螺旋神经节神经元原代培养及其免疫细胞化学鉴定

目的 建立成体哺乳动物耳蜗螺旋神经节神经元（ＳＧＮ）体外培养的细胞模型。方法 对出生后７ｄ的Ｗｉｓｔａｒ大鼠的螺旋神经节细胞进行解离与分散培养。用荧光显微镜与倒置相差显微镜观察原代培养ＳＧＮ细胞的活力以及生长与分化过程。应用链霉卵白素过氧化物酶（ＳＰ）方法和抗神经丝蛋白单克隆抗体（ＮＦＰ－ＭｃＡｂ）对ＳＧＮ进行免疫细胞化学染色鉴定。结果 幼龄Ｗｉｓｔａｒ大鼠的ＳＧＮ在体外条件下，可良好存活并有正常     

血清sVCAM—1检测对肝硬化患者的临床意义

目的：探讨血清可溶性血管细胞粘附分子－1（sVCAM-1)在肝硬化发生发展中的作用及临床意义。方法：以双抗体夹心ELISA法测定肝硬化患的血清sVCAM-1水平。结果：（1）肝硬化组的血清sVCAM-1水平明显高于正常对照组（P＜0．01）；（2）肝硬化患血清sVCAM-1水平随肝功能分级增加而升高。结论：血清sVCAM-1水平可反映肝硬化损害程度和炎症活动性，对其进行动态观察可对病情作出有价值的评估。     

心肌局部肾素—血管紧张素系统的研究进展

自1898年 Tigersted、Bergmam 首次发现肾素以来,血浆肾素—血管紧张素系统的生化链及其有关酶介导的肽类激素的形成已基本清楚。这一系统在健康及疾病状态下所起的作用,加深了人们对其生理及病理意义的理解。60年代中期,有人首先报告了心肌的血管紧张素效应,随后许多研究者相继发现这一肽类激素对心脏的直接及间接作用。由于第二信使学说的建立,奠定了阐明这一肽类激素效应的基础,而广泛的血管紧张素转换酶抑制剂的应用     

大鼠全脑缺血再灌后几个脑区在不同时间的LPO,SOD,GSH…

本文采用四血管阻断方法制成Ｗｉｓｔａｒ大鼠全因１０分钟再灌流模型。分别于再灌流７天内对新皮层，海马，丘脑，纹状体和小脑等五个脑区的ＬＰＯ、ＳＯＤ、ＧＳＨ－Ｐｘ含量进行测定。ＬＰＯ在各脑区于再灌流后开始升高，２４小时达高峰，第５天基本降至对照组水平。ＳＯＤ在６小时内迅速降至最低水平，然后逐渐回升，但在第７天时仍低于对照水平，ＧＳＨ－Ｐｘ在各脑区均呈双时相改变，先降后升，然后再逐渐下降，在第７天时略低     

Functional Maturation of Neuroendocrine Gonadal Axis is Altered by Specific Phase Relations of Circadian Neurotransmitter Activity in Japanese Quail

The preset study was designed to ascertain the effects of temporal relationship of circadian neural oscillations on puberty attainment and reproductive growth of Japances Quail,Coturnix coturnix japonica,Serotonin and dopamine precursors(5-hydroxytryptophan,5-HTP and L-dihydroxyphenylalanine,L-DOPA,5mg/100g body weight)were injected daily,8and 12h apart in two groups of one-day ld chick,while cotrols received two daily injections of normal saline.Weekly/biweekly observations(body weight,cloacal gland size,testicular volume and activity,ovarian follicular diameter and rate of egg production)were made until 9 weeks of age,when the experiment was terminated.Results indicate that 8 h relationship completely suppressed gonadal growth even under long photoperiod(LD16:8),while a 12 h relationship induced precocious sexual maturity and increased the rate of reproductin(spermatogenesis and egg production).It is concluded that circadian phase relationship of serotonergic and dopaminergic activity may not only determine the onset of reproduction in this pultry species,but may also alter the rate of reproduction possibly by affecting photoperiodic mechanism of reproductive regulation.