首诊于眼科的IgG4相关疾病初步研究

目的 对一组首诊于眼科的IgG4相关疾病患者进行总结分析.方法 回顾性病例分析.选取2011年1月～2021年4月解放军总医院第一医学中心神经眼科住院治疗的9例IgG4相关眼病(IgG4-ROD)纳入分析.总结患者临床表现、实验室检查、眼眶磁共振成像(MRI)、病理学检查和治疗情况.结果 9例患者,男性6例、女性3例,...     

佛司可林类化合物对离体豚鼠气管条舒张作用的研究

目的研究佛司可林、异佛司可林、佛司可林G、J、A、I对磷酸组胺(His)、氯化乙酰胆碱(ACh)引起的离体豚鼠气管条收缩作用的影响。方法取豚鼠气管制成螺旋状气管条,用磷酸组胺、氯化乙酰胆碱诱发气管条收缩,按累积剂量法加入系列浓度的佛司可林、异佛司可林、佛司可林G、J、A、I,观察其对气管条收缩反应的舒张作用,记录各累积浓度下的量效曲线,计算解痉百分率。结果异佛司可林、佛司可林均能明显舒张由His、ACh引起的离体豚鼠气管条的收缩(P<0.01)。佛司可林G、J、A、I均能舒张由His引起的离体豚鼠气管条的收缩(P<0.01)。且随着剂量的增加,舒张作用也有所增强。结论佛司可林、异佛司可林、佛司可林G、J、A、I对His或ACh引起的离体豚鼠气管条的收缩均具有舒张作用。     

G蛋白抑制肽GCIP-27对小鼠心肌肥大的影响

目的:探讨G蛋白抑制肽GCIP-27对去甲肾上腺素诱导的小鼠心肌肥大的作用。方法:50只小鼠随机分为对照组、模型组和GCIP-27组,分别给予生理盐水、去甲肾上腺素和GCIP-27低、中、高剂量(10、30、100μg·kg-1)连续15d。之后称量小鼠体质量、心脏重、左心室重,计算心脏指数和左心室指数。采用免疫组化法测定心肌组织的原癌基因c-Fos和细胞外信号调节激酶p-ERK1/2表达。结果:与对照组比较,模型组可明显诱导小鼠产生心肌肥大。与模型组比较,GCIP-27各剂量组能明显降低心脏指数和左心室指数,减少心肌组织中c-Fos和p-ERK1/2的表达量(P<0.05或P<0.01)。结论:GCIP-27可抑制小鼠发生心肌肥大,其机制可能与抑制c-Fos和p-ERK1/2表达有关。     

乳状液膜法提取青霉素非稳态传质数学模型的研究

在“渐进前沿模型”的基础上 ,建立并推导了乳状液膜法提取青霉素的非稳态传质数学模型。实验条件下乳状液膜法提取青霉素的过程属扩散控制 ,其阻力主要来源于膜相。青霉素在膜相中的扩散是提取过程的速控步骤     

Two generation reproduction and teratogenicity studies of feeding cyadox in Wistar rats

To investigate the teratogenic potential and reproductive toxicity of cyadox, a growth promoting agent, Wistar rats (F₀) were fed with diets containing cyadox (0, 50, 150 and 2500 mg/kg) or olaquindox (150 mg/kg), approximately equivalent to cyadox 5, 15, 250 or olaquindox 15 mg/kg b.w./day across two generations. Half of the pregnant rats (F₀, F_1b) were subjected to caesarean section on gestational day 20 for teratogenic examination and the other half produced pups F_1a and F_2a, respectively. At the 250 mg/kg b.w./day cyadox group, body weights of F_1b pregnant rats and F_2a on day 21 after birth decreased; fetal body lengths and tail lengths decreased; the number of fetal resorptions increased significantly; litter weights, number of viable fetuses decreased; number of embryo resorptions increased significantly; number of liveborn F_1a, F_1b and F_2a decreased. No macroscopic or microscopic change of any significance was found in the reproductive organs. Significant increases in the incidence of cervial ribs or lumbar ribs in F_2a pups and significant increases of relative organ weight of testis and epididymis in F_1b were observed at the 250 mg/kg b.w./day cyadox group. The NOAEL for reproduction/development of cyadox for rats was estimated to be 150 mg/kg diet, which was equivalent to approximately 15 mg/kg b.w./day.     

Cyanidin-3-O-glucoside and Peonidin-3-O-glucoside-Rich Fraction of Black Rice Germ and Bran Suppresses Inflammatory Responses from SARS-CoV-2 Spike Glycoprotein S1-Induction In Vitro in A549 Lung Cells and THP-1 Macrophages via Inhibition of the NLRP3 Inflammasome Pathway

