Chronic exposure to dibromoacetic acid, a water disinfection byproduct, diminishes primordial follicle populations in the rabbit.

To determine if dibromoacetic acid (DBA) affects ovarian folliculogenesis, four groups of female Dutch-belted rabbits were exposed daily to 0, 1, 5, or 50 mg DBA/kg body weight in drinking water beginning in utero from gestation day 15 throughout life. Functionality of the endocrine axis was assessed by measuring serum concentrations of gonadotropins following an im injection of 10 microg GnRH at 12 (prepubertal; n = 6/dose group) and 24 (postpubertal; n = 10/dose group) weeks of age. A day after GnRH challenge, number of ovulation sites and ovarian weights were determined at necropsy. Left ovaries were processed for histopathology, serially sectioned at 6 microm, and every twelfth section stained with hematoxylin and eosin was evaluated. All healthy follicles were categorized as primordial, primary, small preantral, large preantral, or small antral follicles. The area of each section evaluated was measured and the number of follicles in each category expressed per mm2 unit area. In prepubertal animals, DBA caused a reduction in number of primordial follicles (p < 0.05) and total healthy follicles (p < 0.05) at 50 mg/kg dose level. In adult animals, there were fewer primordial follicles in both the 5 (p < 0.01) and 50 (p = 0.1) mg/kg dose groups. No profound changes in gonadotropin profiles were observed. Although chronic exposure to DBA did not appear to have an effect on late follicular development or ovulation, DBA did reduce the population of primordial follicles. The long-term health consequences of diminished primordial follicles are unknown, but it is very likely that reproductive senescence would occur earlier.     

PGC-1α与卵巢功能及相关研究进展

过氧化物酶体增殖物激活受体γ共激活因子1α(peroxisome proliferator-activated receptor gamma coactivator 1 alpha,PGC-1α)属于人体内一种重要的核转录辅助激活因子,是线粒体生成的关键调节因子,通过与核受体及转录因子的相互作用参与线粒体的生物合成、适...     

中医药诱发排卵治疗不孕症研究近况

近年来,多囊卵巢综合症、子宫内膜异位症、高泌乳素血症等疾病引发的排卵障碍已构成女性不孕症的重要原因之一。中医认为,此类不孕症病位在肾,涉及肝、脾,病机关键在于肾阳不足,病理因素有瘀血、痰湿等,因其病因繁多,病机复杂,临床治疗较为困难。西医促排卵治疗单独使用临床疗效不理想,中医药主张从中医基础理论出发,辨证论治,将整体和局部有机结合,综合运用中药内服、针灸、外用、理疗等方法诱发排卵,标本同治,从而提高排卵率和临床妊娠率。     

多囊卵巢综合征影响因素分析

目的 寻找与多囊卵巢综合征发病有关的因素.方法 采用病例对照研究,调查2005年2月～2006年4月南京医科大学第一、第二附属医院不孕症门诊患者105例及同期非多囊卵巢综合征的就诊患者210例资料,进行单因素分析.结果 多囊卵巢综合征发病的单因素和多因素分析结果:家族遗传史、初潮月经紊乱、失眠、打鼾、职业、室内装潢史,两组比较差异有显著性.结论 家族遗传史、初潮月经紊乱、失眠、打鼾、职业、室内装潢史是多囊卵巢综合征的高危因素.     

促排卵汤联合生活方式干预对多囊卵巢综合征妇女血清SAP、Apelin与IL-18水平的影响

目的 研究促排卵汤联合生活方式干预对多囊卵巢综合征妇女血清SAP、Apelin与IL-18水平含量的影响.方法 选择2016年1月—2016年12月就诊的多囊卵巢综合征患者80例,分为2组各40例.其中,试验组患者在对照组常规理疗的同时添加促排卵汤联合生活方式干预,比较2组患者血清SAP、Apelin与IL-18水平含...     

