斑马鱼牙齿的组织结构和超微结构特征

目的：观察成年斑马鱼牙齿的组织结构和超微结构特征。方法：以成年斑马鱼（鱼龄〉3月）为研究对象,在体视显微镜下,解剖成年斑马鱼腮弓,对其腮弓和牙齿进行阿辛兰和茜素红染色观察、扫描电镜观察和石蜡切片苏木精-伊红染色观察。结果：阿辛兰和茜素红染色显示斑马鱼牙齿位于第五腮弓上,斑马鱼腮弓和牙齿为硬组织结构,具有透明的牙釉质和红染的牙本质。扫描电镜和苏木精-伊红染色显示斑马鱼牙齿的硬组织有类似人牙釉质和牙本质样结构,斑马鱼牙齿也具有牙髓腔,髓腔内也有牙髓样软组织结构,牙髓腔硬组织壁上具有丰富的牙本质小管样结构。结论：成年斑马鱼牙齿在组织结构和超微结构上与人类牙齿具有相似性。     

创伤性兔股动脉瘤、动静脉瘘模型的建立及相关研究

目的:探讨建立实验性兔股动脉瘤、动静脉瘘模型的可行性,并进行相关研究.方法:用显微外科技术在40只新西兰白兔股部建立股动脉瘤、股动静脉瘘模型,行模型的血流探测,血气分析,彩色多谱勒血流成像(CDFI),CTA,MRA,DSA检测,并行组织学观察.结果:20只新西兰白兔中成功制作动脉瘤16个,成功率80%,20只新西兰白兔中全部成功制作股动静脉瘘,并经相关检测手段证实.结论:显微外科技术建立兔股动脉瘤,动静脉瘘模型切实可行,检测方法上表现各具特点.     

慢性肾盂肾炎大鼠模型的建立

目的创建一种慢性肾盂肾炎(CPN)大鼠模型.方法分别用108/ml(A组)、106/ml(B组)浓度致病性大肠杆菌(E.coli O111B4)株膀胱内注射,并短暂结扎左侧输尿管,观察7、15、30、60、90d肾脏形态学变化,肾组织及膀胱尿细菌培养,以及30、60、90d尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG)/尿肌酐、血肌酐(Scr)、尿素氮(BUN)、24h尿蛋白定量的变化.结果随着感染性炎症的修复,左肾外观有皮质瘢痕及相关肾乳头收缩和肾盏的扩张,镜下慢性肾间质性肾炎改变和纤维化,以A组显著;A、B两组NAG/尿肌酐在第90日明显升高;细胞培养阳性结果主要在左肾和尿;其余项目与C组无差异.结论短暂结扎单侧输尿管和膀胱内接种一定剂量E.coli O111B4能够制备典型的CPN大鼠模型,以108/ml浓度为适宜.     

肝癌危险因素分析及其神经网络研究

目的 探讨福建省肝癌发病的主要危险因素;建立肝癌危险因素神经网络预测模型.方法 采用病例-对照研究.用问卷进行调查.用logistic回归分析筛选出主要危险因素.建立肝癌危险因素神经网络模型.结果 感染乙型肝炎、经常食用腌制食品、霉变食品处理后食用等6项.是肝癌的危险因素;吃饭时间≥10 min为保护因素;神经网络预测...     

健康状态辨识模型算法的探讨

健康状态辨识模型算法是对中医辨证思维的数学建模与实现。模型算法在求解表征参数对状态要素贡献度的基础上,还必须遵循中医理论体系和思维规律,充分考虑表征参数的表达和分类的差异、隐性参数与证候真假、状态要素的兼杂与缓急等问题。文章在总结分析了"中医专家系统"和原有辨证模型利弊的基础上,提出了中医健康状态辨识模型算法研究的基本框架。本领域的探索将有助于推动中医健康理论创新,同时也为健康状态辨识工具的研发提供方法学平台。     

Optimization of nonlinear dose- and concentration-response models utilizing evolutionary computation

