Numerical aberrations of chromosomes 11 and 17 in colorectal adenocarcinomas

Fluorescence in situ hybridization (FISH) is an easy and efficient means of measuring numerical chromosome aberrations in the interphase nuclei of solid tumors; however, the correlation between numerical chromosome aberrations and the clinical stage of solid tumors remains unknown. Thus, in the present study, we investigated the relationship between numerical chromosome aberrations and clinicopathologic features in colorectal adenocarcinomas. FISH was applied to surgically resected colorectal cancer samples from 45 patients to evaluate the numerical aberrations of chromosomes 11 and 17. The mean age of the patients was 65.1 years, and they comprised 13 women and 32 men. According to Dukes' classification, 5 patients were categorized as stage A, 21 as stage B, 10 as stage C, and 9 as stage D. Histologically, 18 of the samples were lymph node metastasis-positive. FISH revealed numerical aberrations of chromosome 11 in 27 out of the 45 patients (60%), and those with a lower chromosome 11 number had a significantly lower incidence of lymph node metastasis (P<0.05). Chromosome 17 proved to have numerical abnormalities in 33 of the 45 patients (73%), and those with a higher chromosome 17 number had more DNA aneuploidy (P<0.005). This is the first report to reveal the relationship between monosomy 11 and lymph node metastasis in colorectal adenocarcinomas.     

脉搏波与生理病理变化关系的仿真研究

为探讨脉搏波中所含生理病理信息的机理，采用一个由１２０个数字计算单元组成的人体心血管系统血液动力学分布式仿真模型，对血液粘度和血管弹性的变化所致桡动脉脉搏波的发迹以及心力衰竭、高血压、动脉硬化３种病状所致脉搏波的改变趋势进行了数字仿真研究，取得了初步煌结果。     

流体化学位的MonteCarlo模拟

采用耦合位能函数伞形法解决了流体化学位模拟中Ｗｉｄｏｍ试验粒子法的高密度困难。新方法的实现相当容易，而且同样适用于ＭＤ模拟。通过对Ｌｅｎｎａｒｄ－Ｊｏｎｅｓ流体的模拟，表明这一方法比原始的Ｗｉｎｄｏｗ试验粒子法具有更高的计算效率，其结果更加可靠。     

Effects of Chinese Herb Medicine on Improving the Circulation of Rabbits Under Simulated Weightlesness

为了探讨中药在改善失重状态下血循环的作用，本实验通过建模和辨证、筛选药物、确定用药剂量、药理实验及丹黄合剂的毒副作用等的研究，选出了两种对模拟失重兔血循环紊乱有良好改善作用的中药即丹黄合剂和川穹。在此基础上，对模拟失重动物模型、航天血瘀征、中药作用机理等几个问题进行了分析讨论     

Effectiveness of Computer-Aided Removable Partial Denture Design

Purpose Computer programs have been developed for removable partial denture (RPD) design, but their educational impact has never been investigated. This study measured the effectiveness of computer-based RPD design simulations in a traditional RPD design course for second-year dental students. Materials and Methods Thirty-six students were randomly assigned to four groups, two simulation and two control groups, at the beginning of the RPD design module. A cross-over design compared the two strategies during laboratory exercises. First, the simulation group worked with the simulation, while the control group met in small-group seminars with faculty. Post-test 1 was given, and then students switched teaching methods and post-test 2 was given. Each post-test had two parts. Part 1 involved designing an RPD based on specific patient criteria. Part 2 involved choosing the more correct of the two designs. Results Results showed a significant difference (p= .0072) in two of four test groups. Although it was an initial evaluation, the computer simulation was shown to be equivalent to faculty-led small-group seminars. Students also responded positively to a questionnaire on their perceived effectiveness of the simulations. Conclusions The results suggest that RPD design can be taught as effectively with computer-based simulations as with faculty-led seminars.     

