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11.
Gérald Vanzetto Marc Janier Daniel Fagret Luc Cinotti Xavier André-Fouet Michel Comet Jacques Machecourt 《European journal of nuclear medicine and molecular imaging》1997,24(2):170-178
The best test presently available to ascertain residual viability within an infarct-related area involves the use of fluorine-18
fluorodeoxyglucose (FDG) to detect the persistence of some cellular metabolism. Rest reinjection of thallium-201 is a less
accurate alternative but is easy to perform. Iodinated fatty acids, which are used with standard gamma cameras, are proposed
as markers of cellular metabolism. This study was performed to assess the value of 16-iodo-3-methyl-hexadecanoic acid (MIHA)
as a marker of the residual cellular metabolism by comparison with FDG in patients with a recent myocardial infarction, and
to evaluate its contribution compared with the201Tl stress-redistribution-reinjection technique. Stress-redistribution-reinjection201T1 imaging, rest MIHA imaging and glucoseloaded FDG imaging were performed in 22 patients with recent myocardial infarction.
Out of the 628 myocardial segments obtained from the left ventricular analysis, 400 were hypoperfused (relative uptake <0.75
of maximum uptake on stress201T1 imaging), 177 of which were severely hypoperfused (relative uptake <0.50). Receiver operating characteristic (ROC) curves
for predicting metabolic myocardial viability with FDG were derived from the results in respect of (a)201T1 activity during exercise, redistribution and reinjection and (b) MIHA up-take, using the two FDG thresholds most commonly
considered to define metabolic viability (0.50 and 0.60). Analysis of the 400 hypoperfused segments demonstrated that201T1 reinjection was the most accurate test in predicting the presence of myocardial viability (area under the ROI curves=0.85
and 0.86 at the 0.50 and 0.60 FDG thresholds, respectively;P<0.05 vs other tests). The global predictive values of MIHA and201T1 reinjection were, respectively, 0.87 and 0.89 at the 0.50 FDG threshold (NS), and 0.82 and 0.87 at the 0.60 FDG threshold
(NS). When only the 177 severely hypoperfused segments were considered,201T1 reinjection remained the most accurate test (accuracy 0.84 at the 0.50 FDG threshold and 0.82 at the 0.60 FDG threshold),
while the accuracy of MIHA decreased significantly (0.78 at the 0.50 FDG threshold and 0.73 at the 0.60 FDG threshold,P<0.05 vs201T1 reinjection). In all circumstances, MIHA was less specific than201T1 reinjection for the detection of metabolic viability. In conclusion, in patients with recent myocardial infarction, MIHA
accurately detects the persistence of metabolic viability, but is not superior to201T1. 相似文献
12.
详细分析了参数记录数据在Tracerlab FX-FDG上的合成过程,以及造成合成故障的确切原因,从而得出正确的结论,即:对液体的添加过程是否正常,数据记录可提供准确信息,是解决问题可依赖的分析手段。 相似文献
13.
目的 探讨实质器官移植术后血浆中白介素18(IL-18)、白介素10(IL-10)、白介素4(IL-4)水平的检测在人巨细胞病毒(HCMV)感染中的意义。方法 对24例实质器官移植受者,用免疫组化法监测HCMV抗原血症。然后采用ELISA法测定血浆中IL-18、IL-10及IL-4的水平。结果 HCMV抗原阳性组IL-18的水平高于阴性组(P<0.01),IL-10及IL-4的水平低于阴性组(均P<0.05)。结论 血浆IL-18的水平可作为预测移植患者HCMV感染的一个有效指标,IL-10及IL-4的水平则可作为患者HCMV感染后体内免疫抑制程度的监测指标之一。 相似文献
14.
