侵袭性牙周炎治疗前后龈沟液CRP、sICAM-1含量测定

目的：测定侵袭性牙周炎龈沟液中C反应蛋白、可溶性细胞间黏附分子1的含量及牙周非手术治疗对其水平的影响,方法：16例侵袭性牙周炎病例,进行非手术治疗,收集治疗前后龈沟液,用酶联免疫吸附法（ELISA法）测定C反应蛋白（CRP）、可溶性细胞间黏附分子1（sICAM—1）水平,并和正常对照组比较。结果：侵袭性牙周炎患者龈沟液中CRP、sICAM-1水平增高,和对照组比较,p〈0．05;牙周非手术治疗后,牙周临床指标明显改善,龈沟液中CRP、sICAM-1水平下降,且接近牙周正常水平。结论：侵袭性牙周炎龈沟液CRP、sICAM-1的水平升高,牙周非手术治疗能有效降低CRP、siCAM-1在龈沟液中的表达。     

Smoking and GCF levels of IL-1beta and IL-1ra in periodontal disease

AIMS: GCF levels of the cytokine IL-1beta and its receptor antagonist IL-1ra were analyzed with respect to smoking in patients with moderate to severe periodontal disease. The study population included 22 smokers and 18 non-smokers in the age range 32-86 years. Concomitantly, the GCF levels of IgA, IgG, albumin and total protein were analyzed. METHOD: Samples of GCF were obtained from 2 diseased sites in each patient by means of an aspiration method. IL-1beta, IL-1ra, IgA and IgG were determined with immunoelectrophoresis. Total protein was determined by the BCA method. RESULTS: The clinical characteristics in terms of probing depth and frequency of diseased sites and supragingival plaque did not differ between smokers and non-smokers. Gingival bleeding, however, was significantly depressed in smokers. IL-1beta was detected in GCF of 95% of both smokers and non-smokers and IL-1ra in all patients. The GCF level of IL-1ra was approximately 1,000-fold that of IL-1beta. The GCF levels of IL-1beta and IL-1ra were high in comparison with those of TNF-alpha and IL-6 determined by the same method in our earlier studies. CONCLUSION: Our observations did not reveal any influence of smoking on the levels of IL-1beta and IL-1ra in GCF.     

上前牙固定式树脂平面导板对白细胞介素-1β和白细胞介素-6的影响

目的：在深覆牙合患者的上前牙区制作前牙固定式树脂平面导板,检测制作导板前后龈沟液量及牙周临床指标的变化,从而推断使用固定式树脂平面导板临床的可行性,为临床应用提供指导。方法：随机选择深覆牙合正畸患者30例,共180颗牙,确定每位患者的实验牙及对照牙,为实验牙制作固定式树脂平面导板,测定不同时期的菌斑指数（plaque index,PLI）、龈沟出血指数（sulcus bleeding index,SBI）、探诊深度（probing depth,PD）、附着丧失（attachment loss,AL）,同时提取龈沟液（gingival crevicular fluid,GCF）,并测定其体积,采用酶联免疫吸附实验对制作导板前后龈沟液中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）的浓度进行检测。结果：与制作导板前相比较,实验牙在制作平面导板后4周PLI、SBI、PD、GCF、IL-1β、IL-6均增加,差异有统计学意义（P〈0.05）;实验牙在制作平面导板后4周,与拆除导板后2周相比较,实验牙PLI、SBI、PD、GCF、IL-1β、IL-6均降低,差异有统计学意义（P〈0.05）;制作平面导板后4周,实验牙与对照牙相比,实验牙的PLI、SBI、PD、GCF、IL-1β、IL-6均增高,差异有统计学意义（P〈0.05）。实验牙在制作导板前与拆除导板后相比较,实验牙PLI、SBI、PD、GCF、IL-1β、IL-6均无明显变化（P〉0.05）;对照牙在制作导板前与制作导板后4周、拆除导板后2周相比较,所有指标均无明显变化（P〉0.05）。在佩戴固定式平面导板的过程中,均未检测到AL。结论：通过多因素检测,制作固定式树脂平面导板可使牙龈发生轻微的炎症,但是这种炎症只是暂时的,导板拆除后牙龈可以很快恢复健康,不会对牙周组织造成永久性的损害,应用于临床是可行的。     

