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51.
目的 观察LeftyA蛋白对转化生长因子β1(TGF-β1)诱导人肾小管上皮细胞(HK-2)纤维化的影响,并初步探讨其作用机制.方法 体外培养人肾小管上皮细胞(HK-2)细胞,通过LipofectamineTM2000介导基因转染及药物筛选构建LeftyA蛋白稳定表达HK-2细胞系;TGF-β1(10ng/ml)刺激此细胞系,于0、6、12、24、48h通过Western blot分别检测E-钙粘蛋白(E-cadherin)、α-平滑肌肌动蛋白(α-SMA)及p-Smad2/3的表达变化,通过Real-time PCR检测Ⅰ型胶原(ColⅠ)及结缔组织生长因子(CTGF)基因的表达变化.结果 不同浓度的TGF-β1刺激24 h后HK-2细胞内E-eadherin、α-SMA蛋白的变化均表现出明显的剂量效应,而当TGF-β1浓度达到10ng/ml时,α-SMA蛋白合成达到平台期(P>0.01),因此本实验中以10ng/ml的TGF-β1作为刺激剂量;TGF-β1(10 ng/ml)刺激HK-2细胞6 h可引起E-cadherin表达下降(P<0.01),刺激24 h后可诱导其合成表达α-SMA(P<0.05),同时细胞内ColⅠ基因转录增加(P<0.01);正常HK-2细胞内没有p-Smad2/3的表达,TGF-β1(10 ng/ml)刺激30min即可诱导其激活表达,并在1 h时迅速达到高峰,其后开始下降;高表达LeftyA蛋白能够减轻TGF-β1所致上皮细胞转分化(EMT)及纤维化的程度,降低p-Smad2/3的表达高峰,并抑制CTGF的表达(P<0.01).结论 LeftyA蛋白能够通过抑制TGF-β1所致Smad2/3磷酸化及CTGF表达发挥抗肾小管上皮细胞纤维化的作用. 相似文献
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One hundred and twenty-five cases of acute myeloid leukemia (AML) were reviewed for the presence of Auer rods by two peroxidase methods. The percentage of Auer rods recognized by Wright-Giemsa (WG) staining was 20.8% but three times higher by peroxidase staining techniques using either benzidine base as a substrate (PO method) or 3,3'-diaminobenzidine as a substrate (DAB method). Both PO and DAB methods were equally sensitive in identifying Auer rods, the higher percentage being in FAB types M2 and M4 and lower in M1, M3, and M5B, with none in M5A. Cell counts of at least 100 cells were as accurate as counting cells up to a total of 1000 consecutive blasts. 相似文献
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In severe congenital scoliosis, traction (whether with a halo or instrumental) is known to expose patients to neurologic
complications. However, patients with restrictive lung disease may benefit from halo traction during the course of the surgical
treatment. The goal of treatment of such deformities is, therefore, twofold: improvement of the respiratory function and avoidance
of any neurologic complications. We report our technique to treat a 17-year-old girl with a multi-operated congenital scoliosis
of 145 ° and cor pulmonale. Pre-operative halo gravity traction improved her vital capacity from 560 cc to 700 cc, but led
to mild neurologic symptoms (clonus in the legs). To avoid further neurologic compromise, her first surgery consisted of posterior
osteotomies and the implantation of two sliding rods connected to loose dominoes without any attempt at correction. Correction
was then achieved over a 3-week period with a halofemoral traction. This allowed the two rods to slide while the neurologic
status of the patient was monitored. Her definitive surgery consisted of locking the dominoes and the application of a contralateral
rod. Satisfactory outcome was achieved for both correction of the deformity (without neurologic sequels) and improvement of
her pulmonary function (1200 cc at 2 years). This technique using sliding rods in combination with halofemoral traction can
be useful in high-risk, very severe congenital scoliosis.
