兴义地区97例健康吸烟者的血流变学及甲襞微循环的变化

目的 探讨不同烟龄、吸烟量的吸烟者的血流变学及甲襞微循环的变化及意义。方法 随机选择97例不同烟龄、吸烟量的吸烟者,年龄23－52岁,不吸烟者37例,年龄21－54岁,均为健康体检者。进行全血粘度、血浆比粘度、红细胞压积（HCT）及甲襞微循环的各项积分值（形态、流态、袢周状态、总积分）的统计分析。结果 不同烟龄、吸烟量的吸烟者在全血粘度、血浆比粘度及甲襞微循环的各项积分值与不吸烟者有显著性差异（P＜0．05或P＜0．01）。结论 吸烟者的血流变学及甲襞微循环有显著性改变,尤其是通过甲襞微循环表现得更为突出。     

复方黄芪袋泡饮改善慢性冠脉供血不足的研究

复方黄芪袋泡饮改善慢性冠脉供血不足的研究刘志泉＊陈跃＊李岩飞＊丁煜＊为寻找一种比传统扩血管药物更为有效的中西药复方制剂以改善冠脉血供，降低心绞痛和心肌梗塞的发生率，我们依据中西药理论，结合现代血液流变学和微循环的科研成果，研制了以黄芪、阿司匹林为主要...     

微压传感式毛细血管测压仪

使用自制的微压传感式设备在显微镜下测量甲襞毛细血管压，传感器能精确测出毛细血管血流受阻时的压力，并可自动调零、自动显示和打印。临床使用证明其数据可靠，精确度高且无创，在使用中配以电子半微量天平，保证了仪器的精度、且操作简易，适于临床，优于国外使用的有创性微管插入测压法。     

羟苯磺酸钙对血液流变及血栓形成作用的实验研究

目的研究国产抗糖尿病微血管病变药物羟苯磺酸钙对血小板聚集、血栓形成、血液粘滞度、红细胞脆性以及出血时间等影响 ,为临床应用提供理论依据。方法与结果羟苯磺酸钙0.15、0.21和0.30g/kg,灌胃 ,每日1次 ,连续3d ,可明显抑制ADP和胶原引起的大鼠血小板聚集 ,其抑制率与对照组比较有显著性差异(P<0.01) ;明显降低大鼠血液粘滞度及改善大鼠红细胞中间脆性 ,其作用与对照组比较均有显著性差异(P<0.01)。羟苯磺酸钙0.15、0.30和0.60g/kg灌胃 ,一次给药 ,可明显抑制大鼠颈动静脉旁路血栓形成 ,其作用呈剂量依赖性 ,与对照组比较有显著性差异(P<0.01 ,P<0.05) ;对于小鼠 ,羟苯磺酸钙0.15、0.30和0.60g/kg 灌胃 ,一次给药 ,可明显对抗胶原诱导的血栓形成 ,其降低血栓引起的死亡和偏瘫率呈剂量依赖性 ,与对照组比较有显著性差异(P<0.01 ,P<0.05) ;能明显延长小鼠出血时间 ,与对照组比较有显著性差异(P<0.01)。结论羟苯磺酸钙对于糖尿病微血管病变相关的血液学指标有改善作用。     

金纳多对非协调性异种大鼠肝移植缺血再灌注损伤的防治作用

目的 探讨金纳多对非协调性异种肝移植缺血再灌注损伤的防治作用。方法 对豚鼠和ＳＤ大鼠非协调性异种肝移植进行金纳多（银杏黄酮配糖体）注射液防治的分析,实验分为术前、术后金纳多治疗组和对照组,观察异种肝移植后肝脏和心脏的病理变化及肝脏门脉血流变化。结果 肝移植术后,两组的肝脏和心脏病理变化相似,肝内小血管腔内血小板、中性粒细胞聚集和纤维蛋白沉着造成广泛血栓,引起血管阻塞和组织梗死。心肌细胞破坏,心肌纤     

The biological zero signal in laser doppler fluximetry – origins and practical implications

 This study seeks to identify the origin of the signal, known as biological zero, that is obtained using laser Doppler fluximetry when flow is arrested. It makes specific recommendations on how this signal should be measured and handled when undertaking flow studies. The experiments undertaken using flow models, animal and human tissue, organ preparations and human subjects showed that, although there may be contributions to the no-flow laser Doppler signal from vasomotion, Brownian motion from within the vascular compartment and the effects of cuff compression, the predominant contribution is from Brownian motion arising from the interstitial compartment. The biological zero signal is additive to the flow signal providing conditions within the interstitium remain constant with changes in blood flow. It is thus concluded that the biological zero signal arises from Brownian motion of the macro molecules within the interstitium. This signal should be obtained following 3–5 min of cuff occlusion with inflation applied rapidly with the smallest cuff that is compatible with flow arrest. Biological zero should be measured under each experimental condition and subtracted from the flow signal. Received: 13 August 1998 / Received after revision: 5 November 1998 / Accepted: 9 November 1998     

