感染和炎症反应与冠心病的相关性研究

目的：探讨肺炎衣原体(Cp)、幽门螺杆菌(Hp)和巨细胞病毒(CMV)感染与全身炎症反应和冠心病的关系。方法：将疑有冠心病行冠脉造影检查者根据造影结果分为冠心病组(45例)和对照组(33例)。测定血清学Cp、Hp和CMV抗体及C反应蛋白(CRP)水平。结果：(1)Cp IgG、Hp IgG和IgA阳性率均与冠心病相关(P＝0．017、P＜0．001、P＝0．009)。去除冠心病传统危险因子影响后，仍保持相关性；(2)冠心病组血清CRP较对照组明显升高(P＜0．001)，经多因素分析后仍有统计学意义(P＝0．03)；(3)Cp IgG、HpIgG和CMV IgG阳性率与高基础CRP水平无关。三种病原体IgG阳性组和两种或两种以下病原体IgG阳性组高CRP比率分别为32．1％和14．9％，但经统计学分析无意义(P＝0．078)。结论：Cp和Hp慢性感染与冠心病相关，而CMV感染与冠心病无关。尚缺乏证据证明冠心病患者全身炎症反应的增强与Cp和Hp等感染之间的相关性。     

240例不孕症患者解脲支原体和衣原体检测结果分析

目的:了解不孕症患者解脲支原体(UU)和衣原体(CT)的感染状况及其与不孕症的关系。方法:对240例行输卵管造影的不孕患者(观察组)和152例正常生育的孕妇(对照组)进行宫颈分泌物的UU和CT检测。结果:UU感染率观察组为65.8%,对照组为44.1%,两组比较差异有统计学意义(P<0.001)。其中有输卵管炎症的不孕症患者中解脲支原体感染明显高于输卵管正常的不孕症患者,差异有统计学意义(P<0.001)。观察组中仅2例存在衣原体感染。结论:UU与不孕症密切相关,对不孕症患者行UU常规检测和及时治疗是必要的,CT检测可不作为常规检查项目。     

妊娠期妇女生殖道感染的分析

目的 了解无锡市妊娠期妇女的生殖道感染情况,为保障母婴健康提供依据.方法 采集1117例妊娠期妇女阴道或宫颈分泌物进行生殖道感染检测.结果 无锡市1117例妊娠期妇女生殖道感染患病率为37.5%;支原体性阴道炎患病率为16.2％,衣原体性阴道炎患病率为0.8％,滴虫性阴道炎患病率为1.8％,细菌性阴道病(BV)患病率为3.7％,淋病患病率为0.4％,梅毒患病率为1.3％,外阴阴道假丝酵母菌感染患病率为13.3％;在不同户籍、职业、文化程度的孕妇中衣原体性阴道炎、滴虫性阴道炎、梅毒检出率差异有统计学意义(P＜0.05).结论 无锡地区妊娠期妇女的生殖道感染率较高,应重视妊娠期妇女的生殖道感染常规筛查工作.     

203例输卵管积液不孕患者病原体分析

目的 了解输卵管积液不孕的病原体感染状况,寻找该病的主要致病菌,为该疾病的临床合理治疗提供研究依据.方法 对203例输卵管积液不孕患者采集宫颈分泌物和输卵管积液标本,对标本进行病原体培养鉴定与药敏试验检测,将宫颈分泌物和输卵管积液的病原体培养与药敏试验结果进行比较分析.结果 203例输卵管积液不孕患者的宫颈分泌物和输卵管积液中培养出病原体628株,其中支原体属、衣原体属331株占52.71％,以沙眼衣原体为主,占27.39％,革兰阳性菌155株占24.68％,以金黄色葡萄球菌为主,占11.31％,革兰阴性菌125株占19.90％,以大肠埃希菌为主,占10.83％,真菌17株占2.71％,以白色假丝酵母菌为主,占2.39％;203例输卵管积液不孕患者的宫颈分泌物中培养出的沙眼衣原体、解脲脲支原体、二者混合感染的阳性率分别为43.84％、39.41％、13.79％,输卵管积液分别为40.89％、38.92％、12.81％,二者比较差异均无统计学意义.结论 临床治疗输卵管积液不孕时,应根据病原体检测结果合理使用抗菌药物;在制定治疗方案时应着重考虑沙眼衣原体、解脲脲支原体感染的治疗,还要重视对输卵管、宫颈及其他受感染部位的协同治疗.     

脑梗死患者肺炎衣原体感染与血清基质金属蛋白酶-9的关系分析

目的 通过观察脑梗死患者肺炎衣原体(CP)感染血清基质金属蛋白酶-9 (Matrix metallopeptidaseq,MMP-9)含量变化,以探讨CP感染与脑梗死的关系.方法 选择2012年1-7月脑梗死住院患者为脑梗死组,选择同期健康体检者为对照组,每组各76例;应用多聚合酶链反应(PCR)技术检测两组,同时用酶联免疫吸附测定法检测MMP-9.结果 两组在临床资料、基础疾病和吸烟等比较差异无统计学意义;脑梗死组阳性43例,阳性率为56.58％,对照组CP-DNA阳性16例,阳性率为21.05％,两组比较差异有统计学意义(x2=152.63,P＜0.05);脑梗死组MMP-9质量浓度为(651±89)ng/ml,对照组为(555±91)ng/ml,差异有统计学意义(t=5.83,P＜0.01);血清CP-DNA阳性脑梗死患者的MMP-9质量浓度为(470±98)ng/ml,高于CP-DNA阴性脑梗死患者的(305±60)ng/ml,差异有统计学意义(t=11.28,P＜0.01).结论 CP慢性感染与脑梗死发病有关,CP可能通过炎症反应在脑梗死的发生、进展中发挥重要作用.     

