蜂花粉醇提物对小鼠体外生成过氧化脂质的影响

组织器官的衰老与过氧化脂质的生成密切相关，本实验证明蜂花粉醇提物有明显的对抗小鼠肝、肾、脑组织在体外生成过氧化脂质的作用，证明蜂花粉具有延缓衰老的作用。     

Five‐grass pollen 300IR SLIT tablets: efficacy and safety in children and adolescents

Halken S, Agertoft L, Seidenberg J, Bauer C‐P, Payot F, Martin‐Muñoz MF, Bartkowiak‐Emeryk M, Vereda A, Jean‐Alphonse S, Melac M, Le Gall M, Wahn U. Five‐grass pollen 300IR SLIT tablets: efficacy and safety in children and adolescents.
Pediatr Allergy Immunol 2010: 21: 970–976.
© 2010 John Wiley & Sons A/S The efficacy and safety of five‐grass pollen 300IR sublingual immunotherapy (SLIT) tablets (Stallergènes SA, France) have previously been demonstrated in paediatric patients. This report presents additional data concerning efficacy at pollen peak, efficacy and safety according to age, nasal and ocular symptoms, use of rescue medication, satisfaction with treatment and compliance. Children (5–11 yr) and adolescents (12–17 yr) with grass pollen–allergic rhinoconjunctivitis were included in a multinational, randomized, double‐blind, placebo‐controlled study and received either a 300IR five‐grass pollen tablet or placebo daily in a pre‐ (4 months) and co‐seasonal protocol. The severity of six symptoms (sneezing, rhinorrhoea, nasal congestion, nasal and ocular pruritis, and tearing) was scored, and rescue medication use was recorded daily during the pollen season. Patient satisfaction was recorded at the season end. A total of 161 children and 117 adolescents were evaluated (n = 267). 300IR SLIT was effective over the whole season (p = 0.0010) and at the pollen peak (p = 0.0009). The adjusted mean difference between 300IR and placebo groups was significant for both nasal (p = 0.0183) and ocular (p < 0.0001) symptoms. Rescue medication use was statistically lower in the SLIT group during the pollen season and at the pollen peak (both p < 0.05). More patients in the SLIT group were satisfied with their treatment compared to placebo (83.2% vs. 68.1%, p = 0.0030), and compliance was high (SLIT 93.9% of patients were compliant, placebo 94.8% of patients were compliant). SLIT was well tolerated by children and adolescents. 300IR five‐grass pollen tablets are effective and safe during the pollen season and at the pollen peak in children and adolescents with grass pollen rhinoconjunctivitis.     

椰子花粉过敏原的分离、鉴定与纯化

目的 对椰子花粉的变应原组分进行初步的分离、鉴定及纯化.方法 提取椰子花粉粗提液,用十二烷基硫酸钠.聚丙烯酰胺凝胶电泳(sDS-PAGE)分离椰子花粉的蛋白质组分并测定其相对分子质量,采用免疫印迹法鉴定其变应原成分,并通过离子交换层析对椰子花粉变应原进行初步分离纯化,免疫印迹进行检测.结果 SDS-PAGE显示椰子花粉粗提液有10条蛋白带,其中相对分子质量(肘,)为60 000、50 000、35 000、28 000、19 000、16 000和14 000的蛋白可与椰子花粉过敏性病人血清IgE结合,且M,50 000、16 000和14 000为主要变应原;离子交换层析结果显示主要过敏原成分主要分布在V峰中.结论 对椰子花粉变应原进行了初步的分离、鉴定和纯化,为临床椰子花粉变态反应疾病的诊断和治疗奠定了基础.     

