HGF/c-Met、VEGF和uPA在口腔鳞癌组织中的表达及意义

目的：探讨口腔鳞癌中HGF/c-Met、VEGF和uPA的表达及其相关性。方法：应用免疫组织化学SP法检测63例口腔癌、20例正常口腔黏膜的HGF/c-Met、VEGF和uPA蛋白表达。结果：①HGF/c-Met、VEGF和uPA阳性表达率：口腔鳞癌中分别为98.41%、100%、92.06%、88.89%,均显著高于正常口腔黏膜中表达率（5.00%、10.00%、20.00%、15.00%）,P〈0.05;有淋巴结转移的口腔癌组强阳性表达率分别为72.73%、77.27%、72.73%、68.82%,高于无转移组（53.66%、46.34%、46.34%、43.90%）。T3、T4期HGF/c-Met、VEGF和uPA的强阳性表达（93.10%、82.76%、72.41%、79.31%）高于T1、T2期（32.35%、35.29%、41.18%、29.41%）,P〈0.05。②口腔鳞癌中HGF/c-Met、VEGF和uPA表达呈正相关。结论：HGF/c-Met、VEGF和uPA表达与口腔癌的发生、发展相关,对口腔鳞癌的浸润、转移起促进作用且相互协同,是口腔鳞癌侵袭的重要标志物。     

DNA histochemical analysis in oral squamous cell carcinoma

BACKGROUND: Recent years have witnessed an increasing emphasis on the role of nuclear DNA and its application in experimental pathological diagnosis to predict prognosis and management of certain neoplasm. AIMS: to establish objective criteria for the degree of differentiation and histochemical quantitative of nuclear DNA of oral squamous cell carcinoma (OSCC) using microspectrophotometric analysis. MATERIAL AND METHODS: The study was conducted on histologic materials from patient with OSCC. Two histological grading systems were used; Broder's and invasive front grading system were recorded. Microspectrophotometry was applied on Feulgen-stained sections to determine the quality of tumour nuclear DNA content in two different histological grading systems of OSCC. RESULTS: Nuclear DNA content increased significantly with decreasing tissue differentiation as well with increasing tumour size. CONCLUSION: The grading system and DNA content provides more objective and accurate criteria which relate the morphologic finding to biologic activity and growth patterns of oral cancer as compared to histologic differentiation alone.     

The Role of Increased Connective Tissue Growth Factor in the Pathogenesis of Oral Submucous Fibrosis and its Malignant Transformation—An Immunohistochemical Study

Connective tissue growth factor (CTGF), a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins, is highly expressed in various organ fibrosis and several malignant tumors. Although a few studies have been conducted using CTGF in oral submucous fibrosis (OSF) and oral squamous cell carcinoma, no study has demonstrated its relation with various stages of OSF and its malignant transformation. The present study investigated the possible role of CTGF in the pathogenesis of OSF and its malignant transformation by using immunohistochemistry. Ten formalin-fixed paraffin-embedded tissue blocks, each of Stage 1 OSF, Stage 2 OSF, Stage 3 OSF, Stage 4 OSF, well- differentiated squamous cell carcinoma (WDSCC) with OSF and WDSCC without OSF were stained for CTGF by immunohistochemistry. Ten cases of healthy buccal mucosa (NOM) were included as controls. The present study demonstrated a statistically significant expression of CTGF in the epithelium and connective tissue of OSF and WDSCC with and without OSF cases against its complete absence in NOM. We observed an upregulation of CTGF expression from NOM to various stages of OSF to WDSCC with or without OSF. A gradual upregulation of the CTGF expression in various stages of OSF to WDSCC (with and without OSF) against its complete absence in NOM suggests that CTGF plays an important role in the pathogenesis of OSF and its malignant transformation.     