Markus Dietlein Klemens Scheidhauer Eberhard Voth Peter Theissen Harald Schicha 《European journal of nuclear medicine and molecular imaging》1997,24(11):1342-1348
Metastases of differentiated thyroid cancer may show different uptake patterns for fluorine-18 fluorodeoxyglucose and [131I]NaI. FDG positron emission tomography (PET), iodine-131 whole-body scintigraphy (131I WBS) and magnetic resonance imaging were performed in 58 unselected patients, and spiral computed tomography (CT) of the
lung in 25 patients. Thirty-eight patients presented with papillary carcinomas, 15 patients with follicular carcinomas and
five patients with variants of follicular carcinoma. Primary tumour stage (pT) was pT1 in 3, pT2 in 19, pT3 in 11 and pT4
in 25 cases. For the detection of metastases, FDG PET was found to have a sensitivity of 50%, 131I WBS a sensitivity of 61%, and the two methods combined a sensitivity of 86%. When FDG PET was limited to patients with elevated
thyroglobulin (Tg) levels and negative 131I WBS, the sensitivity of this algorithm was 82%. Of the 21 patients with lymph node metastases, seven presented with FDG
uptake but no iodine uptake. In four of them, a second FDG hot spot appeared in a lymph node metastasis of normal size. Five
of the seven patients underwent surgery. None of the eight patients with pulmonary metastases smaller than 1 cm exhibited
FDG uptake, while five of them had iodine uptake. All had positive results on spiral CT. In conclusion, FDG PET cannot be
substituted for 131I WBS. If the Tg level is elevated and 131I WBS is negative, FDG PET can be used to detect lymph node metastases and complements anatomical imaging. A spiral CT of
the lung is useful to exclude pulmonary metastases before planning a dissection of iodine-negative lymph node metastases.
Received 2 May and in revised form 8 July 1997 相似文献
15.
Terry Reed 《Clinical genetics》1991,39(5):391-395
Evidence from the literature is reviewed to suggest that when fingertip dermal ridge patterns in chromosomal deletion syndromes are characteristic of the opposite spectrum of the developmental scale from patterns found in cases trisomic for the same chromosomal region, the association may be a consequence of loci with growth regulatory functions. Evidence is presented that DNA markers at 18q21 should be the first candidate sequences to be used to test this hypothesis in families with fingertip arches segregating in an apparent autosomal dominant fashion. 相似文献
16.
外阴尖锐湿疣人乳头瘤病毒感染的检测与分型 总被引:2,自引:0,他引:2
张秀华 《菏泽医学专科学校学报》2004,16(3):14-15
目的 了解患外阴尖锐湿疣人乳头瘤病毒(HPV)6型和11型的感染情况及PCR方法对尖锐湿疣诊断与分型的临床意义。方法 采用PCR方法测定160例尖锐湿疣患病题组织或分泌物中HPV6/11型及HPVl6/18型的感染率。结果 HPV6/11型感染率为95%(152/160),HPVl6/18型感染率为5%(8/160),HPV6/11型 HPVl6/18型混合感染率为1.3%(2/160)。结论 外阴尖锐湿疣患以HPV6型和11型感染为主。PCR方法是一种比较适合临床HPV检测与分型的极为敏感和特异的诊断方法。 相似文献
17.
目的探讨血清IL10、IL18在肝炎肝硬化的发病机制中的作用。方法62例肝炎肝硬化患者根据childpugh分级法分为3组:childpughA级组19例、childpughB级组23例和childpughC级组20例。采用ELISA法检测3组患者和20例健康献血员血清IL10、IL18的水平。结果childpughA、B、C级组血清IL18水平均明显高于对照组(P均<0.01),childpughA级组血清IL10水平略高于对照组,但无显著性差异(P>0.05);childpughB、C级组明显低于对照组(P<0.05);有腹水组、无腹水组血清IL18水平均明显高于对照组(P均<0.01),有腹水组血清IL10水平明显低于无腹水组、对照组(P<0.05,P<0.01);血清IL10水平与血清IL8水平呈负相关(r=-0.51,P<0.01);血清白蛋白水平与血清IL10水平呈正相关(r=0.566,P<0.01),与血清IL8水平呈负相关(r=-0.315,P<0.01);血清凝血酶原活动度与血清IL10水平呈正相关(r=0.506,P<0.01),与血清IL18水平呈负相关(r=-0.463,P<0.01);血清总胆红素水平与血清IL8水平呈正相关(r=0.677,P<0.01),与血清IL10水平呈负相关(r=-0.339,P<0.01)。结论IL10、IL18在肝炎肝硬化的发病机制中起一定的作用,其水平与肝损害程度密切相关。 相似文献
18.