油菜、蚕豆、柿子、梅花、紫云英和玉米等六种蜂花粉的游离氨基酸分析

<正> 蜂花粉是一种天然营养佳品,尤其是其中富含优质蛋白质。对蜂花粉蛋白质水解后氨基酸的分析,国内外报道较多。但花粉中能被人类直接吸收的游离氨基酸的分析,国内外报道甚少。本文旨在采用一种快速、经济、简便和灵敏度可达3×10~(-12)M的DANS-Cl荧光反应、聚酰胺薄膜层析方法。分析并制作了油菜等六种蜂花粉中游离氨基酸的含量及荧光层     

Micro‐arrayed wheat seed and grass pollen allergens for component‐resolved diagnosis

Background: Wheat is a potent allergen source and can cause baker’s asthma, food and pollen allergy. The aim of the study was to develop an allergen micro‐array for differential diagnosis of baker’s asthma, wheat‐induced food allergy and grass pollen allergy. Methods: We analysed the immunoglobulin‐E reactivity profiles of patients suffering from baker’s asthma, wheat‐induced food allergy and grass pollen allergy to micro‐arrayed recombinant wheat flour allergens and grass pollen allergens and compared these results with clinical results and diagnostic tests based on crude wheat flour, wheat pollen and grass pollen allergen extracts. Results: We identified recombinant wheat flour allergens, which are specifically recognized by patients suffering from baker’s asthma, but not from patients with food allergy to wheat or pollen allergy. rPhl p 1 and rPhl p 5 were identified as marker allergens specific for grass pollen allergy. They can be used to replace grass pollen extracts for allergy diagnosis and to identify grass pollen allergic patients among patients suffering from baker’s asthma and wheat‐induced food allergy. Profilin was identified as a cross‐reactive allergen recognized by patients suffering from baker’s asthma, food and pollen allergy. Conclusions: Our results indicate that it will be possible to design serological tests based on micro‐arrayed recombinant wheat seed and grass pollen allergens for the discrimination of baker’s asthma, wheat‐induced food allergy and grass pollen allergy.     

Allergy History Does Not Predict Skin Test Reactivity in Asthmatic Children

We prospectively assessed how well patient report of allergy to cat, dust mite, and grass predicted the results of skin prick testing to those allergens in 95 asthmatic children. Children between 4 and 18 years old with physician documented asthma provided a detailed standardized allergy history and then underwent skin prick testing. The children were categorized by asthma severity. The diagnostic accuracy, which was the primary outcome measure, as well as sensitivity, specificity, and positive and negative predicted values were calculated for allergy history with regards to skin test reactivity. The diagnostic accuracy of allergy history in identifying skin test reactivity was 65%, 50%, and 56% for cat, dust mite, and grass, respectively. Asthma severity did not affect the diagnostic accuracy. Allergy history was a poor predictor of skin test reactivity in this group of asthmatic children.     

IgE cross-reactivity between birch pollen, mugwort pollen and celery is due to at least three distinct cross-reacting allergens: immunoblot investigation of the birch-mugwort-celery syndrome

Background Allergy to celery is often associated with sensitization to birch and/or mugwort pollen. Objective and methods In a multi-centre study, sera from 23 patients suffering from type I allergy to celery and 15 patients with positive celery RAST but wo clinical sensitization were compared. To examine whether cross-reactivity between celery and mugwort pollen iticludes cross-sensitization to birch pollen allergens, we determined cross-reacting structures in birch pollen, mugwort pollen and celery by means of immunoblotting. Inhibition studies were performed by preincubation of sera with extracts of birch pollen, mugwort pollen, and celery. Results We identified three groups of proteins—homologues of Bet v I and birch profilin (Bet v 2) as well asa group of proteins with a molecular range of 46 to 60 kD—displaying IgE-cross-reactivity, which were shared by birch pollen and celery. Two of these groups of allergens (profilin and the 46 to 60 kD proteins) were also present in mugwort pollen. In this paper we demonstrate that most cross-reacting allergens present in mugwort pollen and celery can also be detected in birch pollen extract. Conclusion Therefore we propose, from a serological point of view, to extend the mugwort-celery syndrome to the birch-mugwort-celery syndrome.     

