多巴胺对鱼精蛋白循环抑制作用的缓解

多巴胺对鱼精蛋白循环抑制作用的缓解伍建林＊谭洁芳＊陈秉学＊张建军＊本研究的目的是探讨缓解体外循环下心脏手术后鱼精蛋白中和肝素时心率减慢、血压下降作用的方法。资料与方法择期体外循环下心脏手术病人４５例，随机分为三组，每组１５例，采用安定－大剂量芬太尼麻...     

硫酸鱼精蛋白对产科DIC的治疗作用(摘要)

硫酸鱼精蛋白对产科ＤＩＣ的治疗作用（摘要）王福玲１戴淑真１赵洪国１９９２年３月～１９９５年４月，我们采用硫酸鱼精蛋白对４例羊水栓塞合并ＤＩＣ的病人进行了治疗，现报告如下。临床资料本组病人４例，年龄２５～２９岁。４例病人中，３例急性羊水栓塞行剖宫产，术...     

硫酸鱼精蛋白不同用药途径与不良反应之间的关系（附100例总结）

我院在2002年-2003年间对100例先天性心脏病和后天性瓣膜病合并肺动脉高压病人，在体外循环(CPB)后注射鱼精蛋白中和肝素时，分别采取经中心静脉导管注射用药和经主动脉根部注射用药，目的在观察两种用药途径与不良反应的关系，现报告如下。     

鱼精蛋白对牛主动脉平滑肌细胞增殖的影响

应用细胞培养新生牛主动脉平滑肌细胞传代培养，取第４－６代平滑肌细胞进行实验，采用细胞计数法观察不同浓度的鱼精蛋白以对牛主动脉平滑肌细胞增殖的影响。结果显示：不同浓度鱼精蛋白均能刺激牛主动脉平滑肌细胞增殖，鱼精蛋白的浓度与平滑肌细胞增殖量呈正相关。上述     

利用人工心肺机行自体血液回收的临床观察

目的探索自体血液回收的简便方法。方法利用人工心肺机单泵及贮血过滤配套装置将没有污染的胸、腹腔积血或手术切口渗血吸入贮血器,过滤并加入肝素后输入出血者体内。着重观察血液回收方法、抗凝、过滤及术后并发症等问题。结果观察组回收血量:550—6 200ml,平均(1 201.92±233.92)ml。观察组围术期输库存血量:0~800 ml,平均(292.3l±44.69)ml。而对照组使用库存血量600—3 000ml,平均(826.67±189.10)ml。观察组术后渗血量:50～680ml,平均(164.23±34.42)ml。与对照组无明显差别。观察组少数患者术后有一过性血红蛋白尿。观察组术后ACT均在正常范围。本组病例无术后脑栓塞并发症发生。结论 利用人工心肺机单泵及贮血过滤装置即可完成自体血液回收,该方法具有简单、经济、实用的优点,有一定推广应用价值。     

Analysis of the methylation pattern of six gene promoters in sperm of men with abnormal protamination

It has recently been shown that alteration of the methylation pattern of imprinted genes is associated with different types of male infertility. The objective of our study was to investigate the methylation pattern of selected gene promoters in sperm of patients with abnormal protamine replacement. The promoters of OCT4, SOX2, NANOG, HOXC11, miR-17 and CREM were analyzed using bisulfite sequencing and the percentage of DNA methylation was compared between patients with an abnormal protamine 1/protamine 2 (P1/P2) ratio and normozoospermic controls. No significant quantitative differences were found between groups of patients with either an abnormally high or low P1/P2 ratio compared to normal controls. However, two individual samples from infertile subjects (2/20, 10%) showed an altered methylation pattern for the CREM gene promoter that was not found in control samples. These two samples had a significantly higher (P<0.05) promoter methylation (5.58 and 4.23%, respectively) compared to the control group (0.46%). In conclusion, in our pilot study, extreme methylations defects were not seen broadly in severely infertile men. However, two patients exhibited altered methylation of the CREM gene, which may be either causative or a result of abnormal protmaine replacement.     

