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81.
P. KORKOLOPOULOU J. CORDELL M. JONES L. KAKLAMANIS A. TSENGA K.C. GATTER D.Y. MASON 《Histopathology》1994,24(6):511-515
Recent evidence indicates that membrane-bound immunoglobulin on B lymphocytes is associated with a molecule which comprises the products of the mb-1 and B29 genes. This molecule is a highly specific marker for B-cells, presumably because of its central functional role in antigen triggering, and has recently been clustered as CD79a at the 5th Leucocyte Workshop. Recently there has been controversy surrounding reports of B-cell antigen expression by Reed–Sternberg and related cells, and we have therefore studied 108 cases of Hodgkin's disease immunohistochemically using a novel antibody which detects mb-1 protein in paraffin sections. The results were compared with those achieved using antibody L26 to detect CD20. The mb-1 protein was present in the neoplastic cells in all 14 cases of lymphocyte predominance Hodgkin's disease studied, and CD20 immunoreactivity was also found in seven of the eight cases of this subtype studied. Of the non-lymphocyte predominance cases, 20% (19/94) expressed mb-1 and 30% (20/67) CD20 in the Reed–Sternberg cells, but the cells positive for either of these two markers usually constituted only a very small proportion of the neoplastic population. However, in occasional cases (one of 94 for mb-1 and five of 67 for CD20), more than 50% of the neoplastic cells expressed one or both B-cell antigens. These results confirm the B-cell origin of the neoplastic cells in lymphocyte predominance Hodgkin's disease, but they also indicate that, contrary to our previous study, mb-1 expression may occasionally be found in what appears, on histological grounds, to be other types of Hodgkin's disease. 相似文献
82.
83.
Corinna S. Bowser Jean Kaye Rauno O. Joks Cascy-Arnoux Charlot H. Jack Moallem 《Pediatric allergy and immunology》2007,18(4):298-303
Elevated serum immunoglobulin E (IgE) and increased prevalence of atopy is reported in patients infected with human immunodeficiency virus (HIV). The elevated serum IgE may be attributed to polyclonal stimulation of B cells or IgE production against allergens, viruses, fungi and bacteria. This study investigates the prevalence of atopy in perinatally HIV-infected children, and the relationships between serum IgE (and other serum immunoglobulins) with atopy, CD4+ cell count and HIV-disease stage. Serum immunoglobulin levels, epicutaneous skin test for common aeroallergens, clinical Centers for Disease Control and Prevention (CDC) classification, CD4+ cell counts and allergy history were extracted from the charts of perinatally HIV-infected children on highly active antiretroviral therapy. The prevalence of atopy (52%) and the pattern of aeroallergen sensitivity were comparable with the US pediatric population. Serum IgE levels did not correlate with clinical disease stage. However, in non-atopic patients, serum IgE levels increased with disease progression (p = 0.02). There was an inverse relationship between the prevalence of elevated serum IgE levels and atopy with progression of disease (p = 0.019). Serum IgE did not correlate with atopy, CD4+ cell count, or duration of HIV infection or levels of serum immunoglobulins. This is the first study to show no increased prevalence of atopy in perinatally HIV-infected children compared with the general population. In advanced stages of HIV, elevated serum IgE may be specific for antigens other than those known as allergens. 相似文献
84.
The popularization and application of cold storage red blood cells or whole blood at −80 °C of the Rh(D) negative patients in surgical operation 总被引:2,自引:0,他引:2
Summary The efficiency of cold storage red blood cells (CSRBC) or whole blood at −80 °C used in 27 Rh(D) negative patients during
surgical operation was reported. The Rh(D) negative patients received the transfusion of CSRBC or whole blood stored at −80
°C for 180 to 360 days. The changes in the indexes, such as blood TB, DB, K+, Na+, BUN, Cr, urine protein (URPO), UOB, Hb, HCT, serum total protein, relative to hemolytic reaction and blood volume before
and after transfusion were observed. The results showed that after transfusion of CSRBC or whole blood 27 cases were negative
for urine protein and UOB, and the levels of BUN and Cr were normal (P>0.05). Blood TB, DB, Hb, and HCT were increased, while pH, blood K+ and blood Na+ was normal with the difference being not significant before and after operation (P>0.05). Plasma protein was decreased, but there was no significant difference before and after operation (P>0.05). It was suggested that CSRBC or whole blood at −80 °C could be safely infused to the Rh(D) negative patients without
side effects during the surgical operation.
