高密度脂蛋白亚组份测定在预示冠心病中的意义

本文用沉淀法测定了54名正常人及51例冠心病人HDL-C及其亚组份HDL_2-C和HDL_3-C的水平。虽HDL-C含量在正常人和病人之间无差异,而HDL_2-C的水平表现为冠心病人(19.41±8.35mg/dl)明显低于正常人(25.15±8.66mg/dl),P<0.01.这项指标的总分辨率达62.9％,是测示冠心病较为敏感的指标。     

妊娠期肝内胆汁淤积症患者胎盘组织中有机阴离子转运多肽-A,-C表达的研究

目的 探讨有机阴离子转运多肽(organic anion transporting polypeptide,OATP)-A,-C在妊娠各期和妊娠期肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)患者胎盘的表达.方法 收集2006年5月至2007年2月,在本院产科确诊为肝内胆汁淤积患者纳入ICP组(n=20);同期在门诊手术室行终止妊娠术的正常早孕期妇女8例,在计划生育科采用米非司酮+米索前列醇或水囊引产的正常中孕期妇女7例及在产科手术室行剖宫产术正常分娩的晚孕期妇女20例,纳入对照组(n=35)(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该委员会批准,分组征得受试对象本人的知情同意,并与之签署临床研究知情同意书).两组胎盘组织中OATP-A mRNA,OATP-C mRNA测定,采用实时荧光定量PCR法;胎盘组织中有机阴离子转运多肽-A细胞学定位,采用免疫组织化学法.结果 ①对照组妊娠各期及ICP组胎盘组织中均检出OATP-A mRNA表达,均未发现OATP-C mRNA表达.对照组早、中、晚孕期胎盘组织中,OATP-A mRNA相对量比较,差异无显著意义(P>0.05);ICP组胎盘组织中,OATP-A mRNA相对量高于对照组(40.72±31.46 vs.21.54±15.65),差异有显著意义(P<0.05).②有机阴离子转运多肽-A在对照组晚孕期和ICP组胎盘的表达部位,均为滋养细胞,而在血管内皮细胞无表达.结论 胆盐载体OATP-A mRNA在妊娠期肝内胆汁淤积症患者的胎盘组织表达增高,可能是机体对抗胆汁淤积的一种反应.     

Proliferation kinetics of bone marrow cells in congenital dyserythropoietic anemia type II

Summary The proliferation kinetics of erythropoiesis and of myelopoiesis have been studied in a case of congenital dyserythropoietic anemia type II (HEMPAS) by means of quantitative 14-C autoradiography, Feulgen cytophotometry and 59-Fe ferrokinetics. Increased total erythropoietic activity and ineffective erythopoiesis was demonstrated by ferrokinetics. Quantitative 14-C autoradiography showed a generally delayed proliferation rate of erythroid cells, most evident in the polychromatic compartment. A deficiency of cell production of 25% was detected among the polychromatic erythroblasts. Part of this fraction is represented by cells still capable of passing to the successive stages of maturation. We conclude that only part of the deficiency of cell production in the polychromatic compartment represents real cell destruction. Most of the measured ineffectiveness is confined to later stages of maturation, such as orthochromatic erthroblasts and marrow reticulocytes.     

Mutual inhibition between olivary cell groups projecting to different cerebellar microzones in the cat

