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61.
目的观察联合进路鼓室成形术治疗慢性化脓性中耳炎手术疗效。方法应用联合进路鼓室成形术治疗慢性化脓性中耳炎42例共48耳,其中Ⅰ期手术者24例28耳,分期手术者18例20耳,术后随访3—5年。结果Ⅰ期及分期手术后鼓膜愈合率分别为75%和90%,胆脂瘤复发率为2143%及5%,语言频率(05、1、2kHz)纯音听阈均值气骨导差小于20dBHL者分别为13耳(4643%)和15耳(75%),其中儿童患者分期手术术后胆脂瘤无一例复发。结论联合进路鼓室成形术分期手术治疗慢行化脓性中耳炎远期效果显著,儿童患者行该术时以分期手术为宜。 相似文献
62.
I. P. Levshina L. V. Nozdracheva N. N. Shuikin 《Bulletin of experimental biology and medicine》1997,124(2):741-743
Rats with catatonic freezing are characterized by low motor and explorative activity in the open field test and specific pattern
of external respiration, so-called strenuous respiration, which is accompanied by a higher activity of the respiratory enzymes
succinate dehydrogenase and NADH dehydrogenase in the hippocampus and motor cortex. No dependencies on the brain structure
and animal age are noted.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 124, No. 8, pp. 138–140, August, 1997 相似文献
63.
目的探讨SAP并发深部真菌感染的易感因素以及防治的方法。方法分析1998年9月至2004年10月收治的81例SAP患者资料,其中并发深部真菌感染的21例作为真菌感染组,其余60例作为非感染组,比较两组患者的诊治方法与措施,分析真菌感染的危险因素。结果经糖皮质激素应用、腹腔灌洗、胃肠减压等一系列诊治措施,真菌感染组19例患者病愈出院,2例死亡。两组在糖皮质激素应用、中心静脉置管、全胃肠外营养、空肠营养、呼吸机支持、腹腔灌洗和囊肿穿刺等方面差异显著(P〈0.05)。结论糖尿病、老年、呼吸机支持和重症监护客观因素等为真菌感染的危险因素。合理应用抗生素和预防性应用抗真菌药物可以减少感染机会,提高SAP的救治存活率。 相似文献
64.
E. Faber D. Riegrová M. Jarošová J. Hubácek P. Slezák Z. Pikalová P. Hamal K. Indrák 《Annals of hematology》1996,73(4):195-198
The case report of a 61 year-old man with AML M2 FAB, t(1; 13; 14) and zygomycotic mesenterial thromboangiitis is presented.
Two induction cycles of chemotherapy were administered due to primary drug resistance. They were complicated by pneumonia,
colonic pseudo-obstruction and perforation with peritonitis. The patient died on the 40th day of therapy, 4 days after undergoing
palliative surgery. Zygomycotic thromboangiitis, which very probably contributed to the intestinal perforation, was confirmed
morphologically at necropsy. The novel complex chromosomal translocation t(1; 13; 14) (q31; q32; q24) and the problems connected
with the diagnosis of invasive fungal infections are discussed.
Received: 26 January 1996 / Accepted: 12 June 1996 相似文献
65.
Summary The glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of Podospora anserina has been isolated from a genomic library by heterologous hybridization with the corresponding gene of Curvularia lunata. The coding region consists of 1014 nucleotides and is interrupted by a single intron. The amino-acid sequence encoded by the gpd gene shows a high degree of sequence identity with the corresponding gene products of various fungi. Multiple alignments of all fungal GPD sequences so far available resulted in the construction of a phylogenetic tree. The evolutionary relationships of the various fungi belonging to different taxa will be discussed on the basis of these data. Sequence analysis of 1.9 kbp of the 5 non-coding region revealed the presence of typical fungal promoter elements. Utilizing different parts of the 5 regulatory sequence of the Podospora gpd gene, expression vectors containing a dominant selectable marker gene (hygromycin B phosphotransferase) have been constructed for the transformation of P. anserina protoplasts. The use of these homologous gpd regulatory sequences resulted in a significant increase in transformation efficiencies compared to those obtained with vectors in which the selectable marker gene is under the control of the corresponding heterologous promoter of Aspergillus nidulans. 相似文献
66.
Alberto Utrero-Rico Javier Ruiz-Hornillos Cecilia González-Cuadrado Claudia Geraldine Rita Berta Almoguera Pablo Minguez Antonio Herrero-González Mario Fernández-Ruiz Octavio Carretero Juan Carlos Taracido-Fernández Rosario López-Rodriguez Marta Corton José María Aguado Luisa María Villar Carmen Ayuso-García Estela Paz-Artal Rocio Laguna-Goya 《The Journal of allergy and clinical immunology》2021,147(5):1652-1661.e1
67.
