Influence of prednisolone on the secretion of mucin from the HT29‐MTX cell line

Influence of prednisolone on the secretion of mucin from the HT29‐MTX cell line Glucocorticoids have been used in the treatment of otitis media with effusion with promising but inconsistent results. The HT29‐MTX cell line is a completely differentiated and almost exclusively mucus‐secreting goblet cell line. To assess the potential of steroids in suppressing mucin secretion, we have studied the response of this cell culture to prednisolone. Confluent cell cultures were trypsinized, subcultured in six‐well plates and incubated with five doses of prednisolone from 10^?3 M to 10^?11 M and over a varying time course from 6 to 36 h. Analysis was performed using a monoclonal mouse antibody to human gastric mucin by dot‐blot ELISA. Prednisolone caused a consistent reduction in mucin production from this cell line. Increasing concentrations of prednisolone resulted in increasing suppression of MUC5AC secretion. There is a dose‐dependent suppression of mucin secretion by prednisolone, with a maximum effect of 21% over control seen at the highest steroid concentration used.     

中药百部酊治疗阴虱病50例

用中药百部于医用酒精中浸泡一周，制成百部酊，局部涂探治疗阴虱病，共治50例，9天为1个疗程，全部治愈。     

耳源性脑脓肿105例临床分析

目的 本文的目的为探讨耳源性脑脓肿的发生、诊断和治疗。方法 对1952～2002年治疗的105例耳源性脑脓肿患者(颞叶66例,小脑38例),对疾病的发生、诊断和治疗方式进行回顾性分析,特别是手术所见、诊断方法、治疗方法和结果。结果本组中发病年龄主要在10～30岁,10岁以内和更高的年龄组较少见。头痛(99.05％)、淡漠和昏迷(91.43％)是较常见的症状。105例中神经系统检查异常者78例。23例经CT诊断。经乳突腔穿刺抽脓者63例,外科钻颅穿刺抽脓26例,脓肿切除17例。死亡15例,其中66例颞叶脓肿中11例死亡,38例小脑脓肿中3例,脓肿部位不明者1例。结论 正确掌握经乳突腔穿刺抽脓的适应证对耳源性脑脓肿的治疗十分重要。颅骨钻孔穿刺引流和脑室引流是脑疝的有效的紧急处理措施。自CT用于耳源性脑脓肿的诊断和随访以来,死亡率明显下降。     

祛腐生肌散治疗难愈性切口感染临床观察

目的　观察中药祛腐生肌散治疗难愈性切口感染的效果。方法　选用蜈蚣、冰片等 5味中药研末制成散剂 ,换药时撒薄层于无菌凡士林油纱布上 ,敷或塞于伤口。结果　 1个月内治愈 18例 ,2个月内治愈 10例 ,3个月内治愈 8例 ,无效 1例。结论　中药祛腐生肌散具有活血止痛、祛腐生肌之功效 ,治疗难愈性切口感染疗效显著。     

外用湿敷汤治疗皮损严重的各种皮肤病178例

目的观察外用湿敷汤治疗皮损严重的皮肤病的疗效.方法对178例病历进行临床总结及分析.结果有效率98.88%,治愈率84.83%.结论外用湿敷汤治疗皮损严重的各种皮肤病疗效好,值得推广.     

急性化脓性中耳炎豚鼠耳蜗形态和功能改变

对3只因听泡置管导致急性化脓性中耳炎发作的豚鼠进行了脑干诱发电位（ABR）测试和耳蜗Corti氏器扫描电镜观察。发现3只豚鼠患耳Corti氏器均有程度不等损害，ABR阈分别提高45、60和30dB。提承急性中耳炎可引起内耳形态和功能变化。文中对引起损害的机制进行了讨论。     

Survival in cancer of the cervix: treatment in a population-based cancer registry in a developing country (Bangalore, India)