An essential part of toxicity and chemical screening is assessing the concentrated related effects of a test article. Most often this concentration-response is a nonlinear, necessitating sophisticated regression methodologies. The parameters derived from curve fitting are essential in determining a test article's potency (EC(50)) and efficacy (E(max)) and variations in model fit may lead to different conclusions about an article's performance and safety. Previous approaches have leveraged advanced statistical and mathematical techniques to implement nonlinear least squares (NLS) for obtaining the parameters defining such a curve. These approaches, while mathematically rigorous, suffer from initial value sensitivity, computational intensity, and rely on complex and intricate computational and numerical techniques. However if there is a known mathematical model that can reliably predict the data, then nonlinear regression may be equally viewed as parameter optimization. In this context, one may utilize proven techniques from machine learning, such as evolutionary algorithms, which are robust, powerful, and require far less computational framework to optimize the defining parameters. In the current study we present a new method that uses such techniques, Evolutionary Algorithm Dose Response Modeling (EADRM), and demonstrate its effectiveness compared to more conventional methods on both real and simulated data.     

膝关节三维有限元模型的建立

目的建立完整的膝关节三维有限元模型。方法获取正常膝关节SCT扫描图像数据,使用3D-Doctor、Geomagic Studio、ABAQOUS等软件建立膝关节三维有限元模型。结果建立的膝关节三维有限元模型具有良好的生物形态,外形与实体标本一致性高。结论采用CT扫描资料建立三维有限元模型是切实可行的,简便、高效,特别适用于骨科领域三维有限元模型的建立。     

Comparison of the Child-Turcotte-Pugh classification and the model for end-stage liver disease score as predictors of the severity of the systemic inflammatory response in patients undergoing living-donor liver transplantation

The aim of this study was to evaluate and compare the Child-Turcotte-Pugh (CTP) classification system and the model for end-stage liver disease (MELD) score in predicting the severity of the systemic inflammatory response in living-donor liver transplantation patients. Recipients of liver graft were allocated to a recipient group (n = 39) and healthy donors to a donor group (n = 42). The association between the CTP classification, the MELD scores and perioperative cytokine concentrations in the recipient group was evaluated. The pro-inflammatory cytokines measured included interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α; the anti-inflammatory cytokines measured included IL-10 and IL-4. Cytokine concentrations were quantified using sandwich enzyme-linked immunoassays. The IL-6, TNF-α, and IL-10 concentrations in the recipient group were significantly higher than those in healthy donor group patients. All preoperative cytokine levels, except IL-6, increased in relation to the severity of liver disease, as measured by the CTP classification. Additionally, all cytokine levels, except IL-6, were significantly correlated preoperatively with MELD scores. However, the correlations diminished during the intraoperative period. The CTP classification and the MELD score are equally reliable in predicting the severity of the systemic inflammatory response, but only during the preoperative period.     

芍甘多苷对四氯化碳亚急性肝损伤大鼠肝脏基因表达谱的影响

目的 研究芍药甘草汤提取物芍甘多苷对四氯化碳(CC14)亚急性肝损伤大鼠血清转氨酶、肝脏病理及肝脏基因表达谱的影响.方法 60只SD大鼠完全随机分6组(n=10),即正常组、模型组、阳性对照组(联苯双酯100 mg/kg)和芍甘多苷低、中、高3个剂量组(88、264、528 mg/kg).采用CCl4诱发大鼠亚急性肝损伤,然后给予不同剂量的芍甘多苷灌胃,观察药物对血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、肝脏组织病理的影响;并运用基因表达谱芯片技术,研究其对肝脏基因表达谱的影响.结果 与模型组比较,芍甘多苷高、中剂量灌胃给药均能显著降低CCl4亚急性肝损伤大鼠血清ALT[ (218.37± 114.59,234.59± 109.33) U/L比(389.12±142.57) U/L,P＜0.05];且高剂量芍甘多苷可以显著降低肝脏组织学评分(2.42±0.52比3.92±0.84,P＜0.05).芍甘多苷能上调和(或)下调与肝损伤相关的多种基因的表达.结论 芍甘多苷有明显的肝脏保护作用,其机制与调节与肝损伤相关的多种基因的表达有关,是多靶点作用药物.