重组质粒pSecTag2/B-OPG在Beagle犬骨髓基质细胞中表达与鉴定

目的转染骨保护素（OPG）基因至Beagle犬骨髓基质细胞（BMSCs）中,并检测其表达能力,为基因工程技术治疗牙周病提供物质基础。方法穿刺抽取成年Beagle犬骨髓2～3ml,通过全骨髓贴壁法获取BMSCs。传代至第3代,脂质体瞬时转染经鉴定的重组pSecTag2／B-OPG,并用RTPCR、Western blot检测OPG的表达水平。结果重组质粒pSecTag2／B-OPG经HindⅢ单酶切及EcoRⅠ、BamHⅠ双酶切,电泳显示切下的片段均与预期大小相符,经测序证实此基因与GenBank中提供的序列[gi：33873056]一致;RTPCR、Western blot结果显示：重组pSecTag2／B-OPG在骨髓基质细胞中有表达。结论成功建立了OPG基因修饰的骨髓基质细胞瞬时基因表达体系。     

仿生种植牙模型的三维有限元研究

目的 研究仿生种植牙、天然牙和骨性结合种植体3种模型骨界面上的应力分布,从理论上探讨种植体的新模型.方法 应用螺旋CT对上颌中切牙标本进行扫描,分别建立仿生种植牙、天然牙和骨性结合种植体模型,对3个模型施加同样的载荷,应用Super SAP93软件,计算出天然牙周膜和仿生牙周膜以及3种模型骨界面上的应力分布.结果 仿生牙周膜和天然牙周膜内外表面应力的变化趋势相同;骨性结合种植体模型骨界面上在颈部出现应力集中;仿生种植牙和天然牙2个模型骨界面上最大Von Mises应力从颈部到根端的变化比较均匀,没有出现颈部应力集中现象.结论 引入仿生牙周膜能起到天然牙周膜的生物力学功能,它能实现牙合力的分散和减缓作用,使仿生种植牙骨界面上的应力分布比较均匀,从生物力学相容性上说明了仿生种植牙模型的可行性.     

携带IL-1B等位基因2牙周炎患者基础治疗后牙周可疑致病菌的变化

 【目的】了解携带IL-1B（＋3954）等位基因2的牙周炎患者在牙周基础治疗后龈下菌斑中牙周可疑致病菌的动态变化情况。【方法】选择了携带IL-1B（＋3954）等位基因2牙周炎患者的42颗患牙为实验组（B2＋group）,无携带该等位基因牙周炎患者的40颗患牙为对照组（B2-group）。收集两组患牙在牙周基础治疗前、治疗后1、3、6个月时牙周临床指标菌斑指数（PI）、牙龈指数（GI）和探诊深度（PD）,采集龈下菌斑,采用细菌培养鉴定方法测定牙周可疑致病菌的检出率和检出量（CFU/g）。【结果】（1）在同一观察时间点,两组的临床牙周各项指标无统计学差异。（2）基线时,实验组和对照组的牙周可疑致病菌检出率无统计学差异,而实验组的梭杆菌、类杆菌和BPAR检出量则较对照组高（P〈0.05）。（3）基础治疗后1个月,两组的检出率和检出量明显下降（P〈0.05）。（4）治疗后3个月时,两组的可疑致病菌检出率和检出量较1个月均有上升,且实验组的BPAR检出率和检出量均较对照组高（P〈0.05）。（5）治疗后6个月时,两组的检出率基本回复基线水平;而实验组的真杆菌、梭杆菌、类杆菌和BPAR检出量则较对照组高（P〈0.05）. 【结论】（1）对于PD≥6mm的牙周炎患牙，基础治疗只能暂时控制牙周可疑致病菌。（2）基础治疗前后，携带IL-1B（＋3954）等位基因2患者的牙周深袋处都可检出有更多的梭杆菌、类杆菌和BPAR。     

牙周牙髓联合病变180例临床治疗

目的观察牙周牙髓联合病变的治疗效果。方法180例牙周牙髓联合病变患者,对其进行临床对症治疗。结果观察大部分患牙得以保留。结论临床医生对症治疗和患者良好配合可取得良好效果。     

茶多酚对人牙周膜成纤维细胞增殖的影响

目的 观察茶多酚对人牙周膜成纤维细胞增殖的影响.方法 采用组织块培养法培养原代人牙周膜成纤维细胞并传代,经免疫组化SABC法检测角蛋白和波形丝蛋白鉴定,取5～8代细胞用于实验;按茶多酚不同浓度分1 mg/ml(B组)、0.5mg/ml(C组)、0.25mg/ml(D组)、0.125mg/ml(E组)、0.0625mg/ml(F组)组和空白对照组(A组),采用细胞计数法、MTT法检测细胞增殖情况,流式细胞术检测细胞DNA含量.结果 不同浓度茶多酚均促进人牙周膜成纤维细胞的增殖和DNA合成(P＜0.05),茶多酚作用的最适浓度为0.5 mg/ml,最佳作用时间为48 h.结论 茶多酚对人牙周膜成纤维细胞增殖具有明显的促进作用.     

