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121.
目的 研究抗人卵巢癌 (ovariancarcinoma ,oc)×抗人CD3×抗CD2 8VH 单链三特异抗体(singlechaintrispecificantibody,scTsAb)在大肠杆菌中的可溶表达与纯化及纯化后产物的活性测定 ,从而为其应用于卵巢癌治疗的临床研究打下基础。方法 将已构建的scTsAb表达载体转化大肠杆菌BL2 1(DE3)Star菌株 ,采用低温 (30℃ )、低剂量IPTG(0 .2mmol L)诱导 ,进行胞内可溶表达。根据抗卵巢癌三特异抗体 (ocTsAb)等电点较高 (pI9.0 ) ,而菌体蛋白大多为酸性蛋白的特点 ,利用DEAE弱阴离子交换层析(pH8.0 )进行一步纯化 ,并利用ELISA及FACS的方法检测纯化后抗卵巢癌三特异抗体的活性。结果 (1)SDS PAGE鉴定低温诱导时可溶比例达到 5 6 %。 (2 )绝大多数菌体蛋白被DEAE层析柱吸附 ,而抗卵巢癌三特异抗体在穿透液中流出 ,SDS PAGE检测纯度达到 90 %。 (3)ELISA结果显示纯化后的抗卵巢癌三特异抗体与重组CD2 8纯抗原 ,Jurkat(CD3 )细胞膜提取抗原 ,SKOV3细胞膜提取抗原均有特异性结合。 (4 )FACS结果证明纯化后的抗卵巢癌三特异抗体与Jurkat(CD3 )活细胞、SKOV3活细胞有特异性结合。结论 低温诱导胞内可溶表达的抗人卵巢癌×抗人CD3×抗CD2 8VH 单链三特异抗体经弱阴离子交换层析一步纯化后仍保持原有免疫学活性 ,这 相似文献
122.
One hundred and sixty-three healthy Chinese subjects of both sexes were studied for serum paraoxonase (PON) polymorphism, and levels of lipids and apolipoproteins in order to examine effects of PON alleles on these parameters. The level of serum triglyceride was significantly higher in high activity allele (PON*B) compared with that in low activity allele (PON*A) in both sexes (P less than 0.01). The subjects with PON A had significantly higher LDL cholesterol (P less than 0.05) and lower Apo A-II and ApoB levels. The influence of serum paraoxonase on serum lipids was estimated further by Spearman's rank correlation. In the males, there was a significant negative correlation of serum paraoxonase activity with total (P less than 0.05) and LDL (P less than 0.01) cholesterol levels, and positive correlation with HDL cholesterol and Apo A-II levels (P less than 0.05). Serum paraoxonase activity had a high positive correlation with serum triglyceride levels in both sexes (P less than 0.001). Serum ApoB level had a positive correlation with the enzyme activity only in females (P less than 0.01). The allelic effect of PON on these parameters was studied by multiple regression analysis. The high activity allele (PON*B) was associated with higher serum triglyceride level (P less than 0.001) and ApoB (P less than 0.001), while it had lowering influence on total cholesterol (P less than 0.05) and LDL cholesterol (P less than 0.005) in men. The average allelic effect of PON was found to be about 22% for serum triglycerides, 11% for LDL cholesterol, 14% for Apo A-II and 19% for Apo B in the present study. This study suggests a possible significant role of serum paraoxonase alleles in the metabolism of serum lipids and apolipoproteins. 相似文献
123.
T. N. Ivanova E. D. Polyakova A. M. Olfer'ev N. V. Perova 《Bulletin of experimental biology and medicine》1998,125(5):505-508
The rate of cholesterol synthesis in peripheral blood lymphocytes and plasma lipid and lipoprotein spectra are studied in
patients with isolated hypercholesterolemia and combined hyperlipidemias (IIa and IIb hyperlipidemias according to Frederickson
classification).14C-acetate incorporation into cholesterol in peripheral blood lymphocytes is considerably higher in patients with type IIb
hyperlipidemia. Lipanthyl-200M reduces the rate of cholesterol synthesis in lymphocytes of both groups. A direct correlation
is established between serum triglyceride level and the rate of cholesterol synthesis.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 125, No. 5, pp. 569–573, May, 1998 相似文献
124.
慢性肾功能衰竭红细胞变形能力与红细胞膜的研究 总被引:1,自引:0,他引:1
本文报道了慢性肾功能衰竭患者红细胞的变形能力、红细胞膜胆固醇与磷脂比值及膜微粘度。结果表明,红细胞可变形能力明显降低,膜胆固醇与磷脂比值及膜微粘度明显增加,提示红细胞变形能力可能与红细胞膜的流动性有关。 相似文献
125.
探讨低分子肝素对缺血再灌注大鼠肾组织核因子-κB(NF-κB)表达的影响。建立大鼠IRI模型,健康WistaI大鼠80只随机分为正常对照组、假手术组、模型未治疗组、LMWH治疗组,后两组又分别分为术后1、3、6、24h组。检测各组血清肌酐(Scr)水平及中性粒细胞(PMNs)细胞间黏附分子-1(ICAM-1)表达;通过光镜和免疫组织化学方法观察各组大鼠肾组织形态学及趋化因子NF-κB表达变化。结果表明:(1)肾缺血再灌注未治疗组造模后1h,Scr水平虽然没有明显变化,但ICAM-1、NF-κB表达增多,肾小管坏死积分值亦较假手术组明显增加(P〈0.01);(2)缺血再灌注6h以后,两组Scr浓度明显增高(P〈0.01),但LMWH治疗组SCr、ICAM-1、NF-κB表达水平及肾小管坏死积分值均明显低于模型未治疗组(P〈0.05);(3)肾组织中NF-κB表达与肾小管损伤积分值呈现良好的相关性(r=0.71,P〈0、01);而NF-κB与ICAM-1间则呈现显著正相关(r=0.62,P〈0.05)。由此说明:(1)ICAM-1、NF-κB在肾缺血再灌注早期的瞬时表达,可能参与了炎症早期的白细胞迁移与浸润,与肾损伤的发生密切相关;(2)LMWH可通过减少ICAM-1及NF-κB的表达,阻抑炎症反应过程,减轻肾组织损伤。 相似文献
126.
