Comparing the ESA HPLC total homocysteine assay with electrochemical detection to the CDC in-house HPLC assay with fluorescence detection

Background: Environmental science has developed a simple high performance liquid chromatography (HPLC) assay with electrochemical detection for total homocysteine (tHcy) measurement that does not require derivatization of free thiols. We evaluated this method and compared it with the CDC HPLC assay with fluorescence detection (FD). Methods: tHcy is measured after reduction of disulfides/protein-bound thiols and protein precipitation using four channels of an ESA CoulArray detector. -homocystine is used as calibrator, penicillamine as internal standard. Results: Aqueous calibration of the ESA assay resulted in overestimation of tHcy by 30% compared to the HPLC-FD method. Calibration in plasma alleviated the matrix effect. The within- (n=3) and between-run (n=20) imprecision was <6%, the linearity up to 100 μmol/l was excellent, and the recovery of tHcy added to plasma was nearly complete (98.7%±2.3%). Good correlation was observed between both methods for 266 plasma samples. The ESA assay showed a minimal negative bias of 0.28 μmol/l (3.3%). Conclusion: The ESA tHcy assay performed well in terms of accuracy and precision, and showed good agreement with the CDC HPLC-FD assay when calibrated in plasma. The major advantage of this assay is that it does not require sample derivatization. Disadvantages include instability of the prepared samples for prolonged storage and matrix effects.     

矿泉浴治疗慢性下腰痛的疗效评价

目的　评价兴城矿泉浴治疗慢性下腰痛 (CLBP)的疗效。方法　 78例CLBP患者随机分为矿泉浴组和淡水浴组 (各 3 9例 ) ,两组均保持入组前所用的其余保守治疗方法不变。矿泉为兴城矿泉 ,淡水为兴城饮用自来水 ,均采用全身浸浴法 ,水温 3 8～ 40℃ ,每日 1次 ,每次 2 5～ 3 0分钟 ,2 0次 1个疗程 ,在疗程开始前和疗程结束后各评定腰腿痛积分 1次。结果　矿泉浴组腰腿痛总积分治疗前 3 0 .2 4± 4.87,治疗后 9.64± 1 .0 5 ,差异显著 (P <0 .0 1 ) ;淡水浴组腰腿痛总积分治疗前 2 9.75± 4.47,治疗后 1 9.98± 3 .5 6,差异显著 (P <0 .0 5 )。两组积分改善值除椎管内积分外 ,矿泉浴组均明显高于淡水浴组 (P <0 .0 1 )。结论　兴城矿泉浴能明显缓解慢性下腰痛患者的症状和体征。     

Questionnaire-based analysis of mefloquine chemoprophylaxis for malaria in a Japanese population

Although mefloquine is the only drug licensed for malaria chemoprophylaxis in Japan, there have been few reports describing the effects of and adverse events in the prophylactic usage of mefloquine in a Japanese population. We therefore performed a questionnaire-based study in 21 travelers who were prescribed mefloquine for malaria chemoprophylaxis between October 2001 and December 2003. The study revealed that only 8 out of 21 (38.1%) of the travelers could complete the prophylaxis schedules. Another 8 travelers (38.1%) with incomplete adherence stated that they did not take mefloquine because of either actually experienced or anticipated adverse events. Twelve of the 16 travelers (75.0%) who took mefloquine complained of at least one adverse event probably related to mefloquine. As an overall impression about mefloquine chemoprophylaxis, 14 of the 21 travelers stated that they would take mefloquine again for the next travel to malaria-endemic areas, although 5 of them were concerned about adverse events. These results suggest that, although mefloquine is an indispensable drug for malaria prevention, other effective and well-tolerated chemoprophylactic antimalarials should be available for Japanese travelers who do not tolerate mefloquine.     

漂浮疗法对大学生免疫功能的影响

目的:采用淋巴细胞转化率为指标研究漂浮疗法对个体免疫功能的影响。方法:以淋巴细胞转化率作为免疫功能变化指标。被试为43名大学生(男22名,女21名),年龄18～24岁。被试随机分为4组,其中前3组均采用漂浮疗法,但具体过程略有不同。第1组:漂浮,播放漂浮治疗专用音乐;第2组:漂浮,通过内部通讯系统进行放松指导;第3组:漂浮,无任何听觉刺激;第4组为对照组。每人完成4次治疗。结果:各组被试治疗前的淋巴细胞转化率没有显著性差异(F=0.733P=0.539)。采用漂浮治疗的各组中,只有第3组淋巴细胞转化率在治疗前后有显著性差异犤(91.7±4.2)%vs(84.8±7.2)%犦,而对照组治疗前后该项检查的结果也有显著性差异犤(93.0±2.8)%vs(89.3±4.2)%犦。第3组被试治疗前后淋巴细胞转化率变化显著大于第2组被试(t=2.168,P=0.043)。男女被试的淋巴细胞转化率在治疗前分别为87.68%和86.95%,两者没有显著性差异(t=0.314,P=0.755)。男女被试的淋巴细胞转化率在治疗前后的变化没有显著性差异(t=0.503P=0.620)。结论:漂浮疗法的确可以提高被试的细胞免疫功能,但是漂浮治疗中使用额外的听觉刺激可能会影响到改善免疫力的作用。性别对于淋巴细胞转化率变化没有显著影响。该研究的结果在应用于临床前,尚需要进一步研究。     

