^60Co消毒与高温高压消毒胚胎骨的实验研究

在12只健康家兔下颌骨体部的两侧各造成0.7cm×0.5cm×0.3cm的骨缺损区，分别置入^60Co消毒的胚胎骨和高温高压消毒的胚胎骨，发现高温高压消毒可以替代^60Co消毒胚胎骨进行移植，为临床提供了一种新的用作同种异体移植胚胎骨的消毒方法。     

穿琥宁联合病毒唑治疗小儿喘息性肺炎

目的 :观察穿琥宁联合病毒唑治疗小儿喘息性肺炎的疗效。方法 :65例喘息性肺炎患者随机分治疗组和对照组 ,治疗组 3 0例用穿琥宁、病毒唑静脉点滴 7天 ,对照组 3 5例用病毒唑、青霉素静脉点滴 7天。结果 :穿琥宁组的临床治愈率为 83 .3 % ,总有效率 96.7%。对照组临床治愈率为 5 7.1% ,总有效率 71.4%。经统计分析 ,两组疗效有显著性差异 ,χ2 =7.90 ,P <0 .0 1。结论 :穿琥宁联合病毒唑治疗喘息性肺炎治愈率高 ,疗效满意。     

活体胃血供的特点及其临床意义

目的 了解活体胃血供的特点，旨在探索中晚期胃癌的局部灌注化疗方法。方法 对36例胃溃疡患者在行胃大部切除治疗时，进行亚甲蓝染色，观察活体胃各分支动脉自然血供区域和血流阻断后血供范围的变化。结果 术中见左、右胃网膜动脉和左、右胃动脉分别为胃大、小弯近、远段相应区域提供血液，胃壁组织染色时间很短；阻断动脉血流后，染色范围扩展，染色时间延长；阻断伴行静脉后，染色范围进一步扩展，染色时间再度延长。结论 胃左动脉和胃网膜右动脉为胃血供的主要动脉，其他动脉分支为辅助性动脉；一般情况下，经动脉灌注的亚甲蓝在胃组织停留时间极短；阻断伴行静脉后，可使该染料在组织停留时间延长。推测此法可使化疗药物在胃组织中存留时间延长，从而有利于胃癌的局部化疗。     

High serum levels of tumour necrosis factor‐α and interleukin‐8 in severe asthma: markers of systemic inflammation?

BACKGROUND: Severe asthma is characterized by elevated levels of pro-inflammatory cytokines and neutrophilic inflammation in the airways. Blood cytokines, markers of 'systemic' inflammation, may be a feature of amplified inflammation in severe asthma. OBJECTIVE: To detect differences in IL-8, TNF-alpha, IL-16 and IL-13 levels in the serum(s) of stable severe and mild-moderate asthmatics related to blood leucocytes proportion, airway calibre and exhaled nitric oxide (NO) levels. METHODS: We assessed cytokine serum levels by ELISA and blood leucocyte counts by an alkaline peroxidase method in 20 healthy controls, 22 mild-moderate [forced expiratory volume in 1 s (FEV1)(%pred): 89+/-3] and 14 severe asthmatics [FEV1(%pred): 49+/-2]. RESULTS: IL-8 and TNF-alpha levels were higher in severe asthmatics than in mild-moderate asthmatics or in controls (P<0.05). No differences in IL-16 and IL-13 levels were detected. Severe asthmatics showed higher circulating neutrophil and eosinophil number than controls (P<0.05). In severe asthmatics, exhaled NO levels were superior than in controls (P<0.05), but inferior than in mild-moderate asthmatics (P<0.05). We found positive correlation between TNF-alpha levels and exhaled NO (r=0.67; P=0.01) or circulating neutrophil counts (r=0.57; P=0.03) in severe asthmatics. CONCLUSION: sTNF-alpha and sIL-8 are markers of 'systemic' inflammation in severe asthmatics, in conjunction with augmented circulating neutrophils, suggesting the involvement of neutrophil-derived cytokine pattern in severe asthma.     

Cloning and expression of SLC1OA4,a putative organic anion transport protein

AIM:To determine if novel bile acid transporters may be expressed in human tissues.METHODS:SLC10A1 (NTCP) was used as a probe to search the NCBI database for homology to previously uncharacterized ESTs. The homology search identified an EST (termed SLC10A4) that shares sequence identity with SLC10A1 and SLC10A2 (ASBT). We performed Northern blot analysis and RT-PCR to determine the tissue distribution of SLC10A4. SLC10A4 was cloned in frame with an epitope tag and overexpressed in CHO cells to determine cellular localization and functional analysis of bile acid uptake.RESULTS:Northern analysis revealed that SLC 10A4 mRNA is ubiquitously expressed fn human tissues with the highest levels of mRNA expression in brain,placenta, and liver. In SLC10A4-transfected CHO cells,immunoblotting analysis and immunofluorescence staining demonstrated a 49-kDa protein that is expressed at the plasma membrane and intracellular compartments.Functional analysis of SLC10A4 showed no significant taurocholate uptake in the presence of sodium when compared to untransfected CHO cells.CONCLUSION:To date, we have shown that this protein has no capacity to transport taurocholate relative to SLC1041; however, given its ubiquitous tissue distribution, it may play a more active role in transporting other endogenous organic anions.     

