新型骨替代材料硅磷酸钙的生物相容性实验研究

目的:评价新型骨替代材料硅磷酸钙的生物相容性,从而为其在骨缺损修复领域中的临床应用提供依据。方法:分别选用含10%、5%硅酸钙的硅磷酸钙,分别进行急性毒性试验、肌肉植入试验。结果:含10%与5%硅酸钙的硅磷酸钙无急性全身毒性,对肌肉组织无刺激,植入肌肉后呈降解趋势。结论:新型骨替代材料硅磷酸钙具有良好的生物相容性。     

前交叉韧带重建术后短期内移植体黏性变化

目的 研究前交叉韧带重建(anterior cruciate ligament reconstruction, ACLR)术后短时间内移植体黏性的变化规律。方法 选择6只雄性新西兰兔,以跟腱为移植体制作单侧膝关节ACLR动物模型。ACLR术后15 d将实验兔安乐死,取出移植体、健康的前交叉韧带(anterior cruciate ligament, ACL)和跟腱。测量移植体的截面积,分别进行0.1、1 MPa平衡条件的蠕变实验,并计算黏性系数,总结移植体的黏性变化规律,与健康ACL进行对比。结果 移植体的截面积在手术后15 d内缓慢上升。ACL与移植体的黏性呈非线性变化。在不同应力下,黏性系数有较大差异。移植体黏性系数随ACLR术后时间呈下降趋势,但在低应力下更明显。结论 本文建议,ACLR术后早期康复过程中,应使用助行器,降低步频,并避免踢、踹等对关节冲击的运动。     

Fibrin glue assisted amniotic membrane graft reconstruction of conjunctival fornix and canthus following excision of conjunctival tumours

Conjunctival tumors involving non-limbal locations, such as the fornix and canthus, are typically excised using a “non-touch” technique, often with a wide surgical margin. Reconstruction of these large defects can be difficult due to the contour of the ocular surface and are often complicated by shortening of the fornix, symblepharon formation, and restriction of eye movements. In our experience, the use of amniotic membrane grafts combined with the sealant properties of fibrin glue such as Tisseel® has improved our surgical outcomes during the reconstruction phase. We would like to highlight and describe our surgical technique using fibrin glue and squint hooks to aid amniotic membrane graft reconstruction for surgically challenging locations in the fornix and canthus following excision of conjunctival lesions, with excellent surgical outcomes.     

Use of Gamma-Irradiated Cornea to Plug Sclerostomy Site During Tube Shunt Revision

Tube shunt implantation is a common procedure for control of intraocular pressure (IOP). However, tube revision and repositioning must sometimes be performed, and this involves removing the tube from its sclerostomy site. This site is prone to leaking and this may cause postoperative hypotony. We describe a novel and cosmetically acceptable technique of plugging and covering the sclerostomy site with gamma-irradiated corneal tissue.     

氟伐他汀对慢性移植物抗宿主病狼疮样肾炎小鼠肾组织MMP-9/TIMP-1表达的影响

目的观察氟伐他汀对慢性移植物抗宿主病(cGVHD)狼疮样肾炎小鼠肾组织中基质金属蛋白酶9(MMP-9)、金属蛋白酶1组织抑制剂(TIMP-1)表达的干预作用,探讨氟伐他汀对肾脏的保护机制。方法B6D2F1代杂交鼠随机分为3组(均n=6):正常对照组、模型组和氟伐他汀组。制作狼疮样肾炎小鼠模型后,氟伐他汀组每只小鼠予氟伐他汀0.22 mg灌胃,模型组每只予等量生理盐水灌胃。双缩脲法测定24 h尿蛋白量;免疫组织化学染色法及逆转录-多聚酶链反应(RT-PCR)法观察肾组织中MMP-9、TIMP-1表达;明胶酶谱法检测MMP-9活性。结果与正常组相比,模型组小鼠24 h尿蛋白量、MMP-9、TIMP-1表达及比值均增加,MMP-9活性增强,且与系膜细胞增殖呈正相关;与模型组相比,氟伐他汀组尿蛋白量、MMP-9、TIMP-1表达及比值均降低,MMP-9活性下降。结论氟伐他汀可能通过降低MMP-9、TIMP-1表达及MMP-9活性,改善MMP-9/TIMP-1失衡,从而发挥肾脏保护作用。     

树突状细胞的免疫耐受性及其在抗移植排斥中的应用

树突状细胞(dendritic cell,DC)作为专职抗原递呈细胞,其强大的抗原递呈以及免疫调节能力越来越引起人们的重视.近年来认为,DC具有调控免疫应答的作用,不仅可激发免疫反应,也可介导免疫耐受.DC的这种异质性决定了DC在肿瘤、自身免疫性疾病及移植排斥的免疫反应中起着重要的作用.     

