Association of TERT gene polymorphisms with clinical benign prostatic hyperplasia in a Chinese Han population of the Northwest region

BackgroundTo investigate the association between single nucleotide polymorphisms (rs10078761, rs12696304, rs2853669, rs16847897, rs2736100, rs10069690) of telomerase gene (TERT) and the risk clinical benign prostatic hyperplasia (BPH) in a Chinese Han population of the Northwest region.MethodsA total of 150 BPH patients and 150 healthy older males from the northwest Chinese Han population were included in this study. The sample size for this unmatched case-control study was estimated by the look-up table method. Meanwhile, the general information and disease data of patients were collected. Age was only collected in healthy control subjects for statistical correction. Genotypes were detected using a multiplex PCR + ligase detection reaction (LDR). Typing results and clinical data were statistically analyzed using multiple linear regression and logistic regression. Pearson correlation was used for Hardy-Weinberg equilibrium.ResultsThe included population is in Hardy-Weinberg equilibrium. There was no significant association between SNP and the risk of BPH by correlation analysis. However, 4 haplotypes (TCTGGT, TCTGTC, TGCCTC, and TGTGTC) were identified as risk factors of BPH by haplotype analysis. The SNP rs2853669 is an independent risk factor for smooth muscle type of hyperplasia. Besides, rs2736100, rs10078761, and rs10069690 which are in linkage disequilibrium are associated with the severity of BPH.ConclusionsPolymorphism of the TERT gene determines the different disease development and pathological manifestations of BPH in the Chinese Han population the Northwest region.     

睫状前动脉的巨微解剖及其临床意义

对30只成人和6只新生儿眼球的睫状前动脉的数目、起点外径、长度、行径及侧支吻合进行观测.上、下、内睫状前动脉多为二支,各占76.7%;73.3%;83.3%.外睫状前动脉多为一支(76.6%).上、下、内、外睫状前动脉的长度分别为5.58土0.44mm;4.79士0.72mm;4.43土0. 76mm;8.00土1.07mm.起点外径分别为0.20mm;士0.04mm;0.I8士0.02mm;0.21土0.03mm.在巩膜表面和虹膜大环处睫状前动脉的侧支之间以及侧支与睫状后长动脉之间相互吻合,这些吻合在眼前节侧支循环方面起一定作用.文中对眼直肌手术时如何减少或避免睫状前动脉损伤等问题做了探讨.     

对单个活态细胞结构与功能多个水平层次的实时测定技术

本文介绍我们发展起来的．用于同时在胞内细胞分子水平、细胞骨架＼细胞膜、以及细胞整体形态三个水平层次上．对单个活态细胞的结构与功能进行无扰、实时、原位检测的系列技术及其有关应用。     

一片8Xc196Mc单片机的便携式多参数监护仪的研制

目的　研制一种便携式多参数生命指征监护仪。方法　以一片 8Xc1 96Mc单片机和一个高分辨率 (6 40× 2 0 0 )的液晶显示器 (LCD)为基础 ,采用信号检测和数据处理技术实现心电、血压、呼吸频率、体温 4个生理参数的实时检测和显示。结果　本系统可动态实时显示所采集的心电波形和其它的参数 ,以及所检测参数 2 4h的趋势图 ,并可通过RS2 32接口与PC机之间进行数据传输。结论　该仪器小型轻便 ,使用灵活 ,有较强的抗干扰能力 ,具有很大的应用价值     

RADIATION INDUCED PROGRESSIVE DECREASING IN THE EXPRESSION OF REVERSE TRANSCRIPTASE GENE OF hEST2 AND TELOMERASE ACTIVITY

Objectites. In order to identify the relationship between telomerase and the biological effect of radiation injury, and investigate the role of human telomerase catalytic subunit gene (hEST2) reverse tranacriptase(RT) seg-ment in the expression of telomerase activity. Methods. Tumor FIeLa cells, KB cells and A431 cells were employed to measure the change in telomeraseactivity after 60Co-ray irradiation at RNA level and protein level. Quantitative PCR and Northern blotting wereused to determine the expression of bEST2 RT segment that encodes seven motifs of the human telomeres, a PCR-besed telomeric repeat amplification protocol (TRAP)was used to assay telomerase activity after exposure toradiation. Results. Both of telomerase activity and the expression hEST2 RT segment were decreased with increasingdosage of radiation. In addition, testing the expression of motifs domain is similar to the measurement of telomerase activity. Conclusion. The detection of the hEST2 BT segment by Northern blotting and quantitative PCR are new methods for testing Uflomerase activity. Furthermore, radiation can cause a dose-dependent decrease in telomerase activity. The effect of radiation on telomerase is one possible reason for the death of cancer ceils after irradiation.     