四川省成都市城区气传花粉监测

目的探索四川省成都市城区气传花粉的种类、数量及飘散规律。方法应用重力沉降法于2018年3月1日至2019年2月28日在四川省成都市城区进行为期1年的连续每日气传花粉曝片。结果共收集到花粉139520粒/1000 mm 2,能鉴定的花粉总计隶属36科、31属、2个种。其中构属花粉含量最高,达81754粒/1000 mm 2,占全年花粉总量的58.60%,其播粉期为3~4月,高峰期为3月下半月至4月上半月;其次是柏科花粉,达27518粒/1000 mm 2,占全年花粉总量的19.72%,其播粉期为1~3月,高峰期为1月下旬至3月上旬;大麻/葎草属占第三位,花粉含量5112粒/1000 mm 2,占全年花粉总量的3.66%,其播粉期为8~10月,高峰期为8月下旬至10月下旬。上述3种花粉为本地区优势气传花粉,合计占全年花粉总量的81.98%。含量超过1%的花粉还依次包括银杏、桦木属、松属、悬铃木属、枫杨属及蒿属花粉。全年花粉含量月分布呈现2个高峰,第一高峰为1~5月,主要气传花粉为构属、柏科、银杏、桦木属、松属、悬铃木属、枫杨属等树木花粉,占全年花粉总量的89.38%。第二高峰为8~10月,主要气传花粉为大麻/葎草属、蒿属等莠草花粉,占全年花粉总量的5.04%。结论本地区花粉季节有两个:春季为主要花粉季节,主要气传花粉为构属、柏科等树木花粉;夏秋季为次要花粉季节,主要花粉气传为大麻/葎草属、蒿属等莠草花粉。构属、柏科、大麻/葎草属花粉为本地区优势气传花粉,构属花粉为本地区绝对优势气传花粉。     

Analysis of allergenic components of Bermuda grass pollen by monoclonal antibodies

A panel of 16 monoclonal antibodies (MoAbs) directed against Bermuda grass (Cynodon dactylon) pollen (BGP) were generated for identification and purification of the major allergenic components of the eliciting antigen (Ag). Radioimmunoprecipitation (RIP) analysis revealed that there were at least eight antigenic components with molecular weights (MW) ranging from 12 kilodalton (12 kDa) to 200 kDa. Each of these components has distinct biochemical characteristics based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). Among them, Cyn d Bd67K and Cyn d Bd58K were basic proteins, Cyn d Bd35K consisted of at least four isomeric components with isoelectric points ranging from 6.2 to 7.2. The other antigens (Cyn d Bd68K, 48K, 38K, Cyn d Bd200K, Cyn d Bd46K, Cyn d Bd25K and Cyn d Bd12K) were all acidic proteins. The IgE binding capacity of all these antigens was determined with sera from 11 BGP-allergics by using a modified radioallergosorbent test. All but one of the antigens (Cyn d Bd200K) were found to react with human IgE from sera of BGP-allergic patients. Among those human IgE-binding molecules, Cyn d Bd35K reacted with allergic sera most frequently (10 of 11), followed by Cyn d Bd58K (8 of 11) and Cyn d Bd46K (7 of 11) respectively. Our results suggest that Cyn d Bd35K, Cyn d Bd58K, and Cyn d Bd46K are major allergens of BGP, and the MoAbs we obtained should be valuable tools for further purification of these allergens.     

鲁南地区大气花粉及其致病性探讨

目的 探讨鲁南地区气传花粉的种类，分布，数量及其致病性。方法 采用空气曝片的方法，连续2年收集气传花粉，在光学显微镜下进行阅片鉴定，并将采集到的花粉处理后，制备成花粉变应原疫苗，进行皮肤试验。结果 2年共收集花粉10813粒，18个科属，每年3－5月和8－9月出现飘散高峰。主要致敏花粉是蒿属，Lu草，杨树及松树花粉。结论 鲁南地区的气传花粉主要包括Lu草属，松属，杨属，蒿属，藜属，柳属，榆属，禾本科，地肤，臭椿属，蓖麻属，柏科，核桃，向白葵属，栎属，桦属，银杏属，豚草等，以Lu草，松属，杨属，蒿属，藜属，禾本科花粉量较大，占70％。主要致敏花粉是蒿属，Lu草，杨树及松树花粉。