模拟失重对兔红细胞形态及生成的影响

为探讨微重力下血液变化机理，观察了头低位兔血液红细胞形态及骨髓造血功能的变化，发现头低位组兔血液中的畸形红细胞增多，骨髓中出现了稍密集的造血细胞，网状细胞核孔增多，内质网扩张，幼稚红细胞进入血窦，巨噬细胞吞噬幼稚红细胞，血窦内皮细胞空泡化等现象。实验结果表明，短期模拟失重以地红细胞形态和骨髓造血功能有一定的影响。     

两种气功对模拟失重对抗能力的比较研究

为了比较两种气功对模拟失重的对抗能力，观察了７７名男性青年被试者。其中１０名为未练过任何气功的对照组；１７名为大专气功二年级学生，修练动功１年多为气功１组。５０名相当于大专的气功师资班学生练的是智能气功，为气功Ⅱ组。以受试者头低位（─３０°）３０ｍｉｎ作为模拟失重的急性刺激，观察心搏量、下肢血流、血压、心率、膻中和劳宫穴的皮肤温度变化。结果表明，在头低位（─３０°）的刺激下，气功组的下肢血流增加，明显高于对照组，且具有统计学意义。而气功Ⅱ组又好于气功Ⅰ组。对照组的舒张血压升高，达１４％，而气功Ⅰ组只增加４％，气功Ⅱ组则未有增减。同时，气功组的膻中、劳宫穴皮肤温度也明显高于对照组。证明，气功组与对照组相比，有较高的对抗模拟失重能力。两组气功组相比，则气功Ⅱ组对模拟失重有更好的对抗能力。     

赫坎按蚊种团中5种4株的数值分类

本文报告应用系统聚类分析和主成分分析法对赫坎按蚊种团中的中华按蚊4个地理株以及嗜人按蚊、贵阳按蚊、昆明按蚊和凉山按蚊进行分析的结果。并对分析的具体步骤作了简单介绍。     

发声空气动力学问题比较研究

作者从声带多质量模型仿真、活体犬动物实验和人体发声声门图测量多方面对声带流体诱发振动及其空气动力学进行了比较研究。结果表明:①人与犬呼气式和吸气式发声声带振动机理是一致的。但因发声动力与负载条件、声带尺寸及力学特性等因素的不同,使振动模式存在差导;②跨声门压(或声门气流量)与声门阻抗的关系不仅影响振动模式(如胸声、气声和压紧嗓音),而且确定了声门气流量是否足以诱发声带振动;③采用3种方法对相同发声方式的研究结果是一致的。     

Chromosomal numerical abnormality profiles of gastrointestinal stromal tumors

BACKGROUND: Biological variations in and the heterogeneity of gastrointestinal stromal tumors (GISTs) are well known, but chromosomal numerical abnormality (CNA) has not been fully examined especially in this context. The aim of this study is to test CNA as a possible biological predictor of biological behavior of GISTs. METHOD: We applied microwave-assisted FISH protocol to pathological archives of GIST tumors displaying different clinical features to characterize the CNA profile of these tumors. A panel of 18 centromere enumeration probes (CEP) and 24 bacterial artificial chromosome (BAC) or P1-derived artificial chromosome (PAC) probes containing genes like Aurora kinases (AURKs) and other candidate genes involved in human carcinogenesis were used. CNA profiles, histopathological risk categorization and Ki-67 labeling indexes of 23 primary and/or metastatic GIST tumors of 12 subjects (both primary and metastatic in 7 subjects) were compared between primary GIST with and without metastases, and between metastatic and primary portions in 7 individuals. RESULTS: CNA in the primary sites was more extensive in the GISTs with recurrence and metastasis than in those without, especially as to the loss of chromosome 20 and genomic imbalance of AURKA-containing BAC probe on 20q in the cases with metastasis. The consistent loss of one allele of chromosome 14q was also noted. Interestingly, both primary and metastatic tumors in identical individuals had similar CNA profiles. CONCLUSION: The extent of CNA differed between GISTS with and without recurrence or metastasis; thus, FISH analysis of specimens from the primary sites may predict the biological behavior of this tumor.