利用RIBA试验确定HCV抗体检测的cut- off值及比较分析

目的 利用重组蛋白免疫印迹分析(RIBA)实验,制定本实验室所用的日本希森美康公司的HISCL-5000型化学发光免疫分析仪及配套的相关试剂检测丙型肝炎病毒抗体(抗-HCV)的cut-off值。方法 在2015年10月至2016年9月所检测的19 341例患者标本中收集抗-HCV阳性标本80例,进行RIBA确认实验后进行分析,再利用ROC曲线计算出cut-off值。结果 在本室HCV抗体阳性率为0.41%的数据中,RIBA结果可分为3种,其中25例(31.3%)为阴性,18例(22.5%)为不确定,37例(46.2%)为阳性。当截断值为3.95 COI时,灵敏度为81.1%,特异性为95.1%。另外当特异性为100%时,截断值为5.25 COI,灵敏度为73%。结论 希森美康HISCL-5000型化学发光免疫分析仪及配套的相关试剂检测丙型肝炎病毒抗体(抗-HCV)与RIBA结果间呈正相关;当cut-off值为3.95 COI时,是一个适合本实验室的cut-off值。     

庚型肝炎——庚型肝炎病毒感染对慢性肝炎发生发展的影响

目的：探讨庚型肝炎病毒（GBvirusC）感染对慢性肝炎发生发展的影响及临床意义。方法：采用免疫组化方法对143例慢性肝炎患者肝组织标本进行GBvirusC非结构蛋白5（NSS）抗原的检测，结合组织学改变进行分析。结果：慢性肝炎肝组织中GBvirusC总检出率为24．5％，其中单纯GbvirusC感染占11．9％；单纯GBvirusC感染和病原不明组炎症活动度程度较轻，只有8．9％达到3—4级，显著低于HBV／HCV感染病例和GBviru SC＆HBV／HCV混合感染组（26．5％）。结论：GBvirusC的致病性值得怀疑或者仅具有较弱的致病性，GBvirusC可能不是慢性肝炎的主要致病因子，它和HBV／HCV混合感染并不加重肝损害程度，不影响慢性肝炎的发生发展。     

358例下颌阻生智齿拔除方法对比

阻生智齿是人类长期进化过程中,由于食物的精制致使咀嚼功能降低,颌骨的发育逐渐退缩,使第3磨牙（智齿）失去萌出的正常位置所产生的。所以,智齿以各种各样的形态出现在颌骨内：垂直低位、水平位、颊舌向生长……。     

云南省3株柯萨奇病毒A组10型分离株全基因组序列分析

目的 分析手足口病相关的柯萨奇病毒A组10型(coxsackievirus A10,CV-A10)云南省分离株的基因组特征.方法 对云南省2009年和2015年3例手足口病患儿临床样本中分离到的3株CV-A10分离株A103/YN/CHN/2015、A72/YN/CHN/2015和R09191/YN/CH N/2009...     

211例原发性肝癌的临床特点与预后的关系

原发性肝癌(PHC)是我国恶性肿瘤中常见的种类之一,目前在恶性肿瘤发病中占第三位。已证实HBV感染是导致PHC的主要原因,另有丙肝病毒HCV感染等多种病因,PHC的临床特点和预后也出现多种变化。本文对211例原发性肝癌的病因、肝肾生化检查指标、门脉高压等并发症及对患者预后影响进行探讨。     

Impact of cigarette smoking on response to interferon therapy in chronic hepatitis C Egyptian patients

AIM: Smoking may affect adversely the response rate to interferon-α. Our objective was to verify this issue among chronic hepatitis C patients. METHODS: Over the year 1998, 138 chronic hepatitis C male Egyptian patients presenting to Cairo Liver Center, were divided on the basis of smoking habit into: group I which comprised 38 smoker patients (>30 cigarettes/d) and group II which included 84 non-smoker patients. Irregular and mild smokers (16 patients) were excluded. Non eligible patients for interferon-~ therapy were excluded from the study and comprised 3/38 (normal ALT) in group I and 22/84 in group II (normal ALT, advanced cirrhosis and thrombocytopenia). Group I was randomly allocated into 2 sub-groups: group Ia comprised 18 patients who were subjected to therapeutic phlebotomy while sub-group Ib consisted of 17 patients who had no phlebotomy. In sub-group Ia, 3 patients with normal ALT after repeated phlebotomies were excluded from the study. Interferon-α2b 3 MU/TIW was given for 6 mo to 15 patients in group Ia, 17 patients in group Ib and 62 patients in group II. Biochemical, virological end-of- treatment and sustained responses were evaluated.RESULTS: At the end of interferon-α treatment, ALT was normalized in 3/15 patients (20%) in group Ia and 2/17 patients (11.8%) in group Ib compared to17/62 patients (27.4%) in group II (P=0.1). Whereas 2/15 patients (13.3%) in group Ia. and 2/17 patients (11.8%) in group Ib lost viraemia compared to 13/62 patients (26%) in group II(P=0.3). Six months later, ALT was persistently normal in 2/15 patients (13.3%) in group la and 1/17 patients (5.9%) in group Ib compared to 9/62 patients (14.5%) in group Ⅱ (P= 0.47). Viraemia was eliminated in 1/15 patients (6.7%) in group Ia and 1/17 patients (5.9%) in group Ib compared to 7/62 patients (11.3%) in group Ⅱ, but the results did not mount to statistical significance (P = 0.4). CONCLUSION: Smokers suffering from chronic hepatitis C tend to have a lower response rate to interferon-α compared to non-smokers. Therapeutic phlebotomy improves the response rate to interferon-α therapy among this group.     

沧州地区合格献血者HBV、HCV和HIV核酸检测结果

输血后病毒感染的主要原因有：病毒感染者＂窗口期＂献血;病毒变异;免疫静默感染;以及人工操作错误,其中病毒感染者＂窗口期＂献血是主要原因。目前我国大部分血站采用2个不同厂家ELISA试剂筛查献血者传染病指标。由于该检测方法的局限性,使乙肝、丙肝、艾滋＂窗口期＂造成临床输血传播疾病风险依旧存在。核酸检测（NAT）是对病原体核酸直接检测,病毒感染数天即能检出极微量的病毒核酸,不仅能大大缩短＂窗口期＂且能避免免疫静默感染发生。     

丙型肝炎

载脂蛋白B基因多态性及血脂水平与慢性丙型肝炎的相关性；HCV核心蛋白在酵母双杂交系统中的表达及自激活作用检测；HCVRNA在外周血单个核细胞中的复制与丙肝慢性化及复发的关系；丙型肝炎病毒持续感染对体外培养人胎盘合体滋养层细胞生物学性状的影响     

加亚嘉西科逆转录病毒研究进展

从加亚嘉西科逆转录病毒(JSRV)的基因组、JSRV的致瘤信号传导途径、JSRV与宿主共进化方面综述了JSRV的研究进展.JSRV引起的羊肺腺瘤(SPA)与人的细支气管肺泡癌(BAC)极相似,通过对SPA的研究,不仅为BAC早期诊断及预防该病提出新方法,而且为揭示BAC的分子发病机制及探索其基因治疗提供新思路.