Transmission of antibodies to Chlamydia Psittaci and Coxiella Burnetii through eggs and “crop milk” in pigeons

Young semi-domesticated pigeons captured or hatched from eggs gathered in Bratislava during 1989–1991 were examined for complement fixing antibodies to Chlamydia psittaci and agglutinating antibodies to Coxiella burnetii. Antibodies to Ch. psittaci were present in 76% of birds younger than 24 h, in 47.7% between 1 and 10 days of age and in 12% of nestlings over 10 days old. Antibodies to Ch. psittaci were also detected in crop milk of 4.1% of 1 to 10 day old birds and in 4.5% of specimens older than 10 days. Antibodies to C. burnetii were not found in juvenile birds under 24 h old, but antibodies against this agent were present in 16.4% birds between 1 and 10 days old and in 18% over 10 days old. Antibodies to C. burnetii were also detected in crop milk collected from crops of 2% of the young birds between 1 and 10 days.     

Evaluation of a broadly protective Chlamydia-cholera combination vaccine candidate

The need to simultaneously target infections with epidemiological overlap in the population with a single vaccine provides the basis for developing combination vaccines. Vibrio cholerae ghosts (rVCG) offer an attractive approach for developing vaccines against a number of human and animal pathogens. In this study, we constructed a multisubunit vaccine candidate co-expressing the serovar D-derived Porin B and polymorphic membrane protein-D proteins of Chlamydia trachomatis and evaluated its ability to simultaneously induce broad-based chlamydial immunity and elicit a vibriocidal antibody response to the Vibrio carrier envelope. Intramuscular (IM) immunization with the vaccine candidate elicited high levels of antigen-specific genital mucosal and systemic Th1 cell-mediated and humoral immune responses against heterologous serovars and strains, including serovars E-H and L. Also, in addition to the multisubunit vaccine, the single subunit constructs conferred significant cross protection against the heterologous mouse strain, Chlamydia muridarum. Furthermore, all mice immunized with rVCG vaccine constructs responded with a significant rise in vibriocidal antibody titer, the surrogate marker for protection in cholera. These findings demonstrate the ability of the multisubunit vaccine to induce cross protective chlamydial as well as vibriocidal immunity and establish the possibility of developing a broadly efficacious Chlamydia-cholera combination vaccine.     

Amphipols stabilize the Chlamydia major outer membrane protein and enhance its protective ability as a vaccine

The native major outer membrane protein (nMOMP) from Chlamydia was purified in its trimeric form using the zwitterionic detergent Z3-14. In aliquots from this preparation, Z3-14 was exchanged for amphipol (APol) A8-35. CD analysis showed that trapping with A8-35 improved the thermostability of nMOMP without affecting its secondary structure. Recombinant MOMP (rMOMP) was also formulated with Z3-14 or A8-35. Four groups of mice were vaccinated with nMOMP/Z3-14, nMOMP/A8-35, rMOMP/Z3-14 or rMOMP/A8-35 using CpG and Montanide as adjuvants. A positive control group was inoculated intranasally with live Chlamydia and a negative control group with culture medium. Mice were challenged intranasally with live Chlamydia and protection was assessed based on changes in body weight, the weight of the lungs and the number of chlamydial inclusion forming units recovered from the lungs 10 days after the challenge. Overall, vaccines formulated with nMOMP elicited better protection than those using rMOMP. Furthermore, the protection afforded by nMOMP/A8-35 was more robust than that achieved with nMOMP/Z3-14. In contrast, no differences in protection were observed between rMOMP/Z3-14 and rMOMP/A8-35 preparations. These findings suggest that the higher protection conferred by nMOMP/A8-35 complexes most likely results from a better preservation of the native structure of MOMP and/or from a more efficient presentation of the antigen to the immune system, rather than from an adjuvant effect of the amphipol. Thus, amphipols can be used in vaccine formulations to stabilize a membrane-protein component and enhance its immunogenicity.     

鹦鹉热衣原体肺炎2例并文献复习

鹦鹉热衣原体为不典型致病菌的一种,为胞内寄生的革兰阴性菌,鸟类为最主要宿主,为人畜共患病。本文报道上海市松江区中心医院收治的2例鹦鹉热衣原体感染肺炎,其中1例合并呼吸衰竭、肝功能不全,经支气管镜肺泡灌洗液行二代基因测序后明确病原菌感染,后经抗感染后肺部炎症吸收好转。通过病例分享,为临床医师在诊治肺炎的临床过程中提供参考...     

地红霉素治疗男性非淋菌性尿道炎疗效观察

目的:比较地红霉素和红霉素治疗非淋茵性尿道炎的疗效和安全性.方法:选择男性支原体和衣原体无合并症尿道炎患者129例为研究对象,试验组67例给予地红霉素250 mg,po qd,对照组62例给予红霉素片500mg,po qid,均连续服用14 d.结果:地红霉素组的治愈率为76.1%,有效率为89.6%,病原茵清除率为76.1%;红霉素组治愈率为48.4%,有效率为66.1%,病原茵清除率为48.4%.结论:地红霉素治疗非淋茵性尿道炎安全有效.