量及cTnI与Myo的阳性率，并与急性冠状动脉综合征（ACS）患者和健康对照组进行比对分析。结果电烧伤患者的CK、CK-MB、HBDH的含量及cTnI与Myo的阳性率均显著高于健康对照组（P〈0．01）；电烧伤患者的CK、CK-MB和Myo的阳性率明显高于ACS组（P〈0．01），HBDH的测定值与ACS组比较差异无统计学意义（P〉0．05），而cTnI的阳性率则低于ACS组。电击伤组Myo的阳性率明显高于电弧伤组（P〈0．01）。讨论CK、CK-MB、HBDH水平及Myo阳性率的增高可能是触电导致肌肉损伤引起的，与心脏损伤不一定有关。cTnI可用于判定电烧伤患者是否出现心脏损害。     

Diagnostic utility of new immunoassays for the cardiac markers cTnI, myoglobin and CK-MB mass

OBJECTIVES: We investigated the diagnostic value of a new system, the Innotrac Aio! immunoassays for troponin, myoglobin and CK-MB, in 270 samples from patients with ACS, after bypass surgery (CABG) or with stable heart failure in comparison to the respective Roche assays. RESULTS: The values of the cardiac markers assessed by the respective assays correlated (cTnT/cTnI Rho = 0.94, myoglobin Rho = 0.87, CK-MB Rho = 0.84). If values were dichotomised, we found a high concordance of test positive and negative classified patients by troponins with the respective assays. CONCLUSION: There is strong evidence that the Innotrac Aio! system for cTnI measurement can be used reliably.