经升主动脉注射鱼精蛋白的肺保护作用

目的 探讨经升主动脉注射鱼精蛋白对婴幼儿体外循环心脏直视手术的肺保护作用.方法 60例(年龄≤1岁,体重≤10 kg)行体外循环心脏直视手术患儿,随机分为升主动脉组和中心静脉组各30例,比较2组注射鱼精蛋白前和注射鱼精蛋白后10 min、1 h、3 h气道峰压(Ppeak)、气道平台压(Pplate)及肺顺应性(CL)、氧合指数(OI)、肺隔离白细胞、中性粒细胞计数和术后呼吸机辅助通气时间的差异.结果 在注射鱼精蛋白1、3 h后,升主动脉组肺隔离白细胞、中性粒细胞计数、Ppeak、Pplate较中心静脉组明显减低(t值分别为2.743、3.512,3.218、3.469,3.716、5.243,3.853、4.783,P均＜0.05),升主动脉组CL及OI较中心静脉组明显增高(t值分别为3.976、4.267,4.557、4.265,P均＜0.05);升主动脉组较中心静脉组呼吸机辅助通气时间明显缩短[(8.03±5.14)、(10.56±6.95)h,t=2.599,P＜0.05].结论 经升主动脉注射鱼精蛋白能够减轻体婴幼儿体外循环心脏直视手术肺损伤,是一种良好的肺保护措施.

Abstract：

Objective To study the protective effect of intraaortic protamine injection on lung in infants undergwent opening heart operation by cardiopulmonary bypass surgery. Methods Sixty infants (age ≤ 1 year,weight ≤ 10 kg)who accepted opening heart operation by cardiopulmonary bypass surgery were randomly assigned into 2 groups ( n = 30 in each group) reciving intra-aortic and intra-venous protamine injection respectively. P-peak, P-plate, CL, Oxygenation Index, the number of WBC and neutrophil segregated in lungs were compared between two groups before injecting protamine and 10 minutes, 1 hour, 3 hours after injecting protamine. The time of mechanical ventilation were compared as well. Results P-peak, P-plate, the number of WBC and neutrophil segregated in lungs of intra-aortic injection group significantly decreased than intra-venous injection group at 1 hour, 3 hours after injecting protamine (t =2.743, 3.512; 3.218, 3.469; 3.716, 5.243; 3.853,4. 783 respectively, Ps ＜ 0. 05 ), while the CL and Oxygenation Index increased significantly ( t = 3. 976,4. 267; 4. 557,4. 265 respectively, P ＜ 0. 05 ). The duration of mechanical ventilation follow operation in intraaortic injection group ( [8. 03 ± 5. 14] h ) was shorter compared with intra-venous injection group ( [10. 56 ±6.95]h) (t =2.599,P＜0.05). Conclusion By intra-aortic protamine injection the lung injury decreased significantly. It shows good protective effect on lung in infants underwent opening heart operation by cardiopulmonary bypass surgery.     

地特胰岛素——一种用于治疗1型和2型糖尿病的长效胰岛素类似物

许多糖尿病患者对胰岛素治疗存在顾虑,担心注射带来不适、害怕发生低血糖以及体重增加,这给获得最佳血糖控制带来了挑战。基础胰岛素治疗对T2DM患者来说是一种有效且安全的方式。随着患者胰岛β细胞功能的进行性衰退,基础联合餐时速效胰岛素治疗成为了生理性胰岛素替代治疗的金标准方案。与中性鱼精蛋白锌胰岛素（NPH）相比,地特胰岛素（DET）治疗对体重增加影响较小和低血糖发生更少,是基础胰岛素治疗很好的选择。     

鱼精蛋白与特发性男性不育的研究进展

男性不育是一个较为复杂的临床综合征.WHO已明确指出不育症是指正常育龄夫妇12个月无保护性交而未能受孕. 近年来,越来越多的学者从遗传角度上对特发性男性不育患者进行分析.目前已经确定的遗传异常,包括染色体异常、精子mtRNA 突变,单/多基因遗传病以及遗传因素引起的内分泌异常等[2].从核酸与蛋白质水平上的进行了多角度的分析,其中鱼精蛋白的研究成为特发性男性不育症研究的一大热点.