血液灌流抢救重度急性有机磷中毒的疗效观察和护理体会

目的 探讨血液灌流（HP）抢救重度急性有机磷中毒（AOPP）的临床疗效及护理。方法 将确诊为重度AOPP的106例患者随机分为治疗组和对照组,均给予清除毒物、解毒及对症支持等常规内科治疗,治疗组同时给予HP治疗,观察两组患者的临床疗效及不良反应。结果 治疗组总治愈率92.59%,与对照组比较差异具有统计学意义（71.15%,P〈0.01）,且未出现明显不良反应。结论 HP抢救重度AOPP患者,疗效满意,安全性好,值得临床推广。     

CagA的表达对慢性胃炎Th细胞亚群分化的影响*

目的探讨幽门螺杆菌CagA的表达对慢性胃炎患者胃黏膜Th细胞亚群分化的影响。方法80例慢性胃炎患者，ELISA法测定血清CagA抗体，根据结果将慢性胃炎患者分为CagA阳性和阴性组，均取患者胃黏膜行病理检查及PCR、Western blot检测核转录因子TBX21、GATA-3、FoxP3和Rorγt的表达水平。结果 CagA抗体阳性组和阴性组分别为52例和28例，CagA抗体阳性组炎症中、重度43例（82.7%）显著高于阴性组10例（35.7%），差异有统计学意义（χ2=17.964，P<0.05）；2组间Hp菌密度差异无统计学意义。CagA抗体阳性组TBX21、Rorγt的转录及蛋白表达水平较阴性组显著下调，而GATA-3、FoxP3的转录及蛋白表达水平较阴性组显著上调。结论慢性胃炎CagA阳性患者的胃黏膜炎症程度更重，但机体却不能有效清除Hp在胃黏膜中的定植，其原因与可能存在Th0向Th2/Treg的分化偏倚有关。     

幽门螺杆菌与口腔扁平苔藓关系的探讨

目的探讨HP与OLP之间的关系方法对50例口腔扁平苔藓OLP分组作幽门螺杆菌检测一组采用快速尿素酶试验12例HP检出率83.33%二组14C一尿素呼吸试验21例HP检出率61.90%三组HP抗体金标快速检测17例HP检出率76.47%结果50例OLP中HP阳性为36例其中伴有粘膜充血、糜烂或牙龈上皮剥脱、红亮者22例HP检出率为100%结论我们认为HP与OLP之间存在着密切关系     

Monoliths with chiral surface functionalization for enantioselective capillary electrochromatography

The state-of-the-art in CEC enantiomer separations with monolithic capillary columns is comprehensively reviewed. The various types of monolithic columns comprising in situ organic polymer monoliths, molecularly imprinted polymer (MIP) monoliths, silica monoliths and monoliths made from particles are discussed with a focus on materials’ synthesis, chemistry and properties as well as column aspects. Monolithic MIP-type porous layer open-tubular (PLOT) columns are treated herein as well. From this survey of the literature, the authors come to the conclusion that monolithic silica capillaries appear to become the preferred column type for CEC enantiomer separations of low-molecular drugs and other chiral pharmaceuticals or chemicals.     

幽门螺杆菌与口腔溃疡型疾病的相关性研究

探讨HP与口腔溃疡型疾病的相关性。方法 :采用 1∶1配对的方法 ,对 12 7例临床症状表现为口腔溃疡的患者与正常对照组分别进行PCR检测 ,治疗时分铋制剂组和常规治疗组进行对比。结果 :三种分型的患者HP感染率与对照组均呈显著性差异 ,采用铋制剂和灭滴灵的治疗效果与常规治疗亦具有显著性差异。结论 :HP参与了口腔内多种溃疡型疾病的形成 ,抑或是参与、加强了其它因素的致病过程。     

半夏泻心汤加味治疗消化性溃疡53例疗效观察

[目的]观察半夏泻心汤加味治疗消化性溃疡的疗效。[方法]将85例患者随机分为治疗组（53例）和对照组（32例）,治疗组服用半夏泻心汤加味,加服雷贝拉唑片;对照组服用雷贝拉唑片;两组中如有HP阳性者,第一周服用杀HP三联药物。[结果]治疗组总有效率为96.23%,对照组81.25%;半年后复发率治疗组（9.68%）,对照组（33.33%）,治疗组疗效明显优于对照组（P〈0.05）。[结论]半夏泻心汤加味治疗消化性溃疡有良好疗效。     