秩边透水道为主针刺治疗多囊卵巢综合征疗效观察

目的:比较秩边透水道为主针刺与常规针刺治疗多囊卵巢综合征的疗效差异.方法:将101例患者按照随机数字表法随机分为观察组(49例)和对照组(52例).观察组选取秩边透水道为主穴,并随证配穴,采用长175 mm的芒针与矢状面呈20°夹角的方向刺入秩边穴,进针深度100～115 mm,透达水道穴,使针感直达病所;对照组与观察组取穴相同予以常规针刺法.两组患者均不分月经前后及经期与否,连续针刺治疗,每日治疗1次,15次为一疗程,连续治疗5个疗程.观察两组患者治疗前后基础体温情况、性激素水平,并比较两组临床疗效.结果:观察组痊愈率为65.3％(32/49),有效率为91.8％(45/49),明显优于对照组的48.1％(25/52)、63.5％(33/52)(均P＜0.01).观察组基础体温典型双相及不典型双相例数均多于对照组,单相例数少于对照组,差异有统计学意义(P＜0.05);观察组治疗后卵泡刺激素(FSH)、黄体生成素(LH)、LH/FSH、睾酮(T)均明显下降(P＜0.01,P＜0.05),对照组治疗后LH、T亦明显降低(P＜0.01,P＜0.05),观察组治疗后FSH、LH、LH/FSH、T较对照组降低明显(P＜0.05,P＜0.01).结论:秩边透水道为主针刺能明显改善多囊卵巢综合征患者的基础体温,调节生殖内分泌激素水平,其疗效均优于常规针刺.     

排卵汤联合枸橼酸氯米芬治疗多囊卵巢综合征不孕症46例临床观察

目的观察排卵汤联合枸橼酸氯米芬(CC)治疗多囊卵巢综合征(PCOS)不孕症临床疗效。方法将139例PCOS不孕症患者随机分为3组。对照1组46例予CC治疗,对照2组47例予CC、注射用尿促性素(HMG)治疗,治疗组46例予排卵汤联合CC治疗。观察子宫内膜厚度、排卵率、妊娠率、未破裂卵泡黄素化(LUF)、多胎率及流产率等。结果治疗组流产率、多胎率均低于对照1组、对照2组(P0.05);治疗组子宫内膜厚度为8~12 mm患者百分率及妊娠率高于对照1组、对照2组(P0.05)。结论排卵汤联合CC治疗PCOS患者可以获得满意的周期妊娠率,并降低并发症的发生。     

Malignant Struma Ovarii: Two Case Reports and a Review of the Literature

Struma ovarii consists of thyroid tissue derived from germ cells in a mature teratoma. Malignant transformation is very rare, with clinically evident metastatic disease reported in approximately 20 cases. The rarity of this disease renders evaluation of treatment modalities difficult. There is evidence that these tumors behave like their thyroid counterparts, and cytoreductive surgery followed by ablation with radioactive iodine has been advocated. We report the diagnosis and treatment of 2 patients with metastatic malignant struma ovarii treated with a combination of surgery and radiation therapy.     

Assessment of ovarian vascularization in the polycystic ovary by three-dimensional power Doppler ultrasonography

Objective. To assess whether there are differences in ovarian echogenicity and vascularization as assessed by three-dimensional power Doppler angiography (3D-PDA) between women with polycystic ovaries (PCO) and women with normal ovaries (NO).

Methods. Eighty-three women were classified into two groups according to the 2003 Rotterdam consensus criteria. The NO group comprised women (n = 45) with regular menstrual cycles and proven fertility, whereas the PCO group comprised women (n = 38) with oligo-anovulation, clinical and/or biochemical features of hyperandrogenism, and polycystic ovary morphology at two-dimensional ultrasound. All women were evaluated by means of 3D-PDA. The parameters studied in both groups were follicle number per ovary (FNPO), ovarian volume (OV), mean gray value (MG) and three vascular indices: vascularization index (VI), flow index (FI) and vascularization flow index (VFI).

Results. The PCO group showed a higher mean OV as well as FNPO. No differences in MG, VI, FI and VFI were found between the groups.

Conclusions. 3D-PDA indices are not useful for discriminating between normal and polycystic ovaries.