Gln与rhGh对肝硬化病人肠黏膜屏障的影响

目的观察Gln与rhGh各单独应用及联合应用对肝硬化病人小肠黏膜屏障形态学和功能学的影响。方法20个肝硬化病人随机分成四组,给予等氮等热量营养支持7d,Gln+rhGh组口服Gln[0.3g/(Kg·d)]和皮下注射rhGh(10U/d);Gln组口服Gln;rhGh组应用皮下注射rhGh;CONT对照组应用相应安慰剂。观察治疗前后患者小肠黏膜通透性和十二指肠黏膜绒毛高度和隐窝深度的变化,分别比较。结果Gln+rhGh组治疗后小肠黏膜通透性有显著性的降低(P<0.05),且显著低于其他三组(P<0.05);十二指肠黏膜绒毛高度和隐窝深度有显著性的增高(P<0.05),且显著高于其他三组(P<0.05)。结论Gln与rhGh联合应用能减低肠道黏膜的通透性和维持其形态学的完整。     

NF-κB在肝细胞癌组织中的表达及意义

目的：探讨NF-κB基因在肝细胞癌组织中的表达及生物学意义。方法：采用逆转录聚合酶链反应(RT-PCR)、Western-blot免疫组织化学技术检测32例肝癌组织及癌旁肝组织中的NF-κB基因的mRNA和蛋白表达水平及蛋白定位。结果：NF-κB基因在肝癌组织中的表达较癌旁肝组织显著性增高(P<0.05)。免疫组化结果显示，NF-κB蛋白在肝癌细胞胞浆和胞核中均有表达，而在癌旁肝细胞中仅在胞浆中有表达。结论：NF-κB基因在肝癌组织中呈显著高表达，提示其可能参与了肝癌的发生发展。NF-κB表达定位在癌细胞和癌旁肝细胞中的差异，提示NF-κB活化后，进入胞核，通过调节下游基因的转录，促进肝癌的发生。     

口服抗原对巨噬细胞共刺激分子CD80/CD86表达的影响

目的探讨口服抗原对巨噬细胞(M)共刺激分子CD80/CD86表达的影响及其在诱导妊娠免疫耐受中的作用。方法自然流产小鼠(CBA/J×DBA/2)分为免疫组和未免疫组,免疫组分别口服滋养细胞膜抗原(TMA2)和卵清蛋白(OVA),以正常妊娠小鼠(CBA/J×BALB/c)作为对照组。采用双标记流式细胞分析技术,分别检测各组小鼠脾脏及肠系膜淋巴结(MLN)CD80 M和CD86 M的表达。结果MLN内,未免疫组CD80 M表达明显高于对照组(P<0.05),而CD86 M含量明显低于对照组(P<0.001);TMA2免疫组CD80 M含量明显低于未免疫组(P<0.05);OVA免疫组CD86 M含量明显高于未免疫组(P<0.001)。脾脏内,CD80 M表达在各组间比较无显著差异;CD86M表达,未免疫组明显低于对照组(P<0.05),TMA2免疫组显著低于未免疫组(P<0.05),OVA免疫组则显著高于未免疫组(P<0.001)。结论流产的发生与M表面共刺激信号CD80/CD86异常有关;口服适当抗原可改变CD80/CD86 M表达模式,诱导妊娠免疫耐受的形成。     

原发性胆囊癌CD44v5、CD44v6表达及其临床病理意义的研究

目的 检测胆囊癌组织中CD44v5、CD44v6的表达,并探讨其在胆囊癌发生发展过程中的临床病理意义.方法 采用SP免疫组织化学方法,检测49例胆囊癌、10例胆囊结石伴慢性胆囊炎组织和10例正常胆囊组织中CD44v5、CD44v6蛋白表达情况.结果 CD44v5、CD44v6阳性表达率在胆囊癌组织明显高于良性组织.在胆囊癌中,CD44v5基因表达阳性率与组织学分级无关,CD4 4v6基因表达阳性率与组织学分级有关.有淋巴结转移的胆囊癌组织中,CD44v5、CD44v6阳性表达率明显高于无淋巴结转移者.结论 CD44v5、CD44v6可能在胆囊癌的淋巴结转移中起着重要的作用,CD44v5、CD4 4v6可能是了解胆囊癌病变发生发展生物学行为,淋巴结转移的重要生物学指标.