深圳地区女性感染人乳头瘤病毒基因类型分析

目的了解深圳地区各年龄段与不同生殖道疾病女性感染人乳头瘤病毒(human papillomavirus,HPV)基因类型分布状况,为深圳地区HPV分子流行病学研究提供依据。方法利用反向杂交技术分别对2005年1月~2005年12月来医院就诊的801例可疑患者进行23种HPV基因亚型检测,其中包括18种高危亚型(HPV16,18,31,33,35,39,45,51,52,53,56,58,59,66,68,73,83,MM4)及5种低危亚型(HPV6,11,42,43,44)。结果HPV阳性检出率为17.5%(140/801),其中高、低危型检出率分别为16.9%(135/801)与2.6%(21/801),高危型中主要为HPV16,58感染,低危型中主要为HPV11感染。不同生殖道疾病(宫颈炎性病变、尖锐湿疣、宫颈上皮内瘤变(≥CIN))与正常对照组比较差异有显著性意义(P<0.05)。20~30 y,31~40 y,40~50 y和>50 y各年龄组中HPV阳性的比率为32%,38.5%,22.4%和7.1%。结论HPV11,16,58是深圳地区HPV感染的主要型别,20~50 y为感染的高峰人群,对HPV阳性者进行定期的跟踪,是防治尖锐湿疣与宫颈癌的重点。     

腺苷蛋氨酸联合熊去氧胆酸治疗淤胆型病毒性肝炎疗效观察

2000年1月～2003年5月,作者用腺苷蛋氨酸联合熊去氧胆酸治疗淤胆型病毒性肝炎,现将临床疗效报道如下:     

获得性血友病A致骨筋膜间隙综合征1例

<正>获得性血友病A是一种少见的出血性疾病,是因非血友病患者后天自身产生抗凝血因子Ⅷ抗体引起凝血功能障碍而致,其临床表现与典型血友病甲相似。国外统计,获得性血友病每年大约每10万人中有0.2~1.0人发病[1]。而因获得性血友病A局部出血导致的骨筋膜室综合征更为少见。1病例资料患者,女,32岁,因双小腿肿胀,疼痛,活动受限1 d来我院血管外科就诊。7天前患者曾因"上呼吸道感染"于当地     

留置尿管期间和拔管的护理进展

根据我国留置导尿操作进展，综述了留置尿管期间和拔管的护理，旨在为临床护理人员进行该项操作提供参考。     

Long-term survival of participants in the prostate ：ancer prevention trial

The Prostate Cancer Prevention Trial （PCPT） is a seminal study in the field of urology. More than 10years after its initial publication, updated data from this trial continue to shape our understanding of prostate cancer. Among the major findings from the PCPT has been the demonstration that prostate cancer is common in men with prostate-specific antigen （PSA） once thought to be in the normal range,~ finasteride prevents the development of benign prostatic hypertrophy,2 it increases the sensitivity of PSA3 and digital rectal examination.4 Furthermore the PCPT helped to establish the link between erectile dysfunction and cardiovascular disease,5 and perhaps most importantly finasteride demonstrated a 25% relative risk reduction in the diagnosis of prostate cancer compared with placebo.     

Immunodetection of Tau microtubule-associated protein in human sperm and testis

Dear Editor,
The cytosolic protein Tau is naturally present in human neurons, where it has a pivotal role in controlling microtubule stability. Hyperphosphorylation of Tau （observed during neurodegenerative diseases, such as Alzheimer＇s disease） impairs the protein＇s ability to bind microtubules. This results in microtubule disassembly and the formation of Tau aggregates, Tau protein is also widely expressed in peripheral tissues. In the male reproductive system, screening for Tau has focused solely on the rodent and bovine testis. In the present study, we used immunofluorescence and immunoenzymatic techniques （with a Tau-specific antibody） to investigate the presence of Tau protein in human ejaculated sperm and testicular tissue.     

Disposable circumcision suture device： clinical effect and patient satisfaction

In our experience patients undergoing circumcision are mostly concerned about pain and penile appearances. We conducted a prospective randomized trial to assess the benefits of a new disposable circumcision suture device （DCSD）. A total of 942 patients were equally divided into three groups （conventional circumcision, Shang ring and disposable suture device group）. Patients in the DCSD group were anesthetized with compound 5% lidocaine cream, the others with a 2% lidocaine penile block. Operation time, intra-operative blood loss, incision healing time, intra-operative and post-operative pain, the penile appearance and overall satisfaction degree were measured. Operation time and intra-operative blood loss were significantly lower in the Shang ring and suture device groups compared to the conventional group （P 〈 0.001）. Intra-operative pain was less in the suture device group compared With the other two groups （P 〈 0.001）; whereas post-operative pain was higher in the conventional group compared to the other two groups （P 〈 0.001）. Patients in the suture device （80.57%） and Shang ring （73,57%） groups were more satisfied with penile appearances compared with the conventional circumcision group （20.06%, P 〈 0.05）. Patients in suture device group also healed markedly faster than the conventional group （P 〈 0.01）. The overall satisfaction rate was better in the suture device group （78.66%） compared with the conventional （47.13%） and Shang ring （50.00%） groups （P 〈 0.05）. The combination of DCSD and lidocaine cream resulted in shorter operation and incision healing times, reduced intra-operative and post-operative pain and improved patient satisfaction with the cosmetic appearances.     

一次性1 ml注射器不同方法配制试验液时药物分布的对比研究

目的 :为探讨一次性 1ml注射器配制试验液时的最佳方法。方法 :采用 3种配制试验液的方法 ,每种方法配制试验液 10份 ,并分别对每份试验液的前 0 .5ml和后 0 .5ml的药物含量分布情况进行测试。结果 :第3种方法的药物分布情况及实际含量较为理想 ,误差最小。结论 :第 3种方法是可选的一种配制试验液的方法。     

巢式PCR检测庚型肝炎病毒感染

目的探讨逆转录-巢式PCR研究庚型肝炎病毒(HGV)感染在我国南方人群中的分布。方法从HGV 5-′NCR区段设计了内、外引物对,运用逆转录-巢式PCR对来自我国南方深圳、广州和桂林三个城市总共403例,且来自不同人群组的血清/血浆标本中HGV RNA感染率进行了研究。结果献血员人群中HGV RNA的平均感染率为5.8%(11/189),其中职业献血员较义务献血员高〔9.7%(10/102)及1.1%(1/87)〕;静脉药瘾者、丙型肝炎、乙型肝炎患者人群中HGV感染率依次为50.8%(33/65)、24.0%(9/37)、2.5%(1/40);在具有严重程度不同的非A-E肝脏疾病患者中HGV RNA阳性检出率为12.5%(9/22)。结论HGV与HCV合并感染极其常见,可能与其具有共同的传播途径有关。追踪临床资料,发现HGV的感染并未加重丙型肝炎患者的病情;多次静脉滴注药物、输血/输液、血液透析所致医院感染均是引起我国人群中HGV分布较高的重要危险传播因素;HGV的致病性较弱,可能不是引起非A-E肝脏疾病的主要致病因素。