口腔鳞状细胞癌不同区域的P53、P21基因蛋白表达差异

目的:通过对口腔鳞癌的手术切除标本划分不同区域,研究不同区域的病理分级,以及p53、p21基因蛋白表达差异.方法:分别对表面区、中心区、深层浸润区进行病理分级(WHO),并统计观察其差异性.采用S-P免疫组织化学染色方法来检验口腔鳞癌标本的不同区域的p53、p21基因蛋白表达差异.结果:20例口腔鳞癌标本中表面区70%属病理Ⅰ级,无一例Ⅲ级,中心区则65%属病理Ⅱ级,深层浸润区70%为病理Ⅲ级,无一例Ⅰ级;两两比较,差异有显著性(P＜0.01).p53和p21基因蛋白表达亦随病变的区域出现不同阳性表达率.结论:本研究发现口腔鳞癌在其表面区域、中央区域、深层浸润区域,病变的分化程度有明显差异,即自表面至中心至浸润区分化程度越来越差.p53和p21基因蛋白的表达亦出现类似趋势,即随着病变进展阳性表达率亦随之增高.     

PTEN、p53在口腔黏膜白斑和口腔鳞癌组织中的表达及意义

目的:探讨抑癌基因PTEN、p53在口腔黏膜白斑(oralleukoplakia,OLK)和口腔鳞癌(oralsquamouscellcancer,OSCC)组织中的蛋白表达及意义。方法:应用免役组化S-P法检测10例正常口腔黏膜、25例口腔黏膜白斑(其中白斑伴上皮异常增生15例,白斑不伴上皮异常增生10例)及32例口腔鳞癌组织中抑癌基因PTEN和p53的蛋白表达情况,分析两者之间的相互作用关系及其在口腔鳞癌发生、发展过程中的作用。结果:正常口腔黏膜和白斑不伴上皮异常增生组织中PTEN蛋白全部阳性表达;白斑伴上皮异常增生组织中PTEN蛋白阳性表达率为93. 3%;OSCC组织中PTEN蛋白的阳性表达率为71. 9%,其中高、中、低分化OSCC组织中PTEN蛋白的阳性表达率分别为85. 7%、75%和33. 3%,统计学分析表明PTEN在OSCC组织中的蛋白表达与组织分化程度明显相关(P<0. 05)。正常口腔黏膜组织中p53蛋白阴性表达;白斑组织中p53蛋白的阳性表达率为48%;OSCC组织中p53蛋白的阳性表达率为56. 3%,其中高、中、低分化OSCC组织中p53蛋白的阳性表达率分别为57. 1%、58. 3%和50%,统计学分析表明p53在OSCC组织中的蛋白表达与组织分化程度无明显相关(P>0. 05)。p53阴性表达的14例OSCC组织中有5例PTEN也阴性表达。结论:OSCC组织中PTEN和p53蛋白异常表达,说明PTEN和p53基因突变或缺失     

Growth inhibition and induction of apoptosis in human oral squamous cell carcinoma Tca-8113 cell lines by Shikonin was partly through the inactivation of NF-kappaB pathway

Shikonin, a naphthoquinone pigment isolated from the Chinese herbal therapeutic, Zicao, has been shown to exhibit antioxidant and anticancer effects. In this study, its ability to induce apoptosis in cultured Tca-8113 oral cancer cells was studied. Treatment of the Tca-8113 cells with a variety of concentrations of Shikonin (10-40 microm) resulted in dose- and time-dependent sequences of events marked by apoptosis, as shown by the loss of cell viability, chromatin condensation, internucleosomal DNA fragmentation and sub-G1 phase accumulation. Furthermore, apoptosis in the Tca-8113 cells was accompanied by the activation of protease caspase-8, -9, -3 and low expression of Bcl-2 protein. Interestingly, inactivation of the NF-kappaB pathway was found in shikonin-induced apoptosis in Tca-8113 cells. These results raise the possibility that the anti-tumor effects of Shikonin in Tca-8113 cells are at least partly through the inactivation of the NF-kappaB pathway and subsequent activation of protease caspase family. Pharmacological inhibition of the NF-kappaB activity by Shikonin might be a powerful treatment option for OSCC in which activation of NF-kappaB plays a critical role in tumor growth and progression.     