Bonnie J. Baty Lynn B. Jorde Brent L. Blackburn John C. Carey 《American journal of medical genetics. Part A》1994,49(2):189-194
Developmental data were abstracted from medical records on 50 trisomy 18 individuals ranging in age from 1 to 232 months and 12 trisomy 13 individuals ranging in age from 1 to 130 months. Data on the age when trisomy 18 and trisomy 13 children achieved developmental skills were collected from a larger group of 62 trisomy 18 individuals and 14 trisomy 13 individuals whose families filled out parent questionnaires. Developmental quotient (DQ), defined as developmental age divided by chronological age, averaged 0.18 for trisomy 18 and 0.25 for trisomy 13. There was a dramatic drop in DQ from infancy to later childhood. The highest DQs and the greatest variation in DQs were in the first 2–3 years of life. Developmental ages in 7 skill areas were significantly different, with daily living and receptive language having the highest values and motor and communication skills having the lowest. When chronological age was taken into account, there was no significant difference in DQs in the same 7 skill areas, although there was a trend that was similar to the pattern of differences with developmental age. Older children could use a walker, understand words and phrases, use a few words and/or signs, crawl, follow simple commands, recognize and interact with others, and play independently. Walking and some toileting skills were also reported for trisomy 13. Although individuals with trisomy 18 and trisomy 13 were clearly functioning in the severe to profound developmentally handicapped range, they did achieve some psychomotor maturation and always continued to learn. © 1994 Wiley-Liss, Inc. 相似文献
19.
目的 观察表达白细胞介素18(IL-18)基因的条件增殖腺病毒在肾癌Ketr-3细胞中的生物活性及其对Ketr-3细胞的杀伤作用.方法 通过荧光显微镜观察表达绿色荧光蛋白的条件增殖腺病毒(ZD55-EGFP)在肾癌Ketr-3细胞中的感染和增殖情况.分别将表达IL-18的条件增殖腺病毒(ZD55-IL-18)及表达IL-18的普通腺病毒(Ad-IL-18)感染人肾癌Ketr-3细胞系,通过Western blot法检测病毒E1A和IL-18蛋白的表达;免疫细胞化学染色检测IL-18抗原表达;TUNEL法检测Ketr-3细胞的凋亡情况;噻唑蓝(MTT)比色法检测Ketr-3细胞存活情况.结果 ZD55-EGFP能有效感染肾癌Ketr-3细胞并在其中大量增殖.Westem blot检测结果 发现ZD55-IL-18能在肿瘤细胞内表达E1 A并有效介导IL-18表达,在病毒感染Ketr-3细胞48 h后,ZD55-IL-18、Ad-IL-18处理组的IL-18蛋白表达量分别为255.6±3.1、118.7±2.90免疫组织化学检测显示ZD55-IL-18、Ad-IL-18处理组的IL-18抗原阳性率分别为(82.4±3.2)%和(23.4±1.9)%.TNUEL检测结果 显示ZD55-IL-18、Ad-IL-18处理组的细胞凋亡率分别为(52.2±3.5)%和(25.5±1.9)%.病毒感染4 d后,MTT检测结果 显示ZD55-IL-18、Ad-IL-18处理组细胞存活率分别为(32.6±2.3)%和(73.3±2.5)%,表明ZD55-IL-18对Ketr-3细胞有显著的杀伤作用.结论 ZD55-IL-18能在Kerr-3细胞中高效特异性表达IL-18基因并显示出良好的抗肿瘤作用. 相似文献
20.
Rapid determination of trisomy 18 parental origin using fluorescent PCR and small tandem repeat markers: case reports 总被引:3,自引:0,他引:3
Ian Findlay Tamás Tóth Paul Matthews Tamás Marton Philip Quirke Zoltán Papp 《Clinical genetics》1998,53(2):92-95
Trisomy 18 is the second most common genetic defect after trisomy 21, almost 90% of which are due to additional chromosome from the mother. The parental origin of the additional chromosome can, if required, be determined by two methods: karyotyping, which takes several weeks; or, more recently, by polymerase chain reaction (PCR) which is often problematic. Fluorescent PCR of small tandem repeats (STRs) can determine the parental origin in the majority of cases within 5 h. Although the incidence of paternal origin is known for both trisomy 21 and trisomy 18, this technique can rapidly determine the parental origin in cases where there is insufficient samples to perform conventional tests. Determining parental origin by these methods may also have clinical significance in the diagnosis of chromosomal translocations or in the diagnosis of genetic disease using linkage analysis. 相似文献