食管癌中3p24和11p15.5染色体位点潜在抑癌基因的研究

目的：探讨３ｐ２４染色体位点的杂和缺失和１１ｐ１５．６位点的点突变同食管癌之间的相关性,寻找这２个位点中潜在的抑癌基因,并希望通过此研究能为最后确定该基因的功能奠定基础。方法：用ＰＣＲ－ＲＦＬＰ法检测３ｐ２４染色体的３个位点（ＥＡβＭＤ,ＥＡ,βＨ,ＥＡβＲ）上等位基因的杂合缺失情况,ＰＣＲ－ＳＳＣＰ法检测１１ｐ１５,位点的点突变。结果：经ＲＦＬＰ法显示：在３ｐ２４的ＥＡβＭＤ位点,杂合缺失率为７     

DSA单帧双期成像法在肝癌栓塞治疗中的应用

探讨肝癌栓塞治疗中 DSA成像的新方法。 1肝动脉加门静脉像 ,将 mask分别设在肝动脉早、中、晚期和实质期 ,对应的减影像设在门静脉期。 2肝动脉双期像 ,将 m ask设在肝动脉早期 ,对应的减影像设在肝动脉中、晚期和实质期。结果发现 :腹腔动脉 DSA单帧双期成像 37例次 ,肝动脉 DSA单帧双期重建 33例次。提示 :DSA单帧双期成像法有利于指导肝癌栓塞治疗的操作 ,提高超选择性肝内动脉插管成功率 ,缩短手术时间。     

硒对大鼠肝细胞DNA损伤的体内体外研究

应用单细胞凝胶电泳研究了一定剂量的亚硒酸钠对离体和在体大鼠肝细胞 DNA损伤的作用。结果表明 :在2 .185、 4.375、 8.75 0、 17.5 0 0、 35 .0 0 0 μmol/ L 的剂量条件下 ,除 2 .185 μmol/ L 的剂量外 ,其余剂量的亚硒酸钠均可引起离体大鼠肝细胞 DNA损伤。 5、 10、 2 0 μmol/ kg的亚硒酸钠也可引起在体大鼠肝细胞 DNA损伤。亚硒酸钠引起的离体和在体大鼠肝细胞 DNA损伤均存在明显的剂量 -反应关系。研究结果提示 ,一定剂量的亚硒酸钠能引起离体和在体大鼠肝细胞 DNA损伤     

抗前列腺特异抗原单链抗体/人羧肽酶A融合基因的构建及表达

目的 构建抗前列腺特异抗原（PSA）单链抗体（scFv)/人羧肽酶A(hCPA)融合基因,为前列腺癌的抗全导向酶-前体药物疗法奠定基础。方法 在已克隆抗PSAscFv和hCPA基因的基础上,用加端PCR分别在scFv基因的3′端和hCPA基因的5′端加上部分连接肽（linker)基因序列,回收后混合。应用重叠延伸拼接法,将抗PASscFv基因和hCPA基因通过linker序列串联为scFv/hCPA融合基因;并将构建的融合基因克隆到融合表达载体pGEX-4T-1中进行表达。结果 序列测定结果表明,构建的抗PSAscFv/hCPA融合基因中scFv和hCPA序列均正确,scFv和hCPA间有18bp的linker序列,推导的氨基酸序列为GSGGSG,与设计的序列相符,融合基因经IPTG诱导表达重组蛋白并以SDS-PAGE分析,在Mr为94000左右出现一条新生蛋白带,表达量约占菌体总蛋白的34%。结论 成功构建并原核表达了抗PSAscFv/hCPA融合基因。