HSP70、Galectin7在放疗敏感性不同宫颈癌组织中表达的研究

目的:观察HSP70和Galectin7在对放疗敏感性不同宫颈癌组织中的表达及临床病理学意义。方法:收集治疗前早期宫颈癌组织标本,病理诊断均为中分化鳞状细胞癌,置于-80℃超低温冰箱保存。在放疗后,根据WHO实体瘤放疗疗效判断标准,从中选择对放疗敏感的5例(高敏感组)和对放疗不敏感的5例(低敏感组),提取其组织总蛋白,进行双向凝胶电泳和质谱分析鉴定差异表达蛋白,分别应用Western Blot和免疫组织化学方法检测组织中蛋白的表达。结果:建立了分辨率高的宫颈癌放疗高敏感组和低敏感组的双向凝胶电泳图谱,差异表达蛋白经鉴定得到高敏感组高表达的Galectin7蛋白以及低表达的HSP70蛋白。Western Blot检测结果显示Galectin7在高敏感组的表达强于低敏感组,HSP70在低敏感组的表达强于高敏感组。免疫组化结果显示,HSP70在高敏感组的阳性表达率为21.7%(13/60),低敏感组的阳性表达率为85.7%(30/35),差异有统计学意义(χ2=36.5997,P<0.0001);Galectin7在高敏感组的阳性表达率为91.7%(55/60),在低敏感组中的阳性表达率为40%(14/35),差异有统计学意义(χ2=29.6853,P<0.0001),并且Galectin7在高敏感组的表达强度高于低敏感组,HSP70在低敏感组的表达强度高于高敏感组,差异均有统计学意义。结论:HSP70和Galectin7的表达与宫颈癌放疗敏感性有关。     

Insulin resistance is not coupled with defective insulin secretion in primary hyperparathyroidism

Aims An increased frequency of both impaired glucose tolerance and Type 2 diabetes mellitus (DM) has been reported in primary hyperparathyroidism (pHPT), thus we sought to investigate insulin sensitivity and insulin secretion in a large series of pHPT patients. Subjects and methods One hundred and twenty‐two consecutive pHPT patients without known DM were investigated [age (mean ± sd ) 59.3 ± 13.6 years, body mass index (BMI) 25.7 ± 4.2 kg/m²; serum calcium 2.8 ± 0.25 mmol/l; PTH 203.2 ± 145.4 ng/l]. Sixty‐one control subjects were matched, according to the degree of glucose tolerance, in a 2 : 1 patient:control ratio. Fasting‐ and oral glucose tolerance test‐derived estimates of insulin sensitivity and secretion were determined by means of the quantitative insulin sensitivity check index (QUICKI) and the insulin sensitivity index (ISI) composite. Results Both the QUICKI and ISI composite were lower in pHPT patients than control subjects (P < 0.03 and P < 0.05, respectively) after adjusting for age, systolic blood pressure and BMI. Conversely, all insulin secretion estimates were significantly increased in pHPT patients than in control subjects (P < 0.04 and P < 0.03, respectively) and after adjusting for age, systolic blood pressure and BMI. Log serum calcium levels were negatively associated with the QUICKI and log ISI composite (R = ?0.30, P = 0.001; R = ?0.23, P = 0.020, respectively) in pHPT patients. Serum calcium levels significantly and independently contributed to impaired insulin sensitivity in multivariate analysis (QUICKI as dependent variable: β = ?0.31, P = 0.004, R² = 0.15; log ISI composite as dependent variable: β = ?0.29, P = 0.005, R² = 0.16). Conclusions Our study confirms a reduction in both basal and stimulated insulin sensitivity in primary hyperparathyroidism, in spite of increased insulin secretion. Moreover, our data show for the first time a significant relationship between hypercalcaemia and insulin sensitivity in this condition.     

新生儿呼吸机相关肺炎的临床分析

目的：了解新生儿呼吸机相关肺炎（VAP）的常见病原菌和相应敏感药物。方法：对本院20例确诊为VAP的新生儿进行下呼吸道分泌物细菌培养和药物敏感试验。结果：20例患儿细菌培养均为阳性，共培养出细菌20株，其中铜绿假单胞菌10株、肺炎克雷白菌5株、大肠杆菌3株、表皮葡萄球菌2株，敏感抗生素依次为环丙沙星、丁胺卡那霉素、头孢三嗪。结论：常见病原菌为铜绿假单胞菌、肺炎克雷白菌和大肠杆菌，首选治疗药物为环丙沙星。     

