The Study of Passive Membrane Properties and Morphology Reveals Neuronal Differences Along the Sagittal Axis of the Ventral Periaqueductal Grey Matter

The membrane properties of the neurons located in the ventral part of the periaqueductal grey (PAG) of the guinea-pig were studied using an in vitro slice preparation. Cells had low values of resting membrane potential (-53.3 +/- 1.3 mV, mean +/- standard error), high input resistance (195. +/- 16.2 M ohm) and moderate values of membrane time constant (12.6 +/- 0.7 ms). The last two parameters changed as recordings were made along the sagittal axis, higher values corresponding to the more rostral cells. Three main neuronal types-fusiform, triangular and stellate-were found in the ventral PAG using intracellular injection of Lucifer yellow. A study of the cell number and cell density was carried out in coronal and sagittal sections of the ventral PAG. This analysis showed a clear gradient of size in this region arising from the gradual disappearance of large (17 to 40 microm) neurons in the caudorostral direction. The neuronal density also increased in this direction. Therefore, some electrotonic and morphological parameters differ along the sagittal axis. These findings suggest a larger neuronal heterogeneity of the caudal part of the PAG, and might contribute to a functional segregation of this region.     

TTV核酸模板快速制备方法

目的 寻求建立适合大批量临床标本检测TT病毒（TTV）核酸快速制备方法,直接用于PCR扩增。方法 应用蛋白酶K裂解酚氯仿抽取法与直接快速裂解法,分别制备TTV核酸做PCR模板,用于TTV的聚合酶链反应的快速检测。结果 在30例非甲－非庚型肝炎血清和50例慢性乙型肝炎患血清,应用经典的蛋白酶K裂解酚氯仿抽取法和直接快速裂解法,分别制备TTV核酸模板,在相同条件下进行PCR扩增,结果两种制备方法的符合率为89％。结论 TTV是一种新发现的肝炎病毒,目前主要以实验室检测病毒核酸的存在为诊断依据,建立了具有快速、简便、适合大批量临床标本中TTV核酸制备方法,对TTV肝炎的快速诊断,具有重要的意义。     

复方辛夷滴鼻液的研制与临床应用

目的：对复方辛夷滴鼻液的工艺和质控标准进行了研究。方法：通过改进工艺,采用提取挥发油和煎煮相结合的方法。结果：该法工艺合理、质量可靠,所制滴鼻液对治疗鼻炎疗效高,有效率达８６．３％。结论：可用该法制备复方辛夷滴鼻液,并应用于临床。     

血液分离胶的制备研究

目的 :对血液分离胶的制备进行研究。方法 :用天然橡胶和特效触变剂等原料制备具有触变性的凝胶 ,研究该凝胶分离血清的较优组成。结果 :血液分离胶的较优组成是天然橡胶 12 .8%、特效触变剂 2 8.0 %和溶剂汽油 5 8.2 %。结论 :所制备的血液分离胶具有制备容易、分离效果好和成本较低等优点。     

升丹制剂对小鼠机械性创面微循环影响的实验研究

目的 通过不同浓度的升压制剂对创面微循环影响来控制其对创面愈合的作用机制。方法 应用彩色病理图文分析系统,分析创面微血管增生数、扩张度及微血栓数。结果 在造模后３天、７天,实验且数值均显著高于对照组。结论 升丹制课题可以和改善创面微循环,减少微血栓,增加创面营养和血供,有利创面愈合。     

三种烤瓷贴面牙体预备的临床修复效果评价

:【目的】评价 3种烤瓷贴面 (PLV)牙体预备的近期临床效果。【方法】根据贴面形态和覆盖牙体范围将PLV牙体预备分为I型、L型和U型 3种 ,用于着色牙和前牙间隙、缺损等修复 ,PLV采用长石质PLV瓷系统制作 ,双重固化树脂粘结系统粘结 ,观察 6～ 2 2个月 ,考察 3种牙体预备类型的适应证、最终美学效果、粘接强度及牙本质敏感性等。【结果】 35例 2 0 3个瓷贴面修复后形态、颜色满意 ,美学效果佳 ;无脱落 ,仅局部崩折 2例 ,近期粘接效果满意 ;备牙时牙本质暴露率达 95 16 % ,粘固后牙本质过敏症状不同程度缓解。【结论】合理选择适应证、足够的牙体制备及高强度树脂粘结系统 ,可保证 3种牙体预备类型均能使PLV获得良好的外形、满意的颜色及可靠的临床近期粘接强度 ;但牙本质过敏问题应予重视     

