全文获取类型
收费全文 | 13711篇 |
免费 | 872篇 |
国内免费 | 346篇 |
专业分类
耳鼻咽喉 | 155篇 |
儿科学 | 981篇 |
妇产科学 | 298篇 |
基础医学 | 1794篇 |
口腔科学 | 448篇 |
临床医学 | 881篇 |
内科学 | 2060篇 |
皮肤病学 | 193篇 |
神经病学 | 765篇 |
特种医学 | 204篇 |
外国民族医学 | 1篇 |
外科学 | 2345篇 |
综合类 | 1249篇 |
预防医学 | 1511篇 |
眼科学 | 90篇 |
药学 | 1115篇 |
1篇 | |
中国医学 | 202篇 |
肿瘤学 | 636篇 |
出版年
2024年 | 21篇 |
2023年 | 196篇 |
2022年 | 332篇 |
2021年 | 389篇 |
2020年 | 362篇 |
2019年 | 389篇 |
2018年 | 369篇 |
2017年 | 399篇 |
2016年 | 415篇 |
2015年 | 494篇 |
2014年 | 792篇 |
2013年 | 975篇 |
2012年 | 697篇 |
2011年 | 809篇 |
2010年 | 619篇 |
2009年 | 634篇 |
2008年 | 654篇 |
2007年 | 680篇 |
2006年 | 605篇 |
2005年 | 525篇 |
2004年 | 453篇 |
2003年 | 388篇 |
2002年 | 346篇 |
2001年 | 294篇 |
2000年 | 303篇 |
1999年 | 236篇 |
1998年 | 250篇 |
1997年 | 225篇 |
1996年 | 182篇 |
1995年 | 200篇 |
1994年 | 177篇 |
1993年 | 160篇 |
1992年 | 118篇 |
1991年 | 153篇 |
1990年 | 123篇 |
1989年 | 107篇 |
1988年 | 105篇 |
1987年 | 97篇 |
1986年 | 105篇 |
1985年 | 97篇 |
1984年 | 83篇 |
1983年 | 37篇 |
1982年 | 48篇 |
1981年 | 52篇 |
1980年 | 39篇 |
1979年 | 36篇 |
1978年 | 27篇 |
1977年 | 26篇 |
1976年 | 19篇 |
1972年 | 19篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
101.
Classic Ehlers-Danlos syndrome (EDS) is characterized by fragile and hyperextensible skin, atrophic scarring, and joint hypermobility. Mutations in the COL5A1 and the COL5A2 gene encoding the alpha1(V) and the alpha2(V) chains, respectively, of type V collagen have been shown to cause the disorder, but it is unknown what proportion of classic EDS patients carries a mutation in these genes. We studied fibroblast cultures from 48 patients with classic EDS by SDS-PAGE for the presence of type V collagen defects. An abnormal collagen pattern was detected in only 2 out of 48 cell lines, making this a poor method for routine diagnostic evaluation. A total of 42 out of 48 (88%) patients were heterozygous for an expressed polymorphic variant in COL5A1. cDNA from 18 (43%) of them expressed only one COL5A1 allele. In 37 patients, the COL5A1/A2 genes were then analyzed by SSCP and conformation sensitive gel electrophoresis (CSGE). A total of 26 patients that were mutation-negative after SSCP/CSGE screening were reanalyzed by dHPLC. In addition, 11 other patients were analyzed by dHPLC only. In total, 17 mutations leading to a premature stop codon and five structural mutations were identified in the COL5A1 and the COL5A2 genes. In three patients with a positive COL5A1 null-allele test, no causal mutation was found. Overall, in 25 out of 48 patients (52%) with classic EDS, an abnormality in type V collagen was confirmed. Variability in severity of the phenotype was observed, but no significant genotype-phenotype correlations emerged. The relatively low mutation detection rate suggests that other genes are involved in classic EDS. We excluded the COL1A1, COL1A2, and DCN gene as major candidate genes for classic EDS, since no causal mutation in these genes was found in a number of patients who tested negative for COL5A1 and COL5A2. 相似文献
102.