Cloning of the nitrate reductase gene of Stagonospora (Septoria) nodorum and its use as a selectable marker for targeted transformation 总被引:1,自引:0,他引:1
The nitrate reductase gene (NIA1) of the phytopathogenic fungus Stagonospora (Septoria) nodorum has been cloned from a cosmid library by homologous hybridisation with a PCR-generated probe. A 6.7-kb fragment carrying
the NIA1 gene was subcloned and partially characterised by restriction mapping. Sequencing of the gene indicated a high degree of
homology, both at the nucleotide and amino-acid levels, with nitrate reductase genes of other filamentous fungi. Furthermore,
consensus regulatory signals thought to be involved in the control of nitrogen metabolism are present in the 5′ flanking region.
The cloned NIA1 gene has been used to develop a gene-transfer system based on nitrate assimilation. Stable nia1 mutants of S. nodorum defective in nitrate reductase were isolated by virtue of their resistance to chlorate. These were transformed back to nitrate
utilisation with the wild-type S. nodorum NIA1 gene. Southern analyses revealed that transformation occurred as a result of the integration of transforming DNA into the
fungal genome; in all cases examined, integration was targeted to the homologous sequence.
Received: 30 March / 9 June 1998 相似文献
68.
Lymphoid and non-lymphoid cells in the adenoid of children with otitis media with effusion: a comparative study 总被引:1,自引:0,他引:1
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E B van Nieuwkerk C J de Wolf E W Kamperdijk S van der Baan 《Clinical and experimental immunology》1990,79(2):233-239
We characterized on immuno- and enzymecytochemical level the lymphoid and non-lymphoid cells in the adenoid of children with upper respiratory tract infections (URI) and otitis media with effusion (OME) and compared these with the adenoid of children with URI without OME and with the adenoid of 'healthy' children and adults. Besides macrophages and dendritic cells we also showed the presence of MHC class II positive, ciliated, epithelial cells. These non-lymphoid cells were present in all adenoids. However, their number was less than 1% of all cells. We found no difference in lymphocyte subsets from children with URI + OME compared with those from children with URI alone. These two groups showed a significant decrease of CD8-positive (suppressor/cytotoxic) cells and a slight increase in CD22-positive B cells in comparison to 'healthy' children. No difference was found in percentages of CD4-positive (helper/inducer) cells. The localization of the lymphoid subsets in adenoids of children with URI and/or OME did not differ from those of 'healthy' children and adults. 相似文献
69.
目的:为设计阴部外动脉阴茎皮瓣转位尿道成形术提供依据。方法:30侧经动脉内灌注红色乳胶的成人尸体,解剖观测阴部外动脉起始、行程;着重阴部外动脉阴茎支在阴茎的走行、分支分布。结果:阴部外动脉始于股动脉,外径1.8±0.4mm,伴行静脉1-2支,汇入大隐静脉。阴茎支可视为本干的延续,经耻骨结节两侧靠近阴茎,分别经2(10)点、3(9)点和1(11)点进入阴茎,多数分出背侧支、腹侧支分布阴茎皮肤。外径0.8±0.2mm。结论:阴茎皮肤血管恒定,以阴茎支为蒂,可在阴茎外侧或背外侧、腹外侧设计皮瓣,用于尿道成形术。术式已在临床应用,效果满意。 相似文献
70.
DC-SIGN mediates the binding of Aspergillus fumigatus and keratinophylic fungi by human dendritic cells 总被引:2,自引:0,他引:2
Human contact with fungi does not usually lead to pathological consequences, as the immune system manages to defeat the invader pathogens. Nevertheless, under immune suppression, fungi overcome immune defenses and cause diseases that range from nonserious colonizations of keratinizated tissue (Dermatophytosis) to life threatening disseminated infections (Aspergillosis). Host defenses against fungi rely on innate and adaptative responses, with dendritic cell (DC) and macrophage surface receptors having a major role in the recognition of fungal pathogens and in the orchestration of an effective immune response. DC-SIGN is a C-type lectin involved in the recognition of bacterial, viral and parasitic pathogens, as well as in interactions between cells of the immune system. Its expression is restricted to DCs and subsets of macrophages. Here we show that DC-SIGN mediates the binding and capture of Aspergillus fumigatus and keratinophylic fungi, including Chrysosporium tropicum, by human DCs, describe the requirements of these interactions and discuss their potential involvement in the onset and persistence of pulmonary fungal infections. 相似文献