健康志愿者龈沟液中头孢哌酮注射液药物浓度的检测

目的：检测单次静滴头孢哌酮注射液的健康志愿者龈沟液中的药物浓度。方法：对8名健康志愿者单次静滴头孢哌酮舒巴坦注射液（2：1）,采用高效液相色谱法测定龈沟液中头孢哌酮的药物浓度。结果：龈沟液样品中头孢哌酮色谱分离完全,检测灵敏度高,最低检测浓度可达到10ng／ml,回收率及线性关系良好,该药吸收迅速,给药中15min即可在龈沟液中检测出。结论：高效液相色谱法可以简便,准确迅速的检测出龈沟液中的头孢哌酮含量。     

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目的探讨计算机辅助设计与制作(CAD/CAM)纯钛烤瓷冠修复对患牙牙周组织的影响。方法选择2008年1月至12月到石家庄市第一医院口腔科就诊的前磨牙有大面积牙体缺损患者20例(30颗牙),采用金属桩核加CAD/CAM纯钛烤瓷冠修复。在牙体预备前及修复体戴入后8个月,分别对龈沟液(GCF)量及GCF中碱性磷酸酶(ALP)活性及肿瘤坏死因子-α(TNF-α)质量浓度进行检测,并对检测结果进行分析比较。结果修复体边缘的深度及密合度均符合临床要求,修复后GCF量、ALP活性及TNF-α质量浓度与修复前比较差异无统计学意义(P>0.05)。结论短期内,临床合格的纯钛烤瓷冠对牙周组织未产生明显不利影响。     

Calprotectin and cross-linked N-terminal telopeptides in peri-implant and gingival crevicular fluid

In a previous study on guided bone augmentation, a new collagen membrane was compared with an e-PTFE one on 28 partially edentulous patients who received 50 dental implants at stage 2 surgery. After implant integration and subsequent loading, we were able to recruit 22 patients with 22 implants and their contra-lateral corresponding teeth for longitudinal observation. Clinical parameters probing depth (PD), bleeding on probing (BoP), plaque index (PI), assessment of gingival crevicular fluid (GCF) and peri-implant crevicular fluid (PCF) volumes and periapical radiographs were performed at 2- and 3-year control appointments. Calprotectin (MRP 8/14) and cross-linked N-terminal telopeptide (NTx) levels in both crevicular fluids were determined by ELISA. PD was significantly reduced from years 2 to 3 appointments at implant sites as at teeth sites. At the 3-year appointment in both compartments, fluid volumes were significantly increased, which corresponded well with ascending NTx levels. The total amount of calprotectin decreased non-significantly in both GCF and PCF samples. Periapical radiographs revealed stable bone conditions around implants without significant changes from years 2 to 3 examinations. Clinical peri-implant parameters were considered as stable as the periodontal parameters of their corresponding teeth. A parallel increase in NTx levels in both GCF and PCF at 3-year appointment is not clearly understood; it may reflect an upregulation in the overall bone turnover rate.     

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目的探讨镍铬合金烤瓷冠修复对牙周组织的影响。方法选择2005年9月至2006年4月在南京军区福州总医院476临床部口腔科采用镍铬合金烤瓷冠进行上颌切牙修复的19例患者,共23颗镍铬合金烤瓷冠修复的上颌切牙,以患牙对侧的同名健康牙为对照牙,在镍铬合金烤瓷冠戴入6~9个月后复诊,进行牙周临床检查,分别采集患牙及对照牙的龈沟液进行定量,并对龈沟液中的C-反应蛋白(CRP)和肿瘤坏死因子-α(TNF-α)进行检测。结果符合临床合格要求的镍铬合金烤瓷冠修复后,患牙的菌斑指数没有明显改变,但龈沟液量、探诊深度、龈沟内出血指数、龈沟液内CRP、TNF-α增高。结论镍铬合金烤瓷冠对患牙的牙周组织有一定的不良影响。     

慢性牙周炎患者基础治疗前后龈沟液中肝细胞生长因子水平的变化

目的 观察HGF在慢性牙周炎患者基础治疗前后的变化.方法 用滤纸条的袋内取样法取15例慢性牙周炎患者30个牙位治疗前后的GCF样本,采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)测定其中的HGF水平.结果 治疗后慢性牙周炎患者的HGF水平较治疗前显著下降(P＜0.01).结论 HGF水平反映牙周组织的炎症破坏程度,可作为评价疗效的客观指标,在判断牙周组织的活动性破坏中有意义.     