High risk human papillomavirus in oral squamous carcinoma: evidence of risk factors in a Venezuelan rural population. Preliminary report

In a search for the presence of human papillomavirus (HPV) and some etiologic cofactors in oral squamous cell carcinoma (OSCC), 50 women diagnosed as OSCC were analyzed by a multiplex polymerase chain reaction (PCR) assay specific for HPV types 6, 11, 16, and 18. This study revealed that 60% (30/50) of the OSCC patients were positive for HPV-DNA sequences. This group was analyzed according to smoking, alcohol consumption, number of pregnancies, poor oral health and low social economic status. The current results indicate an increased incidence of HPV malignant types in the oral cavity in women with OSCC. Also, they support a multifactorial model of oral cancer causation.     

bcl-2与p53基因在口腔白斑及口腔鳞癌中表达的意义

目的:研究p53和bcl-2的基因产物在正常口腔粘膜(NOM)、口腔粘膜白斑(OLK)及口腔鳞癌(OSCC)中的表达及意义.方法:应用免疫组织化学S-P法,对NOM(10例)、OLK(17例)、OSCC(32例)中p53和bcl-2的表达情况进行测定.结果:p53在正常NOM中无表达,在OLK中有中度表达(阳性率29.4%),在OSCC中有较强的表达(阳性率56.25%);bcl-2在NOM中无表达,在OLK中有较强的表达(阳性率23.53%),而在OSCC中的表达(阳性率21.88%)与OLK中的表达比较差异无显著性.结论:① p53的突变和bcl-2的过度表达,通过抑制细胞凋亡促进了OLK和OSCC的形成;②在OLK的形成过程中,突变型p53和bcl-2的基因产物表达呈正相关,而在OSCC的形成过程中,二者的基因产物无明显相关性;③ bcl-2在OLK和OSCC的形成过程中,并非单纯通过抑制细胞凋亡这一机制而起作用;④突变型p53的表达可作为OLK及OSCC的基因标志物.     

Studies of the serum HER-2/neu and squamous cell carcinoma-related antigen expression in patients with oral squamous cell carcinoma

BACKGROUND: Over-expression of HER-2/neu has been associated with advanced disease, metastasis, and poor clinical outcome. Squamous cell carcinoma (SCC)-related antigen (SCCA) is used as a tumor marker in a variety of SCC. This study was performed to investigate the pre- and post-treatment values of HER-2/neu and SCCA in patients with oral SCC. MATERIALS: Patients with OSCC were enrolled between 2002 and 2004. Serum samples were obtained before treatment and at 1 month following treatment. HER-2/neu levels and SCCA levels were determined by enzyme-linked immunosorbent assay and radioimmunoassay, respectively. RESULTS: A significant correlation was found between HER-2/neu and lymph node metastasis (P = 0.0252). SCCA correlated with tumor size (P = 0.0042). Both HER-2/neu and SCCA mean levels were reduced significantly after treatment. CONCLUSION: The combination of HER-2/neu and SCCA serum levels may be a useful marker in evaluating therapeutic effects and in monitoring the recurrence rate of OSCC and survival rate of patients.     

C-erbB-2 mRNA 在口腔鳞癌组织中的表达及意义

目的 探讨C-erbB-2基因在口腔鳞癌组织中的表达及其在口腔鳞癌发生发展中的作用。方法 选择80例口腔癌根治标本按照原发灶的分期、病理分级、以及有无淋巴结转移分组，用C-erbB-2寡核苷酸探针原位杂交检测其在口腔鳞癌中的表达。结果 C-erbB-2 mRNA在口腔鳞癌中的阳性表达率与口腔癌的组织分级和临床分期有关，但与淋巴结转移无关。结论 C-erbB-2异常表达在口腔癌发生发展和淋巴结转移中起重要作用，检测C-erbB-2基因对判断口腔癌患者预后和淋巴结转移有一定的参考价值。