Summary Intracellular recording was made in the C3-C4 segments from cell bodies of a previously described system of propriospinal neurones (PNs), which receive convergent monosynaptic excitation from different higher motor centres and mediate disynaptic excitation and inhibition from them to forelimb motoneurones. Inhibitory effects in these PNs have now been investigated with electrical stimulation of higher motor centres and forelimb nerves. Short-latency IPSPs were evoked by volleys in the cortico-, rubro- and tectospinal tracts and from the reticular formation. Latency measurements showed that those IPSPs which required temporal summation were disynaptically mediated. After transection of the corticospinal tract in C2, only small and infrequent disynaptic IPSPs were evoked from the pyramid. It is postulated that disynaptic pyramidal IPSPs only to a small extent are evoked by monosynaptic excitation of reticulospinal inhibitory neurones known to project directly to the PNs, and that they are mainly mediated by inhibitory interneurones in the C3-C4 segments. Tests with spatial facilitation revealed monosynaptic excitatory convergence from tecto-, rubro- and probably also from reticulospinal fibres on inhibitory interneurones monosynaptically excited from corticospinal fibres (interneuronal system I). Disynaptic IPSPs were also evoked in the great majority of the PNs by volleys in forelimb muscle and skin nerves. A short train of volleys was usually required to evoke these IPSPs from group I muscle afferents. In the case of cutaneous nerves and mixed nerves single volleys were often effective, and the lack of temporal facilitation of IPSPs produced by a train of volleys showed strong linkage from these nerves. The results obtained after transection of the dorsal column at different levels show that the relay is almost entirely rostral to the forelimb segments. Test with spatial facilitation revealed that interneurones monosynaptically activated from forelimb afferents receive convergent excitation from corticospinal but not or only weakly so from tecto- or rubrospinal fibres. There was also convergence from group I muscle afferents and low threshold cutaneous afferents on common interneurones. It is postulated that the disynaptic IPSPs from forelimb afferents are mediated by inhibitory interneurones (interneuronal system II) other than those receiving convergent descending excitation. Volleys in corticospinal fibres, in addition to the disynaptic IPSPs, evoke late IPSPs in the PNs. Similar late IPSPs were evoked from the ipsilateral forelimb by stimulation of the FRA. Monosynaptic IPSPs were evoked in the majority of the PNs on weak stimulation of the lateral reticular nucleus (LRN) and from regions dorsal to it. Results from threshold mapping suggest that these IPSPs are due to antidromic stimulation of ascending inhibitory neurones which also project to the C3-C4 PNs, and that the ascending collaterals terminate in the LRN or/and the base of the cuneate nuclei. Activity in the ascending collaterals may give higher centres information regarding inhibitory control of the PNs. It is postulated that interneuronal system I subserves descending feed-forward inhibition and interneuronal system II feed-back inhibition from the forelimb of transmission through the C3-C4 PNs to motoneurones.This work was supported by the Swedish Medical Research Council (project no. 94)     

The effect of the D1-C785T polymorphism in the type 1 iodothyronine deiodinase gene on the circulating thyroid hormone levels in Romanian women with preeclampsia. Association with the degree of severity and pregnancy outcome of preeclampsia

Aim: To investigate the biochemical and genetic thyroid status in women with preeclampsia by the determination of serum FT3 and FT4 levels in association with D1-C785T genotypes. Methods: We genotyped using PCR–RFLP methods 50 women with preeclampsia and 50 normotensive pregnant women. Results: FT3 levels (pg/ml, 2.63?±?0.56 vs. 2.91?±?1.41) were low, and FT4 levels (ng/dl, 1.11?±?0.3 vs. 0.88?±?0.14) were high in women with preeclampsia compared to normal pregnant women. The association with severe preeclampsia was stronger for the homozygous T/T genotype (OR 6.57, p?=?0.029). Women with preeclampsia with the D1-T785 mutated allele had lower FT3 levels (pg/ml, 2.31?±?0.81 vs. 3.04?±?0.39, p?<?0.001), higher FT4 levels (ng/dl, 1.32?±?0.87 vs. 0.84?±?0.24, p?=?0.009) than women with preeclampsia with the D1-C/C genotype. Significant decrease in serum FT3 levels in positive women with severe preeclampsia compared to women negative for this genetic variation (pg/ml, 1.59?±?0.74 vs. 2.77?±?0.23, p?=?0.003) was observed. Women with severe preeclampsia, positive for the mutated T785 allele, delivered at a significantly lower gestational age (31.75?±?3.69 vs. 38.66?±?3.21 weeks, p?=?0.035) neonates with a lower birth weight (1861.11?±?869.9 vs. 3500?±?424.26?g, p?=?0.023) compared to women negative for the same allele. Conclusions: Thyroid hormone levels and the D1-C785T polymorphism, alone or in combination, correlate with the severity of preeclampsia. The D1-C785T polymorphism influences the outcome of pregnancy in severe preeclampsia.     

