7β-羟基胆固醇-3,7-双琥珀酸单酯钠(RS034)对 Ehrlich 腹水癌细胞DNA、RNA及蛋白质合成的影响

以同位素体外掺入方法研究了7β-羟基胆固醇-3,7-双琥珀酸单酯钠(RS034)对小鼠 Ehrlich 腹水癌细胞 DNA、RNA 及蛋白质合成的影响。加药50μg/ml 和5μg/ml 后半小时,即对 DNA 合成产生显著的抑制作用,抑制率分别达到66.8%和52.9%;50μg/ml 剂量组对 RNA 合成也有显著的抑制作用;但上述两个剂量对蛋白质合成不仅无抑制作用,相反能显著地促进合成。RS034对肿瘤细胞核酸合成的抑制作用可能是其抗癌作用机制之一。     

5-HT4 receptor mediated stimulation of gastric emptying in rats

It is well documented that certain substituted benzamides, such as cisapride, and benzimidazolones, such as BIMU 8, enhance gastric emptying in rats. As these compounds possess 5-HT₃ antagonistic and 5-HT₄ agonistic properties, the precise mechanisms (5-HT₃ or 5-HT₄) underlying their gastroprokinesic effects is still unclear. In the present study, we used SC 49518 (a benzamide and selective 5-HT₄ receptor agonist) and two selective 5-HT₄ receptor antagonists (RS 23597-190 and SB 204070) to elucidate the role of 5-HT₄ receptors in gastroprokinesis. SC 49518 (1–316 g/kg; ip) produced significant and dose-dependent stimulation of gastric emptying in rats (ED₅₀ = 2.3 g/kg; ip). SC 49518 also produced dose-dependent inhibition of bradycardia induced by 2-methyl 5-HT (von Bezold-Jarisch reflex) but with a 156 fold lower potency (ID₅₀ = 0.36 mg/kg; ip). The gastroprokinetic effects of SC 49518 (3–316 g/kg; ip) were significantly antagonized by the selective 5-HT₄ receptor antagonist RS 23597-190 (0.1 mg/kg/min; iv). SB 204070 (0.003-1 mg/kg; ip), another selective 5-HT₄ receptor antagonist, produced dose-dependent inhibition of the gastroprokinesic effects of SC 49518 (10 g/kg; ip), the inhibition attaining statistical significance at the dose of 0.1 mg/kg; ip. RS 23597-190 had no effects on gastric emptying per se whereas SB 204070 significantly increased gastric emptying by itself at 1 mg/kg; ip but not at 0.1 mg/kg; ip. These findings show, for the first time, that SC 49518, a selective 5-HT₄ receptor agonist, produces potent stimulation of gastric emptying in rats via a mechanism involving activation of 5-HT₄ receptors. It is suggested that a similar mechanism may account for the gastroprokinetic effects of other non-selective benzamides and benzimidazolones.     

NPPB基因多态性与海南黎族和汉族原发性高血压发病相关性

目的探讨脑钠肽基因(NPPB)位点RS198389的多态性与海南黎族原发性高血压及汉族原发性高血压的关系。方法以2015年3月—2016年3月陵水自治区县人民医院黎、汉族住院的原发性高血压患者272例(黎族136例,汉族136例)作为实验组,同时选取黎族汉族健康人群272例(黎族136例,汉族136例)作为对照组,各组间在年龄性别等方面差异无统计学意义(P0.05)。采用等位基因特异性PCR方法,对NPPB基因RS198389突变位点进行多态性检测,并比较两组基因型频率和等位基因频率。结果黎族高血压组的NPPB基因RS198389多态性位点的TT、TC、CC基因型频率分布为50.7%、44.1%、5.2%,黎族正常对照组TT、TC、CC基因型频率分布为72.8%、23.5%、3.7%,分布差异有统计学意义(P0.05)。汉族高血压组的NPPB基因RS198389 TT、TC、CC基因型频率分布为17.6%、77.2%、5.2%,汉族健康对照组TT、TC、CC基因型频率分布为63.2%、34.6%、2.2%,分布差异有统计学意义(P0.05)。结论NPPB基因RS198389多态性位点与海南岛汉族和黎族人群原发性高血压的发病均具有相关性;汉族和黎族组间比较,NPPB基因RS198389多态性位点与黎族高血压的相关性更为明显。     