炎症状态下人牙周膜成纤维细胞中SLC7A11和GPX4的表达水平及其意义

目的:探讨在牙龈卟啉单胞菌脂多糖(P.g-LPS)刺激的炎症环境下,人牙周膜成纤维细胞(hPDLFs)中溶质载体家族7成员11 (SLC7A11)和谷胱甘肽过氧化物酶4 (GPX4)表达的变化,阐明P.g-LPS诱导牙周炎的可能机制。方法:体外提取并培养hPDLFs,采用免疫组织化学染色进行鉴定。hPDLFs分为对照组(0 mg·L^-1P.g-LPS)、1 mg·L^-1P.g-LPS组和10 mg·L^-1P.g-LPS组,处理24 h。采用实时荧光定量PCR (RT-qPCR)法检测各组细胞中肿瘤坏死因子α (TNF-α)、白细胞介素6 (IL-6)、白细胞介素1β (IL-1β)、 SLC7A11和GPX4 mRNA表达水平,Western blotting法检测各组细胞中SLC7A11和GPX4蛋白表达水平,2’, 7’-二氯荧光素二乙酸酯(DCFH-DA)荧光探针法检测各组细胞中活性氧(ROS)水平。结果:RT-qPCR法检测,与对照组比较,1和10 mg·L^-1P.g-LPS组细胞中TNF-...     

神经生长因子对人牙周膜细胞增殖与分化作用的研究

目的 研究神经生长因子对体外培养的人牙周膜细胞增殖和分化作用的影响.方法 采用四唑盐比色法(MTT)和碱性磷酸酶(ALP)活性检测法,测定不同浓度(0.1 U/ml、1 U/ml、10 U/ml、100 U/ml)的神经生长因子对体外培养的第5-6代人牙周膜细胞增殖及碱性磷酸酶活性的作用.结果 与对照组(2?S的DMEM培养液)相比,1,10,100 U/ml的神经生长因子都可促进人牙周膜细胞的增殖,但10 U/ml作用最显著;100 U/ml的神经生长因子可显著促进碱性磷酸酶的活性(P＜0.01).结论 不同浓度的神经生长因子可显著促进人牙周膜细胞的增殖与分化.     

Tooth and periodontal clinical attachment loss are associated with hyperglycemia in patients with diabetes

Background: Periodontal disease has been associated with diabetes, but there is still controversy on the relationship between periodontal clinical parameters and glycemic control. The purpose of this study is to assess the relationship between blood glucose levels and clinical parameters of periodontal disease in individuals with diabetes. Methods: A total of 65 individuals with diabetes and 81 individuals without diabetes were included in the study. A full-mouth periodontal examination and preprandial fasting glycemia values were recorded for each individual. Glycosylated hemoglobin was only measured in patients with diabetes. A comparative analysis between groups (Mann-Whitney U test) and a correlation analysis between glycemia and periodontal parameters were performed (Spearman test). Results: Patients without diabetes presented more teeth than individuals with diabetes (P <0.05). Patients with diabetes with periodontitis displayed loss of periodontal clinical attachment compared to patients without diabetes, but the highest value was observed in patients with periodontitis that reported a smoking habit. Furthermore, patients with diabetes with periodontitis presented higher glycemia and glycated hemoglobin values in contrast to patients with gingivitis. Patients with diabetes with hyperglycemia had a higher risk to develop periodontitis (odds ratio = 2.24; 95% confidence interval = 1.02 to 4.93). A positive correlation was observed between glycemia and clinical attachment loss (AL), whereas a negative correlation between glycemia and the number of teeth present was found (P <0.05). Conclusions: Tooth and periodontal AL were increased by hyperglycemia in individuals with diabetes. This study contributes additional evidence that diabetes could aggravate periodontal disease and affect the systemic health of individuals.     

Sickle cell disease does not predispose to caries or periodontal disease

This study investigated the prevalence of dental caries and periodontal condition in a population with sickle cell disease (SCD), analyzing some associations with disease severity. The Decayed, Missing and Filled Teeth index (DMFT) and Community Periodontal Index (CPI) were recorded for 99 individuals with SCD and 91 matched controls. Socio-demographic status, oral health behaviors, and history of clinical severity of SCD were assessed. Statistical comparisons were performed between the group with SCD and the control group, as well as multivariate logistic regression analyses with DMFT index and CPI as the dependent variables. The mean number of decayed teeth was significantly higher in individuals with HbSS. Older age, female gender, and daily smoking were identified as risk factors for higher DMFT, while older age and absence of daily use of dental floss were risk factors for the development of periodontal disease. In conclusion, risk factors known to cause caries and periodontal disease had more influence on oral health than the direct impact of SCD.     

Periodontal findings in a patient with Mauriac syndrome: a case report

Mauriac syndrome is a rare condition that affects people with uncontrolled diabetes. The case history of a 17-year-old female patient diagnosed with Mauriac syndrome and concurrently suffering from type 1 diabetes mellitus (T1DM), stunted growth, hepatomegaly, and severe periodontal disease is described. Non-surgical periodontal therapy was the treatment provided. Severe periodontal destruction was seen in conjunction with the unique features of this syndrome, such as hepatomegaly, dwarfism, moon-shaped face, cushingoid fat deposition, delayed puberty, and brittle diabetes. Treatment resulted in decreased plaque and gingival scores. A bi-directional relationship has been established between diabetes mellitus and periodontal disease and there is an increasing emphasis on perio-systemic interrelationships. Given the effect that such systemic disorders have on periodontal health, periodontists should be involved in the early diagnosis and treatment of such patients.