实验性隐睾术后大鼠附睾的反应性变化

本研究描述了实验性隐睾术后大鼠附睾的形态学变化。结果表明,隐睾术后由于腹温作用和附皋管管腔内容物的改变,附睾管上皮细胞的形态和功能明显改变。术后早期上皮细胞出现无丝分裂相,上皮细胞的分泌和吞噬功能加强,胞质内含PAS阳性颗粒的细胞增多。随术后时间的延长,附睾上皮细胞变化逐渐趋于稳定。[     

超声及临床特征对附睾局灶性病变的鉴别价值

目的:评价超声及临床特征在附睾局灶性病变鉴别诊断中的应用价值。方法:回顾性分析附睾局灶性病变48例,包括非特异性附睾炎、附睾结核和附睾精液囊肿。分析附睾局灶性病变大小、形态、位置、回声强度及均一性,鞘膜腔是否有积液;病灶血流的程度以及患者年龄、病程和阴囊压痛程度。结果:附睾结核病灶平均面积比非特异性附睾炎病灶大(P<0.05),并多可见钙化斑。非特异性附睾炎病灶的血流信号比附睾结核(P<0.05)及附睾精液囊肿(P<0.01)丰富;非特异性附睾炎病程比附睾结核(P<0.01)和附睾精液囊肿(P<0.01)短;非特异性附睾炎病灶的压痛比附睾结核(P<0.01)和附睾精液囊肿(P<0.01)明显。附睾局灶性病变发生位置及有无鞘膜积液无明显区别(P>0.05)。结论:超声和临床特征对附睾局灶性病变鉴别诊断具有重要的价值,为临床诊断、治疗附睾疾病及评价疗效提供客观依据。     

昭通市常见蝇种名录及分布调查

本文报道昭通市蝇类调查结果。昭通市 80年代前尚无文献报道 ,为了解苍蝇的危害 ,提供科学防制资料。根据我市医学昆虫调查防治计划。我们于 1983年至 2 0 0 3年连续、断续在全市十县一 (区 )进行蝇类采集调查 ,现将结果报告如下 :     

温州市1995～2001年布鲁氏菌病监测

我市系乳品重点生产基地,上世纪80年代以前,由于引入乳牛不断从病畜中发现布鲁氏菌病(简称布病)并波及人群,1986年经省布病防治中心及畜牧部门共同调查后确定我市瓯海区和瑞安市为布病疫区,经反复查治后于1994年通过省级考核,达到了布病控制区标准。由于近年来我国西、北部地区出现再次流行,而我市的皮革工业非常发达,生的牛羊皮毛多数是从西部和北部牧区购入,为了防止该病的再次传入和巩固我市布病防制成果,根据上级方案要求,我市从1995～2001年对布病历史疫区和非疫区继续进行全面系统及重点人群的监测。现将监测结果分析如下。     

NK／LAK细胞对人口腔癌耐细胞药株杀伤活性的观察

OBJECTIVE: To understand the relation between cytotoxic activity of immunologic effector cells and multidrug resistance of the tumor cells. METHODS: Continuous observation of the morphological changes and MTT colorimetry were employed to evaluate the cytotoxic activity of lymphokine-activated killer (LAK) cells and natural killer (NK) cells against multidrug-resistant (MDR) human oral carcinoma cell line-KBV200 (before and after reversal of MDR) and parental drug-sensitive cell line KB. The morphologic changes of LAK cells and the 3 target cell lines were observed continuously under inverted microscope 3 h after co-culture of LAK cells with one of three target cell lines respectively. The lysis rates of three tumor cell lines in response to co-culture with LAK or NK cells were determined using MTT colorimetry. RESULTS: In comparison with the parental drug-sensitive cell line KB, both KBV200 and its reserved cell line by verapamil (KBVV) showed earlier adherence and greater number of cells lysed by LAK. In MTT colorimetry assay, the cytotoxicity of both LAK and NK cells against the 3 cell lines was associated with the effector-to-target (E/T) cell ratio; the lysis rates of KBV200 and reversed KBV200 cells by verapamil in response to LAK and NK cells were higher than that of KB cells (P<0.05), but KBV200 and KBVV did not significantly differ (P>0.05). At the same E/T ratio, LAK cells possessed stronger cytotoxicity than NK cells against all the tumor cell lines (P<0.05). CONCLUSIONS: Immunologic effector cells possess strong cytotoxic activity against multidrug-resistant cell line KBV200. Modulation of MDR does not decrease the cytotoxic activity of the immunologic effector cells. The results of this study suggest that adoptive cell immunotherapy with immunologic effector cells may be of value in controlling the progress of MDR tumors.     

真菌病

20 0 4 0 779　唑类药物体外诱导白念珠菌耐药的初步研究/宋晓静(浙大一附院皮肤科)…∥临床皮肤科杂志.- 2 0 0 3,32 (10 ) .- 575～576将质控株及敏感白念珠菌在含不同抑菌浓度的氟康唑(FCZ)、酮康唑(K CZ)、伊曲康唑(ICZ)的液体培养基中进行人工传代诱导耐药。结果白念珠菌在高浓度的唑类药物培养基中能诱导耐药,且为交叉耐药,而在低浓度中经传2 0代仍不能诱导耐药,诱导后的耐药菌株在无药物培养基中传代可恢复敏感性。提示唑类药物能诱导耐药白念珠菌的产生,但与诱导药物的浓度有关,且为不稳定耐药。(汤亚娥)2 0 0 4 0 780　白念珠…     

艾滋病病毒出现新变种

有的喀麦隆人显示出艾滋病的症状，但艾滋病病毒的测试结果却为阴性。     

骨质疏松症动物模型

动物模型在骨质疏松症的研究中广为采用，目前常用的动物有大鼠、小鼠、狗、小型猪、绵羊、兔、灵长目等，各有优点和缺点。研究者应根据实验要求和目的，慎重选择。常用的造模方法有去势、药物诱导和制动致骨质疏松症，最后通过多种指标综合分析，实现对骨质疏松症动物模型的全面判断。