开音片对小鼠微循环影响的实验研究

目的：通过观察小鼠耳廓微循环的变化,探讨开音片活血化瘀的作用。方法：采用ＸＹ－３型微循环显微镜来观察小鼠耳廓微循环的变化。结果：小鼠耳廓微循环红细胞解聚明显,血流速度加快,微静脉口径增大,线流比例增加。结论：开音片能明显改善小鼠耳廓微循环状况。     

Major organ function under mechanical support: comparative studies of pulsatile and nonpulsatile circulation

We examined a major organ function during 3 h biventricular assisted circulation after acute myocardial infarction model in the pig. In left ventricular circulation, the outflow cannula was placed in the ascending aorta and an inflow cannula through the mitral valve in the left ventricle. A pump (pulsatile group, Zeon Medical, Inc., Tokyo, Japan and nonpulsatile group, Nikkiso HPM-15, Nikkiso, Inc., Tokyo, Japan) was connected to each cannula. In right ventricular circulation, the outflow cannula was placed in the pulmonary artery and an inflow cannula in the right ventricle. The right ventricular circulation was supported by a nonpulsatile pump (Nikkiso HPM-15). The items measured were the regional blood flows of the cortex and medulla in the kidney, white matter and gray mater in brain, and liver; renal arterial flow; carotid arterial flow; portal vein flow; common hepatic arterial flow; arterial ketone body ratio (AKBR); and lactate/pyrubic acid (L/P). In the pulsatile group, the renal cortical blood flow increased, and the medulla blood flow decreased. On the other hand, in the nonpulsatile group, both regional blood flows decreased. That means that in the pulsatile assisted group intrarenal redistribution improved rather than in the nonpulsatile assisted group. In addition the liver regional blood flow, AKBR, and L/P showed significant differences between the pulsatile and nonpulsatile groups. On the other hand, the white matter and gray matter regional blood flows and carotid arterial flow did not show significant differences between the groups. The results of our study indicated that pulsatile circulation produced superior circulation in the kidney and liver, and microcirculation on the cell level was superior as well in early treatment of acute heart failure.     

Assessment of efficacy of bothropic antivenom therapy on microcirculatory effects induced by Bothrops jararaca snake venom.

Intravenous administration of antibothropic antivenom (BAv) neutralises the systemic effects, but does not efficiently reverse the local symptoms elicited by the Bothrops jararaca venom (BjV). The mechanisms involved in this poor protection have not been clarified. In this work, intravital microscopy studies were carried out to determine the efficacy of different schedules of BAv treatment on local effects evoked by topical application of BjV in the microcirculatory network of the internal spermatic fascia of Wistar rats. Results demonstrated that BAv administration 15 min before, simultaneously with, or 15 min after BjV application did not totally reverse the local symptoms, represented by disturbances of coagulation, development of haemorrhage lesions, vascular permeability increase and increment on leukocyte-endothelium interactions. This lack of effectiveness neither reflects an inadequate amount of specific antibodies in the antivenom against toxins responsible for local effects nor an insufficient dose of circulating BAv during the assays. Administration of fluorescein isothiocyanate (FITC) labelled-BAv showed the dynamics of distribution of the antivenom in the microcirculatory network. Images obtained from prior and simultaneously treated animals showed that the antivenom remains at luminal side of vessels before venom application, and the latency time to antivenom leakage is coincidental to that for local effects evoked by the venom. In addition, images from posterior treatment demonstrated that the intense alterations in the microcirculatory network impair antivenom distribution at the site of injection. Together, our data show that the lack of effectiveness of antivenom therapy is due to impaired and delayed venom and antivenom interaction at the site of injury.     

胸肩峰皮瓣微循环监测及临床应用

目的　监测胸肩峰皮瓣微循环血流动态变化及其规律 ,为选择最佳断蒂时间提供可靠客观量化指标。　方法　应用激光多普勒血流计 (LDF)对 12例胸肩峰皮瓣转移面中点于术后 2～ 7天逐日进行微循环监测 ,皮瓣移植后第 4天起记录皮瓣蒂部阻断前、后LDF的PU(灌注单位 )值变化情况。　结果　胸肩峰皮瓣微循环血流变化可分为 2期 ,即术后前 4天的骤升期PU值分别为 3 5±1 3、4 5± 1 7、6 7± 2 9;术后 4～ 7天的平台期PU值分别为 7 4± 2 0、7 6± 2 3、7 3± 1 6。本组断蒂时间为术后 6～ 8天。　结论　胸肩峰皮瓣在转移修复面部缺损时 ,其微循环血流变化在平台期后期时 ,当阻断皮瓣蒂部后其PU值恢复到或超过阻断前水平的 5 0 %时进行断蒂 ,皮瓣全部成活。