胃炎及消化性溃疡患者血清Hp CagA IgG的检测

目的 cagA 基因阳性 Hp 感染与慢性胃炎、消化性溃疡的关系。方法用 ELISA 检测55例慢性胃炎和33例消化性溃疡患者血清中 Hp CagA IgG 水平。结果慢性胃炎患者的 CagA IgG 阳性率为47.3％,其阳性血清的平均 CagA IgG 水平为27.9±12.9U/ml,而消化性溃疡患者之阳性率为72.7％,CagA IgG 水平为41.6±18.0U/ml,两组比较相差显著(p<0.05和 p<0.01)。结论 CagA 阳性 Hp 具有致溃疡作用。     

Differential oncogenic potential of geographically distinct Helicobacter pylori CagA isoforms in mice

Infection with cagA‐positive Helicobacter pylori is associated with gastric carcinoma. The cagA‐encoded CagA protein is delivered into gastric epithelial cells and, upon tyrosine phosphorylation at the C‐terminal EPIYA segments, binds and deregulates SHP‐2 oncoprotein. On the basis of the differential alignment of the EPIYA segments, CagA can be subdivided into Western CagA, which is produced by H. pylori isolated in Western countries, and East Asian CagA, which is produced by H. pylori circulating in East Asian countries. Western CagA contains EPIYA‐A, EPIYA‐B and variable numbers of EPIYA‐C segments, whereas East Asian CagA contains EPIYA‐A, EPIYA‐B and variable numbers of EPIYA‐D segments. Upon tyrosine phosphorylation, EPIYA‐C and EPIYA‐D, respectively, serve as low‐affinity and high‐affinity SHP‐2‐binding sites. We previously reported that systemic expression of East Asian CagA (CagA‐ABDD) induces gastrointestinal and hematopoietic malignancies in mice. In this study, we generated transgenic mice that systemically express Western CagA (CagA‐ABCCC), the levels of which are comparable to those in mice expressing East Asian CagA. The mice developed gastric epithelial hypertrophy and gastrointestinal tumors and also showed lymphoid abnormality but not myeloid abnormalities such as granulocytosis and myeloid leukemia found in mice carrying East Asian CagA. The incidence of tumors in mice expressing Western CagA was significantly lower than that in mice expressing East Asian CagA. Our results indicate that Western CagA is qualitatively less oncogenic than East Asian CagA. Differential oncogenic potential of geographically distinct CagA isoforms may contribute to the differential prevalence of gastric carcinoma between East Asian countries and Western countries. © 2009 UICC     

抑酸与不同抗菌药治疗幽门螺杆菌相关性消化性溃疡的对照研究

目的:评价洛赛克、羟氨苄青霉素二联疗法对幽门螺杆菌(HP)相关性消化性溃疡的疗效。方法:将83例HP阳性的胃和十二脂肠溃疡患者按就诊顺序分为A、B两组。A组43例,每次口服洛赛克20mg,1天1次,21天,羟氨苄青霉素500mg,1天3次,14天。B组40例,在A组服药的基础上再给甲硝唑400mg,1天3次,14天。结果:两组溃疡愈合率分别为93.0%、95.0%,HP消除率分别为81.4%、85.0%。结论:两组溃疡治愈率和HP消除率差异无显著性(P>0.05),B组副作用明显高于A组(P<0.05)。     

血液透析串联血液灌流与血液透析滤过疗效对比

目的:观察血液灌流+血液透析(HP+HD)与血液透析滤过(HDF)对维持性血透(MHD)患者对大分子物质清除作用以及对患者营养状态和生活质量的影响。方法:选取复旦大学附属金山医院肾内科血透室MHD患者60例,随机分为HDF组和(HP+HD)组,每组30例。检测瘦素(Leptin)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和C反应蛋白(CRP)等水平,计算上述各分子下降率,评估患者营养状况、生活质量,对比两组间差异。结果:(HP+HD)组Leptin、IL-6及TNF-α清除率高于HDF组。治疗6个月后,(HP+HD)组患者炎症标志物IL-6、TNF-α、CRP水平均显著低于HDF组,且营养状态及生活质量优于HDF组。结论:HP+HD可以有效降低大分子物质水平,减轻炎症状态,改善营养状态及生活质量。