军队中主要精神疾病筛查量表修订后在精神科门诊中的初步应用

目的 探讨台湾学者根据MMPI构建的29条目自评量表在精神科门诊中的应用价值。方法 翻译并对台湾学者构建的29条目自评量表进行适当修订，进而在精神科门诊中加以应用，将自评结果同最终诊断结果进行比较，应用尤登指数确定最佳截断点，进一步评价该自评量表在初步诊断中的应用价值。结果 136人按要求完成了量表填写，结果发现该自评量表灵敏度，特异度均较理想，可为精神疾病的诊断提供有用线索，有效的缩短就诊时间，记分方法简单，填表时间短，便于在门诊中应用，具有较大的实用价值。本研究应用尤登指数确定了新的最佳截断点，精神分裂症，抑郁症，噪狂症各自的最佳截断点分别是2分，2分，3分。结论 经过修订的29条目自评量表简便，快捷，实用，成本低，可用于在精神科门诊中初步诊断病人，有效的节约就诊时间，更充分的利用现有的医疗资源。     

Immunological reaction to audiovisual stimulation in healthy subjects

Acoustic and light stimulation of thecorresponding analyzers at 1–13 Hz stimulates T-cell immunity. Immunological reaction to audiovisual stimulation depends on the initial level of adaptation stress and changes in the actual self-evaluation after exposure. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 128, No. 8, pp. 204–206, August 1999     

Research highlights from the literature

Atrial fibrillation is considered a relatively benign condition; however, hemodynamic compromise and excessive embolism and stroke risk are major concerns. Pulmonary vein ablation close to the left atrium using radiofrequency catheters is becoming increasingly popular in the treatment of atrial fibrillation. A recent study suggests that the therapeutic response to pulmonary vein ablation is explained by modulation of adjacent autonomic structures rather than interruption of “electrical circuits” within the artery wall. Two independent studies in patients who survived a myocardial infarction further support the idea that impaired cardiac autonomic function may have utility in predicting risk of future cardiovascular events or death. Perhaps, patients identified in this fashion may benefit from more aggressive treatment—a hypothesis needs to be tested in prospective trials.     

拜新同对高血压伴脑腔隙梗死患者血脂糖耐量和胰岛素敏感性的影响

目的：评价拜新同的降压效果及其对高血压伴脑腔隙梗死患者血脂、糖耐量和胰岛素敏感性的影响。方法：４７例高血压伴脑腔隙梗死患者给予拜新同（拜耳公司），剂量３０～６０ｍｇ／ｄ，治疗６周，另外２２例服用卡托普利２５～５０ｍｇ，每天３次。治疗前后所有参与者都进行血压监测和血清脂质以及口服葡萄糖耐量试验（ＯＧＴＴ）。结果：两组降压效果拜新同较卡托普利为优。并对甘油三酯、糖耐量和胰岛素敏感性有较好的影响。结论：     

中老年人黄斑光敏感度下降的原因分析

目的 :探讨中老年人黄斑光敏感度下降的原因。方法 :采用国产APS -60 0 0B型自动视野分析仪 ,对 14 0例 2 80眼的中老年人进行 0°～ 3 0°中心视野全阈值检测。其中老年黄斑变性组 60眼 ,青光眼组 5 6眼 ,糖尿病组 5 0眼 ,高血压组 44眼 ,分别与对照组 70眼比较。结果 :老年黄斑变性组、青光眼组和糖尿病组的黄斑光敏感度均有不同程度的下降 ,而高血压组与对照组相比差异无显著性。结论 :除年龄因素外 ,老年黄斑变性、青光眼和糖尿病可能是造成中老年人黄斑光敏感度下降的重要原因。其发病机理可能与细胞的凋亡、微循环障碍和血液流变学的改变有关。     

The role of CT and NMRI in neurosurgical diagnosis

Summary A brief survey is given of the state of art of NMRI in neurosurgical diagnosis and its advantages and disadvantages in comparison to CT scanning are discussed. Specificity and sensitivity of these methods are also considered.     

High contrast and low contrast visual acuity in age related macular degeneration*

Low contrast Bailey-Lovie visual acuity (LCBL) and high contrast Bailey-Lovie visual acuity (HCBL) were measured in subjects with age-related macular degeneration (ARM) and subjects with normal visual function. Low and high contrast visual acuities and the difference between low and high contrast acuities were significantly different for the ARM group compared with the normals, confirming previous findings of disruption to contrast discrimination in ARM. The high contrast Bailey-Lovie chart was found to be a useful screening device for macular degeneration using a pass/fail criterion of 6/7.5. Pre-ARM (PARM) subjects can be defined as those with drusen and/or pigmentary disturbance and high contrast acuity of better than 6/7.5. However, the low contrast chart used at normal clinical illuminances is of no additional diagnostic value in ARM.