Effects of isoflurane and halothane on the calcium ion-tension curve in rat myocardium

In order to clarify the interaction of volatile anesthetics and extracellular calcium ion on the myocardial contraction, effects of both isoflurane (1.0%) and halothane (0.5%) on the extracellular calcium ion concentration ([Ca²⁺]_O)-tension curve were studied. Increasing [Ca²⁺]_O enhanced the myocardial contraction response, and the maximal response was obtained at [Ca²⁺]_O of 3.0mM. Halothane depressed the maximal value of the tension development in response to increasing [Ca²⁺]_O, while isoflurane did not (P 0.01). The probit response of the developed tension to the changes in [Ca²⁺]_O indicated that isoflurane increased the median effective concentration (EC₅₀) of [Ca²⁺]_O significantly from 0.484 ± 0.051 (mean ± SEM) to 0.870 ± 0.056mM (P = 0.001), but halothane did not (P = 0.018). Therefore, 1.0% isoflurane was concluded to move the [Ca²⁺]_O-tension curve to the right, while a downwards shift occurred with 0.5% halothane.(Saeki S, Hirakawa M, Shimosato S: Effects of Isoflurane and Halothane on the Calcium Ion-tension Curve in Rat Myocardium. J Anesth 6: 172–175, 1992)     

Aggregated plasmalemmal vesicles and microvilli in human astrocytoma

Summary Plasmalemmal vesicles and microvilli or filopodia in human astrocytoma were examined with thin-section and freeze-fracture techniques. The plasmalemmal vesicles, although sparsely scattered in distribution, were often aggregated in a curvilinear, semicircular, or circular fashion, and the involved plasma membrane was often protruded externally like a mound.Microvilli or filopodia were cross-fractured when fracture travelled within the plasma membrane resulting in a fracture face interrupted by numerous holes of cytoplasms. The microvilli were distributed in a concentrated manner on the free suface of the cell and often closely oriented in a linear, curvilinear, or circular fashion. They varied in size, form, and length. When they were arisen from a common hillock of the plasma membrane, its fracture face was elongated, up to 0.8 in length. Fenestrae were occasionally visible in attenuated cytoplasm which surrounded peripheral vacuole in the cell.     

局部炎症反应与无细胞异种真皮基质移植物的降解吸收关系

目的 探讨不同方法制作的无细胞异种真皮基质(Xeno-ADM)移植物降解吸收与炎症反应之间的关系。方法 猪和兔断层中厚皮片经Trypsin和TritonX-100等脱细胞,分别制成Xe-no-ADM和异体ADM(Allo-ADM),再按不同的预处理因素进行分组:(1)戊二醛交联的Xeno-ADM(A)组;(2)网状交联的Xeno-ADM(B)组;(3)未交联的:Xeno-ADM(C)组,预先被Xeno-ADM蛋白致敏的兔皮下植入;(4)交联的Xeno-ADM(D)组;(5)Allo-ADM(E)组。每组80块ADM分别埋植于兔耳和背部皮下。结果在背部的移植物较耳部炎性反应和降解吸收明显,至术后32wk时,E组以外的其它组移植物解剖学和组织学结构消失,D组和A组炎症反应滞后,可见较多的异物巨细胞,各组ADM炎症反应与降解/吸收的程度依次为:C>B>D>A>E(P相似文献   

EFFECT OF ELECTROACUPUNCTURE OF SCALP-POINTS ON ABNORMAL DISCHARGES OF NEURONSAROUND THE CEREBRAL HEMORRHAGE FOCUS IN THE RAT

Thereexistdifferentdegreesofpathophysi ologicchangesinthetissuesaroundhemorrhagefocusafteracutecerebralhemorrhage[1 ] .Cere bralhemorrhageoftenleadstodysfunctionoftheneuronsinthebrain ,manifestingabnormalelec tricalactivity .Followingacutecerebralhemor rhage,duetotheoppressionofthehematomaandthetoxiceffectofthebloodcontentsaswellaschangesofthemicro environmentaroundthehemorrhagefocus,theelectricalactivityofthecerebralneuronsissuppressedatdifferentde grees.Electroacupuncture (EA)therapyworkell…     

细胞外基质金属蛋白酶诱导因子和基质金属蛋白酶-2在喉癌中的表达

目的　了解细胞外基质金属蛋白酶诱导因子 (EMMPRIN )和基质金属蛋白酶 2 (MMP 2 )在喉癌的表达情况 ,探讨两者表达的关系以及与喉癌浸润和转移的关系。方法　采用免疫组织化学S P法对 47例喉癌和 2 2例正常喉组织中的EMMPRIN和MMP 2的表达情况进行检测。结果　EMMPRIN和MMP 2在喉癌的阳性表达率 (分别为 87.2 %和 91.5 % )明显高于正常喉组织分别为 ( 9.1%和 18.2 % ) ,EMMPRIN和MMP 2的强阳性表达与喉癌的病理分级、临床分期和淋巴结转移情况有关 ;EMM PRIN和MMP 2的表达一致率为 91.5 % ,Kappa值为 0 .614 ,两者表达具有明显的相关性。 结论　EMMPRIN和MMP 2在喉癌有阳性表达 ,表达与喉癌的临床分期和淋巴结转移有关 ,EMMPRIN与MMP 2的产生有密切的关系     

