茶树油的提取工艺优化试验研究

目的:以茶树油提取率为考察指标,研究茶树油的提取工艺.方法:采用正交试验进行优选,气相色谱法测定4-羟基萜烯含量.结果:影响茶树油提取率的主要因素是提取时间,对茶树油的提取有显著影响;其次是加水量,对茶树油的提取有较大影响;浸泡时间的影响最小.各因素对4-羟基萜烯含量无影响.结论:最佳的提取工艺为浸泡时间2 h,提取时间6 h,加水800 ml.     

茶属植物及茶多酚类成分对抗原诱导RBL-2H3细胞过敏反应的抑制作用

目的:研究茶属植物及茶多酚类和嘌呤碱类成分对抗原刺激RBL-2H3细胞脱颗粒及肿瘤坏死因子α(TNF-α)和白细胞介素4(IL-4)释放的抑制作用。方法:测定β-hexosaminidase的释放以评价脱颗粒反应的指标,酶联免疫吸附法测定TNF-α和IL-4的释放,评价茶中主要成分的抗过敏活性。结果:茶属植物均有抑制RBL-2H3细胞脱颗粒作用,其中花类表现出较叶类更强的抗过敏活性;EGCG对抗原刺激的RBL-2H3细胞即刻相(IC50:234μmol·L-1)和延缓相反应(IL4,IC50:158μmol·L-1),及钙离子载体A23187诱导的脱颗粒反应均有抑制作用(IC50:126μmol·L-1)。结论:茶属植物和EGCG具有明显的抗过敏作用。     

消痹灵袋泡剂治疗强直性脊柱炎的临床研究

以消痹灵袋泡剂为治疗组,以消痹灵水煎剂,湿热痹冲剂分别为对照甲,乙组,共治疗强直性脊柱炎８０例。治疗组临床治愈率为２１．４３％,总有效率为９５％。与对照乙组相比具有显著差异,与对照甲组相比无显著差异。说明消痹灵袋光剂疗效明显优于湿热痹冲剂,与消痹灵水煎剂疗效相当。     

Green tea and bone metabolism

Osteoporosis is a major health problem in both elderly women and men. Epidemiological evidence has shown an association between tea consumption and the prevention of age-related bone loss in elderly women and men. Ingestion of green tea and green tea bioactive compounds may be beneficial in mitigating bone loss of this population and decreasing their risk of osteoporotic fractures. This review describes the effect of green tea or its bioactive components on bone health, with an emphasis on (i) the prevalence and etiology of osteoporosis; (ii) the role of oxidative stress and antioxidants in osteoporosis; (iii) green tea composition and bioavailability; (iv) the effects of green tea and its active components on osteogenesis, osteoblastogenesis, and osteoclastogenesis from human epidemiological, animal, as well as cell culture studies; (v) possible mechanisms explaining the osteoprotective effects of green tea bioactive compounds; (vi) other bioactive components in tea that benefit bone health; and (vii) a summary and future direction of green tea and bone health research and the translational aspects. In general, tea and its bioactive components might decrease the risk of fracture by improving bone mineral density and supporting osteoblastic activities while suppressing osteoclastic activities.     

茶多酚阴道泡腾片的制备与质量控制

目的制备茶多酚阴道泡腾片并建立其质量控制方法。方法采用正交设计试验对酸碱系统的比例进行筛选,制备茶多酚阴道泡腾片;建立发泡量、酸碱度、崩解时限等质量控制标准以考察其稳定性,并对样品中的茶多酚含量进行测定。结果该制剂为棕灰色异形片,发泡量、酸碱度、崩解时限等符合阴道泡腾片的要求,质量稳定;茶多酚检测浓度在0.02~0.08 mg mL-1范围内呈良好的线性关系(r=0.999 1),平均回收率为100.1%,RSD=0.32%(n=6),精密度良好。结论处方设计合理,质量控制方法准确、可靠。     