复方茶多酚含漱液对牙周炎患者龈下菌群及龈沟液内毒素水平的影响

目的:观察牙周洁刮治术和局部应用口灵(复方茶多酚含漱液)对成人牙周炎患牙龈下菌群及龈沟液中内毒素水平的影响.方法:选择78例成人牙周炎共156颗患牙,随机分为实验组、阴性对照组和阳性对照组.实验组给予牙周洁刮治术和口腔局部应用复方茶多酚含漱液漱口,阴性对照组给予牙周洁刮治术和蒸镏水漱口,阳性对照组给予牙周洁刮治术和0.2%洗必泰液漱口.三组分别于治疗前后检测其龈沟液中内毒素含量,并对龈下细菌进行分离培养.结果:三组治疗后龈沟液中内毒素水平及龈下细菌的数量较治疗前明显降低(P＜0.05).实验组治疗后龈沟液中内毒素水平明显低于阴性对照组(P＜0.05);龈下细菌的数量及组成发生明显改变,龈下细菌总数及革兰氏阴性杆菌较阴性对照组明显减少(P＜0.05),革兰氏阳性球菌增加(P＜0.05).结论:牙周洁刮治术合并复方茶多酚含漱液局部应用对患牙龈下菌群及龈沟液中内毒素水平有明显影响.     

三种冠边缘设计对龈沟液中内毒素含量的影响

目的:通过测定冠修复前后龈沟液内毒素含量的变化,评价3种冠边缘设计对牙龈状况与龈沟液中内毒素含量的影响。方法:将要求烤瓷熔附金属全冠修复的就诊患者,随机分为A:肩台组、B:浅凹型肩台组、C:刃状边缘组,每组各10例。分别将修复前、修复后1个月、6个月的龈沟液样本内毒素量、龈沟液量及牙龈指数进行比较,统计学处理。结果:3组患牙冠修复后1个月各项指标明显上升。冠修复后6个月A、B两组各项指标下降至接近牙体预备前水平;C组内毒素水平无下降,牙龈指数、龈沟液量与修复后1月相比有所下降但无统计学意义。结论:只要按要求制作,A、B组龈边缘形式的修复均具有良好的效果,修复效果明显优于C组。     

糖尿病患者义齿修复后龈沟液中白介素-1变化的实验研究

目的：探讨糖尿病患者戴用可摘局部义齿后龈沟液中白介素-1（IL-1）的变化,从而为临床上糖尿病患者义齿修复方式的选择提供依据。方法：对牙列缺损的糖尿病患者进行钴铬合金铸造支架式可摘局部义齿修复,在修复后六个月时分别采取基牙和非基牙区的龈沟液,用酶联免疫吸附法测定分析IL-1的表达水平。结果：可摘局部义齿修复后,糖尿病患者近缺隙侧基牙区龈沟液中IL-1的表达水平明显升高,而非基牙区则无明显改变。结论：糖尿病患者可摘局部义齿修复对基牙龈沟液中IL-1的表达水平有影响,从而为临床义齿修复提供了一定的理论依据。     

可摘局部义齿修复后龈沟液中白介素-1变化的实验研究

目的：探讨可摘局部义齿对龈沟液中白介素-1（IL-1）表达水平的影响,为临床可摘局部义齿设计和制作提供依据。方法：对牙列缺损患者进行铸造支架式可摘局部义齿修复,修复后6个月,分别采取近缺隙侧基牙区和非基牙区龈沟液,用酶联免疫吸附法测定分析龈沟液中IL-1的表达水平。结果：可摘局部义齿修复后,近缺隙侧基牙区龈沟液中IL-1的表达水平升高,非基牙区无明显改变。结论：对于可摘局部义齿修复者,近缺隙侧基牙龈沟液中IL-1的表达水平升高,且明显高于非基牙区龈沟液IL-1水平。     

Activity and inhibition of elastase in GCF

Abstract. The aim of this study was to investigate the presence of free elastase in GCF. Samples were taken from inflamed sites in 12 subjects with gingivitis alone and from inflamed sites with and without tissue destruction in 19 patients having periodontitis. Elastase activity was measured with a low molecular weight substrate. To distinguish between free elastase and elastase bound to α-2-macroglobulin (A2MG), an excess of α-1-antitrypsin (AlAT) was added to the samples. The activity that could be inhibited by AlAT was considered as free elastase, and the uninhibited activity as derived from the elastase-A2MG complex. The elastase- A1AT complex was measured with an ELISA. Free elastase was found in almost all samples, but both the total amount and the proportion of free elastase were higher in samples from sites showing destruction. The elastase-A2MG complex was also increased in sites with tissue destruction, while there was no significant difference in the amount of AlAT complex between the 3 categories of sites. In conclusion , our study clearly reveals free elastase in GCF. The elevated levels of free elastase in sites showing tissue destruction seem to be due to a combination of increased release of elastase and an inactivation of AlAT.