Effects of olopatadine in limited scleroderma with peripheral eosinophils

Scleroderma and eosinophilia often occur together, though the pathogenesis is unclear. We investigated the effect of olopatadine hydrochloride in a series of cases of limited scleroderma (LS). Ten patients with LS and positive eosinophil counts (LSE) were enrolled (average age, 85 years; six men and four women). Serum concentrations of the anti‐Scl‐70 antibody were positive. Olopatadine hydrochloride was prescribed at 10 mg/day for 3 weeks. Serum concentrations of the anti‐Scl‐70 antibody significantly decreased, but changes in eosinophil numbers and percentages in peripheral blood were not significant. Factor analysis suggested a correlation between serum concentrations of the anti‐Scl‐70 antibody and complement C⁴. Olopatadine could be effective in reducing anti‐Scl‐70 antibodies in the elderly with LSE.     

过敏性休克猝死豚鼠肺组织内嗜酸性粒细胞趋化因子的变化及法医学意义

目的 探讨豚鼠过敏性休克猝死后嗜酸性粒细胞趋化因子(Eotaxin)在肺组织内的表达及法医学意义.方法 12只健康豚鼠随机分为对照组和过敏性休克模型组(n=6).以人血清致敏法建立过敏性休克豚鼠模型,而对照组注射生理盐水.免疫组化法测定Eotaxin在正常豚鼠和过敏性休克模型豚鼠肺组织内蛋白表达.结果 模型组豚鼠肺组织内Eotaxin表达阳性表达显著高于正常对照组,差异有统计学意义(P<0.05).结论 在过敏性休克发生时Eotaxin在肺内阳性表达显著增加,为过敏性休克的判定提供辅助诊断依据.     

Effects of xiao-qing-long-tang (XQLT) on bronchoconstriction and airway eosinophil infiltration in ovalbumin-sensitized guinea pigs: in vivo and in vitro studies

BACKGROUND: Xiao-qing-long-tang (XQLT sho-seiru-to), a traditional Chinese medicine, has been used to treat patients with bronchial asthma in Oriental countries for several centuries. However, the therapeutic mechanisms of this Chinese medicine remain a matter of considerable debate. Therefore, a series of experiments using ovalbumin-sensitized guinea pigs was performed to elucidate the possible antiasthmatic effect of XQLT. METHODS: The effect of XQLT on ovalbumin-induced airway inflammation in a guinea pig model of allergic asthma was examined, and early and late asthmatic responses were measured in terms of airway resistance and extent of eosinophil infiltration. Furthermore, the bronchorelaxing effect of XQLT was measured in isolated guinea pig trachea. RESULTS: XQLT significantly inhibited the antigen-induced immediate asthmatic response (IAR) and late asthmatic response (LAR) in actively sensitized guinea pigs. Cumulative administration of XQLT caused concentration-dependent relaxation of the carbachol-precontracted guinea pig trachea. The bronchorelaxing effect of XQLT was reversed by ICI-118551, a selective beta2-adrenoceptor antagonist. Furthermore, examination of bronchoalveolar lavage fluid (BALF) revealed that XQLT significantly suppressed the increase in eosinophils (24 h after antigen challenge) in the airway. In addition, XQLT significantly attenuated the increase in eosinophils at 1, 6, 24, 48, and 72 h after antigen challenge when it was administered once daily from the day of sensitization to the day of challenge. Histopathologic examination results showed that XQLT suppressed eosinophil infiltration into lung tissue. CONCLUSIONS: These results demonstrate that the antiasthmatic effects of XQLT appear to be partly mediated by stimulation of beta2-adrenoceptors, leading to bronchorelaxation, and that XQLT inhibits the infiltration of eosinophils into the airway. Thus, XQLT may be useful for the prevention or treatment of asthma.     

Autophagy deficiency in myeloid cells exacerbates eosinophilic inflammation in chronic rhinosinusitis

1. Download high-res image (246KB)
2. Download full-size image

     

High-sensitivity C-reactive protein: a predicative marker in severe asthma

Background and objective:   Serum levels of high-sensitivity CRP (hs-CRP) are associated with asthma but the relationship between higher levels of hs-CRP and the degree of asthma severity remains unclear. This study investigated whether hs-CRP is associated with asthma severity as well as with other clinical indices of asthma activity (pulmonary function, total serum IgE, and peripheral blood eosinophil counts).
Methods:   Levels of hs-CRP and clinical indices of asthma were determined among 177 control subjects and 281 asthmatic patients (84 intermittent, 30 mild, 63 moderate and 104 severe).
Results:   The level of hs-CRP was examined as both a continuous variable and by quartiles (<0.23, 0.23–0.51, 0.51–1.42 and ≥1.42 mg/L) in the five groups. Compared with the first quartile of hs-CRP, patients with higher levels were at increased risk of severe asthma independently of other clinical indices (adjusted OR 3.49, 95% CI: 1.51–8.12 for the third quartile; adjusted OR 6.46, 95% CI: 2.85–16.62 for fourth quartile, respectively).
Conclusions:   These findings suggest that hs-CRP might be a sensitive marker for severe asthma.     