A survival analysis of treated cases of cervix cancer that were registered in the Bangalore (India) Population Based Cancer Registry between 1 January 1987 and 31 December 1989 was performed. Information on vital status of patients was obtained principally through follow-up visits to homes of patients. Follow-up information was available for 860 (92.7 percent) of 928 registered cases. Of the 860 cases, information on treatment was available for 559 patients, on whom the analysis of treatment outcome was performed. The overall five-year observed survival (5YS) was 41.1 percent with a relative survival of 46.3 percent. The 5YS was significantly (P = 0.01) influenced by clinical stage and by addition of brachytherapy (BT) to external radiotherapy (EXT) (5YS = 60.1 percent cf 27.4 percent, P = < 0.001). In 343 patients who received EXT only, comparatively better survival was seen in the group who had received between 4,800 to 5,999 centigray (cGy) (5YS = 36.1 percent) when compared with those who received less than 3,000 and 3,000 to 4,799 cGy (5YS = 16.7 percent and 24.9 percent, respectively). Doses of EXT higher than 5,999 cGy (in patients who were not suitable for BT) did not have any benefit in the 5YS (27.4 percent). The study has generated a specific hypothesis about possible needless excess dose of external radiotherapy.     

Steno-Occlusive Changes in the External Carotid System in Moyamoya Disease

To evaluate the steno-occlusive changes in the external carotid system in moyamoya disease, cerebral angiograms of 39 moyamoya patients were retrospectively reviewed. There were 26 females and 13 males, age ranged from 4 to 62 years with a mean of 26 years. Initial symptoms were ischaemia in 27 patients, haemorrhage in 9, and none in 3. Stenosis, occlusion, and dilatation in the external carotid system were analysed angiographically. No stenosis or occlusion of the superficial temporal artery, middle meningeal artery, or occipital artery was observed in either preoperative or postoperative follow-up angiograms in any patients. Steno-occlusive changes do not occur in the external carotid system, but are confined in the internal carotid system in moyamoya disease.     

盐酸氨溴索治疗分泌性中耳炎疗效分析

目的总结促表面活性物质合成剂盐酸氨溴索治疗分泌性中耳炎的临床研究结果,判断其临床疗效及安全性.方法将100例分泌性中耳炎患者分为治疗组和对照组各50例,两组同时给予常规治疗,其中治疗组加用盐酸氨溴索治疗,对比观察两组的治疗效果并作统计学处理.结果治疗2周后,治疗组的有效率明显高于对照组(P<0.05),且无不良反应.结论盐酸氨溴索治疗分泌性中耳炎安全有效.     

耳炎差异球菌与成人分泌性中耳炎的相关性研究

目的探讨耳炎差异球菌与成人分泌性中耳炎的关系。方法采用多重PCR的方法，检测39例(42耳)成年分泌性中耳炎患者的中耳积液中耳炎差异球菌以及三种常见细菌[肺炎链球菌(S．pneumoniae)、流感嗜血杆菌(H．influenzae)和卡他莫拉杆菌(M．catarrhalis)]的DNA，就细菌DNA存在与成人分泌性中耳炎患者上呼吸道感染(简称上感)史、病程、积液性质和抗生素服用史之间的关系进行分析。结果收集到的42份中耳积液标本中有5份(11．9％)检测到有耳炎差异球菌DNA存在。急性分泌性中耳炎者有1例(3．6％)测得耳炎差异球菌DNA存在，慢性者则有4例(28．6％)，两者差异有显著性意义。浆液性中耳积液有3例(13．0％)测得耳炎差异球菌DNA存在，粘液性中耳积液则有2例(10．5％)为阳性，两者差异有显著性意义。抗生素使用史对耳炎差异球菌DNA检出率的影响无统计学意义。结论细菌参与部分成人分泌性中耳炎的发病，耳炎差异球菌可能是其致病菌之一；且慢性分泌性中耳炎中耳积液的持续存在可能与耳炎差异球菌感染有关。