Received: 6 January 1999 Accepted: 12 April 1999 相似文献
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The primary visual cortex of the tree shrew is characterized by the lack of ocular dominance columns. The two eyes are represented in sublayers of laminae 3 and 4. In an earlier study using the transneuronal transport we observed that the geniculate afferents from the two eyes do not initially overlap and then segregate into their appropriate sublaminae. The final distribution pattern can already be observed during the early postnatal period. Since segregation and elimination of afferent terminal branches do not seem to take place, we wanted to investigate whether or not an overproduction of synapses can be observed as in several other animals. We examined layers 3B, 3C, 4A, and 4B, which receive afferents from the retina via the lateral geniculate nucleus, from P5 to maturity by using the electron microscope. The brain tissue was excised in the region where the central vision is represented in adult animals. Then we determined the density of synapses per 100 microns 2 neuropil for each of the four sublayers at the ages P5, P15, P19, P23, P31, and P42 and in the adult animal (AD). In determining the neuropil we measured the size of two additional compartments, i.e., the compartments consisting of perikarya and of blood vessels. At a higher resolution we determined the fraction of Gray type I and type II synapses in each sublamina and in each developmental stage. The size of the neuropil increases from 57% at P5 to 81% in AD whereas the compartment of perikarya decreases from 42% to 15% and the compartment of blood vessels increases from 1.3% to 3.9%. The synaptic density starts with very low values (3.5/100 microns 2) at P5. Then it increases rapidly and attains a maximal rate of increase during the period of eyelid opening. After this period the increase is slowed down and approaches the adult value (12.5/100 microns 2) slowly. An overproduction of synapses could not be observed. The percentage of type I and type II synapses also changes during this period. The fraction of type I synapses amounts to 73% at P5 and increases to 92% in AD. The increase in density of type I synapses is continuous and does not show any sign of overproduction. The density of type II synapses rapidly reaches it final value and then remains constant. Possibly there is a slight overproduction during the period of eyelid opening.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
58.
Young‐Eun Park MD PHD Jin‐Hong Shin MD PhD Boram Kang MS Chang‐Hoon Lee MD PhD Dae‐Seong Kim MD PhD 《Muscle & nerve》2016,53(3):479-484
Introduction: Mutations in the gene encoding nebulin (NEB) are known to cause several types of congenital myopathy including recessive nemaline myopathy and distal nebulin myopathy. Core‐rod myopathy has recently been reported to be another type of NEB‐related myopathy, and is pathologically characterized by the coexistence of cores and nemaline rods within muscle fibers. Methods: We describe 2 patients with core‐rod myopathy who were analyzed genetically by whole exome sequencing and evaluated clinically and pathologically. Findings were compared with those of patients with the disease of other genetic causes. Results: Three NEB mutations were identified, 2 of which were novel. Mild clinical features, unusual patterns of muscle involvement, and atypical pathological findings were observed. Conclusions: We propose that the clinical and pathological spectrum of core‐rod myopathy should be widened. A significant amount of residual nebulin expression is believed to contribute to the much milder phenotype exhibited by the patients we describe here. Muscle Nerve 53: 479–484, 2016 相似文献
59.
目的研究明胶酶(MMP-2,9)与转化生长因子(TGF-β1)及其Ⅰ型受体(TGF-β1RⅠ)在大肠癌组织中的蛋白表达及其相互关系。方法应用免疫组织化学S-P法检测89例大肠癌组织中MMP-2、MMP-9与TGF-β1、TGF-β1RⅠ蛋白的表达。结果大肠癌MMP-2、MMP-9、TGF-β1和TGF-β1RⅠ阳性率分别为67.4%、65.2%、85.4%和71.9%;MMP-2表达与肿瘤大小和淋巴结转移均呈正相关(均P〈0.01),MMP-9表达与淋巴结转移呈正相关(P〈0.01),TGF-β1RⅠ的表达与淋巴结转移呈负相关(P〈0.01);TGF-β1表达状况与MMP-2和MMP-9的表达均呈显著正相关(r=0.256和0.365,P〈0.05和0.001),TGF-β1RI和MMP-9的表达呈显著正相关(r=0.225,P〈0.05)。结论MMP-2,9的表达情况与大肠癌侵袭转移有密切关系。MMP-2,9的表达与TGF—β1、TGF-β1RI关系密切,可能在一定程度上受到TGF-β1及其受体的调控。 相似文献
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