分子排阻色谱法测定注射用头孢哌酮钠舒巴坦钠中的高分子聚合物

目的建立注射用头孢哌酮钠舒巴坦钠中高分子聚合物的测定方法。方法采用Sephadex G-10凝胶色谱柱(15.0mm×300mm),以pH 7.0的0.05mol.L 1磷酸盐缓冲液[0.05mol.L-1磷酸氢二钠溶液-0.05mol.L-1磷酸二氢钠溶液(61∶39)]为流动相A,以水为流动相B,流速为1.2 mL.min 1,检测波长为254 nm。结果头孢哌酮高分子聚合物与头孢哌酮药物单体能较好分离,头孢哌酮自身对照的线性范围为5.01～250.71μg.mL-1(r=0.9999);在10.13～30.24 mg.mL 1内,供试品溶液浓度与聚合物峰面积呈良好线性关系(r=0.9999);定量限为0.14μg;方法精密度良好(RSD=0.50%,n=5);样品测定重复性与重现性好(RSD=0.82%,n=5;RSD=3.4%,n=3)。结论所建方法操作简便、结果可靠,可用于注射用头孢哌酮钠舒巴坦钠中高分子聚合物的检测。     

对不同无牙颌患者选择不同基托材质的全口义齿的临床体会

目的探讨不同无牙颌患者的临床条件和个人因素选择不同基托材质的全口义修复以达到最佳效果。方法对305例无牙颌患者根据其条件分别选择甲基丙烯酸甲酯、钴铬合金、钛制基托材料进行全口义齿修复并进行临床随访。结果根据三种基托材料其优缺点结合临床选择了合适的病例进行修复均达到了与较好的临床效果,金属基托被越来越多应用于临床。结论对不同无牙颌患者应选择合适的基托材质以达到理想的修复效果,患者获得更高的满意度。     

Cranial base measurements in different anteroposterior skeletal relationships using Bjork-Jarabak analysis

ObjectivesTo assess the linear and angular cranial base measurements (Bjork polygon) in different anteroposterior (AP) skeletal relationships using Bjork-Jarabak analysis.Materials and MethodsPretreatment lateral cephalograms of 288 (146 women, 142 men, mean ages 21.24 ± 2.72 years and 22.94 ± 3.28 years, respectively) adult patients were divided into Class I, II, and III skeletal relationships according to their ANB angle. Linear and angular measurements of Bjork polygon were measured and compared among different skeletal relationships. Analysis of variance was performed to detect the differences among groups. Independent-sample t-test was used to detect differences between men and women.ResultsThe Class II skeletal relationship has a significantly larger saddle angle than Class III does (P < .05), whereas Class III has a significantly larger gonial angle than Class II does (P < .05). The articular angle and sum of Bjork polygon angles were not significantly different among groups (P > .05). Anterior (N-S) and posterior (S-Ar) cranial base lengths were similar in the different AP skeletal relationships (P > .05). The ramal height and body of the mandible length were significantly larger in Class III compared with Class I and II (P < .05). Women had a significantly larger articular angle than men did (P < .05), although men had significantly larger linear measurements of Bjork polygon than women did (P < .05).ConclusionsThe Class III skeletal relationship has a smaller saddle angle and larger mandibular length and gonial angle. Men have a larger cranial base and mandibular linear measurements and a smaller articular angle compared with women.     

啮蚀艾肯菌致鼻咽颅底感染1例报道

目的 总结1例啮蚀艾肯菌引起鼻咽颅底感染的诊疗经验,提高对该菌感染病的认识。方法 回顾性分析1例啮蚀艾肯菌致鼻咽颅底感染患者的病例资料,并结合文献进行讨论。结果 本例患者完善内镜检查、影像学检查以及实验室检查,鼻咽颅底组织2次病理报告均为慢性肉芽肿性炎,经实验室确诊为啮蚀艾肯菌感染,予清创引流、口服抗生素2个月后,患者痊愈,术后随访3个月未复发。结论 鼻咽颅底感染临床少见,临床症状、影像学检查与鼻咽癌相似。啮蚀艾肯菌为罕见致病菌,发病率低。诊治上,需结合病理学、病原学检查明确诊断,及早清创引流、选取合适的抗感染药物。     

