转基因食品的快速PCR鉴定

用十六烷基-二甲基-乙基-溴化铵(CTAB)法快速高效地从样品中提取了可供分析的基因组DNA,经PCR扩增,鉴定了含有35S启动子和NOS终止子的转基因样品.该方法的灵敏度可达0.1%,并且具有很好的稳定性.     

粘连性小瞳孔白内障超声乳化吸除及人工晶状体植入术

目的探讨粘连性小瞳孔白内障超声乳化吸除手术方法及技巧。方法对36例(42眼)粘连性小瞳孔白内障行超声乳化吸除及人工晶状体植入术,术中不分离虹膜后粘连,利用拦截劈裂法乳化晶状体核,通过4mm大小瞳孔完成手术。结果所有病例视力有不同程度提高,获得生理性圆或近圆瞳孔,主要并发症包括角膜内皮水肿,前房纤维素渗出。结论采用本方法治疗粘连性小瞳孔白内障操作相对简单,术后反应轻,并发症少。     

高效液相色谱法测定人血浆中翁丹西隆浓度

建立了翁丹西隆的高效液相色谱测定法。血浆样品用醋酸乙酯萃取，以ＺｏｒｂａｘＳｉｌｉｃａ为固定相，６０％０．０２５ｍｏｌ／Ｌ乙酸钠缓冲液（ｐＨ４．２）、４０％乙腈为流动相，紫外３１０ｎｍ处测定。采用外标法峰高定量。最低检出浓度达１ｎｇ／ｍｌ，线性范围１．０～４０．０ｎｇ／ｍｌ（ｒ＝０．９９９６），平均回收率为（９５．２２±３．５８）％（ＲＳＤ３．７６％）。用本法测定人血浆中翁丹西隆浓度具有快速、简便、专一性和灵敏度高的优点。     

Astigmatism and visual recovery after phacoemulsification and conventional extracapsular cataract extraction

In this study we wanted to investigate the post-operative astigmatism and visual acuity after phacoemulsification and conventional extracapsular cataract surgery. Patients operated between April and June 1993 (n=150) were retrospectively analyzed. The patients were examined prior to surgery and at day 1, at day 10, and in week 6 post-operatively. The difference between the post-operative log mean visual acuity in the Phaco group and in the CECCE group was significant after 1 and 10 days, however it was not significant (p=0.191) after 6 weeks. The mean astigmatism was significantly less in the Phaco group than in the CECCE group during the whole post-operative check-up period. This study suggests that Phaco results in a lower post-operative astigmatism and an earlier visual rehabilitation compared to the CECCE technique.Abbreviations Phaco Phacoemulsification - CECCE Conventional extracapsular cataract extraction     

甜茶素提取物对STZ致高血糖大鼠的降血糖作用研究

目的 :　研究甜茶素提取物对 STZ致高血糖大鼠的降血糖效果及其降糖机制。方法 :　腹腔注射 STZ制备糖尿病大鼠模型 ,取成型的大鼠随机分阳性对照组、甜茶素提取物组和降糖灵组。同时设一正常组。灌胃 3 w后 ,测血糖、果糖胺、胰岛素和 SOD等指标。结果 :　甜茶素提取物能极显著地降低 STZ致高血糖大鼠血糖 ,增强其抗氧化能力 ,同时能刺激胰岛素的分泌。结论 :　甜茶素提取物通过刺激胰岛素的分泌来降低血糖     

Comparison between postoperative astigmatism after classic extracapsular lens extraction and after phacoemulsification with implantation of a Pearce tripod or Pearce vaulted Y-loop intraocular lens

We compared the surgically induced astigmatism after standard extracapsular cataract extraction (ECCE) with the astigmatism following cataract extraction by phacoemulsification. The surgically induced corneal astigmatism was assessed on several occasions, ranging from one day to one year postoperatively. After phacoemulsification, this astigmatism was considerably slighter than after ECCE on day 1 postoperatively and after two and six weeks. However, one year postoperatively, this difference was less clear. We then measured slight against-the-rule astigmatism for both surgical techniques.     

白内障扶贫复明手术探讨

目的：探讨山区白内障扶贫复明手术。方法：采用改良现代白内障囊外摘出术或小切口非超声乳化白内障摘除术，对2675只眼白内障施行复明手术。结果：术后第一天矫正视力0．3者57只眼(2．13％)，0．5～0．8者1829只眼(68．37％)，≥0．9者509只眼(19．03％)。角膜水肿71只眼(2.65％)，后囊膜破裂致玻璃体脱出87只眼(3．25％)，全部均可同时植入人工晶体。结论：采用改良的白内障摘除手术，做到开源节流，既能使更多的贫困患者接受复明手术，又保证了手术质量。     

离子对提取比色法测定复方制剂中氨基比林的含量

复方制剂中氨基比林与溴甲酚绿形成稳定的离于对，用氯仿萃取后在４１６．８ｎｍ处测定吸收度．回收率为９９．８０％，相对标准偏差为０．９２％（ｎ＝５）。方法简便快速，结果准确可靠。     

茶叶多糖的研究进展

茶叶多糖是一种复合杂多糖，具有许多生物活性。作者系统地介绍了茶叶多糖的提取、分离、纯化、纯度鉴定及相对分子质量测定的方法，以及其含量和组成、理化性质、化学结构及空间构象、生物活性等方面的研究进展，并对上述方法及研究进展进行了分析和评述。     

芪丹煎剂有效成分提取方法优选研究

目的:研究芪丹煎剂有效成分超声法提取的最适条件.方法:利用正交试验设计,对水量、提取时间和提取次数进行分析,找出最适条件.按最适条件重复3次,考察重复性.结果:水量为14倍、提取时间为30min,提取次数为3次时有效成分的总含量最大,3次平行试验有效成分总含量RSD为0.86%.结论:超声法提取的最佳条件为:水量14倍、提取时间30min,提取次数3次.本方法重复性较好.