YU Zhongqing, male, born in 1957, Technician in Charge 相似文献
85.
M. STEWARD R. BISHOP N.H. PIGGOTT I.D. MILTON B. ANGUS C.H.W. HORNE 《Histopathology》1997,30(1):16-22
Phenotypic analysis of lymphoproliferative disorders is now considered mandatory for accurate classification which is the basis for optimum patient management. This is presently carried out in most cases using a range of antibodies recognizing B and T-cell antigens effective in paraffin sections, and an antibody to CD3 is currently a key member of such panels, indicating T-cell phenotype. Current antibodies to CD3 are polyclonal with the inherent disadvantages of this type of reagent compared to monoclonal antibodies. In this study, we have used a recombinant fusion protein representing part of the epsilon subunit of the CD3 molecule to generate a novel monoclonal antibody (NCL-CD3-PS1) effective in paraffin sections. The antibody has been characterized biochemically and by immunohistochemistry using a wide range of normal and pathological tissues. Lineage and phenotype specificity have been supported in our study and results from other laboratories are awaited with interest. 相似文献
86.
87.
目的:探讨CD8^+CD28^+T细胞钙离子浓度的变化与心脏移植急性排斥反应(AR)的相关关系。方法:用流式细胞仪检测正常SD大鼠组(T0组)、同种心脏移植组(T1)(Wistar→SD大鼠)术后第1、3、5、7天CD8^+CD28^+T细胞内钙离子浓度的变化,分析CD8^+CD28^+T细胞内钙离子浓度的变化与心脏移植AR程度的相关关系。结果:CD8^+CD28^+T细胞内钙离子浓度升高与AR严重程度成正相关,相关系数为0.469,相关具有显著性(P〈0.05)。结论:心脏移植后CD8^+CD28^+T细胞内钙离子浓度显著升高,其升高程度与AR严重程度成正相关。 相似文献
88.
给出了用“混合型”雅可比级数的泰勒平均逼近黎普希兹函数类的点态估计。 相似文献
89.
在标记脐血造血祖细胞表面抗原(HPCA),CD34中,比较抗-HPCA-2-FITC和Tk3(纯抗体)标记的CD(34+)细胞在流式细胞仪分析中的荧光特征及两种单抗标记的CD(34+)细胞与体外培养的粒单细胞集落形成单位(CFU-GM),红系爆发形成单位(BFU-E),和混合集落形成单位(CFU-Mix)的相关性。结果发现脐血有核细胞中,抗HPCA-2阳性细胞占1.05±0.72%(n=13),Tk3阳性细胞占2.06±1.25%(n=8),差别显著(P<0.05)。每毫升脐血两种抗体标记的细胞分别为96.56±56.64和231.40±163.93(P<0.05)。尽管HPCA-2阳性细胞与Tk3阳性细胞数量呈显著正相关(r=0.875,P<0.01),前者与CFU-GM,BFUE,CFU-Mix及集落总数CFUs均呈正相关,而后者仅与CFU-GM,CFUs相关。研究提示在检测造血祖细胞时,用抗-HPCA-2-FITC代替Tk3可降低假阳性,获得较好的OD(34+)细胞与CFU间的线性关系。 相似文献
90.
Immunosuppression of immunoglobulin synthesis seen in patients with multiple myeloma is in part due to immunosuppressive CD5 positive B cells. In a 13 year longitudinal study of an IgA-deficient blood donor who developed multiple myeloma, the presence of immunosuppressive CD5 positive B cells and T cells preceded the diagnosis of overt multiple myeloma and the appearance of immunosuppressive monocytes. These data argue that certain immune defects may be involved in the development of myeloma and are not simply a consequence of overt malignancy. 相似文献