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11.
12.
研究了苏乐康、绿茶提取物对~(60)钴γ射线和雌性激素联合诱发叙利亚金黄地鼠胚胎细胞恶性转化的抑制能力,并用脉冲辐解技术,对其抑制机制进行初步探讨。实验结果表明,苏乐康、绿茶提取物对γ射线和雌性激素联合诱发的金黄地鼠胚胎细胞恶性转化的抑制率分别为70.15%(P<0.05)和81.59%(P<0.05),它们的这种抑制能力与其清除超氧阴离子的能力有关, 相似文献
13.
Preeti G. Lal Roopa S. Ghirnikar Lawrence F. Eng 《Journal of molecular neuroscience : MN》1996,29(1):93-104
Glial fibrillary acidic protein (GFAP) is the principal intermediate filament protein found in mature astrocytes. Although
the exact function of GFAP is poorly understood, it is presumed to stabilize the astrocyte’s cytoskeleton and help in maintaining
cell shape. Previous studies from our laboratory have shown that when astrocytes were cocultured with primary Schwann cells
(pSCs), astrocytes became hypertrophied and fibrous with intensely positive GFAP staining and segregated Schwann cells (SCs)
into pockets. In order to understand the functional role of GFAP in this already established astrocyte-SC coculture model,
we generated GFAP-negative cell lines from a GFAP-positive astrocytoma cell line and cocultured both the cell lines with pSCs.
Our studies demonstrate that the GFAP-positive cell line put out processes toward the SCs, whereas the GFAP-negative cells
did not form processes and the majority of the cells remained round. The most significant and interesting finding of this
study, however, is the formation of elaborate processes by SCs when grown in coculture with the astrocytoma cells, unlike
SCs cultured alone, which showed their typical bipolar spindle-shaped morphology. The extent of processes did not seem to
be dependent on GFAP, since SCs cultured with both the cell lines formed similar processes. This coculture model may be useful
in elucidating the factor(s) responsible for the formation of processes by SCs and can be further help in our understanding
of the mechanism of morphological transformation of SCs. 相似文献
14.
Nagata H Numata T Konno A Mikata I Kurasawa K Hara S Nishimura M Yamamoto K Shimizu N 《Pathology international》2001,51(10):778-785
Chronic active Epstein-Barr virus infection (CAEBV) is a syndrome that takes diverse clinical courses and is often associated with lymphoproliferative disorders of T/natural killer (NK)-cell lineage. We describe a patient with CAEBV associated with persistent pharyngeal ulcer, and with subsequent nasal T/NK-cell lymphoma in her neck lymph nodes and nasopharynx. Immunophenotyping of lymphoid cells showed that the lineage of Epstein-Barr virus (EBV)-positive cells in the patient was of NK-cell origin. By means of high-dose recombinant interleukin-2, we established an EBV-positive cell line of NK-cell lineage from her peripheral blood. Southern blot analysis for the number of terminal repeat sequences of EBV detected three NK-cell clones in the patient's lymph node. One of these clones was identical to the established cell line but was not observed in the pharyngeal ulcer, while the other two clones were present in the pharyngeal ulcer. These results suggest that the patient had expansion of the three NK-cell clones, one of which had proliferative capacity in vitro and was involved in the formation of the lymphoma. Moreover, the results suggest that the proliferative capacity of EBV-positive cells can be variable even in a single patient, and this variability may explain the clinical diversity in CAEBV. 相似文献
15.
医学图像序列压缩是远程医疗系统中的重要技术,而运动估计在视频序列压缩中起着关键作用。我们提出了一种改进的正方形-菱形搜索算法来实现医学图像序列的运动估计。这种改进的正方形-菱形算法减少了搜索点数。我们将其应用于小波域的医学图像序列的运动估计,并对数字减影血管造影图像序列(DSA)进行实验。结果表明,改进后的小波域正方形-菱形算法较其他算法精度高。 相似文献
16.