Black rice is a functional food that is high in anthocyanin content, primarily C3G and P3G. It possesses nutraceutical properties that exhibit a range of beneficial effects on human health. Currently, the spike glycoprotein S1 subunit of SARS-CoV-2 (SP) has been reported for its contribution to pathological inflammatory responses in targeting lung tissue and innate immune cells during COVID-19 infection and in the long-COVID phenomenon. Our objectives focused on the health benefits of the C3G and P3G-rich fraction of black rice germ and bran (BR extract) on the inhibition of inflammatory responses induced by SP, as well as the inhibition of NF-kB activation and the NLRP3 inflammasome pathway in an in vitro model. In this study, BR extract was identified for its active anthocyanins, C3G and P3G, using the HPLC technique. A549-lung cells and differentiated THP-1 macrophages were treated with BR extract, C3G, or P3G prior to exposure to 100 ng/mL of SP. Their anti-inflammatory properties were then determined. BR extract at concentrations of 12.5–100 μg/mL exhibited anti-inflammation activity for both A549 and THP-1 cells through the significant suppression of NLRP3, IL-1β, and IL-18 inflammatory gene expressions and IL-6, IL-1β, and IL-18 cytokine secretions in a dose-dependent manner (p < 0.05). It was determined that both cell lines, C3G and P3G (at 1.25–10 μg/mL), were compatibly responsible for the significant inhibition of SP-induced inflammatory responses for both gene and protein levels (p < 0.05). With regard to the anti-inflammation mechanism, BR extract, C3G, and P3G could attenuate SP-induced inflammation via counteraction with NF-kB activation and downregulation of the inflammasome-dependent inflammatory pathway proteins (NLRP3, ASC, and capase-1). Overall, the protective effects of anthocyanins obtained from black rice germ and bran can be employed in potentially preventive strategies that use pigmented rice against the long-term sequelae of COVID-19 infection.     

天麻3个不同变型遗传关系的SRAP分析

目的 从分子水平上分析对比天麻的3个不同变型,探索其种内变异,为资源保护和良种选育奠定基础.方法 采用SRAP分子标记分析天麻3个不同变型的遗传关系.结果 筛选出7对SRAP引物,用PCR扩增11批天麻样品,得到145条清晰的带,其中110条具有多态性,多态百分率为75.86％;通过Nei&Li遗传相似系数(GS)计算,11批天麻的种质两两间的相似系数分布在0.36 ～ 0.93;UPGMA聚类分析将乌天麻聚为一类,红天麻与绿天麻聚为一类.结论 天麻的3个不同变型中,绿天麻和红天麻的亲缘关系较近,而乌天麻与前两者的遗传差异较大,亲缘关系较远.     

普鲁卡因青霉素的溶液微粒结晶 Ⅰ.溶解度和超溶解度的测定

采用激光测量装置研究了普鲁卡因青霉素在不同温度、不同浓度、不同ｐＨ值的氯化钠水溶液中的溶解与超溶解特性,为系统研究普鲁卡因青霉素溶液微粒结晶过程提供了一定的热力学基础。     

23G和20G玻璃体切除联合超乳术治疗特发性黄斑前膜合并白内障的疗效比较

目的:对比研究23 G和20 G玻璃体切除手术(pars plana vitrectomy,PPV)联合内界膜剥除、白内障超声乳化人工晶状体植入术治疗合并白内障的特发性黄斑前膜(idiopathic macular epiretinal membrane,IMEM)的临床疗效.方法:采用非随机对照回顾性研究.对合并白内障的IMEM患者45例45眼,分别实施23 G和20 G PPV联合内界膜剥除、白内障超声乳化合并人工晶状体植入术,术后随访90 d.对比术中玻璃体切除时间、剥膜时间、平均超声能量(average ultrasound energy,AVE)和有效超声乳化时间(effective phacoemulsification time,EPT),术前与术后1、7 d的眼压,术前与术后30、90 d最佳矫正视力(best corrected visual acuity,BCVA)和黄斑中心凹视网膜厚度,术后90 d角膜内皮细胞计数.结果:23G组玻璃体切除时间为12.57±1.35min,20G组玻璃体切除时间为17.30±1.19min,两者比较差异有显著统计学意义(t=-12.488,P<0.01).两组剥膜时间比较差异无统计学意义(t=-0.68,P=0.500).术后1d,23G组的眼压略低于20 G组,差异有显著统计学意义(t=-2.345,P=0.024).术后两组患者的BCVA和黄斑中心凹视网膜厚度均较术前明显改善.两组患者的眼压、BCVA和黄斑中心凹视网膜厚度组间差异均无统计学意义(F=0.465、1.895、0.689,P=0.499、0.176、0.411).两组患者的眼压、BCVA和黄斑中心凹视网膜厚度不同时间点差异均有统计学意义(F=291.245、103.06、665.402,P<0.01).23G和20G不同手术方式与不同时间点的眼压有交互作用(F=13.245,P<0.01),而不同手术方式与不同时间点的BCVA和黄斑视网膜厚度无交互作用(F=1.212、2.293,P=0.283、0.129).两组的平均超声能量和有效超声时间差异无统计学意义(t=-1.186、-0.737,P=0.242、0.465).术后90d,23G组患者的角膜内皮细胞计数略高于20G组,差异有统计学意义(t=2.049,P=0.048).结论:23 G PPV联合白内障超声乳化人工晶状体植入术治疗合并白内障的IMEM效果确切.和20 G相比,23 G PPV手术时间短,对角膜内皮细胞损伤小,但存在术中隐性切口漏造成术后一过性低眼压的可能.     

从青霉素G生产7-氨基脱乙酰氧基头孢烷酸

综述了以青霉素Ｇ钾为原料经氧化,酯化,扩环,水解,脱苯乙酰基等反应制备了７－氨基脱乙酰氧基头孢烷酸的合成工艺。