基于淫羊藿黄酮类化合物的体内代谢阐述其抗骨质疏松药效物质基础

全面系统阐述了淫羊藿饮片中朝藿定A、朝藿定B、朝藿定C、宝藿苷I、淫羊藿苷5种主要黄酮类化合物的体内代谢过程,整理了5种黄酮类化合物的体内代谢产物与代谢途径;基于5种黄酮类化合物主要体内代谢产物,总结其共性代谢规律和共有代谢产物,并结合淫羊藿总黄酮、淫羊藿苷、宝藿苷I、淫羊藿素的抗骨质疏松药理作用及其作用机制,初步推断淫羊藿黄酮类化合物中的次糖苷和苷元是淫羊藿黄酮类化合物的主要体内代谢产物,次糖苷和苷元也可能是淫羊藿总黄酮抗骨质疏松重要药效物质基础。为淫羊藿饮片及淫羊藿黄酮类化合物抗骨质疏松药效物质基础及机制研究和抗骨质疏松有效药物研发提供了重要依据和指导。     

全口义齿固位及稳定影响因素探讨

目的探讨提高全口义齿固位及稳定的方法。方法通过对患者在试戴及应用全口义齿出现的固位稳定不良现象进行检查分析,寻找出相应的改进方法。结果全口义齿固位不良主要原因是未建立平衡,其次是制作误差及口腔解剖条件差。结论提高全口义齿固位稳定性的有效方法是确保全口义齿良好的咬合关系,提高操作精确度,以弥补口腔条件差的缺陷。     

国家中医临床研究基地（湖北）中药新药研发平台建设

从国家中医临床研究基地（湖北）的建设需要出发,创立中药新药研发平台,服务于肝病等重点病种的医院制剂和新药开发。经过5 年建设,初步形成了具有创新力的中药新药研发平台,开发了一些特色中药制剂,促进了重点病种的临床服务能力,产生了较好的经济效益和社会效益。本文就中药新药研发平台建设的成果、经验体会及展望进行探讨。     

Embryotoxicity of serum from women smoking cocaine base paste (CBP)

OBJECTIVE: Using a mouse embryo bioassay, we examined the embryotoxic properties of serum obtained from eight women who had ingested cocaine base paste (CBP) for a period of 1-3 years. STUDY DESIGN: Two-cell stage mouse embryos were cultured in the presence of serum extracted from eight CBP-smoking or control women. After 48h, embryos were evaluated to determine their developmental stage and then processed by Tarkoswki's technique to determine the proportion of embryos with micronuclei, in order to establish the number of micronuclei/embryo. RESULTS: Serum from CBP patients diminished the percentage of embryos progressing to the compacted morula stage, while increasing the proportion of embryos with micronuclei and the corresponding micronuclei/embryo ratio. CONCLUSION: These findings provide the first experimental evidence of embryotoxic compounds in the serum of CBP-smoking women. This study highlights the reproductive risk of chronically ingested CBP and demonstrates the need for further studies.     

益气解毒方阻断气虚染毒大鼠鼻咽上皮细胞癌变的研究

目的探讨益气解毒方调节NF-κB途径阻断气虚染毒大鼠鼻咽癌变的影响。方法大鼠腹腔注射二亚硝基哌嗪（DNP）和佛波醇酯（TPA）,同时进行力竭游泳,诱导大鼠诱发鼻咽癌变模型,予以益气解毒方干预,定期分批处死大鼠,取鼻咽组织做病理检测,免疫组织化学SABC法检测蛋白表达。结果在诱发鼻咽癌变进程的单纯性增生阶段,与模型组比较,益气解毒方组和维甲酸组大鼠鼻咽上皮细胞的NF-κB p65和IκBα蛋白表达活性均无统计学意义,但在异型增生阶段,益气解毒方组对鼻咽上皮细胞的NF-κB p65蛋白表达活性显著低于模型组,差异具有统计学意义（P〈0.05）,而IκBα蛋白表达活性显著高于模型组,差异具有统计学意义（P〈0.05）。结论益气解毒方能够有效阻断气虚染毒大鼠鼻咽上皮细胞癌变进程,其作用与其有效调节NF-κB途径关键基因NF-κBp65和IκBα的蛋白表达密切相关。