Peeters MW Thomis MA Maes HH Loos RJ Claessens AL Vlietinck R Beunen GP 《Behavior genetics》2005,35(5):551-563
Purpose: To determine whether the observed phenotypic stability in explosive strength during adolescence, as measured by inter-age
correlations in vertical jump (VTJ), is mainly caused by genetic and/or environmental factors. Methods: Subjects are from the Leuven Longitudinal Twin Study (LLTS) (n = 105 pairs, equally divided over five zygosity groups). VTJ data were aligned on age at peak height velocity (APHV) to attenuate
the temporal fluctuations in inter-age correlations caused by differences in timing of the adolescent growth spurt. Simplex
models were fitted using structural equation modelling. Results: After aligning the data on APHV, the annual inter-age correlations show a clear simplex structure over a 4 year interval.
The best fitting models included additive genetic and unique environmental sources of variation. Heritability estimates ranged
between 60.8% (CI 37.7%–77.2%) and 87.3% (CI 74.2%–94.0%) for boys and between 76.5% (CI 56.7%–89.0%) and 88.6% (CI 77.8%–94.1%)
for girls. Up to 56.4% and 62.8% of the total variation at the last measurement occasion is explained by additive genetic
factors that already explained a significant amount of variation at previous measurement occasions in boys and girls respectively.
It thus can be concluded that the observed stability of explosive strength during adolescence is mainly caused by a stable
genetic influence in boys and girls. Conclusions: Additive genetic factors seem to be the main cause of the observed phenotypic stability in VTJ performance in boys and girls
during adolescence. 相似文献
103.
Srikantan S. Nagarajan Dominique M. Durand Kai Hsuing-Hsu 《Annals of biomedical engineering》1997,25(1):112-125
We determined the location of excitation for different positions of a round and butterfly coil duringin vitro magnetic stimulation of cut peripheral nerves. We analyzed the conditions under which excitation occurs, either at the termination
or at the peak of the field gradients (first spatial derivative of the electric field). These results were then compared to
predictions about the location of excitation sites from a theoretical model of magnetic stimulation of finite neuronal structures.
Excitation along a straight nerve occurred at terminations when 1) a coil was positioned close to the end of a nerve (at least
one diameter length from the end), 2) a nerve ended in a finite terminating impedance much greater than the axial resistance
of the nerve, 3) the induced electric field was of sufficient magnitude, pointing in a direction away from the axis of a nerve.
Excitation occurred at the negative peak of the field gradients along a nerve when 1) a coil was positioned far away from
the ends of a nerve, 2) there were no geometric or volume conductor inhomogeneities around a nerve, and 3) it was of sufficient
magnitude. Threshold strengths for excitation at terminations were significantly lower than that for field gradient excitation
and comparable to that due to geometric and volume conductor inhomogeneities. 相似文献
104.
105.
Földes-Papp Z Costa JM Demel U Tilz GP Kinjo M Saito K Kii H Takagi T Tamura M Thyberg P Birch-Hirschfeld E 《Experimental and molecular pathology》2004,76(3):212-218
Whole blood samples of known methylene tetrahydrofolate reductase (MTHFR) genotypes from 24 individuals were examined at site C677T. Their amplified DNA products were assessed by two-color fluorescence cross-correlation measurements and agarose gel electrophoresis/capillary gel electrophoresis. DNA subpopulations were identified which were not associated with the proper genotype by primer combinations and cycling conditions called multiplexes. We confirmed that DNA analysis by two-color fluorescence cross-correlation measurements allowed the detection of fluorescence signals specifically associated with the proper genotypes in a mixture of amplified nontarget DNA molecules without DNA sizing. The measurement approach does not require complex, follow-up mathematical analysis and is applicable to any single nucleotide polymorphisms. The simple immunogenetic model showed how the approach works to reveal specific DNA target by preventing detection of nontarget DNA. Under those experimental conditions, a new ultrasensitive, and specific method for clinical immunologists is born. 相似文献
106.
抚触对人工喂养新生儿生长发育的影响 总被引:1,自引:0,他引:1
目的 探讨抚触对人工喂养儿生长发育影响。方法 选择30例人工喂养新生儿进行抚触,并随机选择条件相似的30例人工喂养儿做对照,100d时比较两组婴儿的身长、头围、体质量、日摄奶量、睡眠时间、大便情况。结果 抚触组人工喂养婴儿的体质量、摄奶量较对照明显增加,睡眠时间较对照组长,且较对照组大便正常,较少发生便秘。身高、头围无明显差异。结论 抚触能明显提高人工喂养儿的体质量及摄奶量,促进排泄,改善睡眠。 相似文献
107.
