铜锌超氧化物歧化酶(Cu,Zn-SOD)的硅胶吸附分离法

从牛血或猪血的红细胞中提取铜锌SOD的关键步骤是用二乙氨基乙基纤维素将粗SOD进行纯化。我们以水玻璃为主要原料合成了成本极低的硅胶吸附剂,SOD回收率为90％。制品只有一条凝胶电泳带,效果很好,完全可以代替二乙氨基乙基纤维素用于SOD的纯化。     

肠溶性药用辅料HPMCP的合成研究

研究了肠溶性药用辅料 HPMCP 的合成工艺,投料比以50 cps HPMC:醋酸:醋酸钠:邻苯二甲酸酐为100:400:80:120为宜,于85℃反应3.5h,反应后应选择适当的沉淀剂(包括用量)、沉淀方式和搅拌速率。所得产物符合美国药典要求,主要理化性能与国外同类产品类似。     

α—FeOOH脱水过程后期的封孔机制

采用Ｇｅｒｍａｎ和Ｍｕｎｉｒ提出的烧结动力学，对文题进行了研究。结果表明，在α－ＦｅＯＯＨ分解成ａ－Ｆｅ２Ｏ３的过程中，随着温度升高，产生的孔洞通过颗粒内部的晶核表面扩散的烧结机制逐渐消失。对于包敷ＳｉＯ２的样品，相应的烧结活化能增加。当焙烧温度太高，会出现颗粒之间彼此烧结，并且未包敷的样品更易烧结。     

抗菌超滑导尿管的制备与抑菌性能研究

为了进一步降低导尿管留置时的高感染率 ,我们进行了具有抗菌性的超滑导尿管的研究。本文分别试制了两种抗菌成分的超滑导尿管 ,并对金黄色葡萄球菌、大肠杆菌尿路感染常见菌进行了抑菌试验、稳定性试验及临床验证。试验结果表明 :含抗菌成分的超滑导尿管对金黄色葡萄球菌和大肠杆菌均有一定的抑菌作用 ,但稳定性有较大差异 ,其中一种在加速试验后抑菌性消失 ,而另外一种仍保持较好的抑菌性能 ;临床观察以普通导尿管做对照 ,使用 35例 ,留置期为 7～ 30天 ,无一例出现感染症状 ,具有较明显的抗菌作用。     

溶胶—凝胶法制备TiO2—CeO2薄膜

采用溶胶－凝胶法制备了鲜黄色的高反射ＴｉＯ＿２－ＣｅＯ＿２薄膜。研究了环境湿度和醋酸添加剂对薄膜形成的影响。研究表明：醋酸加入能显著提高薄膜质量，得到均匀透明的高质量薄膜；涂膜环境温湿度对薄膜质量有决定性作用。     

生物活性梯度HA涂层人工关节的生物力学研究

[目的]探讨生物活性梯度羟基磷灰石(HA)涂层人工关节的生物力学性能。[方法]在人工股骨头置换组,将10只健康成年杂交犬随机分成2组,行右侧人工股骨头置换,分别植入钛合金人工股骨柄(Ti关节组)、HA涂层钛合金人工股骨柄(HA关节组);在圆柱体植入组,选4只健康成年杂交犬,麻醉后在股骨内外髁关节面垂直植入1个种植体,左腿植入钛合金棒(Ti圆柱体组),右腿植入羟基磷灰石涂层钛合金棒(HA圆柱体组),术后12周处死动物取材,进行生物力学测试和界面组织学观察。[结果]生物力学顶出试验显示:Ti关节组、HA涂层关节组的抗剪切强度分别为(2.58±1.56)Mpa和(7.57±1.03)Mpa,HA涂层关节组的抗剪切强度明显高于Ti关节组(P<0.01)。Ti圆柱体组、HA涂层圆柱体组的抗剪切强度分别为(2.36±1.04)Mpa和(21.65±1.48)Mpa。HA涂层圆柱体组的抗剪切强度明显高于Ti圆柱体组(P<0.01)。界面组织学观察显示:HA关节组和HA圆柱体组界面骨结合良好,骨结合率高;而Ti关节组和Ti圆柱体组界面大部分区域钛金属表面与周围的骨小梁之间有纤维组织分隔,界面骨结合率低。[结论]生物活性梯度HA涂层人工关节有良好的生物力学性能,界面较高的骨结合率是HA涂层植入体获得良好的生物力学性能的基础。     

Anticoagulant-free Genius haemodialysis using low molecular weight heparin-coated circuits.