小柴胡汤加味联合经鼻肠梗阻导管对粘连性肠梗阻治疗效果

目的:对小柴胡汤加味联合经鼻肠梗阻导管治疗粘连性肠梗阻的效果行回顾性分析。方法:按纳入标准收集湖南中医药大学第一附属医院普外微创科于2018年4月至2019年4月期间符合粘连性肠梗阻诊断的患者50例。随机分为2组,对照组(25例)和实验组(25例)。对照组行经鼻肠梗阻导管置入术治疗,术后予以常规治疗,实验组在常规治疗的基础上给予了小柴胡汤加味方治疗。2组均3天复查一次相关实验室指标。比较两组排气排便恢复时间、开始进食时间、导管的拔除时间、住院时间等指标,并比较两组患者炎性指标变化以及治疗结果。结果:对照组患者平均的排气排便恢复时间、腹痛腹胀消失时间、进食时间、气液平消失时间、导管拔除时间、平均住院时间普遍长于实验组;实验组患者治疗后白细胞(WBC)计数、C反应蛋白(CRP)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)等炎性指标的水平较对照组明显降低;实验组的总有效率高于对照组。结论:小柴胡汤加味联合经鼻肠梗阻导管对治疗粘连性肠梗阻比单纯使用经鼻肠梗阻导管治疗效果好。     

Skeletal muscle BOLD MRI: from underlying physiological concepts to its usefulness in clinical conditions

Blood oxygenation-level dependent (BOLD) MRI has gained particular attention in functional brain imaging studies, where it can be used to localize areas of brain activation with high temporal resolution. To a higher degree than in the brain, skeletal muscles show extensive but transient alterations of blood flow between resting and activation state. Thus, there has been interest in the application of the BOLD effect in studying the physiology of skeletal muscles (healthy and diseased) and its possible application to clinical practice. This review outlines the potential of skeletal muscle BOLD MRI as a diagnostic tool for the evaluation of physiological and pathological alterations in the peripheral limb perfusion, such as in peripheral arterial occlusive disease. Moreover, current knowledge is summarized regarding the complex mechanisms eliciting BOLD effect in skeletal muscle. We describe technical fundaments of the procedure that should be taken into account when performing skeletal muscle BOLD MRI, including the most often applied paradigms to provoke BOLD signal changes and key parameters of the resulting time courses. Possible confounding effects in muscle BOLD imaging studies, like age, muscle fiber type, training state, and drug effects are also reviewed in detail.     

开腹手术下行胃穿孔修补术治疗胃穿孔的临床效果

目的：研究并分析开腹手术下行胃穿孔修补术的临床效果。方法依据纳入、排除标准,选取本院2013年1月~2014年9月收治的90例胃穿孔患者为研究对象,按照随机数字表法将其分为研究组和对照组各45例。对照组给予内科常规治疗,研究组在内科常规治疗的基础上采取开腹手术下行胃穿孔修补术治疗。比较两组的临床治疗效果。结果研究组患者的住院时间、起床时间、下床活动时间均短于对照组,差异有统计学意义（P﹤0.05）。研究组治疗总有效率为97.8%,高于对照组的64.4%,差异有统计学意义（P﹤0.05）。结论开腹手术下行胃穿孔修补术的临床效果优于内科常规治疗,住院时间、起床时间、下床活动时间均明显缩短,术后并发症少,患者愿意接受,值得临床推广应用。     

连花清瘟颗粒联合利巴韦林和康复新液治疗疱疹性口腔炎的疗效观察

目的：观察连花清瘟颗粒联合利巴韦林和康复新液治疗疱疹性口腔炎的临床疗效。方法选择2012年1~10月份在医院儿科门诊进行诊治的疱疹性口腔炎的患儿80例,完全随机平分为治疗组和对照组各40例,2组除给予维生素 C 和维生素 B2等支持、对症治疗以外,均给予利巴韦林注射液静脉滴注10~15mg·kg _1·d _1,分2次给药和口服康复新液,＜3岁患儿每次5ml,＞3岁患儿每次10ml,均3次∕ d,治疗组患儿在此治疗基础上加用连花清瘟颗粒口服（石家庄以岭药业股份有限公司,6g∕袋）,≤3岁3g∕次,3次∕ d;＞3岁 6g∕次,3次∕ d,均治疗5d。结果治疗组总有效率为97,5％明显高于对照组的87,5％（p ＜0,01）,治疗组热退时间,口腔炎疱疹溃疡愈合时间为（1,4±0,7）d、（2,9±0,6）d 比较对照组的（3,2±1,2）d、（3,7 ± 0,9）d 明显缩短,差异有统计学意义（p ＜0,01）。结论连花清瘟颗粒联合利巴韦林和康复新液治疗疱疹性口腔炎,能迅速缓解临床症状,疗效确切,值得临床推广和应用。