类固醇激素合成急性调节蛋白(StAR)在胆固醇的代谢中发挥重要的作用。近期研究表明StAR同样表达于肝脏组织中,通过调节胆汁酸的合成,增加胆固醇的排泄。这为脂肪肝等脂类代谢异常类疾病的防治提供了一条新的途径。 相似文献
127.
Nobuhiko Okamoto Mashiro Nakayama Chie Narahara Han-suk Kim Masashi Fujioka Isao Imada Tatsuya Arai Soichiro Toda 《Journal of human genetics》1997,42(3):441-444
Summary Mevalonic acidemia is a rare metabolic disorder due to mevalonate kinase deficiency which affects the biosynthesis of cholesterol
and nonsterol isoprenes. We report the first case of Japan. The clinical course is characterized by intrauterine growth retardation,
postnatal growth failure, intractable diarrhea, liver dysfunctions and death at three months of age. Dysmorphic features including
triangular face, protrusion of forehead, hypertelorism, low set ears and micrognathism were noted. High mevalonic acid level
was found by GC/MS. 相似文献
128.
Mahmoud S. El-Sayed Angelheart J.M. Rattu 《European journal of applied physiology》1996,73(1-2):88-92
This study examined the effect of prolonged submaximal exercise followed by a self-paced maximal performance test on cholesterol (T-Chol), triglycerides (TG), and high-density lipoprotein cholesterol (HDLC). Nine trained male athletes cycled at 70% of maximal oxygen consumption for 60 min, followed by a selfpaced maximal ride for 10 min. Venous blood samples were obtained at rest, at 30 and 60 min during submaximal exercise, and immediately after the performance test. Lactic acid, haematocrit (Hct), haemoglobin (Hb), T-Chol and TG were measured in the blood, while plasma was assayed for HDL-C. Plasma volume changes in response to exercise were calculated from Hct and Hb values and all lipid measurements were corrected accordingly. In order to ascertain the repeatability of lipid responses to exercise, all subjects were re-tested under identical testing conditions and experimental protocols. When data obtained during the two exercise trials were analysed by two-way ANOVA no significant differences (P > 0.05) between tests were observed. Consequently the data obtained during the two testing trials were pooled and analysed by one-way ANOVA. Blood lactic acid increased non-significantly (P > 0.05) during the prolonged submaximal test, but rose markedly (P < 0.05) following the performance ride. Lipid variables ascertained at rest were within the normal range for healthy subjects. ANOVA showed that blood T-Chol and TG were unchanged (P > 0.05), whereas HDL-C rose significantly (P < 0.05) in response to exercise. Post hoc analyses indicated that the latter change was due to a significant rise in HDL-C after the performance ride. It is concluded that apparent favourable changes in lipid profile variables occur in response to prolonged submaximal exercise followed by maximal effort, and these changes showed a good level of agreement over the two testing occasions. 相似文献
129.
Patterson Thomas L.; Sallis James F.; Nader Philip R.; Kaplan Robert M.; Rupp Joan W.; Atkins Catherine J.; Senn Karen L. 《Journal of pediatric psychology》1989,14(2):277-292
A number of studies have demonstrated that physiological andbehavioral cardiovascular disease (CVD) risk factors aggregatewithin families. This fact, and the potential mediating rolethat the family plays in behavior change, have led to the developmentof family-based CVD risk reduction programs, including the SanDiego Family Health Project. The aggregation of behavioral,physiological, and cognitive changes within families was assessedduring a 1-year intervention. We found evidence of modest butsignificant aggregation of change. There was more aggregationof change in behavioral variables than in physiological or cognitivevariables. More significant correlations were found among 3-dayfood record measures than among 24-hour recall dietary measures,suggesting an influence of assessment method. Aggregation ofchange within families was stronger within generations thanacross generations. These data point to the importance of involvingall age groups in health promotion programs. 相似文献
130.
M. Flesch A. Sachinidis Y. D. Ko K. Kraft H. Vetter 《Journal of molecular medicine (Berlin, Germany)》1994,72(12):944-950
In recent years there have been many studies demonstrating a correlation between increased arterial blood pressure and altered lipid profiles, and there has been an especially positive correlation between high cholesterol levels and blood pressure. There are differences between the various reports that are important. In our study the lipid distribution in 105 hypertensive patients with mild or moderate arterial hypertension according to WHO criteria without clinically or ultrasonographically apparent atherosclerosis was compared to the lipid distribution in 65 age-matched healthy persons. On the epidemiological level a significant, positive association was found between LDL serum levels (P 0.001), Apo B serum levels (P 0.001), serum triglyceride levels (P 0.05) and VLDL serum levels (P 0.01) and arterial hypertension. However, in contrast to recent reports, no significant difference was found between total serum cholesterol levels in normotensives and hypertensives, and there was no difference in HDL serum levels. No evidence could be found for a significant increase in lipoprotein (a) serum levels in hypertensives.Abbreviations LDL
low density lipoprotein
- VLDL
very low density lipoprotein
- HDL
high density lipoprotein
- Apo B 100
apolipoprotein B 100
- Apo A I
apolipoprotein A I
Correspondence to: H. Vetter 相似文献