BACKGROUND: Regional citrate anticoagulation or saline flushes are often used in haemodialysis patients at high risk of bleeding. In an alternative approach we evaluated the effects of covalent circuit coating with low molecular weight heparin (LMWH) for intermittent haemodialysis. METHODS: In vitro, we compared the thrombogenicity of an uncoated polyvinylchloride (PVC) tubing set with LMWH-coated tubing (AOThel) and a reference tubing with end-point attached heparin coating (Carmeda Bioactive surface) under dynamic blood contact. In vivo, five chronic haemodialysis patients were studied using the Genius dialysis system and F60S filters. Each patient underwent three dialysis sessions separated by a standard haemodialysis each: (1) standard dialysis (uncoated circuit and regular dalteparin dosage), (2) dialysis with LMWH-coated circuit and regular dalteparin dosage and (3) dialysis with a completely LMWH-coated circuit without anticoagulant use. RESULTS: In vitro, both coated tubings showed significantly reduced thrombin-antithrombin (TAT) complex levels compared with PVC. The reference coating (Carmeda) released substantial antifactor Xa (antiXa) activity into the plasma. The LMWH coating (AOThel) released low antiXa activity only during the initial rinsing. In vivo, all dialysis sessions were well tolerated and completed without major clotting. Antithrombin levels and platelet counts were similar in all groups. P-selectin and D-dimer levels increased similarly in all groups. TAT levels were comparable in all groups during the first 3 h and significantly increased in the anticoagulant-free group after the fourth hour. CONCLUSIONS: LMWH surface coating reduces thrombogenicity in vitro without releasing significant amounts of heparin from the surface. In vivo, anticoagulant-free haemodialysis using a completely LMWH-coated circuit is feasible and safe in stable chronic dialysis patients with normal coagulation.     

Different HPMC viscosity grades as coating agents for an oral time and/or site-controlled delivery system: a study on process parameters and in vitro performances

Currently, delayed/pulsatile release and colon delivery represent topics of remarkable interest. The present paper deals with the study and development of an oral dosage form devised to release drugs following a programmed time period after administration or, when opportune design modifications are introduced, to target the colon. The system is composed of a drug-containing core and a hydrophilic swellable polymeric coating capable of delaying drug release through slow interaction with aqueous fluids. An optional external gastroresistant film is applied to overcome gastric emptying variability, thus allowing colon delivery to be pursued according to the time-dependent approach. The aim of this work was to evaluate different hydroxypropyl methylcellulose (HPMC) viscosity grades as possible materials for the attainment of the system retarding hydrophilic layer. Both the relevant suitability for application onto tablet cores by aqueous spray-coating in fluid bed and capability of delaying drug release for a programmable period were explored and compared. Methocel E50 was found to afford the best balance among different important items, i.e. process time, retarding ability, dimensions of the coated units and possibility of finely tuning the delay duration. Further results pointed out the robustness of Methocel E50-based systems, which have shown to be practically unaffected by the concentration of the employed coating solution and the pH of the release medium, as well as only poorly influenced by ionic strength, at least with regard to values encompassed in the physiological range for gastrointestinal fluids.     

现代微丸制备与包衣装置的类型与应用

介绍了现代各种微丸制备和包衣装置的类型。阐明锅包法、流化床、多孔离心、振动喷嘴以及喷雾包衣装置的工作原理。并根据对不同种类微丸制备的工艺研究经验，对上述商用设备的特性作了比较与评价。     

云母钛珠光颜料的液相合成技术 Ⅰ．云母钛强制水解包膜工艺及机理

在文题研究中，对反应体系的ｐＨ值、反应物浓度、反应温度对合成颜料的色相和光泽等外观表现及微观结构的影响规律作了一定的工作。结果表明，较优的工艺条件：ｐＨ为０．５～１．２，铁盐浓度ｕ，为０．０３～０．０７，温度为７５～９０℃。通过ＸＲＤ研究表明，包覆在云母表面的水合二氧化钛膜层的粒子呈不明显的锐钛相微晶结构。制得的不同粒度、不同色相的云母钛具有较强的的珠光效果及明显的色调。