NIH 3T3细胞转化前后细胞骨架及细胞表面纤维粘连蛋白的免疫荧光观察 总被引:1,自引:0,他引:1
本实验用抗纤维粘连蛋白(FN)亲和层析纯抗体和抗管蛋白抗体及鬼笔环肽,以免疫荧光组织化学方法,对NIH3T3细胞转染人肺腺癌细胞系AGZY基因组DNA前后细胞表面FN及细胞内骨架系统进行染色观察。结果表明,细胞在发生转化后,微丝及微管均表现出明显受损,细胞骨架结构不清,呈现为弥散样荧光;细胞表面FN大量减少,仅及正常NIH3T3细胞的1/9,其分布也由细丝形成的网状,变成斑点或斑块状。这一结果进一步证实,细胞恶变是涉及到细胞骨架系统及膜表面糖蛋白变化的复杂过程,并预示这些变化可能就是导致细胞形态发生变化、细胞失去正常生长调控的原因之一。 相似文献
17.
H. Heuer Jörg Sangals 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1998,119(2):224-236
In two experiments the involvement of relative and fixed coordinate systems in visuomotor transformations was examined. The
experimental task required the successive performance of two movements in each trial, which had to “correspond” to different
visual stimuli. One kind of visual display indicated target positions by way of different horizontal positions of a vertical
line on a monitor (position mode), while the other indicated movement amplitudes by way of different lengths of a horizontal
line (amplitude mode). Formal analysis of variances and covariances of successive individual movements led to the conclusion
that in the position mode visuomotor transformations were based on a mixture of relative and fixed coordinate systems, while
in the amplitude mode only a relative coordinate system was involved. Thus, visuomotor transformations can be characterized
as mixtures of different coordinate systems, and their respective weights in the mixtures are task-dependent.
Received: 18 March 1997 / Accepted: 25 September 1997 相似文献
18.
对于眼底图像 ,由于不在正视点取像造成的几何畸变和由于荧光素从血管壁渗漏等造成的灰度畸变 ,几乎是不可避免的。为临床诊断提供丰富信息的需要出发 ,进行多幅眼底图像的拼接是必要的。本文为此提出了几个新的处理方法 ,如基于大窗口的滤波灰度校正方法、基于透视投影原理的几何校正方法与基于小波子空间上的拼接算法 ,均具有效果好和耗时少的优点。 相似文献
19.
The introduction of exogenous DNA into the nuclear genome of Chlamydomonas reinhardtii occurs predominantly via non-homologous (illegitimate) recombination and results in integration at apparently-random loci. Using truncated and modified versions of the C. reinhardtii ARG7 gene in a series of transformation experiments, we demonstrate that homologous recombination between introduced DNA molecules occurs readily in C. reinhardtii, requires a region of homology of no more than 230 bp, and gives rise to intact copies of ARG7 in the nuclear genome. Evidence is presented for homologous recombination between introduced ARG7 DNA and the resident copy of the gene, and for the de-novo synthesis of the ARG7 sequence during transformation. 相似文献
20.
人肿瘤细胞TH2类细胞因子的逆转与NK抗性变化的研究 总被引:3,自引:0,他引:3
目的研究促使人肿瘤细胞中TH2类细胞因子表达向TH0型的逆转及逆转后的肿瘤细胞对NK抗性的变化。方法首先用MTT方法对悬浮培养的肿瘤细胞株DB大淋巴细胞瘤、Karpas淋巴瘤、Michael淋巴瘤、Raji、HL60和K562进行了NK杀伤敏感性的筛选。选择NK不敏感的Karpas淋巴瘤和HL60,用rhIFNγ、rhIL-12和抗IL-10McAb经不同组合对其进行由TH2类细胞因子表达向TH1类细胞因子表达的促逆转研究,并观察促逆转后的肿瘤细胞对NK抗性的变化。结果RT-PCR结果表明,经上述不同细胞因子组合诱导后,Karpas淋巴瘤细胞均从表达TH2类细胞因子为主向TH0型逆转,并且各组逆转后的肿瘤细胞对NK的抗性均有不同程度的减弱。结论TH1类细胞因子(如IFNγ)、TH2类细胞因子拮抗剂(如IL-10单抗)和IL-12不同程度地促进肿瘤细胞表达的细胞因子由TH2型向TH0/TH1型逆转。促逆转后可以改善肿瘤细胞对机体杀伤作用的敏感性,提高抗肿瘤免疫能力 相似文献