高效液相色谱法测定人子宫、输卵管及血清中氧氟沙星浓度

目的建立人组织及血清中氧氟沙星含量的HPLC测定方法。方法采用Hypersil C₁₈柱分离,流动相为乙腈-0.01 moL·L^-1磷酸盐缓冲液-0.5 moL·L^-1四丁基溴化铵(9∶91∶4, pH 2.5),流速1.0 mL·min^-1,在UV 294 nm处测定；样品经匀浆或用液氮冷冻研磨至粉末状，用1%曲拉通-100溶解，乙酸乙酯-异丙醇（9∶1）提取，HPLC测定。结果 氧氟沙星浓度在0.2～8.0 μg·mL^-1与峰高比呈线性关系,最小检测限40 ng·mL^-1,用本法对20例妇产科病人进行药物浓度测定,取得了满意的结果。结论此方法适用于人体组织中氧氟沙星浓度的测定，口服后氧氟沙星在人体组织中有良好的分布。     

用尿药法考查胃舒平对氧氟沙星药物动力学的影响

目的：考查胃舒平对氧氟沙星药物动力学的影响。方法：用尿药浓度法测定６名志愿者单剂量口服氧氟沙星和口服氧氟沙星时合用胃舒平后的尿药浓度。结果：合用胃舒平时氧氟沙星的吸收达峰时间推迟（Ｐ＜０．０１），其药动学参数合用前后Ｔｍａｘ分别为１．６２ｈ，２．９０ｈ；Ｔ１／２分别为６．７６ｈ，６．４６ｈ；排泄率分别为８７％，８４％。结论：氧氟沙星与胃舒平联用后吸收明显减少，应避免联用     

用氧氟沙星滴耳液出现听力下降1例

病人 ,男性 ,38a ,因慢性化脓性中耳炎急性发作 ,用氧氟沙星滴耳液 (每瓶 5mL ,含氧氟沙星15mg)滴耳 ,每次 3滴 ,每日 3次 ,当滴到第 2瓶第3次时引起听力损失     

氧氟沙星片剂的尿排泄率测定

目的 :测定氧氟沙星片剂经尿液的排泄率。方法 :对 7名健康男性志愿者单次给氧氟沙星片剂 2 0 0mg ,采用紫外分光光度法测定该药尿液中的浓度 (测定波长为 3 3 1nm)。 结果 :氧氟沙星片剂经尿液的排泄率为82 .77± 2 .65 %。结论 :用该法测定氧氟沙星片剂经尿液的排泄率 ,其方法简便快速 ,结果可靠     

奥硝唑和氧氟沙星治疗慢性盆腔炎的效果分析

目的探讨慢性盆腔炎患者行奥硝唑联合氧氟沙星治疗的临床效果。方法将本院于2017年4月—2018年4月期间接收的慢性盆腔炎患者52例纳入本次研究中,以随机数字表法的形式将其平均分为观察组和对照组,每组各为26例。对照组行奥硝唑治疗,观察组在此基础上加行氧氟沙星治疗,对比两组治疗后的指标。结果(1)治疗后观察组炎性因子指标和生活质量优于对照组,组间对比有统计学意义(P<0.05)。(2)对照组各项指标缓解用时长于观察组,组间比较差异具有统计学意义(P<0.05);(3)观察组总有效率92.3%高于对照组69.2%(P<0.05)。结论奥硝唑联合氧氟沙星治疗慢性盆腔炎治疗后,临床指标显著改善,总有效率提升。     

A Review of the Antimicrobial Activity of the Fluoroquinolones

Summary

The evolution of the fluoroquinolones is described, and structure-activity relationships outlined. The in-vitro antimicrobial activities of ciprofloxacin, enoxacin, norfloxacin, ofloxacin and pefloxacin against a wide range of organisms are critically reviewed. In-vitro factors influencing fluoroquinolone activity are discussed. Reports of the acquisition of resistance to the fluoroquinolones are evaluated. Finally, possible future directions for this group of antibiotics are discussed.     