表皮生长因子受体和细胞外信号调节激酶的表达与舌鳞状细胞癌转移关系的观察

目的　了解表皮生长因子受体 (EGFR )和细胞外信号调节激酶 (ERK )在舌鳞状细胞癌中的表达 ,探讨EGFR和ERK与肿瘤增殖、转移的关系。方法　应用免疫组织化学SABC方法 ,分别对 90例舌鳞状细胞癌术后组织标本的EGFR、活化ERK 1/2和Ki 67进行检测 ,并对各因子间的表达关系进行统计分析。结果　在 90例舌鳞状细胞癌组织中 ,2 6例证实有颈部淋巴结转移 ,EGFR、活化ERK 1/2及Ki 67的阳性表达率分别为 93 .3 3 % ( 84/90 )、10 0 .0 0 % ( 90 /90 )和 10 0 .0 0 % ( 90 /90 ) ,阳性细胞分布和反应强度均以肿瘤深部边界区域最为明显 ,统计分析显示EGFR、活化ERK 1/2与肿瘤细胞增殖和肿瘤转移之间呈明显的正相关 (P <0 .0 1)。结论　EGFR、活化ERK 1/2在舌鳞状细胞癌细胞中多呈现过度表达与肿瘤的增殖、转移密切相关     

直肠癌中细胞外信号调节激酶和微血管计数的关系探讨

目的:探讨细胞外信号调节激酶ERK-1、ERK-2在直肠癌中的蛋白表达水平和微血管计数的关系及其在直肠癌发生发展中的意义.方法:应用蛋白印迹法检测62例直肠癌及其邻近正常直肠粘膜中ERK-1和ERK-2蛋白的表达情况;Ⅷ因子相关抗原抗体免疫组化法测定微血管计数.结果:直肠癌中ERK-1、ERK-2的蛋白表达水平及微血管计数明显高于正常直肠粘膜;微血管计数较高的直肠癌其ERK-1、ERK-2的表达水平也明显增高.结论:ERK-1、ERK-2在直肠癌中呈现高表达,刺激血管内皮生长因子的增高,加速肿瘤微血管的生成,从而促进直肠癌的发生发展.     

nm23-H1基因对人肺癌细胞中细胞外信号调节激酶活性的影响

目的探讨肿瘤转移抑制基因nm23-H1对人高转移大细胞肺癌细胞株L9981中细胞外信号调节激酶ERK1/2活性的影响.方法应用特异性识别总ERK1/2(p44/42 MAP kinase)和双磷酸化ERK1/2(phospho-p44/42 MAP kinase)的抗体及蛋白印迹法(Western blot),检测L9981(缺失nm23-H1基因的原代肺癌细胞株)、L9981-nm23-H1(转染了nm23-H1基因的L9981细胞株)、L9981-PLXSN(转染了空载体的L9981细胞株)中总ERK1/2和磷酸化ERK1/2的水平.磷酸化ERK1/2活性应用非放射性免疫沉淀和Western blot法以及p44/42 MAP kinase分析试剂盒予以检测.结果 L9981-nm23-H1细胞株中磷酸化ERK1/2的水平,以及ERK1/2活性均显著低于L9981细胞株和L9981-PLXSN细胞株(P<0.01),L9981和L9981-PLXSN细胞株间磷酸化ERK1/2水平和ERK1/2活性比较均无显著性差异(P＞0.05).三个细胞株间总ERK1/2水平比较均无显著性差异(P>0.05).结论 nm23-H1基因可明显靶向地抑制人高转移肺癌细胞株L9981中ERK1/2的转录表达和ERK1/2的活性.推测nm23-H1基因的作用机制可能与其抑制了MAPK/ERK信号传导通路有关.     

榄香烯对Lewis肺癌小鼠基底膜及细胞外间质影响的实验研究

目的：研究基底膜及细胞外基质与肺癌浸润和转移的相互关系，以及中药榄香烯的抗癌作用机理方法：以C57BL／6近交系小鼠建立Lewis肺癌模型，将40只小鼠分为正常组、模型组、CTX组及榄香烯组．14天后以放免法及SABC免疫组织化学方法，分别检测各组小鼠血清HA、Col Ⅳ及移植瘤组织LN、FN。结果：榄香烯能降低血清HA、Col Ⅳ含量；维持FN在瘤细胞周围基底膜及细胞外基质中的线状表达，降低肿瘤细胞LN表达：与模型组及CTX组比较有显著差别。结论：中药榄香烯与化疗药CTX抗肿瘤作用机理不同．其抑制肺癌转移的部分作用机制是通过保护基底膜和细胞外基质屏障以及降低肿瘤细胞中细胞外基质成分表达而实现的。     

Simultaneous detection of tyrosine hydroxylase-immunoreactivity and vasopressin mRNA in neurons of the human paraventricular and supraoptic nucleus

The extracellular signal-regulated protein kinase-1 and -2 (ERK1 and ERK2), also referred to as the p44/42 mitogen-activated protein kinase (p44/42 MAP kinase), plays an essential role in neuronal signal transduction, but its function involved in nociceptive response has not been deeply studied yet. Here we report immunohistochemical evidence that p44/42 MAPK might be critical in nociceptive response. We found that after formalin was injected into the perioral skin of the upper lip of mice, the number of activated p44/42 MAPK-like immunoreactive neurons was significantly increased in the laminae I and II of the caudal subnucleus of the trigeminal spinal nucleus (Sp5C). The positive neurons and fibers were mostly concentrated in the middle portion of Sp5C dorsoventrally, where the afferent fibers innervating the skin of the upper lip are terminated. The reactive products were localized in perikarya, dendrites, nuclei, and diffusely in the neuropil. The present result suggests that p44/42 MAPK may be important in the transmission and modulation of noxious information in Sp5C.