辛芷鼻敏胶囊对变应性鼻炎大鼠骨髓及外周血IL-5 及嗜酸性粒细胞的影响

目的:探讨辛芷鼻敏胶囊对变应性鼻炎(AR)大鼠骨髓及外周血中IL-5和外周血及骨髓中嗜酸性粒细胞(EOS)的影响。方法:用卵清蛋白(OVA)致敏SD大鼠制成模型,分正常对照组、模型组、鼻炎康组、辛芷鼻敏胶囊组,分别ig生理盐水、鼻炎康片(3 g·kg-1)和辛芷鼻敏胶囊(3.6 g·kg-1),连续10 d。采用双抗体夹心酶联免疫吸附技术(ELISA)定量测定骨髓及外周血中白介素-5(IL-5)含量,骨髓和外周血涂片瑞氏染色计数EOS。结果:辛芷鼻敏胶囊组骨髓及外周血中IL-5,EOS比模型组有显著降低(P<0.05),且与鼻炎康组比较有统计学意义(P<0.05);辛芷鼻敏胶囊组大鼠鼻黏膜EOS计数明显低于模型组和鼻炎康组(P<0.01,P<0.05)。结论:辛芷鼻敏胶囊可以减少IL-5水平,减少嗜酸性粒细胞浸润,而起到一定的治疗作用。     

咳嗽变异性哮喘患儿尿白三烯E_4测定的意义

目的探讨咳嗽变异性哮喘(CVA)患儿尿白三烯E4(LTE4)测定的临床意义。方法收集咳嗽变异性哮喘46例作为病例组,并随机分成孟鲁司特治疗组(n=24)和常规治疗组(n=22);选取同期门诊健康体检儿童20例为对照组。检测两组急性发作期和缓解期尿LTE4水平和外周血嗜酸性粒细胞计数,并进行比较;对病例组患儿尿LTE4与外周血嗜酸性粒细胞计数进行相关性分析。结果孟鲁司特组、常规治疗组缓解期较急性期尿LTE4明显降低,P<0.01;缓解期孟鲁司特治疗组儿童尿LTE4水平显著低于常规治疗组,差异有统计学意义(P<0.01);两组与健康对照组比较,差异均有统计学意义;且孟鲁司特组、常规治疗组尿LTE4下降值有显著性差异(P<0.05)。急性期咳嗽变异哮喘儿童尿LTE4水平与外周血嗜酸性粒细胞计数无相关性(P>0.05)。结论白三烯在咳嗽变异性哮喘的发病机制中有重要作用,白三烯受体拮抗剂在咳嗽变异性哮喘治疗上的作用是显著的。测定CVA患儿尿LTE4水平可以为CVA的临床诊断和治疗监测提供依据。     

胎盘细胞嗜酸性粒细胞趋化因子和T淋巴细胞激活上调性表达分泌因子S表达与自然流产的相关性

目的 探讨嗜酸性粒细胞趋化因子(Eotaxin)、T淋巴细胞激活上调性表达分泌因子(RANTES)在正常妊娠免疫耐受及反复性自然流产中的作用.方法 构建正常妊娠小鼠模型CBA/J×BALB/c(正常妊娠组)和反复性自然流产小鼠模型CBA/J×DBA/2J(自然流产组);采用免疫组织化学方法检测胎盘细胞趋化因子Eotaxin、RANTES的表达;采用酶联免疫吸附试验(ELlSA)检测胎盘细胞培养上清液的白细胞介素(IL)-4,干扰素(IFN)-γ水平,分析胎盘细胞趋化因子Eotaxin、RANTES与细胞因子IL-4、IFN-γ水平的相关性.结果 自然流产组的胚胎丢失率为18.3%,显著高于正常妊娠组的3.6%(P<0.05).正常妊娠组的胎盘细胞Eotaxin的表达阳性率为53.6%.显著高于自然流产组的40.8%(P(0.05);而正常妊娠组的胎盘细胞RANTES的表达阳性率为44.3%,显著低于自然流产组的62.3%(P<0.05).正常妊娠组的IL-4水平为(1.33±0.43)ng/L,显著高于自然流产组的(0.78±0.41)ng/L(P=0.002);正常妊娠组的IFN-γ水平为(1.24±0.57)ng/L,显著低于自然流产组的(1.67±0.44)ng/L(P=0.038).自然流产组中,胎盘细胞Eotaxin的表达与IL-4水平呈正相关(r=0.752,P=0.003),RANTES的表达与]FN-γ水平亦呈正相关(r=0.658,P=0.014);正常妊娠组中,RANTES的表达与IFN-γ水平呈正相关(r=0.649,P=0.012),IL-4水平随Eotaxin表达阳性率的上升而显示出增加趋势.结论 Eotaxin、RANTES在妊娠免疫耐受形成和自然流产的发病机制中可能发挥重要作用.