大鼠坐骨神经缺损半个月后人工组织神经移植物修复的实验研究

为了观察人工组织神经移植物对大鼠坐骨神经非新鲜损伤的修复作用。我们在成年SD大鼠左侧股中部切除部分坐骨神经制造神经缺损模型。15d后,实验组(9只)用人工组织神经移植物修复神经缺损,自体神经修复作为阳性对照(6只),保持神经缺损为阴性对照(6只)。第二次手术后3个月,电生理学、形态学结果显示实验组的再生神经虽略差于自体对照组,但明显优于缺损对照组,腓肠肌的萎缩形态学指标变化则较接近自体对照组。实验结果表明人工组织神经移植物对已缺损15d的大鼠周围神经具有较好修复作用。     

复方枣仁水提物对小鼠学习记忆的影响研究

目的观察复方枣仁水提物对小鼠学习记忆能力的影响。方法采用跳台法观察复方枣仁水提物对记忆获得障碍小鼠、记忆巩固障碍小鼠和正常小鼠学习记忆能力的影响。结果复方枣仁水提物可明显减少记忆获得障碍小鼠在跳台中错误次数(P<0.01)和明显延长潜伏期(P<0.05),可明显减少记忆巩固障碍小鼠错误次数(P<0.05)和明显延长潜伏期(P<0.05)。结论复方枣仁可显著改善小鼠学习记忆能力。     

负压封闭引流在下肢自体植皮术中应用的临床研究

目的 探讨负压封闭引流(VSD)在下肢自体植皮术中临床应用效果.方法 收集本院2012年5月至2015年4月收治的68例需行下肢自体植皮术患者,行前瞻性研究且随机分为两组,每组34例,对照组患者给予常规换药后植皮,实验组则给予负压封闭引流植皮,比较两组患者相关临床指标、术后并发症与感染类型情况.结果 实验组患者首次植皮成功率为(88.73±3.65)％,显著性高于对照组的(78.48±3.28)％,创面修复时间为(7.12±1.07)d、住院时间为(16.17±2.06)d,术后白细胞水平为(8.25±1.34)×109/L,显著性低于对照组的[(10.28±1.44)d、(20.83±2.62)d、(12.74± 1.81)×109/L],差异具有显著统计学意义(P＜0.01);实验组患者植皮边缘坏死发生率(5.88％)明显低于对照组(23.43％),差异具有显著统计学意义(P＜ 0.01);实验组患者铜绿假单胞菌感染率(5.88％)明显低于对照组(26.47％),差异具有统计学意义(P＜0.05).结论 负压封闭引流在下肢自体植皮术中临床应用效果显著,且安全性较高,具有借鉴性.     

Spindle–slow oscillation coupling correlates with memory performance and connectivity changes in a hippocampal network after sleep

After experiences are encoded, post‐encoding reactivations during sleep have been proposed to mediate long‐term memory consolidation. Spindle–slow oscillation coupling during NREM sleep is a candidate mechanism through which a hippocampal‐cortical dialogue may strengthen a newly formed memory engram. Here, we investigated the role of fast spindle‐ and slow spindle–slow oscillation coupling in the consolidation of spatial memory in humans with a virtual watermaze task involving allocentric and egocentric learning strategies. Furthermore, we analyzed how resting‐state functional connectivity evolved across learning, consolidation, and retrieval of this task using a data‐driven approach. Our results show task‐related connectivity changes in the executive control network, the default mode network, and the hippocampal network at post‐task rest. The hippocampal network could further be divided into two subnetworks of which only one showed modulation by sleep. Decreased functional connectivity in this subnetwork was associated with higher spindle–slow oscillation coupling power, which was also related to better memory performance at test. Overall, this study contributes to a more holistic understanding of the functional resting‐state networks and the mechanisms during sleep associated to spatial memory consolidation.