Monitoring periodontal disease status in smokers and nonsmokers using a gingival crevicular fluid matrix metalloproteinase-8-specific chair-side test

BACKGROUND AND OBJECTIVE: With current periodontal diagnostic tools it is difficult to identify susceptible individuals or sites at risk. The aim of this study was to evaluate the efficacy of the matrix metalloproteinase (MMP)-8-specific chair-side dip-stick test in longitudinally monitoring the periodontal status of smoking (S) and nonsmoking (NS) patients with chronic periodontitis, using their gingival crevicular fluid (GCF) MMP-8 concentrations. MATERIAL AND METHODS: Clinical parameters, MMP-8 test results and concentrations were monitored in 16 patients after initial treatment and in 15 patients after scaling and root planing (SRP), every other month, over a 12-mo time period. Progressing and stable sites, and sites with exceptionally high MMP-8 concentrations, were analysed in smokers and nonsmokers. RESULTS: SRP reduced the mean GCF MMP-8 levels, test scores, probing depth (PD), attachment loss (AL) and bleeding on probing (BOP). In sites of periodontal disease progression, the distribution of MMP-8 concentrations was broader than in stable sites, indicating a tendency for elevated concentrations in patients with periodontal disease. The mean MMP-8 concentrations in smokers were lower than in nonsmokers, but in smokers' and nonsmokers' sites with progressive disease, MMP-8 concentrations were similar. Sites with exceptionally elevated MMP-8 concentrations were clustered in smokers who also showed a poor response to SRP. In these sites, the MMP-8 concentration did not decrease with SRP and these sites were easily identified by the MMP-8 test. CONCLUSION: Persistently elevated GCF MMP-8 concentrations may indicate sites at risk, as well as patients with poor response to conventional periodontal treatment (e.g. SRP). MMP-8 testing may be useful as an adjunct to traditional periodontal diagnostic methods during the maintenance phase.     

GCF2/LRRFIP1 promotes colorectal cancer metastasis and liver invasion through integrin-dependent RhoA activation

The precise relationship between GCF2 expression and carcinogenesis has not yet been established. To clarify the metastatic potential of GCF2 in colorectal cancer, HT-29 cells stably suppressing GCF2 expression were injected into the spleens of severe combined immunodeficient (SCID) mice. GCF2 suppression reduced the number of metastatic foci in the liver and reduced fibronectin-induced cell adhesion, migration, and invasion. Downstream from the integrin signaling pathways, GCF2 regulates RhoA interaction with the RGS domain of Leukemia associated RhoGEF (LARG). Altogether, our results suggest that GCF2 plays an important role in colorectal cancer metastasis by regulating RhoA-induced cell adhesion, migration, and invasion.     

钴铬合金烤瓷冠修复后龈沟液AST和ALP水平变化的研究

目的检测钻铬合金烤瓷冠修复后龈沟液天冬氨酸转氨酶（AST）和碱性磷酸酶（ALP）水平变化,探讨钴铬合金烤瓷冠修复对患牙牙周组织的影响。方法选择钴铬合金烤瓷冠修复上颌前磨牙病例18例（共23个烤瓷冠）,分别在修复前和修复后6个月进行临床检查,测定龈沟液的量,并对龈沟液内天冬氨酸转氨酶及碱性磷酸酶进行分析。结果钴铬合金烤瓷冠修复后,患牙的菌斑指数无明显改变,但探诊深度,龈沟出血指数、天冬氨酸转氨酶及碱性磷酸酶水平均比修复前明显升高。结论钴铬合金烤瓷冠修复对患牙牙周组织有一定不良影响。     

GCF2基因片段原核表达载体的构建与鉴定

目的 构建肝癌相关抗原GCF2基因片断(323～1 234 bp)原核表达重组质粒.方法 提取人肝癌组织总RNA,通过反转录酶合成cDNA;根据GenBank公布的GCF2基因cDNA序列设计引物,利用RT-PCR法扩增出人GCF2基因片断(编号2-5a),将此片段连接在克隆载体pGEM-T上进行测序,通过酶切、连接将...     