喹诺酮类药物的热稳定性及其热分解非等温动力学研究

目的　研究喹诺酮类药物的热稳定性及热降解,建立动力学方程。方法　用TG-DTG热重仪测定药物的热解曲线，用Achar微分法和Coats-Redfern积分法确定热分解函数。结果　根据TG-DTG热解曲线和红外图谱推断出依诺沙星、诺氟沙星、盐酸芦氟沙星和氧氟沙星等4种喹诺酮类药物的热分解过程并求出其热解动力学参数活化能E、反应级数n和指前因子A及动力学补偿参数。结论　热稳定性为盐酸芦氟沙星>依诺沙星>氧氟沙星>诺氟沙星,它们的热分解过程相同;4种药物胶囊的稳定性均大于各自原药;诺氟沙星、依诺沙星、氧氟沙星的热解机理函数相同，动力学方程为dα/dt=Ae^-E/RT(1-α)²，盐酸芦氟沙星为dα/dt=Ae^-E/RT(1-α)³。     

氧氟沙星联合丁胺卡那霉素治疗耐多药肺结核的疗效分析

目的观察氧氟沙星联合丁胺卡那霉素在治疗耐多药肺结核治疗中的作用。方法将耐多药肺结核患者71例随机分为2组:治疗组(38例)采用卡那霉素联合利福平、结核清及吡嗪酰胺、乙胺丁醇,疗程18个月。结果患者完成化疗疗程时;治疗组痰菌阴转率89.5%,优于对照组54.5%,(P<0.01);治疗组病灶吸收率94.7%,优于对照组57.5%,治疗组空洞闭合率71.9%,也优于对照组35.3%,(P<0.05)。结论氧氟沙星联合丁胺卡那霉素治疗耐多药肺结核痰阴转高,病变吸收好,药物不良反应低,值得在临床上推广应用。     

Simultaneous screening for marbofloxacin and ofloxacin residues in animal-derived foods using an indirect competitive immunoassay

A broad-specificity immunoassay was established to monitor marbo?oxacin (MBF) and ofloxacin (OFX) residues in raw milk and porcine muscle samples. The main influential parameters in the assay buffer were investigated to improve the assay performance. The cross-reactivities (CRs) were lower than 0.25%, except for OFX (41.38%). The quantitative working ranges for MBF and OFX based on fortified matrices ranged from 0.11 to 30.85?ng?mL^?1, with a 50% inhibitory concentration of 0.76±0.12?ng?mL^?1 for MBF and 2.70±0.28?ng?mL^?1 for OFX. The detection limits of enzyme-linked immunosorbent assay (ELISA) ranged from 0.053 to 0.358?ng?mL^?1 in spiked samples. The mean recoveries were in the range of 80.52–95.46%, less than 12.06% of the coefficient of variation. A good correlation between the ELISA results and those from instrument methods demonstrated the practical application of the novel ELISA. Therefore, the immunoassay proposed is feasible for MBF and OFX analysis in animal-derived foods.     

Comparison of Graphitic Carbon Nitrides Synthetized from Melamine and Melamine-Cyanurate Complex: Characterization and Photocatalytic Decomposition of Ofloxacin and Ampicillin

Graphitic carbon nitride (g-C₃N₄, hereafter abbreviated as CN) was prepared by the heating of melamine (CN-M) and melamine-cyanurate complex (CN-MCA), respectively, in air at 550 °C for 4 h. The specific surface area (SSA) of CN-M and CN-MCA was 12 m² g⁻¹ and 225 m²g⁻¹ and the content of oxygen was 0.62 wt.% and 1.88 wt.%, respectively. The band gap energy (E_g) of CN-M was 2.64 eV and E_g of CN-MCA was 2.73 eV. The photocatalytic activity of the CN materials was tested by means of the decomposition of antibiotics ofloxacin and ampicillin under LED irradiation of 420 nm. The activity of CN-MCA was higher due to its high SSA, which was determined based on the physisorption of nitrogen. Ofloxacin was decomposed more efficiently than ampicillin in the presence of both photocatalysts.