This paper illustrates how a simple geometric model resembling the shape of the chick wing bud at an early growth stage can be mathematically expanded to simulate subsequent growth characteristics of the developing bud. The model was tested against several sets of experimental data and gave an acceptable representation of growth over the range considered. Representing growth patterns in this form enables the determination of differential growth characteristics in different parts of the bud and provides boundary constraints which will play an important part in the eventual evaluation of internal growth mechanisms. 相似文献
108.
D. Dory C. Chopin I. Aimone-Gastin J. L. Gueant L. Guerin J. Sainte-Laudy D. A. Moneret-Vautrin J. Fleurence 《Allergy》1998,53(1):42-50
Allergy to fish is one of the most common food allergies. Gad c 1 is the only fish allergen which has been purified and characterized. Other allergens have been detected by Western blot in cod extracts. We have now improved the Western-blot procedure in order to characterize fish IgE-reactive proteins from extracts prepared under different conditions: pre-rigor mortis and postrigor mortis. EDTA addition or not. and DEAE ion-exchange chromatography. Several IgE-reactive protein bands have been identified over a wide molecular-weight range. In particular, the 104- and 130-kDa IgEreactive protein bands were detected. These new bands may correspond to aggregates, as EDTA increased the relative amount of the 60-, 67-, 104-, and 130-kDa IgE-reactive protein bands in Western blot. All these bands were also detected by an antiparvalbumin monoclonal antibody, specific to the first calcium-binding site. The longer period of storage increased the relative amounts of the 41-, 80-, 104-. and 130-kDa IgE-reactive protein bands. The 18-kDa band was detected only in fish stored for several days. In conclusion, we have described IgE-reactive protein bands over a wide molecular-weight range (12–130 kDa) in Western blot of cod extract, and shown that EDTA and storage conditions may influence the relative distribution of IgE-reactive protein bands. 相似文献
109.
Kinoshita-Kawada M Tang J Xiao R Kaneko S Foskett JK Zhu MX 《Pflügers Archiv : European journal of physiology》2005,450(5):345-354
The transient receptor potential canonical type 5 (TRPC5) channel is a member of the channels that has been implicated in neurite extension and growth cone morphology of hippocampal neurons. Although homomeric TRPC5 channels are activated following stimulation of Gq/11-coupled receptors, the exact mechanism for this activation remains unresolved. Using two-electrode voltage clamp recordings, we show that the activity of TRPC5 channels expressed in Xenopus oocytes is dependent on the presence of Ca2+ at the extracellular as well as the cytoplasmic side of the plasma membrane. TRPC5 was activated by the stimulation of coexpressed M5 muscarinic receptors or by ionomycin. The TRPC5 activity was detectable with the presence of submillimolar levels of extracellular Ca2+, but it was eliminated by the injection of 5 mM 1,2-bis(o-aminophenoxy)ethane-N,N,N,N-tetraacetic acid into the oocytes. Lanthanum could substitute for extracellular Ca2+ to support TRPC5 activity. Coexpression of Ca2+-binding protein 1 (CaBP1), but not calmodulin (CaM), inhibited the TRPC5 activity, without affecting the cell surface expression of TRPC5 proteins. Using in vitro binding assays, we demonstrated direction interactions between CaBP1 and TRPC5. The CaBP1-binding sites at the C terminus of TRPC5 are closely localized, but not identical, to CaM-binding sites. We conclude that TRPC5 is a Ca2+-regulated channel, and its activity is negatively controlled by CaBP1. 相似文献
110.
目的 探讨不同剂量生长激素 (GH)在体外受精 -胚胎移植 (IVF -ET)中 ,对低反应患者超排卵周期的作用。方法 前一IVF周期卵巢低反应的患者 30例 ,她们在前一周期采用促性腺激素释放激素激动剂 (GnRH -a)和促性腺激素(Gn)治疗 ,本周期采用GnRH -a、Gn、GH(两种不同剂量 )治疗 ,比较两种不同剂量生长激素的作用。结果 两种剂量生长激素均可提高受精率 ,增加优质胚胎数 ,提高妊娠率 ,且不同剂量生长激素间的作用无显著差异。结论 生长激素在IVF超排卵中有辅助作用 ,且不同剂量作用无显著差异 相似文献