无鼻内窥镜下RS置管术治疗泪小管断裂的临床疗效

目的：探讨无鼻内窥镜下RS置管术治疗泪小管断裂的临床疗效。

方法：回顾性分析我院2017-09/2018-03收治的泪小管断裂患者52例52眼。根据入院时间顺序分为两组,RS管组32例32眼,环形泪道置管组20例20眼。对比分析两组患者的术中鼻出血、临床疗效和术后并发症情况。

结果：RS管组术中无鼻出血发生,环形泪道置管组鼻黏膜出血15眼,两组差异有统计学意义(P<0.001)。RS管组治愈26眼,好转3眼,无效3眼,治疗有效率为91%; 环形泪道置管组治愈15眼,好转3眼,无效2眼,治疗有效率为90%,两组临床疗效差异无统计学意义(P=0.877)。术后2wk～2mo,RS管组脱管2眼,有1眼在留置未满2wk时从泪小点脱出,给予再次吻合; 另有1眼在术后1～2mo内从泪点抽出,未再次手术,随访时泪道冲洗好转。环形泪道置管组脱管3眼,未再次手术,在以后的随访过程中泪道冲洗好转。两组患者术后并发症差异无统计学意义(P=0.361)。

结论：泪小管断裂需及时手术治疗,采用RS管能提高手术成功率,降低并发症的概率,并且具有操作方法简单、组织相容性好、不影响患者外观等优点。     

Protective efficacy of ursodeoxycholic acid nanoparticles in animal model of inflammatory bowel disease

Abstract

Context: Ursodeoxycholic acid (UDCA) exerts dose-dependent chemoprevention in colonic inflammation. Polycationic UDCA nanoparticles (UNPs) are envisaged for solubility enhancement and site directed drug delivery. Objective: The objective was to study the ameliorative efficacy of UNPs through localized delivery of the drug. Methods: UNPs were prepared through nanoprecipitation technique. Particle size, morphology, in vitro drug release and in vivo protective efficacy in inflammatory bowel disease (IBD) of these nanoparticles were studied. Results and Discussion: The average particle size was around 100?nm, and the average drug encapsulation was about 99%. In vitro drug release study shows optimal drug release in simulated colonic fluid. The lowering of tissue nitric oxide, malondialdehyde, myeloperoxidase and histology of the colon tissue supported the protective efficacy of the nanoparticles. Conclusion: This study presents the improved efficacy of UNPs in animal model of IBD due to complete release of drug at the desired site of action.     

医院水、电、氧气表智能采集与管理系统的设计与实现

目的：研制开发一种适用于医院内网环境下的科室水、电、氧气表采集与管理系统,并提供与＂军卫一号＂成本核算软件的接口,做到成本录入准确、非人为化。方法：终端设备采用带有RS-485远传接口的水、电、氧气表,将每个独立核算科室的所有远传仪表接入到一个或一组串口服务器中,通过自己编写的底层通讯、数据采集软件将定期采集到的所有设备数据发送并存储到一个或多个建立于＂军卫一号＂数据库平台的专用数据表当中供访问。结果：已经实际应用到医院新住院大楼并达到预期效果。结论：通过利用串口服务器将分散的设备及数据汇合到医院局域网中,这一设备接口模式具有很广泛的应用空间和发展前景。     

控释包衣膜中增塑剂含量的HPLC测定

建立了高效液相色谱法测定控释包衣膜中增塑剂含量,采用Hypersil C18柱,甲醇为流动相,检测波长为217和260nm,分别测定柠檬酸三乙酯（TEC）,柠檬酸三丁酯（TBC）,三醋酸甘油酯（TR）,邻苯二甲酸二甲酯（DMP）,邻苯二甲酸二乙酯（DEP）,邻苯二甲酸二丁酯（DBP）的含量,其中DMP,DEP和DBP在5－87ug/ml,TEC,TBC,TR在130－2300ug/ml浓度范围内与峰高呈良好的线性关系（r=0.9999),方法的日内及日间精密度,回收率均符合药物分析要求。