干豆腐掺人非食用色素地板黄的快速检验

干豆腐(豆皮、腐竹)是一种以大豆为原料制成的豆制食品。近年来,卫生检验部门发现有些地方干豆腐生产者为了增加豆皮色度赢得消费者的青睐,向豆皮中掺入涂饰家具用的成色品地板黄颜料,或同时加入天然食用色素姜黄,姜黄按照食品添加剂使用标准限量添加是安全的,而地板黄则属于非食用色素之类,它的主要成分是铬黄,含有大量的铬酸铅等有害物质,食用后对人体有害,是不允许向食品中添加的。为保护消费者利益,维护人体健康,对干豆腐掺入非食用色素地板黄的快速检验方法进行了初步研究,现报告如下。     

烧伤创面黄杆菌产β-内酰胺酶机制的研究

目的 :探讨黄杆菌临床分离多重耐药株产生 β 内酰胺酶的机制。方法 :质粒和染色体抽提以市售试剂盒进行 ;黄杆菌产 β 内酰胺酶用纸片法定性 ;质粒和染色体上 β 内酰胺酶基因的表达用PCR法 ;PCR产物进行基因测序和同源性比较。结果 :纸片法定性显示该菌产 β 内酰胺酶 ;经PCR和产物序列分析表明其染色体和质粒上均有 β 内酰胺酶PSE 1基因的表达。结论 :该多重耐药株对 β 内酰胺类抗生素的耐药性由染色体和质粒共同介导的PSE 1基因所决定。烧伤创面黄杆菌产β-内酰胺酶机制的研究@夏培元$第三军医大学第一附属医院国家药品临床研…     

犀黄合剂局部外用的动物实验研究

目的：探讨使用犀黄合剂在人为的动物口腔粘膜损害部位已形成溃疡后的组织变化。为临床治疗口腔粘膜溃疡提供研究资料。方法：以犀牛角粉、牛黄粉、碘甘油、地塞米松(简称犀黄合剂)制成酊剂，对运动损害部位进行试验，即将酊剂混悬液涂搽于家兔口腔粘膜已形成溃疡的损害部位，并在投药后的24、48、72、96、120小时分另4取该部位组织在光镜下进行组织变化观察。结果：发现涂药部位24、48小时内可见粘膜上皮呈连续性破坏，表层脱落坏死形成凹陷，溃疡底部结缔组织有大量淋巴细胞浸润，72小时后又出现多形核白细胞和浆细胞浸润，96小时后可见有肉芽组织增生和毛细血管网形成。大约120小时后局部溃疡面可见瘢痕组织达Ⅰ期愈合。结论：该合剂具有清凉解毒、消炎、消肿、腐蚀、烧灼，调节机体自身免疫机制。促进口腔粘膜溃疡的早期愈合和缩短病程，对临床上治疗口腔粘膜溃疡有一定的参考价值。     

中国医学

20060371益智合剂对模型鼠脑海马脂褐素和细胞凋亡的影响/邱南…∥中华老年多器官疾病杂志.-2005,4(4).-293益智合剂由熟地、酸枣仁、巴戟天等中药组成。取雄性Wister大鼠腹腔注射D-半乳糖造模。益智合剂小、中、大剂量组大鼠脑海马区切片PAS染色,脂褐素阳性细胞显著低于模型对     

黄药子醇提物不同处理方式对肝毒性的影响

目的:探讨不同极性黄药子醇提物组分导致肝毒性的强弱.方法:将黄药子75%乙醇提取物依次用氯仿、乙酸乙酯、正丁醇萃取,采用SD雄性大鼠进行体内筛选,观察不同部位肝毒性的大小,以血清酶学进行考查.结果:氯仿部位肝毒性作用最强,乙酸乙酯部位次之.结论:黄药子中弱极性化合物组分具有显著的肝毒性.     

Effect of curcumin on nitric oxide synthase expression in Iipopolysaccharide-activated microglia cells and the anti-oxidative effect of curcumin

BACKGROUND: It has been demonstrated that curcumin can increase the activities of various anti-oxidase in blood and tissue, effectively eliminate various free radicals, reduce the production of peroxisome, and alleviate oxidative stress reaction. Whether it has the same effect on microglia? OBJECTIVE: To observe the effects of curcumin on the expressions of inducible nitric oxide synthase (iNOS), nuclear factor-κB (NF-κB), and superoxide dismutase (SOD) in microglial cell line BV stimulated by lipopolysaccharide (LPS). DESIGN: An observational comparative study. SETTING: Research Room of Biochemistry, Medical College of Nantong University. MATERIALS: Mice microglia cell line BV, iNOS and NF-κB reporter gene plasmids were presented by Dr. Bhat.NR. from the Medical University of South Carolina (USA). Curcumin was produced by the Xi'an Branch of China Chengdu Scholar Bio-Tech. Co.,Ltd.; LPS (E.Coli O26:B6), anti-mice iNOS monoclonal antibody, horseradish peroxidase labeled goat-anti-mice IgG were the products of Sigma Company (USA). METHODS: The experiments were carried out in the Research Room of Biochemistry, Medical College of Nantong University from May 2006 to April 2007. ① Detection of iNOS: The cells were seeded onto 24-well plate at the density of 1×105, After the cells had adhered to the cover glasses, the cells were grouped as negative control group (the primary antibody was replaced by phosphate buffered solution PBS); normal control group (the cells were normally cultured); LPS-treated group (the cells were treated with LPS for 24 hours); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours). The expressions of iNOS protein were detected with immunocytochemical staining. ② Determination of iNOS and NF-κB gene activities: According to the introduction of the kit for transfection, iNOS or NF-κB report gene plasmids were transiently transfected with LipofectamineTM2000 liposomes into the cells in the 24-well plate for 24 hours. The cells were divided into normal control group (the cells were normally cultured after transfected with report gene plasmids); blank plasmid group (the cells were normally cultured after transfected with blank plasmids); LPS-treated group (the cells were treated with LPS for 4 hours after transfected with report gene plasmids); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours after transfected with report gene plasmids). The content of luciferase in the cell lysis buffer was determined after cell lysis. ③ Determination of SOD activity: The cells were seeded into culture bottle at the density of 1×106, and the divided into four groups, including normal control group (the cells were normally cultured); LPS-treated group (the cells were treated with LPS for 24 hours); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours); vitamin C+LPS group (the cells were treated with vitamin C for 1 hour and LPS for 24 hours). The SOD activity was determined with xanthine oxidase and quantitative colorimetric assay. MAIN OUTCOME MEASURES: The expressions of iNOS protein, iNOS and NF-κB, and the activity of SOD were observed. RESULTS: ① Expression of iNOS protein in microglia: The expression of iNOS protein in the LPS-treated group was obviously higher than that in the negative control group (P < 0.01); Those in the curcumin+LPS group were significantly decreased as compared with that in the LPS-treated group (P < 0.01). ② Expressions of iNOS and NF-κB genes: The expressions of iNOS and NF-κB genes in the LPS-treated group were significantly higher than those in the normal control group (P < 0.01); Those in the curcumin+LPS group were significantly lower than those in the LPS-treated group (P < 0.01). ③ SOD activity: The activity of SOD in the LPS-treated group was significantly lower than those in the normal control group (P < 0.01). It in the curcumin+LPS group and vitamin C +LPS group was significantly higher than that in the LPS-treated group (P < 0.01). CONCLUSION: Curcumin could inhibit the expression of iNOS in the activated microglia, and it also has the abilities in eliminating free radicals and antagonizing lipid peroxidation.