茯苓多糖颗粒剂的研制及质量检测

以提取的茯苓多糖为主要原料,制成颗粒剂保健品,并拟定质检项目其进行定性、定量检测。工艺简单易行,具有较好的治疗、保健功效。     

钙增敏剂MCI-154对内毒素休克大鼠左心室肌原纤维ATP酶活性的影响

目的　研究钙增敏剂MCI 15 4对内毒素休克大鼠左心室肌原纤维ATP酶活性的影响。方法　利用心室肌原纤维制备 ,测定其在不同钙浓度的激活液中的ATP酶活性。结果　内毒素休克组心肌肌原纤维在不同pCa(-log[Ca2 +] )溶液中的ATP酶活性及最大ATP酶活性均明显低于假休克组 ,ATP酶 pCa曲线向右移位约 0 .35个pCa单位 ,曲线的中位值pCa50 (产生 5 0 %最大ATP酶活性所对应的pCa)明显降低。 5×10 -5mol/L甲腈吡酮对上述异常无明显的纠正作用。内毒素休克大鼠心室肌原纤维经含 1× 10 -5mol/L的MCI 15 4的激活液处理后 ,各pCa点ATP酶活性及最大ATP酶活性均明显增加 ,显著高于内毒素休克组和甲腈吡酮组 ;ATP酶 pCa曲线向左移位约 0 .4个pCa单位 ,pCa50 值增加 ,显著高于内毒素休克组和甲腈吡酮组值 ,与假休克组值无明显差别。结论　内毒素休克后 ,心肌肌原纤维活力下降 ,ATP酶活性降低 ;钙增敏剂MCI 15 4可通过增加心肌收缩蛋白对钙的敏感性而提高心肌肌原纤维活力 ,增加ATP酶活性     

黄参方剂诱导胃癌癌前病变细胞凋亡和影响BCL—2蛋白表达的研究

目的：探讨黄参方剂对胃癌癌前病变细胞凋亡及相关基因表达的影响。方法：将７２例胃癌癌前变患者随机分为２组,治疗组３７例,口服黄参方剂６０ｍｌ／ｄ;对照组３５例,口服维酶素片１２片／天,疗程２月。治疗后胃镜同一部位取材,检测治疗前后细胞凋亡指数和ＢＣＬ－２蛋白的表达。采用原位末端标记法（ＴＵＮＥＬ）检测细胞凋亡,采用免疫组化检测ＢＣＬ－２蛋白的表达。结果：黄参方剂组服药前后细胞凋亡指数分别为（１９．８     

Ischaemia induced alternans of action potential duration in the intact-heart: dependence on coronary flow, preload and cycle length

Clinical and experimental evidence relate action potential duration(APD) alternans to ischaemic heart disease and ventricular arrhythmias.The present investigation was performed to study the quantitativerelationship between APD alternans and the degree ofischaemia,loading conditions and cycle length (CL) in an intact heart. Monophasic action potentials (MAP) were simultaneously recordedby contact electrodes from two left (LV) undone right ventricular(RV) sites in 20 Langendorff-perfused rabbit hearts. The preparationswere subjected to global ischaemia at flow rates ranging from40% of normal flow to complete cessation of flow. Pacing wasperformed at either constant or regularly changing CL. The magnitudeof APD alternans was expressed as beat-to-beat differences inaction potential duration of two consecutive MAPs. During normalper fusion, neither very fast pacing at a CL of 200 ms nor periodicalrate switches resulted in persistent APD alternans. Pacing ata constant CL of 800 ms did not induce A PD alternans at completecessation of flow for 6 min. However, alternans developed progressivelyat a constant CL of 400 ms after 2.8±0.3 min of completeischaemia at the pre-loaded LV, andafter 4.6±0.4 minat the unloaded RV (P<0.01). The reduction of preload atthe LV from 15 to 5 mmftg end-diastolic pressure delayed developmentof APD alternans from 2.8±0.3 min to 4.3±0.4 min(P < 0.05) at 400 ms CL. Following graded under per fusionof 40%, 20% and 10% of initial flow, persistent APD alternansdeveloped in relation to the degree of flow reduction and increasedprogressively with duration ofischaemia. APD alternans at theLV always preceded the onset of APD alternans at the RV. Inexperiments with identical flow rates the shortest CL of 200ms resulted in the greatest and earliest initiation of APD alternanscompared to the longer CL (P<0.01, P<0.001). An increasein CL from 400 to 800 ms immediately abolished APD alternans,generated by the shorter CL, at any time during the 6 min periodof complete ischaemia. Similarly, increasing the cycle lengthfrom 200 or 400 to 600 ms eliminated APD alternans up to 6 minof ischaemia and significantly reduced its magnitude between7 and 10 min within a few beats. We conclude that persistent APD alternans is a characteristicfinding in the rabbit heart during global ischaemia. It is asensitive parameter of the severity of ischaemia and dependson the degree and duration of ischaemia as well as on the preload.The CL appears to have an independent effect on the generationof APD alternans, which is functionally separate from the effectof CL on the ischaemic burden. An eventual impact of these observationscould be the application of APD alternans as a diagnostic toolin electrophysiological examinations of myocardial ischaemiain experimental and clinical settings.