Measurement of both native and inactivated forms of alpha1 proteinase inhibitor in human inflammatory extracellular fluids

BACKGROUND: Inactivation of the elastase inhibitor, alpha1 proteinase inhibitor (alpha1PI), may be of pathogenic significance in inflammatory diseases like periodontal disease. Two key mechanisms of inactivation appear to be (a) the formation of an alpha1PI-elastase complex and (b) proteolytic cleavage by elastase or other enzymes such as metalloproteinases of host origin or enzymes of bacterial origin. Based on the different heat stabilities of the intact, complexed and proteolytically cleaved forms of alpha1PI, an enzyme-linked immunosorbent assay (ELISA) that allowed the simultaneous measurement of native and inactive forms of alpha1PI was developed. METHODS: The ELISA method described employs a commercially available antibody and represents a rapid, reproducible and sensitive method for studying alpha1PI inactivation in human inflammatory diseases. The assay was applied to normal human plasma and to human extracellular fluids obtained from patients with inflammatory diseases such as adult periodontitis and rheumatoid arthritis. Samples from patients with osteoarthritis, a "non-inflammatory" joint disease, were also studied. RESULTS: The findings expressed as the mean percentage (+/-SD) of the total alpha1PI that was inactivated were as follows: gingival crevicular fluid from adult periodontitis patients: 73.5+/-16.6% (n=12); normal human plasma: 8.4+/-4.9% (n=13); knee-joint synovial fluid (SF) from rheumatoid arthritis patients: 12.5+/-4.5% (n=15); plasma from rheumatoid arthritis patients: 8.0+/-1.8% (n=15); knee-joint SF from osteoarthritis patients: 8.6+/-8.2% (n=14); plasma from osteoarthritis patients: 5.7+/-4.8% (n=14). The results obtained by ELISA were in good agreement with those obtained by the semi-quantitative method of SDS-PAGE and Western blotting. CONCLUSIONS: We have shown that the differential heat stability of alpha1PI may be utilised as the basis for a rapid, sensitive and reproducible ELISA assay of alpha1PI inactivation. In gingival crevicular fluid from periodontal disease patients, alpha1PI is mainly inactivated and the extent of this inactivation is much higher than in inflammatory fluids from other chronic diseases such as rheumatoid arthritis. This assay could be useful in monitoring the progression of periodontal disease.     

Analysis of the relationship between the severity of periodontal destruction and proteoglycan metabolism of gingiva and gingival crevicular flu

BACKGROUND: Although it is well-described that proteoglycans (PGs) are among the major non-collagenous components of the matrix which are degraded during periodontal diseases, the relationship between PG metabolism and seventy of periodontal breakdown, the extent of degradation of PGs together with the resulting end-products, and the elimination pathways of these catabolic end-products is likely to need further clarification. OBJECTIVE: The main aim of the present study was to analyze the possible impact of severity of periodontal destruction on PG metabolism of gingiva and gingival crevicular fluid (GCF). MATERIAL AND METHODS: For this purpose, gingiva and GCF samples obtained from patients (n = 45) exhibiting sites (n = 57) with moderate periodontal breakdown (MP) or severe periodontal breakdown (SP) were analyzed for PG metabolism via spectrophotometric determination of uronic acid levels. Gingiva and GCF samples were obtained from the same sites in every patient to analyze the possible relationship between uronic acid content of gingival tissue and GCF. RESULTS: No significant differences were found in uronic acid levels between sites with MP and SP (p > 0.05). The uronic acid content of GCF and gingiva showed significant overlaps between MP and SP sites and uronic acid levels did not present any constant correlation with the clinical parameters (p > 0.05). In a similar manner, uronic acid content of GCF and gingival tissue was not correlated (p > 0.05). CONCLUSION: The lack of a significant correlation between the uronic acid content of gingival tissue and GCF may suggest that the passage of PG metabolites from gingiva to GCF is likely to be under the influence of multifactorial interactions rather than being linear. As a general measure of PG metabolism, uronic acid levels do not seem to be related with the severity of periodontal destruction and tend to act as different measures when compared to traditional clinical parameters.