蜣螂中间体的大孔树脂纯化工艺优选及检测

目的:优选蜣螂提取物的DA201-C型大孔树脂纯化工艺.方法:采用单因素试验考察DA201-C型树脂的吸附性能和影响纯化工艺的pH、洗脱剂、上柱流速等因素;采用氨基酸自动分析仪检测纯化后的中间体.结果:最佳纯化工艺条件为上样液质量浓度17.60 g·L-1,吸附流速2 BV·h-1,pH 4.0,依次用1 BV水和4 BV 70％乙醇洗脱,脱盐率达98.81％,多肽回收率达87.39％,所得醇洗脱物含结合型氨基酸(多肽)32.26％.结论:该工艺分离效果较好,可用于蜣螂中间体的脱盐与纯化.     

肝内胆管结石与Apo AⅠ-CⅢ-AⅣ基因PstⅠ位点多态性的关系研究

目的 探讨载脂蛋白AⅠ-CⅢ-AⅣ(apolipoprotein AⅠ-CⅢ-AⅣ,Apo A Ⅰ-CⅢ-AⅣ)基因PstⅠ位点多态性与肝内胆管结石易感性(the happening of hepatolithiasis)的关系.方法 应用PCR和限制性内切酶技术对64例肝内胆管结石患者和120例正常人Apo A Ⅰ-CⅢ-AⅣ基因Pst Ⅰ位点进行限制性片段长度多态性(restriction fragment length polymorphisms,RFLP)分析.结果 肝内胆管结石组中A1A1、A1A2、A2A2基因型频率分别为0、4.7％、95.3％,正常对照组中A1A1、A1A2、A2A2基因型频率分别为0、10.0％、90.0％.肝内胆管结石组和正常对照组各基因型频率的比较(x2=1.599,P=0.206)和等位基因频率的比较(x2=1.531,P=0.216)差异均无统计学意义.结论 Apo A Ⅰ-CⅢ-AⅣ基因Pst Ⅰ位点多态性可能不是肝内胆管结石的危险因素.     

不同类型自身免疫性甲状腺疾病Th1/Th2平衡偏移的初步探讨

目的 初步探讨Th1/Th2平衡偏移在不同类型自身免疫性甲状腺疾病（AITD主要为Graves病和桥本甲状腺炎）中的作用机制和不同病程阶段的变化.方法 选取HT患者60例,GD患者60例,非毒性结节性甲状腺肿患者20例及健康体检者20例作为研究对象,各组间性别比例及年龄比较无统计学差异.通过ELISA法检测外周血清中Th1趋化因子（CXCL10）及Th2趋化因子（CCL22）的含量,比较各组间及不同病程阶段血清CXCL10、CCL22水平及二者（CXCL10/CCL22）比值有无差异;分析血清CXCL10、CCL22及二者比值与甲功（TT3、TT4、FT3、FT4、TSH）、甲状腺自身抗体（TPOAb、TGAb、TRA）及炎症因子（ESR、CRP）的相关性.结果 AITD患者血清CXCL10、CCL22水平及二者比值高于结节性甲状腺肿患者及正常体检者（P＜0.05）,后两者间差异无统计学意义.GD（疗程小于3个月者）患者血清CCL22水平明显高于HT者,而CXCL10/CCL22明显低于HT者（P＜0.05）,两组血清CXCL10水平比较,差异无统计学意义.初发AITD患者与复发者比较Th1/Th2趋化因子水平及比值差异无统计学意义,药物治疗稳定后,AITD患者血清趋化因子水平及比值有所下降,但仍高于正常体检者.血清CXCL10、CCL22水平及二者比值与TPOAb、TGAb、TRAb、CPR、ESR及甲状腺体积呈正相关,与FT3、FT4、TSH无相关性.结论 Th1及Th2共同参与了AITD的发病,但以Th1为主.HT与GD患者Th1/Th2偏移有所不同,后者Th2免疫异常更为显著,且经药物治疗后,GD患者Th1/Th2平衡恢复较HT者更为明显.