再论茶文化的起源、发展与功能定位

茶文化在唐代有迅猛的发展,其普及与当时的经济、政治、文化有密切的关系。也由于其含有咖啡因类成分,可以调节精神,而其他别样茶中不含此类成分,茶的普及推广,形成了一枝独秀的局面。内容进一步阐述茶文化的形成过程,作为"茶"用的植物自古以来就呈现多样性,如同维持生命的"食物"用来治疗疾病的"药品"一样,用来预防疾病的"茶"也不是单单一个物品。     

毓麟茶质量控制方法研究

目的建立毓麟茶的质量控制方法。方法采用薄层色谱法对毓麟茶中黄芪、蛇床子、五味子、枸杞子进行定性鉴别,采用高效液相色谱法测定君药五味子有效成分五味子醇甲的含量。色谱条件：Welch Materials C18色谱柱(250 mm×4.6 mm,5μm),甲醇-水(70∶30)为流动相,检测波长250 nm。结果薄层色谱鉴别方法简便,专属性强。五味子醇甲0.222～0.2220μg范围内呈良好的线性关系,r＝0.9999,平均加样回收率为98.96%,RSD＝0.74%。结论所建立的方法可用于该制剂的质量控制。     

茶叶中铝的来源及溶解性研究

本文采用微波消解法和沸水浸泡法处理茶叶、石墨炉原子吸收光谱法测定１６种茶叶中总铝和水溶性铝含量。均值分别为６０１．６ｍｇ／ｋｇ和１４８．３ｍｇ／ｋｇ。茶叶中水溶性铝占总铝含量的２４．７％。对广东省五个茶场的现场采样调查表明茶叶中的铝主要来源于茶树根系对铝的特异吸收。老叶片铝含量远高于嫩叶片是造成茶叶铝含量波动较大的主要原因     

香风茶的传统应用与研究现状△

综述文献资料,对我国别样茶之一的香风茶进行总结和探讨。香风茶为蜡梅科蜡梅属植物山蜡梅Chimonanthus nitens Oliv、柳叶蜡梅C．salicifolius S．Y．Hu和浙江蜡梅C．zhejiangensis M．C．Liu的嫩叶经加工而成,具有祛风解表,理气化痰,醒脾化浊等功效。香风茶在我国民间应用广泛,历史悠久。现代研究表明,其主要化学成分为挥发油、黄酮和生物碱类等,药理活性主要为抗菌抗病毒、降压、降脂减肥、抗氧化和抗肿瘤等作用。香风茶是一种具有广阔开发前景的茶饮,值得继续深入系统研究。     

In vitro genotoxicity of Melaleuca alternifolia essential oil in human lymphocytes

Ethnopharmacological relevance

The volatile essential oil derived from the plant Melaleuca alternifolia, also called tea tree oil (TTO), is largely employed for its antimicrobial properties against several human pathogens. It is used in many topical formulations to treat cutaneous infections.

Aim of the study

Since very few studies have been done on the safety and toxicity of the crude Melaleuca alternifolia essential oil, current investigation evaluates the possible genotoxic effects of TTO in human lymphocyte cultures.

Material and methods

The composition of current TTO sample was determined by GC/MS and NMR. The level of cytotoxicity in TTO treated cultures was determined by decrease of mitotic index when compared to that in negative control. The genotoxic potential of TTO was assessed by the in vitro mammalian cell micronucleus and the chromosome aberrations (CA) tests.

Results

Twenty-seven compounds were identified, accounting for 98.9% of the constituents. Terpinen-4-ol (42.8%), γ-terpinene (20.4%), p-cymene (9.6%), α-terpinene (7.9%), 1,8-cineole (3%), α-terpineol (2.8%) and α-pinene (2.4%) were the major compounds of the oil sample. None of the tested TTO concentrations (95 μg/ml, 182 μg/ml and 365 μg/ml) caused a significant increase in the observed frequencies of micronuclei when compared to those in the untreated cultures (negative control). Additionally, no significant differences regarding the frequencies of CA were observed among the tested TTO concentrations and the negative control.

Conclusions

Results demonstrate that TTO, in the tested concentrations, is not genotoxic in in vitro mammalian cells.